FastQCFastQC Report
Fri 27 May 2016
SRR940228_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940228_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000138
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA53000.038.4450951
GGTATCA33150.037.251981
ATCAACG57550.034.0863533
TCAACGC57900.033.7158134
CAACGCA60000.032.5361675
TATCAAC63750.030.8469542
AACGCAG63800.030.5230646
GTACATG99700.028.0118431
ACGCAGA75100.025.9936267
TACATGG105650.025.6269822
ACATGGG109450.024.2155463
CGCAGAG82450.023.6188188
CATGGGG89000.021.9342214
GCAGAGT101100.018.9839929
TATAAGC22100.018.2684823
CCTATAA22350.017.8509461
CTATAAG21700.017.7303542
ATGGGGA53500.017.4011575
AGAGTAC90800.016.92651210-11
CAGAGTA91950.016.43063710-11
Produced by FastQC (version 0.11.5)