##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940229_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3956654 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16693347459748 34.0 31.0 34.0 30.0 34.0 2 31.78816393851977 34.0 31.0 34.0 30.0 34.0 3 32.198183869501854 34.0 31.0 34.0 30.0 34.0 4 35.70860858695251 37.0 35.0 37.0 35.0 37.0 5 35.773827077121226 37.0 35.0 37.0 35.0 37.0 6 35.85097660801273 37.0 37.0 37.0 35.0 37.0 7 35.82938285733349 37.0 37.0 37.0 35.0 37.0 8 35.82970080274899 37.0 37.0 37.0 35.0 37.0 9 37.58242393699323 39.0 38.0 39.0 35.0 39.0 10-11 37.52523773875603 39.0 38.0 39.0 35.0 39.0 12-13 37.43949496215742 39.0 38.0 39.0 35.0 39.0 14-15 38.87277318158222 41.0 39.0 41.0 36.0 41.0 16-17 38.80449870016433 41.0 39.0 41.0 36.0 41.0 18-19 38.72376647035601 41.0 39.0 41.0 35.0 41.0 20-21 38.64385235605641 41.0 39.0 41.0 35.0 41.0 22-23 38.53971171095577 40.0 39.0 41.0 34.5 41.0 24-25 38.42866384070985 40.0 38.5 41.0 34.0 41.0 26-27 38.27143439886328 40.0 38.0 41.0 34.0 41.0 28-29 38.11204833680175 40.0 38.0 41.0 34.0 41.0 30-31 37.96678228624489 40.0 38.0 41.0 33.5 41.0 32-33 38.0363996952981 40.0 38.0 41.0 34.0 41.0 34-35 38.10997271937349 40.0 38.0 41.0 33.5 41.0 36-37 38.25140017297444 40.0 38.0 41.0 34.0 41.0 38-39 38.177749178978 40.0 38.0 41.0 34.0 41.0 40-41 38.05338260560565 40.0 38.0 41.0 33.5 41.0 42-43 37.92622491125077 40.0 38.0 41.0 33.0 41.0 44-45 37.79122422632861 40.0 38.0 41.0 33.0 41.0 46-47 37.59226849251918 40.0 38.0 41.0 33.0 41.0 48-49 37.42043896180965 40.0 38.0 41.0 32.5 41.0 50-51 36.49700858351526 39.0 36.0 40.5 31.0 40.5 52-53 36.50294428069778 39.0 36.5 40.5 31.0 41.0 54-55 36.909395792505485 40.0 37.0 41.0 31.0 41.0 56-57 36.801869711124596 40.0 36.0 41.0 31.0 41.0 58-59 36.5494609839526 39.0 36.0 41.0 31.0 41.0 60-61 36.24759430569365 39.0 35.0 41.0 31.0 41.0 62-63 35.91390275722871 38.5 35.0 41.0 30.0 41.0 64-65 35.538038832811765 37.5 35.0 40.0 30.0 41.0 66-67 35.1429096403173 37.0 35.0 40.0 29.0 41.0 68-69 34.71378505676766 36.5 35.0 39.0 29.0 41.0 70-71 34.300525772534066 36.0 34.0 39.0 29.0 41.0 72-73 33.887884181937565 35.5 34.0 38.5 28.0 40.0 74-75 33.461943601841355 35.0 34.0 37.0 27.5 39.0 76-77 32.96565860952209 35.0 34.0 37.0 26.5 39.0 78-79 32.55298694300791 35.0 33.0 36.0 26.0 38.5 80-81 32.22011894393596 35.0 33.0 36.0 26.0 37.0 82-83 31.88347679630314 35.0 33.0 35.0 25.0 37.0 84-85 31.611120911760292 35.0 33.0 35.0 24.5 36.5 86-87 31.331914415564263 35.0 32.0 35.0 24.0 36.0 88-89 31.058966616742328 35.0 32.0 35.0 23.0 36.0 90-91 30.785269952843993 34.5 32.0 35.0 20.0 35.0 92-93 30.515148152959547 34.0 31.5 35.0 18.5 35.0 94-95 30.19101847166823 34.0 31.0 35.0 15.5 35.0 96-97 29.83318733455086 34.0 31.0 35.0 4.5 35.0 98-99 29.423113443834108 34.0 31.0 35.0 2.0 35.0 100-101 27.58791127553736 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.2152251517449173 1101 2 0.0883100144070994 1101 3 0.20130615697912901 1101 4 0.2054538206418357 1101 5 0.34068430042331244 1101 6 0.41970184140444644 1101 7 0.3700310342609683 1101 8 0.3853494834652196 1101 9 0.533837487871466 1101 10-11 0.5873588758640693 1101 12-13 0.6129906890632526 1101 14-15 0.6660737880126604 1101 16-17 0.7358460546918124 1101 18-19 0.7349904649604824 1101 20-21 0.7760990454300014 1101 22-23 0.7468024248273295 1101 24-25 0.782009455120388 1101 26-27 0.8352954100301062 1101 28-29 0.8583830464928823 1101 