FastQCFastQC Report
Fri 27 May 2016
SRR940227_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940227_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5648578
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46250.041.4036031
GTATCAA84900.040.968321
TCAACGC97500.034.495464
ATCAACG98900.034.007153
CAACGCA102650.032.7179455
AACGCAG107700.031.44546
TATCAAC108400.031.2459342
ACGCAGA124050.027.1472027
GTACATG159700.025.4988561
CGCAGAG132650.025.4229958
TACATGG168600.023.3013552
ACATGGG168050.022.7557243
GCAGAGT163050.020.6829819
CATGGGG142500.020.1351744
GCTTATA29500.019.1676881
AGAGTAC144100.018.90362210-11
TTATAGG28100.018.5960043
TATAAGC28500.018.1683253
CCTATAA29100.018.1248651
CAGAGTA148150.017.29678510-11
Produced by FastQC (version 0.11.5)