##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940227_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5648578 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.04690454836598 34.0 33.0 34.0 31.0 34.0 2 33.10695576833674 34.0 33.0 34.0 31.0 34.0 3 33.16527274652134 34.0 33.0 34.0 31.0 34.0 4 36.47901471839461 37.0 37.0 37.0 35.0 37.0 5 36.45730606889026 37.0 37.0 37.0 35.0 37.0 6 36.48528957199493 37.0 37.0 37.0 35.0 37.0 7 36.461898906238 37.0 37.0 37.0 35.0 37.0 8 36.45850973466242 37.0 37.0 37.0 35.0 37.0 9 38.309324753946925 39.0 39.0 39.0 37.0 39.0 10-11 38.26718450201095 39.0 39.0 39.0 37.0 39.0 12-13 38.22641548014386 39.0 39.0 39.0 37.0 39.0 14-15 39.75467551302292 41.0 40.0 41.0 37.0 41.0 16-17 39.73520671928404 41.0 40.0 41.0 37.0 41.0 18-19 39.69506484640913 41.0 40.0 41.0 37.0 41.0 20-21 39.634108265832566 41.0 40.0 41.0 37.0 41.0 22-23 39.56442054973836 41.0 40.0 41.0 37.0 41.0 24-25 39.49152494663259 41.0 39.0 41.0 37.0 41.0 26-27 39.32341617660232 41.0 39.0 41.0 36.0 41.0 28-29 39.46073949939259 41.0 39.0 41.0 37.0 41.0 30-31 39.36616224472779 41.0 39.0 41.0 36.0 41.0 32-33 39.25497957184976 41.0 39.0 41.0 36.0 41.0 34-35 39.394370760216106 41.0 39.5 41.0 36.0 41.0 36-37 39.372736111637295 41.0 39.5 41.0 36.0 41.0 38-39 39.292380046801156 41.0 39.0 41.0 36.0 41.0 40-41 39.17790344755795 41.0 39.0 41.0 35.0 41.0 42-43 39.06920237270336 41.0 39.0 41.0 35.0 41.0 44-45 38.94788396300804 41.0 39.0 41.0 35.0 41.0 46-47 38.786628510750845 41.0 38.5 41.0 35.0 41.0 48-49 38.62860033091515 40.0 38.0 41.0 35.0 41.0 50-51 38.445778477344206 40.0 38.0 41.0 35.0 41.0 52-53 38.24950766369872 40.0 38.0 41.0 34.0 41.0 54-55 38.04540452482023 40.0 37.0 41.0 34.0 41.0 56-57 37.82570808086567 40.0 37.0 41.0 34.0 41.0 58-59 37.527768935827744 39.5 36.0 41.0 33.5 41.0 60-61 37.1971399704492 39.0 35.5 41.0 33.0 41.0 62-63 36.87169540723347 39.0 35.0 40.5 33.0 41.0 64-65 36.504872199693445 38.0 35.0 40.0 32.0 41.0 66-67 36.0985466607702 37.0 35.0 40.0 32.0 41.0 68-69 35.668600398188715 36.5 35.0 39.0 31.0 41.0 70-71 35.26519143402109 36.0 35.0 39.0 31.0 40.5 72-73 34.83405717332752 35.5 34.5 38.0 31.0 40.0 74-75 34.41666592901789 35.0 34.0 37.0 30.0 39.0 76-77 33.09576888200888 34.5 32.5 36.0 28.5 38.5 78-79 33.72778246135576 35.0 34.0 36.0 30.0 38.5 80-81 33.56708803525419 35.0 34.0 36.0 30.0 37.0 82-83 33.32910690088727 35.0 34.0 35.5 30.0 37.0 84-85 33.08100330738108 35.0 34.0 35.0 29.0 36.5 86-87 32.83276082936271 35.0 34.0 35.0 29.0 36.0 88-89 32.60096417186768 35.0 33.0 35.0 29.0 36.0 90-91 32.381741829536566 35.0 33.0 35.0 29.0 35.5 92-93 32.15673475696007 35.0 33.0 35.0 27.5 35.0 94-95 31.923555273557344 35.0 33.0 35.0 27.0 35.0 96-97 31.611703069338866 35.0 33.0 35.0 26.0 35.0 98-99 31.212215800153594 34.0 32.0 35.0 25.0 35.0 100-101 28.7534691031973 32.0 28.5 34.5 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.08966261678942544 1101 2 0.08343856315547526 1101 3 0.09795421425645401 1101 4 0.03869670343527076 1101 5 0.058553216022325216 1101 6 0.07981800736124 1101 7 0.06202785087887008 1101 8 0.04815272378690594 1101 9 0.0811656371057694 1101 10-11 0.08019977766163322 1101 12-13 0.10947006108435886 1101 14-15 0.15615906154902603 1101 16-17 0.1499804338356725 1101 18-19 0.09578394441722793 1101 20-21 0.11579280066239761 1101 22-23 0.1385824098427122 1101 24-25 0.15531115390589179 1101 26-27 0.1586595992523243 1101 28-29 0.11687725203031363 1101 30-31 0.06709975999514484 1101 32-33 0.16566016351074353 1101 34-35 0.18292927955400273 1101 36-37 