FastQCFastQC Report
Fri 27 May 2016
SRR940228_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940228_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4000138
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33350.043.1833271
GTATCAA56700.039.9018671
ATCAACG63900.034.1935273
TCAACGC64200.034.1073044
CAACGCA64650.033.8694765
TATCAAC67600.032.8142552
AACGCAG69250.031.4824836
ACGCAGA80250.027.1667887
CGCAGAG86800.025.0620388
GTACATG102450.025.006111
TACATGG105150.023.716052
ACATGGG108250.023.1682363
CATGGGG93150.020.6515964
GCAGAGT108950.019.7924379
TAGGACG6850.018.0286944
CAGAGTA102450.017.22334510-11
AGAGTAC98550.017.13379910-11
GTCTAGG15350.016.4110681
TATAAGC18750.016.2130833
ATGGGGA58950.015.8729815
Produced by FastQC (version 0.11.5)