##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940226_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5760873 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.47520228270958 33.0 31.0 34.0 28.0 34.0 2 29.67245381038603 33.0 31.0 34.0 16.0 34.0 3 31.032890848314135 33.0 31.0 34.0 25.0 34.0 4 35.1057450841218 37.0 35.0 37.0 32.0 37.0 5 35.41255483326919 37.0 35.0 37.0 33.0 37.0 6 35.641637821212164 37.0 35.0 37.0 35.0 37.0 7 35.69807458001591 37.0 36.0 37.0 35.0 37.0 8 35.72956477256138 37.0 37.0 37.0 35.0 37.0 9 37.49723835258996 39.0 38.0 39.0 35.0 39.0 10-11 37.42917740071687 39.0 38.0 39.0 35.0 39.0 12-13 37.35511527506334 39.0 38.0 39.0 35.0 39.0 14-15 38.76450904229272 41.0 39.0 41.0 36.0 41.0 16-17 38.70194960034703 41.0 39.0 41.0 35.0 41.0 18-19 38.63700900887765 41.0 39.0 41.0 35.0 41.0 20-21 38.54703384365529 40.5 39.0 41.0 34.5 41.0 22-23 38.435412653603024 40.0 39.0 41.0 34.0 41.0 24-25 38.31933016055032 40.0 38.0 41.0 34.0 41.0 26-27 38.182631347714135 40.0 38.0 41.0 34.0 41.0 28-29 38.018943049083006 40.0 38.0 41.0 33.5 41.0 30-31 37.86157384479748 40.0 38.0 41.0 33.0 41.0 32-33 37.9404068619461 40.0 38.0 41.0 33.5 41.0 34-35 37.98696933607111 40.0 38.0 41.0 33.5 41.0 36-37 38.093665491323975 40.0 38.0 41.0 34.0 41.0 38-39 38.028669873472296 40.0 38.0 41.0 33.0 41.0 40-41 37.905528988401585 40.0 38.0 41.0 33.0 41.0 42-43 37.66502941481265 40.0 38.0 41.0 33.0 41.0 44-45 37.620534856435825 40.0 38.0 41.0 33.0 41.0 46-47 37.449133490705314 40.0 38.0 41.0 32.5 41.0 48-49 37.25802886125072 40.0 37.5 41.0 32.0 41.0 50-51 36.34809758868144 39.0 36.0 40.5 31.0 40.5 52-53 36.44538232313054 39.0 36.0 40.5 31.0 41.0 54-55 36.75532458361779 40.0 36.0 41.0 31.0 41.0 56-57 36.59764848140203 39.5 36.0 41.0 31.0 41.0 58-59 36.332952314692584 39.0 35.5 41.0 31.0 41.0 60-61 36.021473915498575 39.0 35.0 41.0 30.0 41.0 62-63 35.671485380774755 38.5 35.0 40.5 29.5 41.0 64-65 35.27516194160156 37.5 35.0 40.0 29.0 41.0 66-67 34.899995538870584 37.0 35.0 39.5 29.0 41.0 68-69 34.50846972325201 36.5 34.5 39.0 29.0 41.0 70-71 34.08577831866802 36.0 34.0 39.0 28.0 40.5 72-73 33.659357965363924 35.0 34.0 37.5 27.5 40.0 74-75 33.2256285635875 35.0 34.0 37.0 26.5 39.0 76-77 32.765211800364284 35.0 33.5 37.0 26.0 39.0 78-79 32.3363859956642 35.0 33.0 36.0 25.5 38.5 80-81 32.000795886317924 35.0 33.0 36.0 25.0 37.0 82-83 31.662770729366883 35.0 33.0 35.0 24.0 37.0 84-85 31.376571571704496 35.0 32.5 35.0 23.5 36.0 86-87 31.104964560058868 35.0 32.0 35.0 22.0 36.0 88-89 30.81912732670899 35.0 32.0 35.0 20.0 36.0 90-91 30.575221758924386 34.0 32.0 35.0 18.5 35.0 92-93 30.307174450816742 34.0 31.0 35.0 17.5 35.0 94-95 29.988298040939284 34.0 31.0 35.0 8.5 35.0 96-97 29.63378684098747 34.0 31.0 35.0 2.0 35.0 98-99 29.201496891182984 34.0 30.5 35.0 2.0 35.0 100-101 27.346776261167363 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6617828080636343 1101 2 1.8889892921607938 1101 3 1.0153260938625799 1101 4 0.600536395401619 1101 5 0.43802568847202394 1101 6 0.35649598027764995 1101 7 0.32058663057085823 1101 8 0.31897684893684897 1101 9 0.373470263016614 1101 10-11 0.3826714004121783 1101 12-13 0.39371138927161553 1101 14-15 0.49687904978355846 1101 16-17 0.513475299787217 1101 18-19 0.5126133411399039 1101 20-21 0.4633962653239365 1101 22-23 0.4782056982375096 1101 24-25 0.4411057761695858 1101 26-27 0.5219513389924089 