0.1634051835702408 1101 38-39 0.2110753796375846 1101 40-41 0.20883343675343724 1101 42-43 0.17296165092594862 1101 44-45 0.2100093026106009 1101 46-47 0.18574054492189873 1101 48-49 0.20499575592074848 1101 50-51 0.1425205870274766 1101 52-53 0.24443033679457926 1101 54-55 0.21932077099079805 1101 56-57 0.21447570548597383 1101 58-59 0.2193459910075788 1101 60-61 0.3158728652868277 1101 62-63 0.2940545666763654 1101 64-65 0.25778052048708133 1101 66-67 0.29576339824631503 1101 68-69 0.29239856818904997 1101 70-71 0.24550919971810714 1101 72-73 0.2899921060990991 1101 74-75 0.35321789559048256 1101 76-77 0.15092490228037292 1101 78-79 0.28312902055956357 1101 80-81 0.2581260484272079 1101 82-83 0.30554304067938176 1101 84-85 0.32068247969753116 1101 86-87 0.39074520040643534 1101 88-89 0.3727996990232114 1101 90-91 0.417169416055458 1101 92-93 0.36412004390694364 1101 94-95 0.29984383519330393 1101 96-97 0.30401898436819863 1101 98-99 0.3193467466719362 1101 100-101 0.5519793620447722 1102 1 0.08804323479953524 1102 2 0.07439642120340295 1102 3 0.07831255594103936 1102 4 0.05726424978378475 1102 5 0.05808660618658479 1102 6 0.07950549964304088 1102 7 0.04397915490910265 1102 8 0.058812226962878356 1102 9 0.03127576885223249 1102 10-11 0.07630312337652612 1102 12-13 0.058131322326737234 1102 14-15 0.10786728239929033 1102 16-17 0.12225017316435327 1102 18-19 0.09795202050580798 1102 20-21 0.11418597361676319 1102 22-23 0.09738739245289452 1102 24-25 0.0927337379865989 1102 26-27 0.12376192485461246 1102 28-29 0.06535576492617423 1102 30-31 0.11068139557320222 1102 32-33 0.16891608529062552 1102 34-35 0.16915742253741683 1102 36-37 0.17668404852843622 1102 38-39 0.16768685818714602 1102 40-41 0.20801126565742578 1102 42-43 0.16832157928605085 1102 44-45 0.15548173898828566 1102 46-47 0.1969601557822429 1102 48-49 0.21486306269622446 1102 50-51 0.20196347681265792 1102 52-53 0.2487227431302017 1102 54-55 0.173463285138034 1102 56-57 0.22401832381492426 1102 58-59 0.28348033227902647 1102 60-61 0.26848234776865354 1102 62-63 0.2532721410536496 1102 64-65 0.26117351755996765 1102 66-67 0.18659329315899953 1102 68-69 0.1929973510891685 1102 70-71 0.24262509888038863 1102 72-73 0.3095923948441026 1102 74-75 0.3181872061425395 1102 76-77 0.2156434694525089 1102 78-79 0.363123175306832 1102 80-81 0.365256152825971 1102 82-83 0.3303069104312826 1102 84-85 0.31066999585429755 1102 86-87 0.30898154695133684 1102 88-89 0.26915601470278006 1102 90-91 0.2880411969565202 1102 92-93 0.30235164539393367 1102 94-95 0.20739077870990386 1102 96-97 0.15275512621698795 1102 98-99 0.22398556468848696 1102 100-101 0.4456681543526848 1103 1 -0.001623249935470028 1103 2 -0.0034249921241524817 1103 3 0.008789924890038492 1103 4 -0.01829237550285967 1103 5 0.020792719235494417 1103 6 0.02572024748198487 1103 7 -0.047868261008439106 1103 8 -0.030542696174620687 1103 9 -0.030624375329338704 1103 10-11 -0.0172018800343281 1103 12-13 0.02111791335394031 1103 14-15 -0.04116478022699255 1103 16-17 -0.055699448304629584 1103 18-19 -0.012015622063188403 1103 20-21 -0.0767094130663537 1103 22-23 0.012246095097346199 1103 24-25 -0.024350348680194145 1103 26-27 0.031016348303886332 1103 28-29 0.01653119724600316 1103 30-31 0.01119052857163183 1103 32-33 -0.023219309296514723 1103 34-35 -0.016567673789161574 1103 36-37 -0.04048410536427838 1103 38-39 -0.08134396399598387 1103 40-41 -0.07217883551921034 1103 42-43 -0.11882182232533012 1103 44-45 -0.09358930692145861 1103 46-47 -0.047725851061521496 1103 