##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940228_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4000138 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37285988633392 34.0 31.0 34.0 31.0 34.0 2 32.382654798409455 34.0 31.0 34.0 31.0 34.0 3 32.492187519530574 34.0 31.0 34.0 31.0 34.0 4 35.87564279032373 37.0 37.0 37.0 35.0 37.0 5 35.8744388318603 37.0 37.0 37.0 35.0 37.0 6 35.94676908646652 37.0 37.0 37.0 35.0 37.0 7 35.90525501870186 37.0 37.0 37.0 35.0 37.0 8 35.89384866222115 37.0 37.0 37.0 35.0 37.0 9 37.654114183060685 39.0 39.0 39.0 35.0 39.0 10-11 37.58647589158174 39.0 38.0 39.0 35.0 39.0 12-13 37.5266340811242 39.0 38.0 39.0 35.0 39.0 14-15 38.98081791178204 41.0 39.0 41.0 36.0 41.0 16-17 38.893413802223826 41.0 39.0 41.0 36.0 41.0 18-19 38.842160820451696 41.0 39.0 41.0 36.0 41.0 20-21 38.76705253668749 41.0 39.0 41.0 35.5 41.0 22-23 38.657178077356335 40.0 39.0 41.0 35.0 41.0 24-25 38.542127546599644 40.0 38.5 41.0 35.0 41.0 26-27 38.38673565761981 40.0 38.0 41.0 34.0 41.0 28-29 38.22893385178212 40.0 38.0 41.0 34.0 41.0 30-31 38.196521595004974 40.0 38.0 41.0 34.0 41.0 32-33 38.21590817616792 40.0 38.0 41.0 34.0 41.0 34-35 38.2486266723798 40.0 38.0 41.0 34.5 41.0 36-37 38.401676767151535 40.5 39.0 41.0 35.0 41.0 38-39 38.3294306346431 40.5 38.0 41.0 34.0 41.0 40-41 38.2162504143607 40.0 38.0 41.0 34.0 41.0 42-43 38.10136287798071 40.0 38.0 41.0 34.0 41.0 44-45 37.953922589670654 40.0 38.0 41.0 33.0 41.0 46-47 37.78293936359196 40.0 38.0 41.0 33.0 41.0 48-49 37.60646295202815 40.0 38.0 41.0 33.0 41.0 50-51 36.663600605779095 39.0 36.5 40.0 31.5 40.5 52-53 36.747318592508556 39.5 36.5 40.5 31.5 41.0 54-55 37.01464311981237 40.0 37.0 41.0 32.0 41.0 56-57 36.95293699867354 40.0 36.0 41.0 32.0 41.0 58-59 36.72483174330486 39.0 36.0 41.0 31.5 41.0 60-61 36.41928065981724 39.0 35.0 41.0 31.0 41.0 62-63 36.07414519199088 39.0 35.0 41.0 31.0 41.0 64-65 35.687480031938904 38.0 35.0 40.0 30.0 41.0 66-67 35.29547343116663 37.0 35.0 40.0 30.0 41.0 68-69 34.89125362674987 36.5 35.0 39.0 29.0 41.0 70-71 34.47513348289484 36.0 34.0 39.0 29.0 41.0 72-73 34.05310604284152 35.5 34.0 38.5 29.0 40.0 74-75 33.62732973212424 35.0 34.0 37.0 28.5 39.0 76-77 33.19990622823513 35.0 34.0 37.0 27.5 39.0 78-79 32.803941514017765 35.0 33.5 36.0 26.5 38.5 80-81 32.414784814923884 35.0 33.0 36.0 26.0 37.0 82-83 32.07441405771501 35.0 33.0 35.5 26.0 37.0 84-85 31.7549284549683 35.0 33.0 35.0 25.0 36.5 86-87 31.484919395280862 35.0 32.5 35.0 24.5 36.0 88-89 31.199932352333846 35.0 32.0 35.0 23.5 36.0 90-91 30.944472915684408 34.5 32.0 35.0 23.0 35.0 92-93 30.642445085644546 34.0 32.0 35.0 20.0 35.0 94-95 30.31741629913768 34.0 31.0 35.0 18.0 35.0 96-97 29.977120039358642 34.0 31.0 35.0 8.0 35.0 98-99 29.539533386098178 34.0 31.0 35.0 2.0 35.0 100-101 27.59065562238103 32.5 27.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1687944886944024 1101 2 0.21183887895248432 1101 3 0.2208879189363273 1101 4 0.1619299330682935 1101 5 0.10587589507096595 1101 6 0.09036572667745446 1101 7 0.04987357252930735 1101 8 0.11434372917602076 1101 9 0.1251446028241432 1101 10-11 0.18306629768861882 1101 12-13 0.17598885261651986 1101 14-15 0.22452204074202342 1101 16-17 0.20755703929584968 1101 18-19 0.23963407164433903 1101 20-21 0.1768407584729772 1101 22-23 0.26557855175027356 1101 24-25 0.2249496162148077 1101 26-27 0.28099903298421935 1101 28-29 0.21724449227035336 1101 30-31 0.18942583955885794 1101 32-33 0.22112145155281837 1101 34-35 0.18317172495483192 1101 36-37 