1101 28-29 0.5742534230570797 1101 30-31 0.6139221531563948 1101 32-33 0.6067208580428414 1101 34-35 0.6293216206585086 1101 36-37 0.6928131518904976 1101 38-39 0.7138443794796316 1101 40-41 0.7114913603384068 1101 42-43 0.8068490370847314 1101 44-45 0.6863503967366213 1101 46-47 0.6430574219876846 1101 48-49 0.5799921643589769 1101 50-51 0.5408703427979518 1101 52-53 0.6090158773538974 1101 54-55 0.7283054479745772 1101 56-57 0.758356214282216 1101 58-59 0.7622967819669313 1101 60-61 0.7772383320663323 1101 62-63 0.8232439887720275 1101 64-65 0.790732489274582 1101 66-67 0.7674547215847056 1101 68-69 0.7898978929545493 1101 70-71 0.7403705911397722 1101 72-73 0.7086490261952321 1101 74-75 0.6902989083649729 1101 76-77 0.7471685704794808 1101 78-79 0.7763077297381216 1101 80-81 0.6861560682316252 1101 82-83 0.7009093291965662 1101 84-85 0.6992167467007242 1101 86-87 0.6191579493235508 1101 88-89 0.6850684430214038 1101 90-91 0.6067453218611298 1101 92-93 0.6832661944193248 1101 94-95 0.6556048549173923 1101 96-97 0.6076855478538796 1101 98-99 0.5607711096771162 1101 100-101 0.6156361692893597 1102 1 0.8536990727437228 1102 2 2.679092332430976 1102 3 1.4152217321719114 1102 4 0.6906581448925664 1102 5 0.46861242969646355 1102 6 0.3228797844597082 1102 7 0.22106447889708392 1102 8 0.23575570551126646 1102 9 0.23683887347973354 1102 10-11 0.20554861253767598 1102 12-13 0.31995017736766584 1102 14-15 0.3577545148428811 1102 16-17 0.2984099723344187 1102 18-19 0.32170351478676196 1102 20-21 0.3525264697254471 1102 22-23 0.3767515687548695 1102 24-25 0.3344830609077931 1102 26-27 0.3710311636926775 1102 28-29 0.32108844654390367 1102 30-31 0.29322899179089035 1102 32-33 0.3129010500805691 1102 34-35 0.36494223255033376 1102 36-37 0.41525804582142456 1102 38-39 0.39276155170956173 1102 40-41 0.36455110293615434 1102 42-43 0.49072655143610433 1102 44-45 0.36023875720267995 1102 46-47 0.2595671318116004 1102 48-49 0.34217954265637474 1102 50-51 0.29521260465988064 1102 52-53 0.3076313732114926 1102 54-55 0.4492810793235549 1102 56-57 0.46267169884449544 1102 58-59 0.5070009604031824 1102 60-61 0.46580220094129743 1102 62-63 0.5836674015519421 1102 64-65 0.6314118084333913 1102 66-67 0.5108867602312372 1102 68-69 0.5470553692071363 1102 70-71 0.5023681515271647 1102 72-73 0.5107266219251727 1102 74-75 0.5619734970128789 1102 76-77 0.5867359121172626 1102 78-79 0.5798761756124335 1102 80-81 0.6258178500036067 1102 82-83 0.5417120681567091 1102 84-85 0.5378167910969616 1102 86-87 0.5164422557762265 1102 88-89 0.45144697024375446 1102 90-91 0.40169466086741323 1102 92-93 0.36606487578995583 1102 94-95 0.3761530961416142 1102 96-97 0.3143051231065961 1102 98-99 0.2338909311152655 1102 100-101 0.2978729198684107 1103 1 0.8660232616819599 1103 2 2.646095689416235 1103 3 1.4133917415460182 1103 4 0.8071121785300477 1103 5 0.5461897544135823 1103 6 0.4072612729221916 1103 7 0.308763480601165 1103 8 0.2906572880596059 1103 9 0.255715384041558 1103 10-11 0.2867561148036586 1103 12-13 0.28121872613733956 1103 14-15 0.41039204769934656 1103 16-17 0.36608754199770743 1103 18-19 0.37424532966486623 1103 20-21 0.464419288753696 1103 22-23 0.3632718581373453 1103 24-25 0.388641431295234 1103 26-27 0.44665840100890364 1103 28-29 0.4273133035177423 1103 30-31 0.4268966191651984 1103 32-33 0.43034652694347386 1103 34-35 0.4077886416051726 