FastQCFastQC Report
Fri 27 May 2016
SRR940226_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940226_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5760873
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA80050.041.015541
GGTATCA44250.040.787611
ATCAACG92150.037.209613
TCAACGC94100.036.488054
CAACGCA96300.035.603615
AACGCAG102450.033.5584686
TATCAAC98700.032.680932
ACGCAGA118850.028.8091557
CGCAGAG125300.027.2131678
GTACATG153850.025.6152951
ACATGGG168600.023.9427813
TACATGG162800.023.7347012
GCAGAGT154800.021.6579259
CATGGGG147400.020.3620534
AGAGTAC133650.019.38229210-11
CCTATAA30300.018.0599251
TATAAGC31350.017.7239933
CAGAGTA143450.017.49539610-11
GAGTACA117800.017.3179841
AGTACAT112050.016.5355152
Produced by FastQC (version 0.11.5)