FastQCFastQC Report
Fri 27 May 2016
SRR940225_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940225_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5378565
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA79300.042.2416531
GGTATCA44100.037.709821
TCAACGC89550.036.0160374
CAACGCA91900.035.0940865
ATCAACG92350.035.026923
AACGCAG96400.033.5544176
TATCAAC98300.033.3899882
ACGCAGA114450.028.2210247
CGCAGAG120000.026.7574718
GTACATG148100.026.1473431
TACATGG157450.024.1345422
ACATGGG157200.023.508173
GCAGAGT146550.021.8450839
CATGGGG132200.021.414434
AGAGTAC131800.018.66766210-11
GCTTATA28600.018.274761
GAGTACA113250.017.8311
TTATAGG28800.017.8124223
AGTACAT108500.017.7741172
CTTATAG31500.017.642782
Produced by FastQC (version 0.11.5)