30-31 0.8668506416120039 1101 32-33 0.8601136927396524 1101 34-35 0.8093817033872881 1101 36-37 0.8904399815402257 1101 38-39 0.9397764701332392 1101 40-41 0.9691294879240573 1101 42-43 0.9662411385996634 1101 44-45 0.9238513836235214 1101 46-47 0.9243441992182682 1101 48-49 0.9738262835987044 1101 50-51 0.8583941832275457 1101 52-53 0.9885982203950121 1101 54-55 1.0632064999965039 1101 56-57 1.0948232173032153 1101 58-59 1.0531552907812696 1101 60-61 1.0694722085366593 1101 62-63 1.1786313996572275 1101 64-65 1.2111059174050283 1101 66-67 1.2598404226550173 1101 68-69 1.140744125769416 1101 70-71 1.0117777893610267 1101 72-73 1.1005509959994413 1101 74-75 1.0004645308599294 1101 76-77 1.2353063243160776 1101 78-79 1.1188072637416084 1101 80-81 1.0904436113219518 1101 82-83 1.048656006563892 1101 84-85 1.0506532396000097 1101 86-87 0.9456726424887947 1101 88-89 0.9725440635030118 1101 90-91 1.0799718004102274 1101 92-93 1.0889016237430127 1101 94-95 1.3052568258483461 1101 96-97 1.1407532723567861 1101 98-99 1.0362223732824347 1101 100-101 1.3522504430474136 1102 1 0.25150099226889466 1102 2 0.7215194640402949 1102 3 0.36332987801458216 1102 4 0.3454892621525758 1102 5 0.2758155632829755 1102 6 0.2533098733772192 1102 7 0.2110214575123237 1102 8 0.21252741277610454 1102 9 0.19506781460125922 1102 10-11 0.19175701653131227 1102 12-13 0.20522075725314437 1102 14-15 0.3161925547773592 1102 16-17 0.2930614722876115 1102 18-19 0.3398281234508218 1102 20-21 0.34680889903178524 1102 22-23 0.3989434583405611 1102 24-25 0.36654095928395236 1102 26-27 0.3532822460404006 1102 28-29 0.3306901918892535 1102 30-31 0.3583096684247451 1102 32-33 0.2605421687546894 1102 34-35 0.2761348193316593 1102 36-37 0.39415110629649774 1102 38-39 0.3454853542104104 1102 40-41 0.36745221034915687 1102 42-43 0.2090356855623554 1102 44-45 0.27500700309588666 1102 46-47 0.4193875046508353 1102 48-49 0.4075573913344357 1102 50-51 0.3502819553677199 1102 52-53 0.4401244137182232 1102 54-55 0.5136977257987851 1102 56-57 0.48215287721552613 1102 58-59 0.4691960754498581 1102 60-61 0.48061608598874983 1102 62-63 0.5057331135612841 1102 64-65 0.5220499650625499 1102 66-67 0.47808552924885817 1102 68-69 0.47693701807677513 1102 70-71 0.4839101510152517 1102 72-73 0.4341296729215287 1102 74-75 0.48951159589348237 1102 76-77 0.5683027478475182 1102 78-79 0.638001965828245 1102 80-81 0.5071393637269139 1102 82-83 0.44250518085209833 1102 84-85 0.2981184713702518 1102 86-87 0.2556622470304788 1102 88-89 0.12456309850285052 1102 90-91 0.049903610512675556 1102 92-93 0.12667618772625389 1102 94-95 0.12437208077945527 1102 96-97 -0.05734430490894127 1102 98-99 0.0679069761543829 1102 100-101 0.18876193191404056 1103 1 0.18007358463459155 1103 2 0.6314041196146043 1103 3 0.252817720088629 1103 4 0.22084200819497823 1103 5 0.09575106425295843 1103 6 0.10668700261641106 1103 7 0.14107074963058608 1103 8 0.11317006772910077 1103 9 0.13396644315506023 1103 10-11 0.06455826002221698 1103 12-13 0.08890132412336982 1103 14-15 0.008474178093457851 1103 16-17 0.040153286189770654 1103 18-19 0.04378987698154191 1103 20-21 0.0380216936928548 1103 22-23 0.020894200980954736 1103 24-25 0.023803916115880952 1103 26-27 0.06459192502249778 1103 28-29 -0.10000824527470087 1103 30-31 -0.028354168670126967 1103 32-33 -0.006572531549664973 1103 34-35 -0.03549580695413823 1103 36-37 0.01424687725433671 1103 