FastQCFastQC Report
Fri 27 May 2016
SRR940226_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940226_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5760873
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84050.042.7895431
GGTATCA46000.040.1764221
TCAACGC97000.036.1902734
ATCAACG97100.035.9573173
CAACGCA99950.035.2147335
AACGCAG105950.033.3998376
TATCAAC106800.033.1368872
ACGCAGA123100.028.4374667
CGCAGAG131100.026.6659228
GTACATG163150.026.1498241
TACATGG171700.024.4019762
ACATGGG170550.023.7862153
GCAGAGT162250.021.3121919
CATGGGG145850.020.8771294
CCTATAA29750.019.8022331
TATAAGC28850.019.7585283
AGAGTAC143550.018.72814210-11
TTATAGG29350.018.450833
GCTTATA30250.018.0614221
GTATAGG13700.017.6859781
Produced by FastQC (version 0.11.5)