0.2510766103814248 1101 38-39 0.3296245506087345 1101 40-41 0.24859219138625832 1101 42-43 0.23813173529462972 1101 44-45 0.2716868276088107 1101 46-47 0.29207252724021515 1101 48-49 0.2692290446693164 1101 50-51 0.16237215962615892 1101 52-53 0.28981050024600563 1101 54-55 0.43120620731799875 1101 56-57 0.41018823361379475 1101 58-59 0.38704500074703674 1101 60-61 0.3529127761281643 1101 62-63 0.3357499374945263 1101 64-65 0.33026629691832454 1101 66-67 0.21558015536504627 1101 68-69 0.2400233560268532 1101 70-71 0.2754120896373493 1101 72-73 0.2759557379341331 1101 74-75 0.32796191492015936 1101 76-77 0.2873820836166914 1101 78-79 0.2914854502988504 1101 80-81 0.2130805014283368 1101 82-83 0.1272764024306028 1101 84-85 0.17736128628864734 1101 86-87 0.1949761670744934 1101 88-89 0.32174519418728664 1101 90-91 0.27329893301160624 1101 92-93 0.2541945932705971 1101 94-95 0.25653732948745045 1101 96-97 0.20678029307670798 1101 98-99 0.11531104564669192 1101 100-101 0.46524880743533714 1102 1 0.2043943290715191 1102 2 0.30613269449996494 1102 3 0.2714588109826792 1102 4 0.2479209562830107 1102 5 0.24114034054160527 1102 6 0.22095392899812083 1102 7 0.22939553135300628 1102 8 0.28003955682601855 1102 9 0.2853525263119323 1102 10-11 0.36083090331599266 1102 12-13 0.35012831043257364 1102 14-15 0.3986078126841619 1102 16-17 0.43130334568103024 1102 18-19 0.38003838066143913 1102 20-21 0.40414881729321195 1102 22-23 0.3216589023867584 1102 24-25 0.41350291370861925 1102 26-27 0.29697653630079657 1102 28-29 0.34797062958340064 1102 30-31 0.3396108873255983 1102 32-33 0.34641357077349966 1102 34-35 0.29920354809908645 1102 36-37 0.33336891378235833 1102 38-39 0.3968201793974444 1102 40-41 0.4985941599795254 1102 42-43 0.403221736709348 1102 44-45 0.4000800484409339 1102 46-47 0.32955854689145525 1102 48-49 0.3904378343381367 1102 50-51 0.2994503801780084 1102 52-53 0.3612432757890076 1102 54-55 0.5290646447754597 1102 56-57 0.4349750916855655 1102 58-59 0.45308319679489983 1102 60-61 0.42814203382837235 1102 62-63 0.4073154763018394 1102 64-65 0.3433316502107502 1102 66-67 0.34352253497389285 1102 68-69 0.3845061565276069 1102 70-71 0.3538064115470618 1102 72-73 0.2938638640201958 1102 74-75 0.2690136909249432 1102 76-77 0.2994117232907172 1102 78-79 0.3989157554241345 1102 80-81 0.3242214380249493 1102 82-83 0.3364870165516294 1102 84-85 0.3046654746543247 1102 86-87 0.33902103898913794 1102 88-89 0.2989454808678502 1102 90-91 0.3423722994795888 1102 92-93 0.34170890727924075 1102 94-95 0.3175106687287865 1102 96-97 0.3895337477910559 1102 98-99 0.4359451729125752 1102 100-101 0.7666309601020842 1103 1 0.07942543972969673 1103 2 0.15998218395230168 1103 3 0.05947545915365282 1103 4 0.0411451189454084 1103 5 0.1096534482428595 1103 6 0.06896607316517134 1103 7 0.06221952901368866 1103 8 0.11134022046884695 1103 9 0.09661344512873171 1103 10-11 0.13753239386219462 1103 12-13 0.11854284343726107 1103 14-15 0.12243958826544343 1103 16-17 0.090031332009751 1103 18-19 0.07145646719464338 1103 20-21 0.10874300372260848 1103 22-23 0.03974448819149501 1103 24-25 0.08703659669331643 1103 26-27 0.05556909524589315 1103 28-29 0.12167560682781442 1103 30-31 0.08559908473565514 1103 32-33 0.12169440527657827 1103 34-35 0.06617204362862594 1103 36-37 0.14592454168762004 1103 38-39 0.15679671453559507 1103 40-41 0.23415353345439627 1103 42-43 0.1619686006781862 1103 44-45 0.18280791672838603 1103 46-47 0.15767408086556856 1103 48-49 0.2657233034330915 1103 50-51 0.16901612916134212 