1103 36-37 0.44027460177009203 1103 38-39 0.4844644504266924 1103 40-41 0.4959331973240708 1103 42-43 0.6043311613683642 1103 44-45 0.5069401878669524 1103 46-47 0.39507678112235567 1103 48-49 0.47412309820385445 1103 50-51 0.3907327736294022 1103 52-53 0.43542550780281175 1103 54-55 0.6136230904434825 1103 56-57 0.6307224321686817 1103 58-59 0.6082784526134546 1103 60-61 0.5758778760542356 1103 62-63 0.5442355882640371 1103 64-65 0.5588477627552848 1103 66-67 0.507684484646127 1103 68-69 0.5509130932852244 1103 70-71 0.4863812189615402 1103 72-73 0.5335608013109123 1103 74-75 0.4681610918696961 1103 76-77 0.6060797300970862 1103 78-79 0.6601186269884636 1103 80-81 0.5955864947242304 1103 82-83 0.6129930070129497 1103 84-85 0.5810562396043188 1103 86-87 0.5920046438292914 1103 88-89 0.5790925356224967 1103 90-91 0.5518514905814698 1103 92-93 0.43916723525143553 1103 94-95 0.42356824145488403 1103 96-97 0.32533968202277563 1103 98-99 0.32041331058433187 1103 100-101 0.3763188120195444 1104 1 0.8802746976232285 1104 2 2.6361069565590114 1104 3 1.3797911721901706 1104 4 0.8542079877136786 1104 5 0.5293041348217926 1104 6 0.34494207372393504 1104 7 0.2855186412851012 1104 8 0.253841703410302 1104 9 0.27636434668850285 1104 10-11 0.3069957795800704 1104 12-13 0.3252867841135725 1104 14-15 0.29713323530053515 1104 16-17 0.3239024596165052 1104 18-19 0.37439601465889893 1104 20-21 0.4130943399220399 1104 22-23 0.379594854127987 1104 24-25 0.38529688950215046 1104 26-27 0.44094625955409583 1104 28-29 0.48313987689142834 1104 30-31 0.41702300286227256 1104 32-33 0.478331433014759 1104 34-35 0.4665207098626709 1104 36-37 0.5212403843442246 1104 38-39 0.5256200041565577 1104 40-41 0.47903624864629535 1104 42-43 0.5252637097834949 1104 44-45 0.4062419086491573 1104 46-47 0.37191078898457874 1104 48-49 0.4089126021561569 1104 50-51 0.35934995028425476 1104 52-53 0.44305868732138975 1104 54-55 0.4826764776608812 1104 56-57 0.5109653538535639 1104 58-59 0.4484933335879546 1104 60-61 0.48112074883658806 1104 62-63 0.5152620295356911 1104 64-65 0.5307450276694112 1104 66-67 0.4810438210844481 1104 68-69 0.4881958111965261 1104 70-71 0.32451325970321676 1104 72-73 0.29755585759120606 1104 74-75 0.33874367748335743 1104 76-77 0.3953262594845697 1104 78-79 0.4472051968542061 1104 80-81 0.4755101806969648 1104 82-83 0.5086722403256196 1104 84-85 0.419036274983668 1104 86-87 0.3794280054152459 1104 88-89 0.3753145544101919 1104 90-91 0.3381062610232135 1104 92-93 0.20250942646293524 1104 94-95 0.16860644411087833 1104 96-97 0.2005514788777596 1104 98-99 0.11568906542747825 1104 100-101 0.022796297621006545 1105 1 0.637207647803784 1105 2 2.3686080061394676 1105 3 1.1378362942450373 1105 4 0.613703128331899 1105 5 0.3962073964130255 1105 6 0.24245512718192685 1105 7 0.20298663147751483 1105 8 0.17062157140279766 1105 9 0.09899173572213016 1105 10-11 0.09648634805329692 1105 12-13 0.12539723767930155 1105 14-15 -0.0030317900587348845 1105 16-17 0.03005597249998715 1105 18-19 0.08454777408802983 1105 20-21 0.09176295110823673 1105 22-23 0.16983751010011616 1105 24-25 0.0764319125162416 1105 26-27 0.11430020747828706 1105 28-29 0.10329204528950697 1105 30-31 0.218327741598209 1105 32-33 0.2312896044379258 1105 34-35 0.20744086442736887 1105 36-37 0.25836037072044604 1105 38-39 0.03767835763481031 1105 40-41 0.13360690793618346 1105 42-43 