48-49 -0.01918929967334293 1103 50-51 -0.13241211892648153 1103 52-53 -0.10546505096726122 1103 54-55 -0.1069500232645666 1103 56-57 -0.10842972483018087 1103 58-59 -0.1197973465051021 1103 60-61 -0.08733398133788484 1103 62-63 -0.18595231067772744 1103 64-65 -0.10777137890872979 1103 66-67 -0.14046016979555986 1103 68-69 -0.1654397224631552 1103 70-71 -0.3910981180660045 1103 72-73 -0.27454965664285425 1103 74-75 -0.21412321859970262 1103 76-77 -0.27946131389938245 1103 78-79 -0.13084261283592724 1103 80-81 -0.08775966913518829 1103 82-83 -0.197591852868662 1103 84-85 -0.17681755981693215 1103 86-87 -0.06058640017183592 1103 88-89 -0.21949781141871227 1103 90-91 -0.11471647500299298 1103 92-93 -0.11133327885489308 1103 94-95 -0.12191086050307476 1103 96-97 -0.20711146738685215 1103 98-99 -0.23644081696931707 1103 100-101 -0.14584807775264963 1104 1 0.005134272601679868 1104 2 0.011792641856729347 1104 3 0.0015649999428504202 1104 4 0.004570078662304411 1104 5 -0.011905424022280897 1104 6 -0.04037522531877613 1104 7 -0.008488010187605255 1104 8 0.004662036306740447 1104 9 -0.03479946759551211 1104 10-11 -0.016489059403518525 1104 12-13 -0.04560782837577193 1104 14-15 0.01588473034261284 1104 16-17 -0.07734205461205335 1104 18-19 0.011198342574431308 1104 20-21 0.03468542735640767 1104 22-23 0.02245342662583738 1104 24-25 0.011639956447943689 1104 26-27 0.005954184209421953 1104 28-29 8.389176089806938E-4 1104 30-31 -0.012160312113593363 1104 32-33 0.028403838243299617 1104 34-35 0.02664542591966068 1104 36-37 0.009112104969361212 1104 38-39 0.024336168291263505 1104 40-41 0.02303959947947476 1104 42-43 0.06797616865384981 1104 44-45 0.05619740078458335 1104 46-47 -0.11809196306597158 1104 48-49 0.0415903761189611 1104 50-51 0.10497515273019076 1104 52-53 0.055745427538617776 1104 54-55 0.05917457164624551 1104 56-57 0.10981332783162401 1104 58-59 0.10973888549010269 1104 60-61 0.12966642854463117 1104 62-63 0.0669293295681328 1104 64-65 -0.03458966801991181 1104 66-67 -0.0874342824339962 1104 68-69 0.00791586893936369 1104 70-71 -0.030312775498821054 1104 72-73 -0.015015520928280068 1104 74-75 0.03240556222564095 1104 76-77 -0.11413635205424555 1104 78-79 0.12929186015826843 1104 80-81 0.16733755227039637 1104 82-83 0.05133284464060495 1104 84-85 0.004025630722189533 1104 86-87 -0.0012956375261907738 1104 88-89 -0.04684396274119962 1104 90-91 -0.034329118983812634 1104 92-93 -0.0026443234417641293 1104 94-95 -0.06451356760239335 1104 96-97 -0.09655886735975372 1104 98-99 -0.09782287519308852 1104 100-101 -0.10665189059739788 1105 1 -0.03724585091419641 1105 2 -0.04414267874962263 1105 3 -0.03214088497550449 1105 4 -0.026254100286116966 1105 5 -0.017814853438977707 1105 6 -0.08160004420465583 1105 7 -0.04373936174634707 1105 8 -0.05386902787635961 1105 9 -0.04171100750299672 1105 10-11 0.03593418205196741 1105 12-13 0.014362363633075859 1105 14-15 -0.03302933022952459 1105 16-17 -0.06870204317355189 1105 18-19 -0.11129175777186617 1105 20-21 -0.13078798117730628 1105 22-23 -0.08246558197898679 1105 24-25 -0.03347068008336862 1105 26-27 0.06282433409678134 1105 28-29 -0.028281149056695654 1105 30-31 -0.028524689480171617 1105 32-33 -0.06606788211860248 1105 34-35 -0.06742557445738839 1105 36-37 -0.0343942714951595 1105 38-39 -0.09060132297943113 1105 40-41 -0.0813201815108684 1105 42-43 -0.21843062554763293 1105 44-45 -0.17990844284035745 1105 46-47 -0.1222061917928201 1105 48-49 -0.156201889315966 1105 50-51 -0.23957551091786655 1105 52-53 -0.06781102822802154 