1103 52-53 0.18632552059071372 1103 54-55 0.25485223093149045 1103 56-57 0.250144687021681 1103 58-59 0.32951393499576653 1103 60-61 0.3242748445879968 1103 62-63 0.35054124570810785 1103 64-65 0.34432824749850965 1103 66-67 0.3530616965070905 1103 68-69 0.4208887909935015 1103 70-71 0.350295388131876 1103 72-73 0.2597365602311541 1103 74-75 0.24663949448891032 1103 76-77 0.21332994041186737 1103 78-79 0.14808405742745379 1103 80-81 0.1340174353251129 1103 82-83 0.15115762509658737 1103 84-85 0.1274175567639304 1103 86-87 0.039302142349754376 1103 88-89 0.09841507615275447 1103 90-91 0.11182481462907035 1103 92-93 0.27035501240407456 1103 94-95 0.2140853340523634 1103 96-97 0.05994506653984644 1103 98-99 0.09795469354214603 1103 100-101 0.45736233966901096 1104 1 0.1075540157766639 1104 2 0.15438366594313635 1104 3 0.16027444105468192 1104 4 0.1554631619785667 1104 5 0.1533562700806712 1104 6 0.2039385937298448 1104 7 0.15835650027189274 1104 8 0.11945832815949586 1104 9 0.17722492029460568 1104 10-11 0.23794137817505856 1104 12-13 0.19550835430845126 1104 14-15 0.17017965487292486 1104 16-17 0.17282114896745782 1104 18-19 0.30478150452665176 1104 20-21 0.3177945398512705 1104 22-23 0.2572643601080742 1104 24-25 0.26013394727549155 1104 26-27 0.28307752381218876 1104 28-29 0.2982008309481401 1104 30-31 0.27295957079581257 1104 32-33 0.21897058001601977 1104 34-35 0.24673066385820874 1104 36-37 0.22380346796267503 1104 38-39 0.19198886532097248 1104 40-41 0.21890514091155922 1104 42-43 0.2796465242489248 1104 44-45 0.23457881367152567 1104 46-47 0.16444387452605724 1104 48-49 0.2914695586865079 1104 50-51 0.22106926120118686 1104 52-53 0.14095238013513267 1104 54-55 0.3733884057735679 1104 56-57 0.23069868885877298 1104 58-59 0.19582430123429617 1104 60-61 0.2964734046758508 1104 62-63 0.24271332701234627 1104 64-65 0.32143128383499686 1104 66-67 0.3383841885916752 1104 68-69 0.25120263642104135 1104 70-71 0.1986312273707611 1104 72-73 0.18251703777293216 1104 74-75 0.16011904270749966 1104 76-77 0.182071120565098 1104 78-79 0.18270660647221604 1104 80-81 0.06919701381982435 1104 82-83 0.09084013627052911 1104 84-85 0.06137839279560353 1104 86-87 0.09536044698918644 1104 88-89 0.16883786432761738 1104 90-91 0.0804851729136935 1104 92-93 0.12493337882733613 1104 94-95 0.16931515039429001 1104 96-97 0.12485400723455342 1104 98-99 0.18285229351751298 1104 100-101 0.3388492567893948 1105 1 -0.17073299319478963 1105 2 0.0061426345716313335 1105 3 -0.058907288930306834 1105 4 0.008525126496749635 1105 5 0.01722459015677913 1105 6 0.07380053718755164 1105 7 0.06291546336837683 1105 8 -0.01740591921282686 1105 9 0.06038183773424777 1105 10-11 0.008860070042643997 1105 12-13 0.02488249531442932 1105 14-15 0.07809231138438122 1105 16-17 0.06843401803690341 1105 18-19 0.09013702811675017 1105 20-21 -0.016857520029887496 1105 22-23 0.09172816768516157 1105 24-25 0.1317608584443022 1105 26-27 0.22152878633095696 1105 28-29 0.24138023797188168 1105 30-31 0.24425823691283455 1105 32-33 0.22257724789018596 1105 34-35 0.16680365492072724 1105 36-37 0.14397242008369204 1105 38-39 0.16231117922718852 1105 40-41 0.1927723121239211 1105 42-43 0.051333116887676056 1105 44-45 0.043519284695953786 1105 46-47 0.03645896698046869 1105 48-49 0.04992941766621328 1105 50-51 -0.012479058467299353 1105 52-53 0.05853105091554767 1105 54-55 0.3062635846996997 1105 56-57 0.1923559982101679 1105 58-59 0.19003964488588565 1105 60-61 0.2695648502266792 1105 62-63 0.3083364060472533 1105 64-65 