0.1708937328327309 1105 44-45 0.13883042326756367 1105 46-47 0.014645120202139594 1105 48-49 0.0022621440789905023 1105 50-51 0.01375040820165907 1105 52-53 0.07910902820339771 1105 54-55 0.19151555860542402 1105 56-57 0.179275244959193 1105 58-59 0.18706468045687785 1105 60-61 0.1844847101097855 1105 62-63 0.1689956763692848 1105 64-65 0.27257036150628977 1105 66-67 0.1148716234323146 1105 68-69 0.11354094264489589 1105 70-71 0.09238555403816662 1105 72-73 0.08897808543675012 1105 74-75 0.11711982794813736 1105 76-77 0.11311985924838552 1105 78-79 0.2040790062912876 1105 80-81 0.17572041629502166 1105 82-83 0.17666153811955 1105 84-85 0.28488099394851574 1105 86-87 0.2575058801314434 1105 88-89 0.18686342204548012 1105 90-91 0.007612737749369103 1105 92-93 -0.024806679692623845 1105 94-95 -0.31045845159245644 1105 96-97 -0.3964566896378976 1105 98-99 -0.4059837325782887 1105 100-101 -0.2557675951498908 1106 1 0.7420420716201193 1106 2 2.553860310826785 1106 3 1.3296554155371432 1106 4 0.7721608535112523 1106 5 0.46125547811484324 1106 6 0.30359053377404877 1106 7 0.2476758398767842 1106 8 0.1850853992900241 1106 9 0.1865882817283122 1106 10-11 0.1978818454918283 1106 12-13 0.21002811693940515 1106 14-15 0.20082689470358872 1106 16-17 0.2236617135892871 1106 18-19 0.1588506091334736 1106 20-21 0.2267358218264377 1106 22-23 0.2430460229673983 1106 24-25 0.25016319457399305 1106 26-27 0.17005496003304899 1106 28-29 0.18794210989806004 1106 30-31 0.20092156599863387 1106 32-33 0.23964950958404785 1106 34-35 0.23280072381896133 1106 36-37 0.19448982133474146 1106 38-39 0.18859205985484806 1106 40-41 0.23974468903072932 1106 42-43 0.12891470734114563 1106 44-45 0.20378068493614876 1106 46-47 0.18661802405293315 1106 48-49 -0.006846746973160123 1106 50-51 -7.583695685724479E-4 1106 52-53 0.13921642513342647 1106 54-55 0.28393365404937043 1106 56-57 0.2794549024944075 1106 58-59 0.23037473682961718 1106 60-61 0.255367267693444 1106 62-63 0.322698353956028 1106 64-65 0.23027130673180096 1106 66-67 0.12818944229800877 1106 68-69 0.18287545087044776 1106 70-71 0.17271099497774856 1106 72-73 0.18426693063970845 1106 74-75 0.1340096399916817 1106 76-77 0.21270374544898374 1106 78-79 0.25508352336798623 1106 80-81 0.2508995295090912 1106 82-83 0.2563935585607773 1106 84-85 -0.023862034401940235 1106 86-87 0.03095510916758215 1106 88-89 0.17960769996797055 1106 90-91 0.06416868370853024 1106 92-93 0.035969159390266014 1106 94-95 -0.014535148809475373 1106 96-97 0.058706393712657956 1106 98-99 -0.061493666833758454 1106 100-101 -0.2294468590383616 1107 1 0.7559699113634508 1107 2 2.550081868790407 1107 3 1.3631943162445914 1107 4 0.7220520673052491 1107 5 0.4452015975695076 1107 6 0.277526870786005 1107 7 0.18859977420287777 1107 8 0.1721835518144843 1107 9 0.12662650431743572 1107 10-11 0.160536606910604 1107 12-13 0.24652807384826758 1107 14-15 0.19282600566551622 1107 16-17 0.2348618242654581 1107 18-19 0.1908759376466449 1107 20-21 0.19408529392229212 1107 22-23 0.24945586649366902 1107 24-25 0.2349516020013951 1107 26-27 0.2285526520116008 1107 28-29 0.22970867994987287 1107 30-31 0.2074133733918515 1107 32-33 0.20767430286001343 1107 34-35 0.17294990985017478 1107 36-37 0.21010102744793357 1107 38-39 0.16575997465233883 1107 40-41 0.23538521223265008 1107 42-43 0.38482286426543766 1107 44-45 0.21432612793565653 1107 46-47 