1105 54-55 -0.05929956504267864 1105 56-57 -0.04954480528662231 1105 58-59 -0.13724201685926118 1105 60-61 -0.2488809818671669 1105 62-63 -0.07342382846694306 1105 64-65 -0.1942306582447486 1105 66-67 -0.18350874472257317 1105 68-69 -0.25463245622085395 1105 70-71 -0.22105886960031995 1105 72-73 -0.26845348723881557 1105 74-75 -0.22986425322924475 1105 76-77 -0.48460932722796457 1105 78-79 -0.3054907725395992 1105 80-81 -0.28680067687160005 1105 82-83 -0.28448464897078907 1105 84-85 -0.3133443212548954 1105 86-87 -0.32708174428069725 1105 88-89 -0.3400703803666474 1105 90-91 -0.2934864021644188 1105 92-93 -0.38991876232336864 1105 94-95 -0.42661965854616213 1105 96-97 -0.5115417096161643 1105 98-99 -0.6127092741481661 1105 100-101 -0.4515207426129635 1106 1 -0.06978540464920258 1106 2 -0.037994623795036375 1106 3 -0.03041507790614162 1106 4 -0.022087513723185737 1106 5 -0.0044318662208553405 1106 6 -0.021390536047562136 1106 7 -0.021569143615302266 1106 8 0.0227938623648285 1106 9 -0.07059496200169235 1106 10-11 -0.02692390959534663 1106 12-13 -0.04109837145247042 1106 14-15 -0.009067135616639632 1106 16-17 0.027481941473944005 1106 18-19 -0.01092142143006214 1106 20-21 -0.02610921877185035 1106 22-23 -0.229841107850703 1106 24-25 -0.0626927468670857 1106 26-27 0.013693172759509764 1106 28-29 -0.0923786876886794 1106 30-31 0.009383826538112316 1106 32-33 0.050665803432721646 1106 34-35 -0.010443520377009463 1106 36-37 -0.15855851008715405 1106 38-39 -0.12763849662749038 1106 40-41 -0.07181151398847163 1106 42-43 -0.044942920763354266 1106 44-45 -0.14786743240958344 1106 46-47 -0.035308577483299075 1106 48-49 0.0023221579748948784 1106 50-51 -0.05907749965147957 1106 52-53 -0.0955917679811904 1106 54-55 -0.13343432545948275 1106 56-57 -0.05978081474051322 1106 58-59 -0.2391969727768668 1106 60-61 -0.23073476564727713 1106 62-63 -0.22146080628930775 1106 64-65 -0.02671466842112835 1106 66-67 -0.16504552262091465 1106 68-69 -0.08516314608615261 1106 70-71 -0.08778916471440112 1106 72-73 0.014893325876855101 1106 74-75 -0.0011729463550338437 1106 76-77 -0.24497817848374126 1106 78-79 -0.127740077276421 1106 80-81 -0.03078572452177042 1106 82-83 -0.011101958322775829 1106 84-85 -0.06121393442762013 1106 86-87 0.029121373610088597 1106 88-89 0.01174728147159243 1106 90-91 0.07206333152604572 1106 92-93 -0.06264382364557974 1106 94-95 -0.07855996106854235 1106 96-97 -0.07669311536595558 1106 98-99 -0.19861233536277112 1106 100-101 -0.17878417243292333 1107 1 0.049970170970155436 1107 2 0.05005370221181238 1107 3 0.018685110529929716 1107 4 0.006495172783871794 1107 5 0.06563401347533215 1107 6 0.06353620618563127 1107 7 0.013523160530539258 1107 8 0.07988368486068254 1107 9 -0.016684442274602418 1107 10-11 0.04749664149743893 1107 12-13 0.060780924343774245 1107 14-15 0.07504344757466441 1107 16-17 0.15228154805985383 1107 18-19 0.1645998638056554 1107 20-21 0.13612711481086137 1107 22-23 0.17018796953828286 1107 24-25 0.1404572828550883 1107 26-27 0.1116769699970348 1107 28-29 0.10196508202160004 1107 30-31 0.13581521759613935 1107 32-33 0.14214705338603295 1107 34-35 0.044336541792311834 1107 36-37 0.0659930604564849 1107 38-39 0.12640832530593826 1107 40-41 0.11117059614684166 1107 42-43 0.17068941973211338 1107 44-45 0.12578361508789726 1107 46-47 0.09242013470186805 1107 48-49 0.06961114479931041 1107 50-51 0.00951679024123564 1107 52-53 0.19715608485440583 1107 54-55 0.22753391112828325 1107 56-57 0.18868000464510715 1107 58-59 0.1425145724095529 1107 60-61 