0.2528782079676475 1105 66-67 0.3547662637938487 1105 68-69 0.3468105133317607 1105 70-71 0.2362936681518093 1105 72-73 0.27013184984151195 1105 74-75 0.1977399023851376 1105 76-77 0.1992870318548654 1105 78-79 0.2946560996558887 1105 80-81 0.33311621994648277 1105 82-83 0.34093063810318824 1105 84-85 0.42310235929440054 1105 86-87 0.39660777697282157 1105 88-89 0.43850414913154623 1105 90-91 0.36699944751542546 1105 92-93 0.32928381769450255 1105 94-95 0.3831572722522161 1105 96-97 0.22317969906708157 1105 98-99 0.07270213083864974 1105 100-101 0.24939504956421743 1106 1 -0.2089146089759666 1106 2 -0.15156999242121572 1106 3 -0.16676598774237306 1106 4 -0.02708991664169247 1106 5 0.020509444119298337 1106 6 0.07024436998582928 1106 7 0.0504717394809191 1106 8 0.032663782507661665 1106 9 -0.17131219900074512 1106 10-11 -0.15183146065918862 1106 12-13 0.04137884023595717 1106 14-15 -0.04255292739957639 1106 16-17 -0.16254685763259857 1106 18-19 0.029282926329223358 1106 20-21 -0.004371629342372785 1106 22-23 0.002177389882390912 1106 24-25 0.02196694446288916 1106 26-27 0.0375410021734055 1106 28-29 0.08007900546041213 1106 30-31 0.061824901720143544 1106 32-33 0.1465761990211547 1106 34-35 -0.12053790784593588 1106 36-37 0.005284640058484058 1106 38-39 -0.008884884465885534 1106 40-41 0.08657329856199425 1106 42-43 0.14495850269883448 1106 44-45 0.08416829078230847 1106 46-47 0.06886532746877805 1106 48-49 0.10242708373383635 1106 50-51 0.021874805800656816 1106 52-53 0.14727556938213837 1106 54-55 0.27114779647389753 1106 56-57 0.24465448638581933 1106 58-59 0.23419316384389788 1106 60-61 0.35956880065346297 1106 62-63 0.3006399409864784 1106 64-65 0.05921979952821488 1106 66-67 0.24569144434907741 1106 68-69 0.22957289413675142 1106 70-71 0.28157243828841416 1106 72-73 0.30261543581220707 1106 74-75 0.35390530900742334 1106 76-77 0.18862467455484477 1106 78-79 0.058250224206254586 1106 80-81 0.33315151958196054 1106 82-83 0.5194795977940174 1106 84-85 0.3761345678624757 1106 86-87 0.3099049448457194 1106 88-89 0.3796091168745477 1106 90-91 0.10409863551537768 1106 92-93 0.20808041095439123 1106 94-95 0.33070050798065154 1106 96-97 0.23525512607520582 1106 98-99 0.3046429035922458 1106 100-101 0.6493146194050254 1107 1 -0.021404271357617688 1107 2 0.10056050211154144 1107 3 0.037697571385024276 1107 4 0.07335428195777638 1107 5 0.04869983398573652 1107 6 0.04686185873141113 1107 7 0.0857389675058613 1107 8 0.03008433997820248 1107 9 -0.017889773906532014 1107 10-11 0.044487827375455424 1107 12-13 0.04968130642544821 1107 14-15 0.08893596609955523 1107 16-17 0.033436191981351726 1107 18-19 0.10995970834559898 1107 20-21 0.15822067672863227 1107 22-23 0.15984739467432263 1107 24-25 0.10918873847798949 1107 26-27 0.12360805468330938 1107 28-29 0.16197208211521286 1107 30-31 0.24930103658979164 1107 32-33 0.18336152106809323 1107 34-35 0.15274341289290305 1107 36-37 0.18294960718132103 1107 38-39 0.15726438932528453 1107 40-41 0.1372776034534482 1107 42-43 0.10569026194173148 1107 44-45 0.11163492922039353 1107 46-47 0.14692822791857907 1107 48-49 0.13651930852162053 1107 50-51 0.07950815044433313 1107 52-53 0.12884743555972022 1107 54-55 0.25289132618810584 1107 56-57 0.23935595487107975 1107 58-59 0.304417491343429 1107 60-61 0.20307456703604743 1107 62-63 0.25250060595880086 1107 64-65 0.3311479555617467 1107 66-67 0.2153322221538403 1107 68-69 0.22222905006442772 1107 70-71 0.23398105610512943 1107 72-73 0.3161332599928315 1107 74-75 0.29180977570919 1107 