0.21571292886689264 1107 48-49 0.21715499783964987 1107 50-51 0.2150843039563881 1107 52-53 0.27588557143008074 1107 54-55 0.3793537169775476 1107 56-57 0.4317439638486249 1107 58-59 0.41580184553568955 1107 60-61 0.3739401650352505 1107 62-63 0.44701658422595614 1107 64-65 0.3737505904816132 1107 66-67 0.45914970925134213 1107 68-69 0.410264128335605 1107 70-71 0.3900842654736252 1107 72-73 0.4468004321040482 1107 74-75 0.4197509359612255 1107 76-77 0.42495429693985187 1107 78-79 0.458336057050893 1107 80-81 0.47487876109595106 1107 82-83 0.5083116128374634 1107 84-85 0.4547078541205529 1107 86-87 0.4625648123830892 1107 88-89 0.4692150263674115 1107 90-91 0.5525408217392531 1107 92-93 0.4399834081908942 1107 94-95 0.31762831323782237 1107 96-97 0.4458519313614566 1107 98-99 0.4836163667470821 1107 100-101 0.605501242608323 1108 1 0.7339377653985792 1108 2 2.5210492527262076 1108 3 1.3191136874675884 1108 4 0.737449531296555 1108 5 0.4511471325009353 1108 6 0.33121982675971395 1108 7 0.205029721117441 1108 8 0.13251227014863076 1108 9 0.14657696901629436 1108 10-11 0.1683006571743917 1108 12-13 0.16290106311653574 1108 14-15 0.21531350120673665 1108 16-17 0.18878623638138947 1108 18-19 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11899.0 4 9758.0 5 8038.0 6 7344.0 7 7264.0 8 7716.0 9 8560.0 10 9658.0 11 11026.0 12 12186.0 13 13322.0 14 14380.0 15 15899.0 16 18954.0 17 21883.0 18 20973.0 19 18368.0 20 18904.0 21 20519.0 22 21593.0 23 23544.0 24 26993.0 25 31508.0 26 36491.0 27 43608.0 28 52877.0 29 64854.0 30 80160.0 31 100575.0 32 129844.0 33 175371.0 34 255077.0 35 405423.0 36 770772.0 37 1534665.0 38 1509124.0 39 195632.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.85777037187789 17.79028584566293 13.430589021409425 24.921354761049752 2 17.594404700916126 20.50281662183344 36.8631097393495 25.039668937900935 3 18.713218786559835 23.66894850395719 28.744569307118073 28.873263402364902 4 13.509186275143056 16.214749713677698 35.76146452555231 34.51459948562693 5 15.347763712581964 36.07726199476512 33.58417892938667 14.99079536326626 6 33.855326391635245 34.357447337455206 17.537011954169376 14.250214316740179 7 30.17812851684806 30.64868517410579 20.85264109320956 18.320545215836592 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25.766740845201248 24.314751371695227 32-33 24.362783166604085 25.50246024218246 25.772338065709192 24.362418525504257 34-35 24.76827243887729 25.533279200554997 25.52379887435885 24.17464948620886 36-37 24.607942591167383 25.40179367876039 25.576299930938895 24.413963799133327 38-39 24.47023495741403 25.52369317476051 25.862433089014797 24.143638778810665 40-41 24.67550685114152 25.265253710505124 25.8458687815842 24.21337065676915 42-43 24.685341333041677 25.291195980702092 25.657476029519756 24.365986656736474 44-45 24.522239857883807 25.480598785303783 25.54734596410913 24.449815392703282 46-47 24.574051117424904 25.487827669596356 25.452558887711334 24.485562325267406 48-49 24.67733801365731 25.5556192098134 25.37194774216901 24.395095034360278 50-51 24.427194826422596 25.60096773650259 25.48968915093693 24.48214828613789 52-53 24.789750888592323 25.41273340251214 25.347350924564836 24.450164784330706 54-55 24.551859472853057 25.278615367734787 25.541015209954598 24.628509949457563 56-57 24.501403627503358 25.668873776654728 25.594104504056535 24.23561809178538 