FastQCFastQC Report
Fri 27 May 2016
SRR940229_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940229_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3956654
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA56200.040.4093361
GGTATCA33800.038.5144731
ATCAACG61100.035.994083
TCAACGC62200.035.4338954
CAACGCA63450.034.6596535
TATCAAC68050.032.737612
AACGCAG67750.032.6000676
ACGCAGA77250.028.713977
GTACATG99700.026.7336221
CGCAGAG83550.026.4351228
TACATGG103950.025.2698632
ACATGGG107450.023.7388213
CATGGGG89350.021.9556774
GCAGAGT105150.020.9144559
AGAGTAC93650.018.25868610-11
CAGAGTA97750.017.44425810-11
CGCGGAA5750.016.10810382-83
ATGGGGA58050.015.9553825
GTACTTT47900.015.91649114-15
GAGTACT47300.015.71668612-13
Produced by FastQC (version 0.11.5)