76-77 0.35401528530370285 1107 78-79 0.3106311253913887 1107 80-81 0.3867873958197521 1107 82-83 0.4573698871906231 1107 84-85 0.399508159229061 1107 86-87 0.27665319796543386 1107 88-89 0.3208417650344728 1107 90-91 0.4364470990263065 1107 92-93 0.5305392589632838 1107 94-95 0.48535086491794033 1107 96-97 0.6123861522569101 1107 98-99 0.6409235017611046 1107 100-101 0.8863577439318604 1108 1 0.2696241537840933 1108 2 0.29862112023435117 1108 3 0.3138118778706058 1108 4 0.18174884111842715 1108 5 0.2292950861759664 1108 6 0.24484787545605258 1108 7 0.24104555112612758 1108 8 0.25416651509246435 1108 9 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-1.7612123119656857 2316 66-67 -1.805696142257652 2316 68-69 -1.9700364195830247 2316 70-71 -1.80055716550671 2316 72-73 -1.7127942416320892 2316 74-75 -1.7455135890676061 2316 76-77 -1.9007222664296144 2316 78-79 -1.7651869499343675 2316 80-81 -1.8851535531546268 2316 82-83 -1.896826061740228 2316 84-85 -1.382058555311307 2316 86-87 -1.3600720657216314 2316 88-89 -2.0113086137217167 2316 90-91 -1.732098671968064 2316 92-93 -1.6240978072065246 2316 94-95 -1.553865500052293 2316 96-97 -1.5843869958579404 2316 98-99 -1.416926059861396 2316 100-101 -1.5349057886855384 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 23863.0 3 6820.0 4 5567.0 5 4492.0 6 3974.0 7 3978.0 8 4145.0 9 4967.0 10 5674.0 11 6396.0 12 7064.0 13 7839.0 14 8706.0 15 9859.0 16 11494.0 17 14235.0 18 13380.0 19 11355.0 20 11743.0 21 12577.0 22 13333.0 23 14826.0 24 17219.0 25 19983.0 26 23358.0 27 27984.0 28 33858.0 29 41800.0 30 51370.0 31 65017.0 32 84861.0 33 113630.0 34 166905.0 35 264479.0 36 501591.0 37 1048766.0 38 1165023.0 39 167995.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.254983220691976 18.03746232750919 13.647187199867176 25.060367251931666 2 15.858147415793503 19.668693205987537 38.36838868742421 26.104770690794748 3 18.75784377390327 23.17588732321112 28.98381197597214 29.08245692691347 4 13.614694773638526 16.13146981492757 35.44676557700266 34.807069834431246 5 15.332580044447045 35.9394827916506 33.77205158178162 14.955885582120743 6 34.222640526222875 34.162828833036855 17.296731511010403 14.317799129729863 7 30.149370548233794 30.63762857532229 20.74143904338835 18.471561833055564 8 27.252774680141822 33.824450697236934 19.947086070041557 18.975688552579687 9 26.79010051963945 15.853591753583727 19.463661871527204 37.89264585524962 10-11 25.732769797943956 25.31541952954197 27.36447431495417 21.587336357559902 12-13 26.251731791790146 22.369429994352494 27.476129373301735 23.90270884055563 14-15 23.549296014160493 23.992799215551134 24.64393078704219 27.813973983246186 16-17 24.12886193267889 27.04173824704214 25.32554742261879 23.503852397660175 18-19 24.151172216057205 26.295697718593615 26.411895156197517 23.141234909151663 20-21 24.4518058564573 25.740284982677814 26.303540538519577 23.50436862234531 22-23 24.51404681747278 25.335004510577914 26.148859325241553 24.002089346707752 24-25 24.39851076358372 25.568221455156003 26.060637126143778 23.9726306551165 26-27 24.142539755173893 25.87407621340955 25.995739110539322 23.987644920877234 28-29 24.137727540344407 25.8905850906148 26.049858486856476 23.92182888218432 30-31 24.24287775468919 25.64344638405237 25.990700384337433 24.122975476921 32-33 24.08967494258772 25.64260114952101 26.00421786131474 24.263506046576534 34-35 24.494025010363153 25.645619622650003 25.776029640800367 24.084325726186478 36-37 24.258375676470138 25.54624303149644 25.850183378217746 