0.10955317298595446 1107 62-63 0.1214673761617675 1107 64-65 0.04936276912585669 1107 66-67 0.03343673906010736 1107 68-69 4.022932778724453E-5 1107 70-71 -0.046849032563706317 1107 72-73 -7.114802566903222E-4 1107 74-75 -0.22620374464164428 1107 76-77 -0.2605893657234475 1107 78-79 -0.013354369246663111 1107 80-81 0.030907810381584966 1107 82-83 0.10082351089085506 1107 84-85 0.03952374367862177 1107 86-87 0.015346699851370715 1107 88-89 0.0010298023455916905 1107 90-91 0.04800238133442747 1107 92-93 0.105622166116909 1107 94-95 0.05963556616290688 1107 96-97 0.08786002666403547 1107 98-99 0.08587536453260824 1107 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-0.47946750062875054 2316 52-53 -0.3192242472040405 2316 54-55 -0.28418433914632857 2316 56-57 -0.1986218636186763 2316 58-59 -0.4740235028513524 2316 60-61 -0.31879927137810427 2316 62-63 -0.2500352353686708 2316 64-65 -0.4536308655137873 2316 66-67 -0.4769994877391568 2316 68-69 -0.3945672642156808 2316 70-71 -0.3006072432242135 2316 72-73 -0.401426820498223 2316 74-75 -0.3594270878417447 2316 76-77 -0.3840098199179991 2316 78-79 -0.5983322854433979 2316 80-81 -0.535850753678595 2316 82-83 -0.5685510317359572 2316 84-85 -0.4779828047535588 2316 86-87 -0.5393425424862031 2316 88-89 -0.4167185993909186 2316 90-91 -0.43858209417586025 2316 92-93 -0.5751839184353109 2316 94-95 -0.5169946001015902 2316 96-97 -0.5774646954319707 2316 98-99 -0.47573378400946 2316 100-101 -0.6526810508594316 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 5.0 4 14.0 5 60.0 6 93.0 7 182.0 8 469.0 9 929.0 10 1503.0 11 2504.0 12 3709.0 13 5116.0 14 6154.0 15 7632.0 16 10409.0 17 15036.0 18 14639.0 19 11061.0 20 10717.0 21 12170.0 22 12486.0 23 13855.0 24 16271.0 25 20396.0 26 24952.0 27 31463.0 28 40406.0 29 50098.0 30 63348.0 31 80782.0 32 104007.0 33 139502.0 34 204141.0 35 329557.0 36 669010.0 37 1598383.0 38 1902620.0 39 244882.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.620665017647944 17.987873709649342 13.595108119684832 24.796353153017876 2 16.097280649916208 19.900072267203825 38.06720106393187 25.9354460189481 3 18.78734478087905 23.772343577914818 28.79667992494353 28.643631716262608 4 13.685623494382776 16.47829282149326 35.64080007350714 34.19528361061682 5 15.341414893971123 36.18788247483572 33.56706451249663 14.903638118696536 6 33.9130063774126 34.525626052375756 17.386809911040558 14.174557659171086 7 30.19212745519109 30.71925463243435 20.796251492863114 18.292366419511442 8 27.40718106397752 33.71214843806707 19.871213604556758 19.009456893398657 9 27.104450004939295 15.790505150145753 19.266300297172137 37.83874454774281 10-11 25.819666471809366 25.375315698924577 27.17707890375241 21.627938925513643 12-13 26.561747045008495 22.37807462338309 27.362922137217545 23.697256194390874 14-15 23.762582370288595 23.78404794976718 24.599686859241388 27.85368282070284 16-17 24.294273709241512 26.90629393804954 25.123827625289053 23.67560472741989 18-19 24.367672713380255 26.104729367285007 26.28634144735188 23.24125647198286 20-21 24.772485773943167 25.495827342001455 26.158514973520113 23.57317191053527 22-23 24.70697039395416 25.215452531753137 26.01384844605083 24.06372862824188 24-25 24.62267240738315 25.47468903468861 25.85276018227719 24.049878375651055 26-27 24.414232569181674 25.758798516752478 25.831604792839368 23.99536412122648 28-29 24.350969798760495 25.7470112204156 25.921118065313483 23.980900915510425 30-31 24.488580330705204 25.464297085693705 25.80521550117459 24.241907082426504 32-33 