38-39 -0.06056145700881643 1103 40-41 -0.06846776436391622 1103 42-43 0.011486225081860368 1103 44-45 -0.09630314838667431 1103 46-47 -0.14220306771559876 1103 48-49 -0.011026025093812564 1103 50-51 -0.1190918674543795 1103 52-53 0.023275671438447887 1103 54-55 0.04872698281079835 1103 56-57 0.05948518299611294 1103 58-59 0.15224438780851557 1103 60-61 0.13620424588724944 1103 62-63 -0.03561583727042006 1103 64-65 0.07859320048509488 1103 66-67 3.8239206296708517E-4 1103 68-69 0.03864788797729801 1103 70-71 -0.04176821302517908 1103 72-73 -0.08980736394654798 1103 74-75 0.048582980395899256 1103 76-77 0.16871860257371196 1103 78-79 0.1763726701472237 1103 80-81 0.10779309800133063 1103 82-83 -0.00300112241282946 1103 84-85 -0.14719095669779847 1103 86-87 -0.05009362338056178 1103 88-89 -0.043153116627159704 1103 90-91 -0.07504995409606252 1103 92-93 -0.09174883242290832 1103 94-95 -0.17172251272893035 1103 96-97 -0.2947551543887563 1103 98-99 -0.4274455472153811 1103 100-101 -0.2251791673718877 1104 1 0.24839522192174712 1104 2 0.7323054851711426 1104 3 0.4124765168163904 1104 4 0.23494125332869942 1104 5 0.24545448119775415 1104 6 0.2097362056656209 1104 7 0.18826515889395523 1104 8 -0.008615046297386186 1104 9 0.17434913353775272 1104 10-11 0.10706236890563758 1104 12-13 0.11169643355641057 1104 14-15 0.08528288335044465 1104 16-17 0.20131063053676712 1104 18-19 0.15199287440868403 1104 20-21 0.20795774380132315 1104 22-23 0.09809009714236794 1104 24-25 0.14526207852232176 1104 26-27 0.14377916929594647 1104 28-29 0.016194661876482996 1104 30-31 0.11306991456429927 1104 32-33 0.0710582490983569 1104 34-35 0.07481994646506251 1104 36-37 0.14233989069218467 1104 38-39 0.17591029325603813 1104 40-41 0.09491650850311117 1104 42-43 0.027179770085751898 1104 44-45 0.1056554415029467 1104 46-47 0.05991055120836819 1104 48-49 0.07676962865011916 1104 50-51 -0.05379327974199555 1104 52-53 0.11505475157959921 1104 54-55 0.20150685153894443 1104 56-57 0.11456028462293943 1104 58-59 0.1675125737663521 1104 60-61 0.27021610920946415 1104 62-63 0.15214261083285407 1104 64-65 0.21693270046252877 1104 66-67 0.24868520415888185 1104 68-69 0.2302169437704933 1104 70-71 0.17647081512763663 1104 72-73 0.10983790466424637 1104 74-75 0.11190216828406108 1104 76-77 0.2526490847800602 1104 78-79 0.23214744359440687 1104 80-81 0.010785015648423268 1104 82-83 -0.06770322504596393 1104 84-85 0.0055142684212192705 1104 86-87 0.1262212856929672 1104 88-89 -0.011118429420061915 1104 90-91 -0.11153259057870102 1104 92-93 -0.1273206774775133 1104 94-95 -0.13924686732224956 1104 96-97 -0.08570260050861478 1104 98-99 -0.09114329668899046 1104 100-101 0.0694510065686309 1105 1 -0.009042302999439755 1105 2 0.5653149328688052 1105 3 0.16147030471208978 1105 4 0.17155892927469552 1105 5 0.052049792524094585 1105 6 0.09401486347518784 1105 7 0.04851956867879181 1105 8 -0.09501126225855927 1105 9 0.008734719941735136 1105 10-11 0.022791379152117486 1105 12-13 -0.08156203997182843 1105 14-15 -0.2082789188972427 1105 16-17 -0.1332466550627558 1105 18-19 -0.19013284777536654 1105 20-21 -0.16291685835708591 1105 22-23 -0.13972650384005192 1105 24-25 -0.07570845591182263 1105 26-27 -0.004928159603615256 1105 28-29 0.13772682431397243 1105 30-31 -0.02435372301791716 1105 32-33 0.04094925176031694 1105 34-35 -0.0531229718730728 1105 36-37 -0.049678054516789416 1105 38-39 -0.23648014327496725 