24.345197913815674 38-39 24.162220851233794 25.724335065404276 26.07141182846309 24.042032254898835 40-41 24.376324633109526 25.429117681364954 26.099083712999505 24.095473972526015 42-43 24.34478570881162 25.438273303512727 25.895108954321312 24.32183203335434 44-45 24.316276246000527 25.612535868992286 25.746322584387777 24.32486530061941 46-47 24.293164970490956 25.638770892577178 25.645434164683834 24.422629972248032 48-49 24.418128240773676 25.714218914234465 25.584003466744047 24.28364937824781 50-51 24.11797783993083 25.75615472742975 25.80041085690368 24.32545657573574 52-53 24.510659143100092 25.645057980232195 25.51311508388745 24.331167792780263 54-55 24.38951601741788 25.40408771750623 25.75804384074962 24.448352424326266 56-57 24.279245055618393 25.792449430976195 25.818816799051525 24.109488714353887 58-59 24.424093457289597 25.494241434665415 25.692603131579343 24.389061976465644 60-61 24.407225449171456 25.4098154681781 25.97491393378218 24.20804514886827 62-63 24.440367876309704 25.519424876333957 25.823867252072187 24.21633999528415 64-65 24.31953574844723 25.467887738197646 25.772609703470543 24.439966809884574 66-67 24.21189874528637 25.679379742931413 25.661452226356275 24.44726928542594 68-69 24.078445022134723 25.44195994283442 25.981009734427086 24.49858530060377 70-71 24.070113076410603 25.52475647158385 25.858201340831748 24.5469291111738 72-73 24.25595593454245 25.44837435734644 25.754177490343867 24.54149221776724 74-75 24.06998294703026 25.577303920232414 25.757261641671565 24.59545149106576 76-77 24.24426588195543 25.453307918253078 25.81638942545587 24.48603677433562 78-79 24.0772498060803 25.538799193214036 25.828153389356245 24.555797611349416 80-81 24.014478207424787 25.58555949962643 25.93892557908329 24.461036713865493 82-83 24.127411677916133 25.54371789539429 25.757530865864396 24.571339560825184 84-85 24.05663355823659 25.40025139850264 25.76742124978791 24.77569379347287 86-87 24.154747066096743 25.35556269557792 26.006791078083648 24.48289916024169 88-89 24.005189375833268 25.313698794392863 25.93217526796792 24.748936561805948 90-91 23.912212332731965 25.463186966376984 26.017385998932284 24.607214701958767 92-93 24.080830862374604 25.32208746993834 26.05759784796501 24.53948381972205 94-95 23.932172621668574 25.2453160901136 26.00765857544292 24.8148527127749 96-97 24.099402366661053 25.196890194664366 25.909693631275022 24.794013807399562 98-99 23.86057512373686 25.054425706846207 25.98615478065602 25.09884438876091 100-101 23.737378160057364 25.0400816444583 26.301902852301893 24.92063734318244 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 213.0 1 159.0 2 128.5 3 143.5 4 123.0 5 140.5 6 238.0 7 423.5 8 632.5 9 792.5 10 827.0 11 783.5 12 828.5 13 928.5 14 978.0 15 988.5 16 1063.5 17 1162.0 18 1234.0 19 1348.0 20 1462.5 21 1667.0 22 1950.0 23 2359.5 24 2915.5 25 3675.5 26 4868.0 27 6722.5 28 9250.5 29 12572.5 30 17242.0 31 22757.0 32 28736.0 33 35627.5 34 43387.0 35 51360.5 36 59799.0 37 68546.0 38 78416.5 39 85600.0 40 90916.0 41 101520.5 42 113569.0 43 125678.5 44 138231.0 45 147333.0 46 154714.5 47 163787.5 48 175745.0 49 183883.0 50 186727.5 51 187065.0 52 182435.0 53 179831.0 54 178871.0 55 171336.5 56 160364.5 57 147485.0 58 132215.5 59 115760.0 60 96830.5 61 77015.5 62 60525.5 63 46686.5 64 35083.5 65 26320.0 66 19032.0 67 14126.5 68 10021.5 69 6690.0 70 4930.5 71 3633.5 72 2700.5 73 2002.0 74 1477.5 75 1124.0 76 759.5 77 486.5 78 302.5 79 203.5 80 141.0 81 104.0 82 87.5 83 64.0 84 53.0 85 50.5 86 42.0 87 34.0 88 23.5 89 18.5 90 20.0 91 19.5 92 22.0 93 21.0 94 17.5 95 18.0 96 13.0 97 9.5 98 8.5 99 4.