24.36640418079543 25.500927725077894 25.82078286523958 24.311885228887096 34-35 24.754531941010303 25.55597719802381 25.577681900588388 24.1118089603775 36-37 24.603926518022394 25.416516812348817 25.62812010852713 24.351436561101657 38-39 24.51173551998397 25.582527142229427 25.897756922184662 24.007980415601946 40-41 24.685074770344244 25.32918549372646 25.85343387335859 24.13230586257071 42-43 24.62729557775426 25.335872143396088 25.66553033347508 24.371301945374572 44-45 24.542505202430743 25.526162723022487 25.532615693393794 24.398716381152973 46-47 24.602385567093354 25.49746230863302 25.457822361161703 24.44232976311192 48-49 24.698902896516074 25.608806570228253 25.40643461727075 24.285855915984925 50-51 24.442727001379815 25.610268637522577 25.471109720003867 24.47589464109374 52-53 24.84083813373412 25.440060220514084 25.285676109198192 24.43342553655361 54-55 24.601288587837956 25.295755039073143 25.500782595902745 24.602173777186156 56-57 24.511478818208758 25.714808222529633 25.550873157810695 24.222839801450913 58-59 24.688119792591497 25.376834780810626 25.498042599507166 24.437002827090712 60-61 24.737480226205744 25.314696654881686 25.685347692590405 24.262475426322165 62-63 24.739595251004125 25.46588623211658 25.519997344457106 24.274521172422187 64-65 24.55702116540001 25.33153260780276 25.51668028688861 24.59476593990862 66-67 24.44756775436456 25.629106573243543 25.40111065357119 24.522215018820706 68-69 24.306178377890333 25.443820294339986 25.715304935585465 24.534696392184216 70-71 24.319457038567936 25.398011676567094 25.599354386183563 24.683176898681403 72-73 24.53065282649291 25.44038364984304 25.42187425865064 24.60708926501341 74-75 24.384793135604735 25.476928442476787 25.41745318613967 24.720825235778808 76-77 24.51997602225837 25.352354541997517 25.534967067782794 24.59270236796132 78-79 24.32736168288727 25.455849242057027 25.536072972702158 24.680716102353546 80-81 24.301063028606492 25.524220432115836 25.598309875512033 24.576406663765642 82-83 24.380487246997106 25.465331646057255 25.496127149046337 24.6580539578993 84-85 24.339603802114564 25.38752927525887 25.40256855708403 24.87029836554254 86-87 24.420265723972747 25.237315510526436 25.70861821020226 24.633800555298553 88-89 24.222801974975468 25.261798260145717 25.59721019977334 24.918189565105475 90-91 24.200584792008645 25.464211408896944 25.694148397522596 24.641055401571816 92-93 24.266485963008275 25.27109089653683 25.734886133679396 24.727537006775503 94-95 24.205932891428603 25.178018255213967 25.648154278829118 24.967894574528316 96-97 24.375653654868458 25.10950862857621 25.524625665715533 24.9902120508398 98-99 24.034318048115868 25.080431818797422 25.614734907382804 25.27051522570391 100-101 23.885941120929267 25.07733944176087 25.90376889577885 25.132950541531013 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 60.0 1 63.5 2 118.5 3 167.5 4 137.0 5 134.5 6 244.5 7 579.5 8 1009.5 9 1206.5 10 1128.0 11 1074.5 12 1149.5 13 1243.5 14 1360.5 15 1463.5 16 1552.0 17 1637.5 18 1719.5 19 1836.0 20 2013.0 21 2306.0 22 2744.0 23 3326.0 24 4090.5 25 5101.0 26 6844.0 27 9607.0 28 13118.0 29 17741.0 30 24259.0 31 31820.0 32 39861.5 33 48428.5 34 58574.5 35 68979.0 36 79935.5 37 92248.0 38 104625.0 39 113149.0 40 120504.5 41 134488.0 42 150191.5 43 166590.5 44 182460.5 45 194490.0 46 205743.0 47 220917.5 48 242136.5 49 258140.5 50 265606.5 51 268786.0 52 264901.5 53 264340.0 54 264459.5 55 254213.0 56 240827.0 57 222606.0 58 200690.5 59 177080.5 60 147151.0 61 114732.5 