1105 40-41 -0.2532698820352266 1105 42-43 -0.10542072549907999 1105 44-45 -0.09645346969183066 1105 46-47 -0.02933560383254985 1105 48-49 -0.21435079507034516 1105 50-51 -0.20632178043799598 1105 52-53 0.015329822696735107 1105 54-55 -0.1480912118037665 1105 56-57 0.007630972352941967 1105 58-59 -0.031061820408275764 1105 60-61 -0.03597207728549989 1105 62-63 0.03630892346373571 1105 64-65 0.03791276286418821 1105 66-67 -0.036839659594448904 1105 68-69 -0.013394423486317919 1105 70-71 -0.03685747360371039 1105 72-73 -0.15036746219188046 1105 74-75 -0.11391999415995713 1105 76-77 -0.12158716052203289 1105 78-79 -0.011385611600580603 1105 80-81 0.09721523209643834 1105 82-83 -0.07731482332335915 1105 84-85 -0.18337563199846585 1105 86-87 -0.12556129261420068 1105 88-89 -0.08403413757971734 1105 90-91 -0.1328075957831878 1105 92-93 -0.07853529755632849 1105 94-95 -0.14573511953407703 1105 96-97 -0.20570784089074934 1105 98-99 -0.18008765662166937 1105 100-101 -0.22955309241520538 1106 1 -0.013759609198601197 1106 2 0.5526079158184025 1106 3 0.1280134702843796 1106 4 0.11305790041086539 1106 5 0.05233502083691377 1106 6 0.08387872980814137 1106 7 0.046111654671484814 1106 8 0.02904135860039503 1106 9 0.009793318981941468 1106 10-11 -0.0348052143412545 1106 12-13 -0.0027350800130321318 1106 14-15 -0.008478463859177054 1106 16-17 -0.051638368101883714 1106 18-19 -0.005692735001069593 1106 20-21 -0.038499757828589054 1106 22-23 -0.021549841463084363 1106 24-25 -0.05673547692351377 1106 26-27 -0.21621004327946736 1106 28-29 -0.05591888868534767 1106 30-31 -0.04740100021695781 1106 32-33 -0.018764731241866173 1106 34-35 -0.08067360213193098 1106 36-37 -0.10049648748902484 1106 38-39 -0.02075329220065214 1106 40-41 -0.17348099437714382 1106 42-43 -0.17827671468107553 1106 44-45 -0.07520600228953356 1106 46-47 -0.02574351375604067 1106 48-49 -0.0541906307584199 1106 50-51 -0.19909969746220924 1106 52-53 -0.017742432079657533 1106 54-55 0.05342667501049192 1106 56-57 -0.028696905185981336 1106 58-59 -0.0772187166545919 1106 60-61 -0.02463153994853684 1106 62-63 0.035060108405524204 1106 64-65 0.02459483398672546 1106 66-67 -0.09823183155126003 1106 68-69 -0.2989925121631032 1106 70-71 -0.10710659086355889 1106 72-73 -0.15627130541048473 1106 74-75 -0.06798119866827079 1106 76-77 -0.03863485477974393 1106 78-79 0.07674425801881313 1106 80-81 -0.05043381022557725 1106 82-83 -0.13047499988670452 1106 84-85 -0.14232757683441832 1106 86-87 -0.1905983888853271 1106 88-89 -0.22355717203121728 1106 90-91 -0.11925912330523047 1106 92-93 -0.16220877788285293 1106 94-95 -0.1408311068375241 1106 96-97 -0.19238455201815086 1106 98-99 -0.24276367503350826 1106 100-101 -0.3259331431258623 1107 1 0.18277332171701488 1107 2 0.6323926170984286 1107 3 0.23446014707045748 1107 4 0.09520708423245594 1107 5 0.1586071304292389 1107 6 0.09732433486712466 1107 7 0.10356281148319368 1107 8 0.037221938473052774 1107 9 -0.023526656664650147 1107 10-11 0.016269141701123147 1107 12-13 0.022533490570339154 1107 14-15 0.102286079025383 1107 16-17 -0.014493700177588664 1107 18-19 -0.011167093402136175 1107 20-21 -0.06587842932924559 1107 22-23 -0.027477878010891743 1107 24-25 -0.09325748909726883 1107 26-27 -0.006709447957426562 1107 28-29 -0.063665016143716 1107 30-31 -0.03466895852525198 1107 32-33 0.01063989020654077 1107 34-35 0.004863217172719203 1107 36-37 0.0673998684914423 