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02167425223829778 2 0.03417382100317539 3 0.023674183240678198 4 0.022324229814071415 5 0.041798557949750736 6 0.022999206527374808 7 0.02557411769293959 8 0.039223646784185946 9 0.025474121142820574 10-11 0.027474052145200992 12-13 0.025711612949353247 14-15 0.035173786504365596 16-17 0.04297351741364923 18-19 0.0442734725651965 20-21 0.03772369853240063 22-23 0.043973482914839435 24-25 0.019324333310500787 26-27 0.024324160816451834 28-29 0.025986603462180558 30-31 0.026199096131183475 32-33 0.03572376753002021 34-35 0.03988612392872446 36-37 0.029061497378340448 38-39 0.01476199071132046 40-41 0.017036912226528186 42-43 0.019461828566914443 44-45 0.021549256550649 46-47 0.015036981224147766 48-49 0.02491164054840108 50-51 0.017299403170590616 52-53 0.026486586212775658 54-55 0.022836712133431396 56-57 0.021911744044830456 58-59 0.021949242751125086 60-61 0.018661856165962276 62-63 0.021474259138059736 64-65 0.018861849266200315 66-67 0.017486896702063777 68-69 0.021961742319889965 70-71 0.03564877011743095 72-73 0.02491164054840108 74-75 0.035523774429782176 76-77 0.043111012670062884 78-79 0.026499085781540536 80-81 0.022149235851363126 82-83 0.015361970012034586 84-85 0.03268637232015495 86-87 0.019324333310500787 88-89 0.029961466329411636 90-91 0.023574186690559176 92-93 0.04224854242528633 94-95 0.07624736946575343 96-97 0.09687165792780149 98-99 0.07364745916265888 100-101 0.08239715729807322 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4000138.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.12598261256702 #Duplication Level Percentage of deduplicated Percentage of total 1 77.84209575309006 40.5759572975135 2 11.170448429109008 11.645412011806258 3 4.000074759857453 6.2552348214389335 4 1.98173152200447 4.131988114351239 5 1.163545783307371 3.032548363480285 6 0.7782570474270765 2.4340447993374923 7 0.5388122908727729 1.9660284073830094 8 0.38901496096317845 1.6222229672956063 9 0.29360166894222395 1.377384794127272 >10 1.6713807972799606 15.55874608027924 >50 0.10899203969634466 3.919309419174409 >100 0.05771758907562165 5.477404122702431 >500 0.003125314429045319 1.1006455883728472 >1k 0.0012020439454200826 0.9030732127375432 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.4999137529755222E-5 2 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 3 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 4 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 5 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 6 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 7 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 8 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 9 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 10-11 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 12-13 2.4999137529755222E-5 0.0 0.0 0.0 2.4999137529755222E-5 14-15 2.4999137529755222E-5 0.0 0.0 0.0 6.249784382438806E-5 16-17 2.4999137529755222E-5 0.0 0.0 2.4999137529755222E-5 9.999655011902089E-5 18-19 3.749870629463283E-5 0.0 0.0 2.4999137529755222E-5 9.999655011902089E-5 20-21 4.9998275059510445E-5 0.0 0.0 4.9998275059510445E-5 9.999655011902089E-5 22-23 4.9998275059510445E-5 0.0 0.0 4.9998275059510445E-5 9.999655011902089E-5 24-25 7.499741258926566E-5 0.0 0.0 1.3749525641365372E-4 9.999655011902089E-5 26-27 7.499741258926566E-5 0.0 0.0 2.3749180653267462E-4 9.999655011902089E-5 