62 89240.5 63 69593.0 64 52443.5 65 38776.0 66 27368.0 67 20133.0 68 14113.5 69 9168.5 70 6443.5 71 4484.0 72 3079.5 73 2101.5 74 1507.5 75 1075.5 76 679.0 77 333.5 78 156.0 79 76.5 80 33.5 81 21.0 82 13.0 83 13.5 84 13.0 85 9.5 86 7.5 87 3.5 88 2.0 89 1.0 90 1.5 91 1.5 92 0.5 93 0.0 94 0.5 95 1.0 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020872509860003705 2 0.0016995427875829987 3 5.488106918236766E-4 4 0.002602424893486467 5 0.008745563927770848 6 8.67474964495489E-4 7 6.373285453436246E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.00247849989855854 22-23 3.540714140797914E-5 24-25 6.727356867516037E-4 26-27 4.691446236557236E-4 28-29 6.19624974639635E-4 30-31 2.2129463379986963E-4 32-33 0.001478248153783129 34-35 5.753660478796611E-4 36-37 6.461803306956193E-4 38-39 0.0 40-41 2.655535605598436E-4 42-43 0.0 44-45 2.921089166158279E-4 46-47 0.004169190900789543 48-49 0.0012038428078712908 50-51 0.0 52-53 7.966606816795307E-5 54-55 0.0012215463785752805 56-57 0.0 58-59 2.655535605598436E-4 60-61 6.19624974639635E-5 62-63 2.744053459118383E-4 64-65 0.0020182070602548113 66-67 2.1244284844787483E-4 68-69 1.4162856563191656E-4 70-71 0.0 72-73 0.0016641356461750195 74-75 1.8588749239189048E-4 76-77 1.0622142422393742E-4 78-79 0.0 80-81 0.0 82-83 4.6029283830372885E-4 84-85 6.550321160476142E-4 86-87 0.0015048035098391135 88-89 2.6555356055984354E-5 90-91 0.0013100642320952283 92-93 0.005098628362748997 94-95 0.0 96-97 8.940303205514733E-4 98-99 8.851785351994785E-5 100-101 0.0075240175491955675 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5648578.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.99060794269019 #Duplication Level Percentage of deduplicated Percentage of total 1 76.17286713352857 35.794093353422724 2 11.755636128569796 11.048089768690952 3 4.1392209565461595 5.835135274716829 4 2.143752453374997 4.0294492425089885 5 1.2348557159216935 2.9013310406333157 6 0.8538577308565927 2.4073976321710338 7 0.6106207178448955 2.008540712775347 8 0.44582577693320063 1.6759699435690625 9 0.3628521033167297 1.5345576835323262 >10 2.044376409931794 17.54383683603593 >50 0.14574671588952406 4.728669759899644 >100 0.08346101298390765 7.316249842215727 >500 0.004894185208363436 1.5464066652023478 >1k 0.0020329590936866743 1.6302722446257647 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.540714140797914E-5 2 1.770357070398957E-5 0.0 0.0 0.0 3.540714140797914E-5 3 1.770357070398957E-5 0.0 0.0 0.0 3.540714140797914E-5 4 1.770357070398957E-5 0.0 0.0 0.0 3.540714140797914E-5 5 1.770357070398957E-5 0.0 0.0 0.0 3.540714140797914E-5 6 1.770357070398957E-5 0.0 0.0 0.0 3.540714140797914E-5 7 1.770357070398957E-5 0.0 0.0 0.0 3.540714140797914E-5 8 1.770357070398957E-5 0.0 0.0 0.0 3.540714140797914E-5 9 1.770357070398957E-5 0.0 0.0 1.770357070398957E-5 3.540714140797914E-5 10-11 1.770357070398957E-5 0.0 0.0 3.540714140797914E-5 7.081428281595828E-5 12-13 1.770357070398957E-5 0.0 0.0 3.540714140797914E-5 8.851785351994785E-5 14-15 5.311071211196871E-5 0.0 0.0 4.425892675997392E-5 8.851785351994785E-5 16-17 5.311071211196871E-5 0.0 0.0 6.19624974639635E-5 1.0622142422393742E-4 18-19 6.19624974639635E-5 0.0 0.0 7.081428281595828E-5 1.0622142422393742E-4 20-21 7.081428281595828E-5 0.0 0.0 7.081428281595828E-5 1.23924994927927E-4 22-23 8.851785351994785E-5 0.0 0.0 8.851785351994785E-5 1.23924994927927E-4 24-25 1.0622142422393742E-4 0.0 0.0 8.851785351994785E-5 1.23924994927927E-4 26-27 1.0622142422393742E-4 0.0 0.0 1.327767802799218E-4 1.23924994927927E-4 