##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940224_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5444286 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.432462401865 34.0 31.0 34.0 31.0 34.0 2 32.43652611196399 34.0 31.0 34.0 31.0 34.0 3 32.54560855179173 34.0 31.0 34.0 31.0 34.0 4 35.91596528911229 37.0 37.0 37.0 35.0 37.0 5 35.92223626752893 37.0 37.0 37.0 35.0 37.0 6 35.995171267637296 37.0 37.0 37.0 35.0 37.0 7 35.95650118307525 37.0 37.0 37.0 35.0 37.0 8 35.94491251929087 37.0 37.0 37.0 35.0 37.0 9 37.72053139750557 39.0 39.0 39.0 35.0 39.0 10-11 37.65514164759162 39.0 39.0 39.0 35.0 39.0 12-13 37.59938924957285 39.0 38.0 39.0 35.0 39.0 14-15 39.07416105619727 41.0 39.5 41.0 36.0 41.0 16-17 38.989946799268075 41.0 39.0 41.0 36.0 41.0 18-19 38.94190275823129 41.0 39.0 41.0 36.0 41.0 20-21 38.872892331519694 41.0 39.0 41.0 36.0 41.0 22-23 38.76659381964871 41.0 39.0 41.0 35.5 41.0 24-25 38.65022502491603 40.5 39.0 41.0 35.0 41.0 26-27 38.500305641547854 40.0 38.5 41.0 35.0 41.0 28-29 38.34401508296956 40.0 38.0 41.0 34.0 41.0 30-31 38.3133175773646 40.0 38.0 41.0 34.5 41.0 32-33 38.333954075888 40.0 38.0 41.0 34.0 41.0 34-35 38.3676683223475 40.0 38.5 41.0 34.5 41.0 36-37 38.51083383569489 41.0 39.0 41.0 35.0 41.0 38-39 38.441691619433655 41.0 39.0 41.0 35.0 41.0 40-41 38.33039208447168 41.0 38.5 41.0 34.5 41.0 42-43 38.21511957674524 40.0 38.0 41.0 34.0 41.0 44-45 38.06583067090891 40.0 38.0 41.0 33.5 41.0 46-47 37.89572893488696 40.0 38.0 41.0 33.0 41.0 48-49 37.7126521273864 40.0 38.0 41.0 33.0 41.0 50-51 36.77280354118061 39.0 36.5 40.5 31.5 40.5 52-53 36.8492732564013 39.5 37.0 40.5 32.0 41.0 54-55 37.1023775202111 40.0 37.0 41.0 32.0 41.0 56-57 37.02976046813117 40.0 36.0 41.0 32.0 41.0 58-59 36.79078413588118 39.0 36.0 41.0 32.0 41.0 60-61 36.47738463776517 39.0 35.0 41.0 31.0 41.0 62-63 36.127017206664014 38.5 35.0 41.0 31.0 41.0 64-65 35.73312607015869 37.5 35.0 40.0 30.5 41.0 66-67 35.34157362416302 37.0 35.0 40.0 30.0 41.0 68-69 34.93219221032841 36.5 35.0 39.0 30.0 41.0 70-71 34.517422027424715 36.0 34.5 39.0 29.0 40.5 72-73 34.10150274985554 35.5 34.0 38.5 29.0 40.0 74-75 33.68190218147981 35.0 34.0 37.0 29.0 39.0 76-77 33.268445210262655 35.0 34.0 37.0 28.0 39.0 78-79 32.88354092712984 35.0 34.0 36.0 27.5 38.5 80-81 32.50753138244391 35.0 33.0 36.0 26.5 37.0 82-83 32.18023878613283 35.0 33.0 35.0 26.0 37.0 84-85 31.87257153646961 35.0 33.0 35.0 25.5 36.5 86-87 31.612904428606434 35.0 33.0 35.0 25.0 36.0 88-89 31.341849785261097 35.0 32.5 35.0 24.5 36.0 90-91 31.093995704854596 35.0 32.0 35.0 23.5 35.0 92-93 30.79956637105398 34.0 32.0 35.0 21.5 35.0 94-95 30.48600523558094 34.0 31.5 35.0 18.5 35.0 96-97 30.159190479706616 34.0 31.0 35.0 12.5 35.0 98-99 29.743542863104548 34.0 31.0 35.0 2.0 35.0 100-101 27.817546965754556 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.15110249422919964 1101 2 0.19799224972990714 1101 3 0.1908048678655021 1101 4 0.15205088985805304 1101 5 0.17011936138379724 1101 6 0.07712289382578064 1101 7 0.11295682171793686 1101 8 0.15930064160081514 1101 9 0.15969516389344562 1101 10-11 0.15924275925610232 1101 12-13 0.16389743186741867 1101 14-15 0.18312102628258486 1101 16-17 0.16717080354210623 1101 18-19 0.17439185903016607 1101 20-21 0.12787103269743483 