28-29 8.749698135414327E-5 0.0 0.0 8.124719697170448E-4 9.999655011902089E-5 30-31 9.999655011902089E-5 0.0 0.0 0.002637409009389176 9.999655011902089E-5 32-33 1.1249611888389851E-4 0.0 0.0 0.0074872416901616895 9.999655011902089E-5 34-35 1.3749525641365372E-4 0.0 0.0 0.013749525641365373 1.2499568764877612E-4 36-37 1.4999482517853133E-4 1.2499568764877611E-5 0.0 0.022936708683550418 1.2499568764877612E-4 38-39 1.7499396270828657E-4 2.4999137529755222E-5 0.0 0.039561135140837644 1.2499568764877612E-4 40-41 1.7499396270828657E-4 2.4999137529755222E-5 0.0 0.05776050726249944 1.2499568764877612E-4 42-43 1.7499396270828657E-4 2.4999137529755222E-5 0.0 0.07748482677347632 1.2499568764877612E-4 44-45 1.7499396270828657E-4 2.4999137529755222E-5 0.0 0.10232146990928813 1.4999482517853133E-4 46-47 2.6249094406242984E-4 2.4999137529755222E-5 0.0 0.13189544960698857 1.7499396270828657E-4 48-49 2.9998965035706265E-4 2.4999137529755222E-5 0.0 0.16186941550516507 1.7499396270828657E-4 50-51 2.9998965035706265E-4 2.4999137529755222E-5 0.0 0.1947932796318527 1.7499396270828657E-4 52-53 2.9998965035706265E-4 2.4999137529755222E-5 0.0 0.22836712133431397 2.124926690029194E-4 54-55 2.9998965035706265E-4 4.9998275059510445E-5 0.0 0.26901571895769594 2.2499223776779702E-4 56-57 2.9998965035706265E-4 4.9998275059510445E-5 0.0 0.31968897073050984 2.2499223776779702E-4 58-59 2.9998965035706265E-4 4.9998275059510445E-5 0.0 0.3745620776083225 2.2499223776779702E-4 60-61 2.9998965035706265E-4 4.9998275059510445E-5 0.0 0.4335225434722502 2.2499223776779702E-4 62-63 2.9998965035706265E-4 4.9998275059510445E-5 0.0 0.49867029587479234 2.4999137529755223E-4 64-65 3.249887878868179E-4 4.9998275059510445E-5 0.0 0.5729177343381653 2.4999137529755223E-4 66-67 3.4998792541657313E-4 4.9998275059510445E-5 0.0 0.6623771479883944 2.4999137529755223E-4 68-69 3.4998792541657313E-4 4.9998275059510445E-5 0.0 0.7640986385969684 2.4999137529755223E-4 70-71 3.4998792541657313E-4 4.9998275059510445E-5 0.0 0.8747198221661353 2.7499051282730744E-4 72-73 3.4998792541657313E-4 4.9998275059510445E-5 0.0 0.9964156236609838 3.124892191219403E-4 74-75 3.6248749418145074E-4 4.9998275059510445E-5 0.0 1.1367482821842647 3.249887878868179E-4 76-77 3.7498706294632835E-4 4.9998275059510445E-5 0.0 1.3004426347291018 3.249887878868179E-4 78-79 3.7498706294632835E-4 4.9998275059510445E-5 0.0 1.4834113223093803 3.249887878868179E-4 80-81 4.4998447553559404E-4 4.9998275059510445E-5 0.0 1.685566847943746 3.249887878868179E-4 82-83 4.4998447553559404E-4 4.9998275059510445E-5 0.0 1.904109308228866 3.249887878868179E-4 84-85 4.4998447553559404E-4 4.9998275059510445E-5 0.0 2.144626010402641 3.249887878868179E-4 86-87 4.4998447553559404E-4 4.9998275059510445E-5 0.0 2.4126667629966767 3.4998792541657313E-4 88-89 4.4998447553559404E-4 4.9998275059510445E-5 0.0 2.715081329694126 3.9998620047608356E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5300 0.0 38.445095 1 GGTATCA 3315 0.0 37.25198 1 ATCAACG 5755 0.0 34.086353 3 TCAACGC 5790 0.0 33.715813 4 CAACGCA 6000 0.0 32.536167 5 TATCAAC 6375 0.0 30.846954 2 AACGCAG 6380 0.0 30.523064 6 GTACATG 9970 0.0 28.011843 1 ACGCAGA 7510 0.0 25.993626 7 TACATGG 10565 0.0 25.626982 2 ACATGGG 10945 0.0 24.215546 3 CGCAGAG 8245 0.0 23.618818 8 CATGGGG 8900 0.0 21.934221 4 GCAGAGT 10110 0.0 18.983992 9 TATAAGC 2210 0.0 18.268482 3 CCTATAA 2235 0.0 17.850946 1 CTATAAG 2170 0.0 17.730354 2 ATGGGGA 5350 0.0 17.401157 5 AGAGTAC 9080 0.0 16.926512 10-11 CAGAGTA 9195 0.0 16.430637 10-11 >>END_MODULE