58-59 24.653778954337174 25.347037082629647 25.541097618335346 24.458086344697836 60-61 24.677298145809317 25.27809561525489 25.712890339313848 24.33171589962194 62-63 24.71596167262269 25.40260032496249 25.615919897684392 24.26551810473043 64-65 24.530981689810602 25.32164560530019 25.56542496492666 24.581947739962548 66-67 24.425573042404057 25.616037348744037 25.438415188804058 24.51997442004785 68-69 24.294376373862228 25.399481787609655 25.76043705604876 24.545704782479355 70-71 24.29114530051047 25.405703129781166 25.599638535861903 24.703513033846463 72-73 24.498091155579 25.384666202459133 25.506227693362245 24.61101494859962 74-75 24.355440015599285 25.441017467759195 25.513917724199324 24.689624792442196 76-77 24.51313163794006 25.320028384846992 25.579152104268992 24.587687872943953 78-79 24.41110160994179 25.426749601746675 25.543113364383345 24.61903542392819 80-81 24.3078779989161 25.5131781772132 25.685311831543228 24.49363199232747 82-83 24.433890917879026 25.468424144820588 25.525361450761512 24.57232348653887 84-85 24.383631922248245 25.385390642497143 25.485080392439414 24.7458970428152 86-87 24.504056875958096 25.251615553554217 25.72766539207265 24.516662178415032 88-89 24.31782035878239 25.243719919159368 25.669947634161417 24.768512087896823 90-91 24.317973396743536 25.464864249222103 25.738322488526403 24.478839865507958 92-93 24.400530093337654 25.27380529515073 25.789155487823145 24.536509123688475 94-95 24.34687631514454 25.215577320989667 25.669198518023702 24.76834784584209 96-97 24.564964975537425 25.174132136134364 25.536571570323048 24.724331318005166 98-99 24.22383232172248 25.098711538200945 25.66348850953916 25.013967630537408 100-101 24.19713971496512 25.074439361863455 25.92516996285545 24.803250960315975 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247.0 1 168.0 2 139.5 3 203.5 4 182.5 5 174.5 6 287.5 7 578.0 8 1015.0 9 1244.0 10 1156.0 11 1150.5 12 1253.5 13 1308.0 14 1408.0 15 1524.5 16 1597.5 17 1684.5 18 1798.0 19 1924.0 20 2108.5 21 2441.0 22 2914.0 23 3476.0 24 4266.5 25 5383.0 26 7106.5 27 9840.0 28 13424.0 29 18200.5 30 24926.5 31 33003.0 32 41201.5 33 50359.0 34 61042.0 35 71271.0 36 81973.5 37 94575.5 38 107524.5 39 116431.0 40 123892.0 41 137640.0 42 153099.0 43 169703.5 44 185860.0 45 197492.0 46 209706.0 47 224980.5 48 245105.5 49 261433.0 50 269047.5 51 271573.5 52 269016.5 53 268624.0 54 266730.0 55 256632.0 56 243744.0 57 225625.0 58 202710.5 59 178256.0 60 149701.0 61 118336.5 62 91973.0 63 71839.5 64 54424.0 65 40402.0 66 29361.5 67 21709.5 68 15359.5 69 10411.0 70 7496.5 71 5270.5 72 3813.0 73 2739.0 74 1917.0 75 1392.5 76 989.5 77 605.0 78 363.0 79 273.0 80 206.5 81 157.5 82 133.5 83 110.5 84 84.0 85 69.5 86 56.0 87 50.0 88 51.5 89 46.5 90 41.0 91 30.0 92 30.0 93 29.5 94 27.0 95 26.5 96 22.0 97 21.5 98 19.5 99 16.5 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03647016693476839 2 1.095146516856039 3 0.025673192240134438 4 0.028276964272602435 5 0.03157507551372856 6 0.012272445513032486 7 0.019076969757882183 8 0.02063923297736298 9 0.011473955423075635 10-11 0.010727540773768142 12-13 0.025456211237428774 14-15 0.015622632194807974 16-17 0.02707054989755893 18-19 0.03132337755058999 20-21 0.04471544503758371 22-23 0.03253847116574172 24-25 0.009755465881646757 26-27 0.016238858242492066 28-29 0.02502224923201744 30-31 0.01945017708253593 32-33 