1101 22-23 0.2026712513553477 1101 24-25 0.16616458737288298 1101 26-27 0.1622723040978471 1101 28-29 0.13944056174008068 1101 30-31 0.14591751198891956 1101 32-33 0.08094640852498713 1101 34-35 0.05206487731860676 1101 36-37 0.056166414399207554 1101 38-39 0.1321418459761503 1101 40-41 0.1433640158616427 1101 42-43 0.1612609460279586 1101 44-45 0.17175439908544377 1101 46-47 0.17413881222949357 1101 48-49 0.1600116228103019 1101 50-51 0.12171306388834324 1101 52-53 0.17038333592918775 1101 54-55 0.403338903216671 1101 56-57 0.2515783980706985 1101 58-59 0.2392427994476236 1101 60-61 0.2390864096331029 1101 62-63 0.2270744686402537 1101 64-65 0.2668776871685381 1101 66-67 0.2556833365402511 1101 68-69 0.2997659315925105 1101 70-71 0.2891507822551702 1101 72-73 0.20194006669932918 1101 74-75 0.2782940203696995 1101 76-77 0.28060878307331905 1101 78-79 0.3491885080182726 1101 80-81 0.24688632617348816 1101 82-83 0.18575646125066925 1101 84-85 0.20030090502322295 1101 86-87 0.1747122841413038 1101 88-89 0.2263717501003022 1101 90-91 0.21543737377006877 1101 92-93 0.2635697323745738 1101 94-95 0.3074792063221352 1101 96-97 0.21560526508726596 1101 98-99 0.167125330863513 1101 100-101 0.3623497769714952 1102 1 0.09671411077448511 1102 2 0.11750759574193381 1102 3 0.14142426141199138 1102 4 0.11289810597397576 1102 5 0.08851218672049299 1102 6 0.06397325896051598 1102 7 0.11851177674509472 1102 8 0.15906783781468903 1102 9 0.14361683600788666 1102 10-11 0.16329875024227647 1102 12-13 0.14108020087913786 1102 14-15 0.17152529429970542 1102 16-17 0.15236034717966618 1102 18-19 0.12784532672277749 1102 20-21 0.14705743373344404 1102 22-23 0.18142282554388345 1102 24-25 0.23638687256343616 1102 26-27 0.1367304666549316 1102 28-29 0.13860050728181506 1102 30-31 0.18186487651458805 1102 32-33 0.0901882197142001 1102 34-35 0.08158133137682455 1102 36-37 0.1820092140169649 1102 38-39 0.1630868525167628 1102 40-41 0.1446986446725802 1102 42-43 0.11858548078046738 1102 44-45 0.09450370341924952 1102 46-47 0.08806340918659572 1102 48-49 0.1623217993090833 1102 50-51 0.12711885336555895 1102 52-53 0.1647739768319454 1102 54-55 0.3703430129700038 1102 56-57 0.2687950223858522 1102 58-59 0.25138545989649685 1102 60-61 0.19063086845378763 1102 62-63 0.16064264264429085 1102 64-65 0.1852426330931749 1102 66-67 0.15977200918504053 1102 68-69 0.20424981807666853 1102 70-71 0.08647392731524661 1102 72-73 0.09839370209622444 1102 74-75 0.08747153065407076 1102 76-77 0.0466850788278208 1102 78-79 0.1645603459332179 1102 80-81 0.19919474149201477 1102 82-83 0.1766860442730689 1102 84-85 0.19135016986951214 1102 86-87 0.22450288194863077 1102 88-89 0.09274560331883563 1102 90-91 0.09982940222617742 1102 92-93 0.164766432963809 1102 94-95 0.12052587631531253 1102 96-97 0.10069770138138523 1102 98-99 0.1846076652273645 1102 100-101 0.5300322833748474 1103 1 0.04160875765447969 1103 2 0.12432376907675291 1103 3 0.0750230454067804 1103 4 0.10430792998727156 1103 5 0.08274259657120808 1103 6 0.13307708146373187 1103 7 0.04321587004974248 1103 8 0.1140644870234766 1103 9 0.0658560612803143 1103 10-11 0.06931956416623564 1103 12-13 0.09654332540467436 1103 14-15 0.0677873651913572 1103 16-17 0.06479181682505697 1103 18-19 -1.1707733131061104E-4 1103 20-21 