28-29 1.5933213633590615E-4 0.0 0.0 5.842178332316558E-4 1.23924994927927E-4 30-31 1.9473927774388527E-4 8.851785351994785E-6 0.0 0.002513907039966519 1.23924994927927E-4 32-33 1.9473927774388527E-4 1.770357070398957E-5 0.0 0.006116583678228397 1.5048035098391135E-4 34-35 1.9473927774388527E-4 1.770357070398957E-5 0.0 0.01054247635422579 1.770357070398957E-4 36-37 1.9473927774388527E-4 2.6555356055984354E-5 0.0 0.0183320474639812 1.770357070398957E-4 38-39 2.47849989855854E-4 3.540714140797914E-5 0.0 0.03177790941366128 1.9473927774388527E-4 40-41 2.567017752078488E-4 3.540714140797914E-5 0.0 0.046188615966708785 1.9473927774388527E-4 42-43 2.7440534591183837E-4 3.540714140797914E-5 0.0 0.06236082780480326 1.9473927774388527E-4 44-45 3.009607019678227E-4 4.425892675997392E-5 0.0 0.0809495770439923 1.9473927774388527E-4 46-47 3.452196287277966E-4 5.311071211196871E-5 0.0 0.10292856007299536 2.0359106309588007E-4 48-49 3.71774984783781E-4 5.311071211196871E-5 0.0 0.12630612518761358 2.1244284844787483E-4 50-51 3.71774984783781E-4 5.311071211196871E-5 0.0 0.15115308667066296 2.1244284844787483E-4 52-53 3.71774984783781E-4 5.311071211196871E-5 0.0 0.17853165876438282 2.2129463379986963E-4 54-55 3.8947855548777054E-4 5.311071211196871E-5 0.0 0.21012368068565224 2.389982045038592E-4 56-57 3.8947855548777054E-4 5.311071211196871E-5 0.0 0.24821291305528576 2.47849989855854E-4 58-59 3.8947855548777054E-4 5.311071211196871E-5 0.0 0.29023233812120497 2.567017752078488E-4 60-61 3.8947855548777054E-4 5.311071211196871E-5 0.0 0.33827982901183273 2.655535605598436E-4 62-63 3.8947855548777054E-4 5.311071211196871E-5 0.0 0.39153216968943333 3.009607019678227E-4 64-65 4.0718212619176013E-4 5.311071211196871E-5 0.0 0.4499362494418949 3.009607019678227E-4 66-67 4.6029283830372885E-4 7.081428281595828E-5 0.0 0.5190686930409742 3.009607019678227E-4 68-69 4.6029283830372885E-4 7.081428281595828E-5 0.0 0.5972742166258481 3.009607019678227E-4 70-71 4.6914462365572364E-4 7.081428281595828E-5 0.0 0.6868808397440913 3.009607019678227E-4 72-73 4.779964090077184E-4 7.081428281595828E-5 0.0 0.7846665125275778 3.8947855548777054E-4 74-75 4.779964090077184E-4 7.081428281595828E-5 0.0 0.8925786277537462 4.1603391154375487E-4 76-77 4.779964090077184E-4 7.081428281595828E-5 0.0 1.018105795830384 4.4258926759973926E-4 78-79 4.779964090077184E-4 7.081428281595828E-5 0.0 1.161309979254956 4.4258926759973926E-4 80-81 5.665142625276662E-4 7.081428281595828E-5 0.0 1.3166588121824643 4.602928383037288E-4 82-83 5.842178332316558E-4 7.081428281595828E-5 0.0 1.484940103509237 4.95699979711708E-4 84-85 6.373285453436246E-4 7.081428281595828E-5 0.0 1.6748994171630454 4.95699979711708E-4 86-87 6.727356867516037E-4 7.081428281595828E-5 0.0 1.8846867300053218 5.045517650637027E-4 88-89 6.727356867516037E-4 7.081428281595828E-5 0.0 2.1221801309993418 5.134035504156976E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4625 0.0 41.403603 1 GTATCAA 8490 0.0 40.96832 1 TCAACGC 9750 0.0 34.49546 4 ATCAACG 9890 0.0 34.00715 3 CAACGCA 10265 0.0 32.717945 5 AACGCAG 10770 0.0 31.4454 6 TATCAAC 10840 0.0 31.245934 2 ACGCAGA 12405 0.0 27.147202 7 GTACATG 15970 0.0 25.498856 1 CGCAGAG 13265 0.0 25.422995 8 TACATGG 16860 0.0 23.301355 2 ACATGGG 16805 0.0 22.755724 3 GCAGAGT 16305 0.0 20.682981 9 CATGGGG 14250 0.0 20.135174 4 GCTTATA 2950 0.0 19.167688 1 AGAGTAC 14410 0.0 18.903622 10-11 TTATAGG 2810 0.0 18.596004 3 TATAAGC 2850 0.0 18.168325 3 CCTATAA 2910 0.0 18.124865 1 CAGAGTA 14815 0.0 17.296785 10-11 >>END_MODULE