FastQCFastQC Report
Fri 27 May 2016
SRR940224_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940224_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5444286
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA76450.042.369561
GGTATCA40750.041.609041
TCAACGC87300.036.072674
ATCAACG89600.035.1997073
CAACGCA89700.035.0548865
AACGCAG95250.033.063096
TATCAAC96550.032.8632972
ACGCAGA113150.027.8745887
GTACATG156800.027.4731391
CGCAGAG121150.026.0339228
TACATGG164500.025.958572
ACATGGG165150.024.906823
CATGGGG141450.021.9275054
GCAGAGT148300.020.853879
AGAGTAC132300.018.40360310-11
TATAAGC26750.018.1115423
CAGAGTA137650.016.77369910-11
GCTTATA28200.016.6737441
ATGGGGA88450.016.5937165
GAGTACA115300.016.1474461
Produced by FastQC (version 0.11.5)