0.05993427795211659 1103 22-23 0.02496391021450961 1103 24-25 -0.017884273872645906 1103 26-27 -0.03689098566491822 1103 28-29 0.006001415931514487 1103 30-31 0.07095187318569884 1103 32-33 -0.03459124072285391 1103 34-35 -0.10751480381578915 1103 36-37 -0.01104189872637562 1103 38-39 0.003904144379227148 1103 40-41 0.04845582628409062 1103 42-43 0.002772142098642405 1103 44-45 0.05623316690247293 1103 46-47 0.015840852303576014 1103 48-49 0.04319316246845517 1103 50-51 0.03966104629583356 1103 52-53 0.03865693804149117 1103 54-55 0.09448068907478557 1103 56-57 0.08076181361479229 1103 58-59 0.12142993403448088 1103 60-61 0.12752107750345232 1103 62-63 0.12479865740009188 1103 64-65 0.11750236698764382 1103 66-67 0.06203656561763893 1103 68-69 0.10721668871148182 1103 70-71 0.03821745111544317 1103 72-73 0.10162879004850822 1103 74-75 0.10919872490892146 1103 76-77 0.06966332640850226 1103 78-79 0.05717005439726819 1103 80-81 0.04300649068718343 1103 82-83 0.08070105540700467 1103 84-85 0.06314210387501262 1103 86-87 -0.06133066416051847 1103 88-89 -0.0285042144241352 1103 90-91 -0.021525542740754133 1103 92-93 -0.004165554531134319 1103 94-95 -0.00746769112389245 1103 96-97 -0.19574426489986863 1103 98-99 -0.2286752784581232 1103 100-101 -0.006977667687852573 1104 1 0.04095105804474741 1104 2 0.10021727703701089 1104 3 0.10376259133432342 1104 4 0.04914176239033452 1104 5 0.10438954342070161 1104 6 0.10576336126338504 1104 7 0.1142400549630338 1104 8 0.07341194847055021 1104 9 0.1588457374910277 1104 10-11 0.17107147484644258 1104 12-13 0.1264353118585788 1104 14-15 0.11260980305340951 1104 16-17 0.1131293868051344 1104 18-19 0.21866673973939044 1104 20-21 0.20268726608670562 1104 22-23 0.1709421399385107 1104 24-25 0.18673939627550595 1104 26-27 0.22176735740125508 1104 28-29 0.22237444974481235 1104 30-31 0.28514360644619785 1104 32-33 0.11985120595321774 1104 34-35 0.17656367105841042 1104 36-37 0.18360987237070248 1104 38-39 0.14002173939378082 1104 40-41 0.17287340605006563 1104 42-43 0.22990924691334413 1104 44-45 0.23481670815824884 1104 46-47 0.16446630201092916 1104 48-49 0.24993370374266277 1104 50-51 0.06598302251140353 1104 52-53 0.11806021598283678 1104 54-55 0.31418545316589785 1104 56-57 0.274451751397244 1104 58-59 0.2050584921055787 1104 60-61 0.24947232562461608 1104 62-63 0.14881726066396084 1104 64-65 0.2031315048398099 1104 66-67 0.20291598959007473 1104 68-69 0.20064061423139634 1104 70-71 0.1816066903119946 1104 72-73 0.12059801976078433 1104 74-75 0.15671329065123274 1104 76-77 0.12434371791152898 1104 78-79 0.01778214232961517 1104 80-81 0.09472539071180819 1104 82-83 0.1135414631304883 1104 84-85 0.04130446603038607 1104 86-87 0.06376422052840525 1104 88-89 0.13391022178905843 1104 90-91 0.11003013048391708 1104 92-93 0.19413925614353644 1104 94-95 0.1360912932203462 1104 96-97 0.07597632754923822 1104 98-99 0.1568063168446443 1104 100-101 0.32621923835912625 1105 1 -0.14473308819698616 1105 2 -0.01467050124198721 1105 3 -0.04783321789403061 1105 4 0.014029606620042046 1105 5 0.07128736731121421 1105 6 0.07386425792918061 1105 7 0.10779864952711193 1105 8 0.06010975706049493 1105 9 0.08547082883275436 1105 10-11 0.05141892294983563 1105 12-13 0.05267386611357949 1105 14-15 0.024905059087238612 1105 