FastQCFastQC Report
Fri 27 May 2016
SRR940227_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940227_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5648578
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46150.040.3617171
GTATCAA81150.038.7636531
TCAACGC91150.035.342214
ATCAACG91400.035.039463
CAACGCA94650.033.881415
AACGCAG99850.032.247396
TATCAAC101150.030.5828552
ACGCAGA117950.027.2588487
GTACATG161000.026.297051
CGCAGAG124600.025.689458
TACATGG168950.024.2163072
ACATGGG172350.023.9580923
CATGGGG148200.021.0638524
GCAGAGT151800.020.898999
AGAGTAC136600.018.3595310-11
GCTTATA27150.017.3268891
CAGAGTA143550.017.02395610-11
ATGGGGA91450.016.6243485
TTATAGG28450.016.5347123
CCTATAA29500.016.4298421
Produced by FastQC (version 0.11.5)