FastQCFastQC Report
Fri 27 May 2016
SRR940230_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940230_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4250036
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA59750.039.9197461
GGTATCA36950.039.734341
TCAACGC68000.033.6731264
ATCAACG69150.033.319223
CAACGCA70600.032.4288445
TATCAAC73150.031.5625692
AACGCAG73450.031.1705426
ACGCAGA88350.025.8593377
GTACATG109750.025.5861471
CGCAGAG94150.024.3672038
TACATGG116100.023.9781572
ACATGGG121650.022.0248173
GTATACG2951.3278623E-1020.938391
TATAAGC20750.020.3759173
CATGGGG102700.019.9829034
GCAGAGT116900.019.6250849
CCTATAA22800.017.9219631
CTATAAG23350.017.9038412
AGAGTAC104350.017.61559710-11
CAGAGTA107850.017.02190810-11
Produced by FastQC (version 0.11.5)