0.030993566426477378 34-35 0.027383002541455086 36-37 0.02569922996045912 38-39 0.012176973871841993 40-41 0.013444142927643085 42-43 0.01150867238350854 44-45 0.018729800153553116 46-47 0.01425999149781639 48-49 0.010206786367274543 50-51 0.025239230234723107 52-53 0.01596980179913704 54-55 0.016681499488011627 56-57 0.020031686169787115 58-59 0.023086778687882895 60-61 0.017853196902622225 62-63 0.024978853031476306 64-65 0.02007508237032825 66-67 0.01550980207340103 68-69 0.015266783350370683 70-71 0.016759612648985664 72-73 0.02146376078764451 74-75 0.028363756673684702 76-77 0.025378098076454733 78-79 0.023399231331779055 80-81 0.019172441399072676 82-83 0.01786187614273045 84-85 0.017410555657102665 86-87 0.024163004461303 88-89 0.024180362941519453 90-91 0.019563007203942877 92-93 0.03930827845015851 94-95 0.059470153221569025 96-97 0.07507542693616055 98-99 0.08170636637884571 100-101 0.07245429642347609 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5760873.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.779975472529706 #Duplication Level Percentage of deduplicated Percentage of total 1 77.57158355415852 38.615115266912994 2 11.034052531141809 10.985497287256871 3 4.034228894402669 6.024714462418064 4 2.003388912135282 3.989146036321292 5 1.2110642567362215 3.0143374497993265 6 0.8263608212628208 2.468173284835364 7 0.5562274477779141 1.938229209527265 8 0.4050997873578574 1.613268598288091 9 0.316265060723742 1.4169300269081395 >10 1.8409048659689575 16.64255971249831 >50 0.12744434161843865 4.372003882855843 >100 0.0677517742809791 6.26846112953591 >500 0.003999606425134846 1.3224822661810336 >1k 0.0016281460097133725 1.3290813866615494 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.471696043290661E-5 2 0.0 0.0 0.0 0.0 3.471696043290661E-5 3 0.0 0.0 0.0 0.0 3.471696043290661E-5 4 0.0 0.0 0.0 1.7358480216453305E-5 3.471696043290661E-5 5 0.0 1.7358480216453305E-5 0.0 1.7358480216453305E-5 3.471696043290661E-5 6 3.471696043290661E-5 1.7358480216453305E-5 0.0 3.471696043290661E-5 5.2075440649359915E-5 7 3.471696043290661E-5 1.7358480216453305E-5 0.0 3.471696043290661E-5 5.2075440649359915E-5 8 3.471696043290661E-5 1.7358480216453305E-5 0.0 3.471696043290661E-5 5.2075440649359915E-5 9 3.471696043290661E-5 1.7358480216453305E-5 0.0 3.471696043290661E-5 5.2075440649359915E-5 10-11 6.943392086581322E-5 1.7358480216453305E-5 0.0 3.471696043290661E-5 6.943392086581322E-5 12-13 7.811316097403987E-5 1.7358480216453305E-5 0.0 3.471696043290661E-5 8.679240108226653E-5 14-15 8.679240108226653E-5 1.7358480216453305E-5 0.0 3.471696043290661E-5 9.547164119049318E-5 16-17 8.679240108226653E-5 1.7358480216453305E-5 0.0 4.339620054113326E-5 1.0415088129871983E-4 18-19 8.679240108226653E-5 1.7358480216453305E-5 0.0 6.075468075758657E-5 1.0415088129871983E-4 20-21 8.679240108226653E-5 1.7358480216453305E-5 0.0 8.679240108226653E-5 1.0415088129871983E-4 22-23 8.679240108226653E-5 1.7358480216453305E-5 0.0 9.547164119049318E-5 1.2150936151517314E-4 24-25 1.5622632194807975E-4 1.7358480216453305E-5 0.0 1.4754708183985308E-4 1.3886784173162644E-4 26-27 1.5622632194807975E-4 1.7358480216453305E-5 0.0 2.603772032467996E-4 1.3886784173162644E-4 28-29 1.649055620563064E-4 1.7358480216453305E-5 0.0 7.637731295239454E-4 1.3886784173162644E-4 30-31 1.7358480216453305E-4 1.7358480216453305E-5 0.0 0.002499621151169276 