FastQCFastQC Report
Fri 27 May 2016
SRR940229_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940229_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956654
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51450.041.3620341
GGTATCA28100.039.3875431
ATCAACG57400.035.7472573
TCAACGC57700.035.5613944
CAACGCA60700.033.8816535
AACGCAG63700.032.3629846
TATCAAC64850.031.641792
ACGCAGA74550.027.652537
GTACATG99200.026.8633691
CGCAGAG80550.025.2982238
TACATGG103500.024.919932
ACATGGG105650.024.3219623
CATGGGG89250.020.8084344
GCAGAGT99900.020.065289
AGAGTAC89050.018.32381210-11
CAGAGTA90950.016.55692110-11
GTACCGT3753.2869139E-916.4668456
TTATAGG18700.016.255833
GCTTATA19950.016.191061
GTTCTAG9700.016.1603761
Produced by FastQC (version 0.11.5)