1107 38-39 0.06290524060938907 1107 40-41 0.07155974943388799 1107 42-43 0.11578018759028907 1107 44-45 0.05713011953256597 1107 46-47 -0.014431043638850838 1107 48-49 0.06047501594116511 1107 50-51 -0.08675459052782486 1107 52-53 0.06963643352104754 1107 54-55 0.10235530417768501 1107 56-57 0.06321266089967992 1107 58-59 0.08592059116007533 1107 60-61 -0.02860217580554547 1107 62-63 0.02212069906915559 1107 64-65 0.03082581402488671 1107 66-67 -0.024509132705588854 1107 68-69 0.16743499785761884 1107 70-71 0.14019566418586749 1107 72-73 0.0749177588454728 1107 74-75 0.09494681036155583 1107 76-77 0.15657438752738528 1107 78-79 0.057638118216651435 1107 80-81 0.10904671171190472 1107 82-83 0.07668662845052054 1107 84-85 0.02840546533246524 1107 86-87 0.13459037955233555 1107 88-89 0.11411628338695223 1107 90-91 0.06218259041636287 1107 92-93 0.01265902524368201 1107 94-95 0.12493116827054607 1107 96-97 0.09943688281853014 1107 98-99 0.04495162245798312 1107 100-101 0.2040079561093826 1108 1 0.13248417564251014 1108 2 0.25913826523997585 1108 3 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#Quality Count 2 24453.0 3 8939.0 4 6826.0 5 5215.0 6 4851.0 7 4478.0 8 4725.0 9 5283.0 10 6014.0 11 7022.0 12 7641.0 13 8494.0 14 9233.0 15 10365.0 16 12025.0 17 14716.0 18 14011.0 19 12002.0 20 12326.0 21 13454.0 22 14168.0 23 15535.0 24 17617.0 25 20689.0 26 24188.0 27 28858.0 28 35129.0 29 42754.0 30 52822.0 31 66216.0 32 85217.0 33 114943.0 34 166130.0 35 262779.0 36 497732.0 37 1035477.0 38 1126587.0 39 157735.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.29358304493327 18.01315301471921 13.640848825276967 25.05241511507055 2 16.560019841896896 19.76534074690786 37.993364291209666 25.68127511998558 3 18.752608749622986 23.203138766645118 28.97718811995331 29.067064363778584 4 13.609040081625471 16.114609361648768 35.50075992990241 34.77559062682335 5 15.339275322089465 35.931714657124694 33.7605275385797 14.968482482206142 6 34.23124987201213 34.15184050979749 17.31713156495602 14.299778053234357 7 30.174702860022062 30.607320161832043 20.76373437317894 18.454242604966957 8 27.17068146585592 33.808018744381286 20.004970254317243 19.016329535445546 9 26.79621984178551 15.89757267930596 19.440409163192506 37.86579831571602 10-11 25.759443121598995 25.29832274937603 27.350329260648977 21.591904868375998 12-13 26.310435882883098 22.385568576690638 27.478507964156556 23.82548757626971 14-15 23.539664259891747 23.97597042350315 24.66853915044549 27.815826166159614 16-17 24.128246136139904 27.026471472786902 25.343751663181962 23.501530727891232 18-19 24.134603447199073 26.30134710655409 26.42337229997767 23.140677146269166 20-21 24.461386592752003 25.722578284634668 26.31929151966291 23.496743602950417 22-23 24.483990902690877 25.33509066653553 26.18022365545617 24.000694775317424 24-25 24.39010086793338 25.57912359355157 26.055316597507016 23.975458941008032 26-27 24.161553977684246 25.87876777728032 25.993059096524718 23.966619148510713 28-29 24.13904864209738 25.86131161636342 26.077517255432227 23.922122486106982 30-31 24.213999491098587 25.657846088038426 25.996696564940734 24.131457855922246 32-33 24.1064484974743 25.65910895144286 25.966339176910203 24.268103374172636 34-35 24.47537949656658 25.641174745401035 25.796626753370212 24.086819004662168 36-37 24.284070232425144 25.53939810096676 25.86832975129324 24.308201915314857 38-39 