1.3886784173162644E-4 32-33 1.7358480216453305E-4 1.7358480216453305E-5 0.0 0.005754336191754271 1.3886784173162644E-4 34-35 1.9094328238098636E-4 1.7358480216453305E-5 0.0 0.01013735244640873 1.649055620563064E-4 36-37 2.0830176259743966E-4 1.7358480216453305E-5 0.0 0.017445272617535573 1.9094328238098636E-4 38-39 2.603772032467996E-4 1.7358480216453305E-5 0.0 0.030585642141390722 2.0830176259743966E-4 40-41 2.777356834632529E-4 1.7358480216453305E-5 0.0 0.045496576647324115 2.2566024281389297E-4 42-43 2.777356834632529E-4 1.7358480216453305E-5 0.0 0.06154449160743519 2.3433948292211963E-4 44-45 3.124526438961595E-4 1.7358480216453305E-5 0.0 0.08069957452629142 2.516979631385729E-4 46-47 4.2528276530310596E-4 1.7358480216453305E-5 0.0 0.10323956108735603 2.777356834632529E-4 48-49 4.339620054113326E-4 1.7358480216453305E-5 0.0 0.12694256582292301 2.777356834632529E-4 50-51 4.339620054113326E-4 1.7358480216453305E-5 0.0 0.1530497200684688 2.9509416367970616E-4 52-53 4.339620054113326E-4 1.7358480216453305E-5 0.0 0.18146555218280286 3.471696043290661E-4 54-55 4.339620054113326E-4 3.471696043290661E-5 0.0 0.21400402334854457 3.645280845455194E-4 56-57 4.686789658442392E-4 3.471696043290661E-5 0.0 0.25240966082744754 3.645280845455194E-4 58-59 4.8603744606069254E-4 3.471696043290661E-5 0.0 0.2958058613685808 3.645280845455194E-4 60-61 4.8603744606069254E-4 3.471696043290661E-5 0.0 0.34324658780014766 3.818865647619727E-4 62-63 4.8603744606069254E-4 3.471696043290661E-5 0.0 0.39506165124626075 3.99245044978426E-4 64-65 5.033959262771458E-4 3.471696043290661E-5 0.0 0.4532038807312711 3.99245044978426E-4 66-67 5.033959262771458E-4 3.471696043290661E-5 0.0 0.5201208219656985 3.99245044978426E-4 68-69 5.033959262771458E-4 3.471696043290661E-5 0.0 0.5974354928497816 3.99245044978426E-4 70-71 5.033959262771458E-4 3.471696043290661E-5 0.0 0.6830475172773294 4.079242850866527E-4 72-73 5.294336466018258E-4 3.471696043290661E-5 0.0 0.7775384043355928 4.339620054113326E-4 74-75 5.72829847142959E-4 3.471696043290661E-5 0.0 0.884648906511218 4.339620054113326E-4 76-77 5.901883273594123E-4 3.471696043290661E-5 0.0 1.008406191214422 4.5132048562778593E-4 78-79 5.901883273594123E-4 3.471696043290661E-5 0.0 1.147838183553083 4.5132048562778593E-4 80-81 6.422637680087723E-4 3.471696043290661E-5 0.0 1.304793561670254 4.5132048562778593E-4 82-83 6.422637680087723E-4 3.471696043290661E-5 0.0 1.4718862922338334 4.686789658442392E-4 84-85 6.509430081169989E-4 3.471696043290661E-5 0.0 1.659088822128174 4.686789658442392E-4 86-87 6.596222482252255E-4 3.471696043290661E-5 0.0 1.8663924721131675 4.773582059524659E-4 88-89 6.596222482252255E-4 3.471696043290661E-5 0.0 2.0963402595405247 5.033959262771458E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 8005 0.0 41.01554 1 GGTATCA 4425 0.0 40.78761 1 ATCAACG 9215 0.0 37.20961 3 TCAACGC 9410 0.0 36.48805 4 CAACGCA 9630 0.0 35.60361 5 AACGCAG 10245 0.0 33.558468 6 TATCAAC 9870 0.0 32.68093 2 ACGCAGA 11885 0.0 28.809155 7 CGCAGAG 12530 0.0 27.213167 8 GTACATG 15385 0.0 25.615295 1 ACATGGG 16860 0.0 23.942781 3 TACATGG 16280 0.0 23.734701 2 GCAGAGT 15480 0.0 21.657925 9 CATGGGG 14740 0.0 20.362053 4 AGAGTAC 13365 0.0 19.382292 10-11 CCTATAA 3030 0.0 18.059925 1 TATAAGC 3135 0.0 17.723993 3 CAGAGTA 14345 0.0 17.495396 10-11 GAGTACA 11780 0.0 17.317984 1 AGTACAT 11205 0.0 16.535515 2 >>END_MODULE