FastQCFastQC Report
Fri 27 May 2016
SRR940225_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940225_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5378565
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77250.042.3070871
GGTATCA42450.042.0758931
ATCAACG88550.036.421343
TCAACGC89450.036.054894
CAACGCA92300.034.838035
AACGCAG96400.033.4065326
TATCAAC96950.032.8752942
ACGCAGA113550.028.2354957
CGCAGAG120650.026.5345258
GTACATG150700.026.4152031
TACATGG159700.024.1218662
ACATGGG164500.023.6190363
GCAGAGT146450.021.5025929
CATGGGG137100.020.8907784
AGAGTAC129400.018.64731410-11
CAGAGTA134900.017.74620210-11
GCTTATA27700.017.4921671
TATAAGC29100.017.4648763
TTATAGG26850.017.1594033
CCTATAA29750.017.0851971
Produced by FastQC (version 0.11.5)