24.186674600829296 25.729045986881836 26.092453554687705 23.991825857601164 40-41 24.43189387246445 25.468624202002278 26.033185077332 24.066296848201276 42-43 24.3283472677751 25.5009637198956 25.889661971296945 24.28102704103235 44-45 24.279967823858374 25.64521657971255 25.743062082661943 24.331753513767133 46-47 24.319566482145536 25.6470341694609 25.660596137607083 24.37280321078648 48-49 24.454678125670696 25.709107400481773 25.600152991203007 24.236061482644523 50-51 24.15835306662568 25.71135285149129 25.785891481765276 24.344402600117753 52-53 24.524890332567605 25.63089980322244 25.496276496300137 24.347933367909825 54-55 24.359148735640495 25.4650192985379 25.71740326903353 24.458428696788076 56-57 24.286181102237787 25.77552626339558 25.80559936036204 24.132693274004595 58-59 24.422373074524245 25.520414739052512 25.7096472690472 24.347564917376047 60-61 24.394684815419698 25.430126963741255 25.970658279947212 24.20452994089183 62-63 24.433275608390513 25.53752902944351 25.83868560570674 24.19050975645923 64-65 24.285853154259964 25.51191252849085 25.72871540220656 24.473518915042625 66-67 24.17967638057515 25.71700263472303 25.68880235127921 24.414518633422606 68-69 24.055119035851693 25.474516622649535 25.99454401632902 24.47582032516975 70-71 24.04817426917484 25.540283667017967 25.896501320441285 24.51504074336591 72-73 24.233203002311075 25.441344404413353 25.79088095724102 24.534571636034556 74-75 24.06235025267205 25.582086152580573 25.757368342749682 24.598195251997694 76-77 24.27702504607269 25.43213890949979 25.825740099317517 24.46509594511 78-79 24.096037852690067 25.560387451082907 25.82611572186838 24.51745897435865 80-81 24.018439785341076 25.61222729171657 25.93005698915013 24.43927593379222 82-83 24.09076251907603 25.566712724274872 25.784950197235357 24.557574559413744 84-85 24.061052788852553 25.44444712702536 25.77853378806872 24.715966296053367 86-87 24.14278728161342 25.383724482566805 25.98493023093556 24.48855800488422 88-89 24.01961137493116 25.304313675822023 25.940730297205512 24.73534465204131 90-91 23.92413096699636 25.475755098759656 26.046386935809668 24.553726998434318 92-93 24.023335600836223 25.34425611514684 26.087804066512128 24.544604217504812 94-95 23.9448348599373 25.252348078350632 26.01655637095275 24.786260690759317 96-97 24.10313518517816 25.21611390831332 25.934503731640596 24.74624717486793 98-99 23.827641084059607 25.127996087362508 25.993061316326294 25.05130151225159 100-101 23.73488424625144 25.052371009473262 26.327374111324392 24.885370632950902 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 291.0 1 186.0 2 132.5 3 156.5 4 121.0 5 129.5 6 189.0 7 397.5 8 663.5 9 831.0 10 855.0 11 845.0 12 904.5 13 975.5 14 1022.5 15 1077.0 16 1138.0 17 1196.5 18 1242.5 19 1307.5 20 1495.5 21 1722.0 22 2019.0 23 2423.5 24 2941.0 25 3723.0 26 4931.5 27 6773.5 28 9240.0 29 12517.5 30 17244.0 31 22743.5 32 28460.0 33 35135.5 34 42878.5 35 50796.0 36 58999.0 37 67927.5 38 77292.5 39 84169.0 40 90212.0 41 100939.0 42 112793.0 43 124610.0 44 136682.5 45 145743.0 46 153111.5 47 161760.5 48 173621.0 49 181884.5 50 184395.5 51 184632.0 52 180086.5 53 177918.5 54 177137.0 55 169085.0 56 157947.5 57 145108.5 58 130350.0 59 114471.5 60 96028.0 61 76363.5 62 59749.5 63 46154.5 64 34918.5 65 26130.5 66 18858.0 67 14115.5 68 10010.0 69 6704.0 70 4904.0 71 3571.0 72 2641.0 73 1966.5 74 1465.5 75 1113.5 76 766.0 77 455.0 78 291.0 79 205.0 80 136.0 81 97.5 82 78.0 83 76.5 84 64.5 85 44.5 86 35.0 87 40.0 88 45.5 89 35.5 90 26.0 91 22.5 92 19.5 93 15.5 94 14.0 95 12.0 96 15.5 97 13.0 98 8.0 99 5.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.030606669170465756 2 0.035054872121747314 3 0.030707764692085786 4 0.02593100129553911 5 0.027194695315789552 6 0.030353930366415663 7 0.03020228708398561 8 0.028357293814419964 9 0.03194618483193123 10-11 0.01884167784193412 12-13 0.028281472173204935 14-15 0.023896453922935896 16-17 0.038150922471360905 18-19 0.04379963474188039 20-21 0.04607428397833118 22-23 0.03585099935450509 24-25 0.01491158943895524 26-27 0.02459148563407364 28-29 0.029330338210012803 30-31 0.027156784495182038 32-33 0.033209878852181664 34-35 0.035737266892682557 36-37 0.0321736497555763 38-39 0.019334518509831793 40-41 0.016655487186900853 42-43 0.015897270774750585 44-45 0.02345416101584824 46-47 0.018664760679099057 48-49 0.022013549832762734 50-51 0.024945319959743764 52-53 0.02963362477487291 54-55 0.029646261715075416 56-57 0.032641216543068965 58-59 0.03040447812722568 60-61 0.0301770132035806 62-63 0.03544661726802495 64-65 0.03077094939309831 66-67 0.025779358013109054 68-69 0.025198058763793853 70-71 0.03405655384574947 72-73 0.01772962710411373 74-75 0.026095281518171667 76-77 0.04454521421382815 78-79 0.02804137030935735 80-81 0.027207332255992058 82-83 0.02781390538571227 84-85 0.027636988222877207 86-87 0.02157125692567508 88-89 0.025033778541161296 90-91 0.021823995729725168 92-93 0.03779708814569078 94-95 0.07301624049007065 96-97 0.0833785314561243 98-99 0.08366918108078189 100-101 0.08516034002467741 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3956654.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.773646883023076 #Duplication Level Percentage of deduplicated Percentage of total 1 78.12118491056181 41.227398265533395 2 11.133131149829884 11.750718640070138 3 3.897375886623813 6.170362164332821 4 1.9571195164545232 4.131373370769754 5 1.1575864044249908 3.05450280718564 6 0.755641929171786 2.39267882040709 7 0.5091087360259513 1.8807267262067031 8 0.3888321238646677 1.641607136128789 9 0.2920902739276469 1.3873202076800806 >10 1.6228075564591036 15.306979863743969 >50 0.10520044419214851 3.8140912817120105 >100 0.05583580758674369 5.328576650656977 >500 0.002979837383536383 1.0756624516076574 >1k 0.0011054234933927582 0.8380016139649994 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.527388040500888E-5 2 0.0 0.0 0.0 0.0 2.527388040500888E-5 3 0.0 0.0 0.0 0.0 2.527388040500888E-5 4 0.0 0.0 0.0 2.527388040500888E-5 2.527388040500888E-5 5 0.0 0.0 0.0 2.527388040500888E-5 2.527388040500888E-5 6 0.0 0.0 0.0 2.527388040500888E-5 5.054776081001776E-5 7 0.0 0.0 0.0 2.527388040500888E-5 5.054776081001776E-5 8 0.0 0.0 0.0 2.527388040500888E-5 5.054776081001776E-5 9 0.0 0.0 0.0 2.527388040500888E-5 5.054776081001776E-5 10-11 0.0 0.0 0.0 3.791082060751332E-5 5.054776081001776E-5 12-13 0.0 0.0 0.0 5.054776081001776E-5 6.31847010125222E-5 14-15 1.263694020250444E-5 0.0 0.0 5.054776081001776E-5 1.263694020250444E-4 16-17 2.527388040500888E-5 0.0 0.0 5.054776081001776E-5 1.7691716283506215E-4 18-19 3.791082060751332E-5 0.0 0.0 6.31847010125222E-5 2.0219104324007103E-4 20-21 5.054776081001776E-5 0.0 0.0 7.582164121502664E-5 2.0219104324007103E-4 22-23 5.054776081001776E-5 0.0 0.0 1.5164328243005327E-4 2.0219104324007103E-4 24-25 5.054776081001776E-5 0.0 0.0 1.5164328243005327E-4 2.0219104324007103E-4 26-27 5.054776081001776E-5 0.0 0.0 2.4010186384758434E-4 2.274649236450799E-4 28-29 7.582164121502664E-5 0.0 0.0 7.20305591542753E-4 2.274649236450799E-4 30-31 1.0109552162003552E-4 0.0 0.0 0.0025147511002983833 2.274649236450799E-4 32-33 1.0109552162003552E-4 0.0 0.0 0.0072030559154275305 2.274649236450799E-4 34-35 1.3900634222754882E-4 0.0 0.0 0.013028685348782078 2.274649236450799E-4 36-37 1.7691716283506215E-4 0.0 0.0 0.023529982657063266 2.274649236450799E-4 38-39 2.527388040500888E-4 0.0 0.0 0.04007173738214158 2.527388040500888E-4 40-41 2.527388040500888E-4 0.0 0.0 0.05719479135653509 2.527388040500888E-4 42-43 2.527388040500888E-4 0.0 0.0 0.07700951359406205 2.527388040500888E-4 44-45 2.7801268445509764E-4 0.0 0.0 0.10180319027137577 2.527388040500888E-4 46-47 3.285604452651154E-4 0.0 0.0 0.1301352102053907 2.6537574425259324E-4 48-49 3.285604452651154E-4 0.0 0.0 0.16037540810998385 2.7801268445509764E-4 50-51 3.285604452651154E-4 0.0 0.0 0.19319354181588788 3.0328656486010655E-4 52-53 3.285604452651154E-4 0.0 0.0 0.22870334378492535 3.285604452651154E-4 54-55 3.285604452651154E-4 0.0 0.0 0.2701145968285324 3.285604452651154E-4 56-57 3.285604452651154E-4 0.0 0.0 0.3197904087645773 3.285604452651154E-4 58-59 3.538343256701243E-4 0.0 0.0 0.3748495572268892 3.285604452651154E-4 60-61 3.538343256701243E-4 0.0 0.0 0.4326256478327395 3.285604452651154E-4 62-63 3.538343256701243E-4 0.0 0.0 0.4950521324331114 3.285604452651154E-4 64-65 3.538343256701243E-4 0.0 0.0 0.5707600411863155 3.285604452651154E-4 66-67 3.7910820607513316E-4 0.0 0.0 0.6612784438568549 3.285604452651154E-4 68-69 4.0438208648014206E-4 0.0 0.0 0.7621338636130428 3.285604452651154E-4 70-71 4.0438208648014206E-4 0.0 0.0 0.8728840075477916 3.4119738546761985E-4 72-73 4.4229290708765537E-4 0.0 0.0 0.9908372073979681 3.7910820607513316E-4 74-75 4.802037276951687E-4 0.0 0.0 1.1280870149373687 3.7910820607513316E-4 76-77 4.802037276951687E-4 2.527388040500888E-5 0.0 1.2917480275000037 3.7910820607513316E-4 78-79 4.802037276951687E-4 2.527388040500888E-5 2.527388040500888E-5 1.4746677369312555 3.7910820607513316E-4 80-81 5.560253689101953E-4 2.527388040500888E-5 2.527388040500888E-5 1.6754434428686462 3.917451462776376E-4 82-83 5.560253689101953E-4 2.527388040500888E-5 2.527388040500888E-5 1.8912191968264094 4.296559668851509E-4 84-85 5.939361895177086E-4 2.527388040500888E-5 2.527388040500888E-5 2.128920042035518 4.296559668851509E-4 86-87 6.31847010125222E-4 2.527388040500888E-5 2.527388040500888E-5 2.3970632761924597 4.4229290708765537E-4 88-89 6.31847010125222E-4 2.527388040500888E-5 2.527388040500888E-5 2.699667446281631 4.549298472901598E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5145 0.0 41.362034 1 GGTATCA 2810 0.0 39.387543 1 ATCAACG 5740 0.0 35.747257 3 TCAACGC 5770 0.0 35.561394 4 CAACGCA 6070 0.0 33.881653 5 AACGCAG 6370 0.0 32.362984 6 TATCAAC 6485 0.0 31.64179 2 ACGCAGA 7455 0.0 27.65253 7 GTACATG 9920 0.0 26.863369 1 CGCAGAG 8055 0.0 25.298223 8 TACATGG 10350 0.0 24.91993 2 ACATGGG 10565 0.0 24.321962 3 CATGGGG 8925 0.0 20.808434 4 GCAGAGT 9990 0.0 20.06528 9 AGAGTAC 8905 0.0 18.323812 10-11 CAGAGTA 9095 0.0 16.556921 10-11 GTACCGT 375 3.2869139E-9 16.466845 6 TTATAGG 1870 0.0 16.25583 3 GCTTATA 1995 0.0 16.19106 1 GTTCTAG 970 0.0 16.160376 1 >>END_MODULE