16-17 0.04649190332578712 1105 18-19 0.13515860406032232 1105 20-21 0.052201440212392924 1105 22-23 0.1160242947327319 1105 24-25 0.1625683636721149 1105 26-27 0.21124683486061713 1105 28-29 0.2164557696031011 1105 30-31 0.19346223277213426 1105 32-33 0.20380121136116713 1105 34-35 0.1800504189235852 1105 36-37 0.2140701951070767 1105 38-39 0.21383835290418318 1105 40-41 0.11642372380057253 1105 42-43 0.09519826928646324 1105 44-45 0.0557440542226999 1105 46-47 0.014732479059553327 1105 48-49 0.10317389489419071 1105 50-51 0.025277059776193767 1105 52-53 0.1528271857589445 1105 54-55 0.39046878795014806 1105 56-57 0.29361742220825704 1105 58-59 0.25547744396421024 1105 60-61 0.29357478268411086 1105 62-63 0.35091670245719087 1105 64-65 0.3887425447241597 1105 66-67 0.43832119732160635 1105 68-69 0.43735220651885953 1105 70-71 0.4931499104475421 1105 72-73 0.429608468990601 1105 74-75 0.2975587061538647 1105 76-77 0.2919763975414682 1105 78-79 0.38397119398484136 1105 80-81 0.4664409796120381 1105 82-83 0.4991978771004284 1105 84-85 0.5665305450498899 1105 86-87 0.5811169394121976 1105 88-89 0.6585114178710576 1105 90-91 0.532638098384389 1105 92-93 0.5764108160887709 1105 94-95 0.5379185564507125 1105 96-97 0.371785028293953 1105 98-99 0.2853158836540288 1105 100-101 0.4582790635633778 1106 1 0.016508908875337625 1106 2 0.08655826397594524 1106 3 0.037206581856963794 1106 4 0.14270131597766778 1106 5 0.1256396836671385 1106 6 0.16599508137769448 1106 7 0.17790533786484986 1106 8 0.15111144490333572 1106 9 0.06568217384554487 1106 10-11 0.052677577051440494 1106 12-13 0.18665498727882124 1106 14-15 0.17825899556391533 1106 16-17 0.055309905419960614 1106 18-19 0.24192306402875374 1106 20-21 0.21896558761734042 1106 22-23 0.27968807279182073 1106 24-25 0.29911061704649455 1106 26-27 0.3221727521042297 1106 28-29 0.2880127525319267 1106 30-31 0.2856443687268353 1106 32-33 0.29104673258683533 1106 34-35 0.06215772196951974 1106 36-37 0.20569505640702346 1106 38-39 0.15478110950578383 1106 40-41 0.22589810922260511 1106 42-43 0.2681138615161345 1106 44-45 0.27626827528844444 1106 46-47 0.1698529201908272 1106 48-49 0.09414400080112273 1106 50-51 0.017680334399685194 1106 52-53 0.1739885154040266 1106 54-55 0.2885147452018799 1106 56-57 0.26040846309677335 1106 58-59 0.26475843072714156 1106 60-61 0.31538188152189406 1106 62-63 0.3138473888987079 1106 64-65 0.2074634698078981 1106 66-67 0.359592380009758 1106 68-69 0.3161812394995209 1106 70-71 0.30249334479097456 1106 72-73 0.23571114088750278 1106 74-75 0.300286538890731 1106 76-77 0.13538111256170282 1106 78-79 0.07987270522931311 1106 80-81 0.2994048368538742 1106 82-83 0.4095716639633906 1106 84-85 0.33214439369622895 1106 86-87 0.3507074421705738 1106 88-89 0.391226135964839 1106 90-91 0.24648661448922482 1106 92-93 0.40533290182493786 1106 94-95 0.4280347128574853 1106 96-97 0.3102002532615238 1106 98-99 0.5168682562713869 1106 100-101 0.8200668746291662 1107 1 0.1046408926021627 1107 2 0.10056176781100845 1107 3 0.1364809329061103 1107 4 0.1603576601400576 1107 5 0.0977977495196356 1107 6 0.07614637119754519 1107 7 0.08653803400419946 1107 8 0.04903685763487431 1107 9 0.1225394068849397 1107 10-11 0.10000389826008416 1107 12-13 0.13237384619696257 1107 14-15 0.1259572253826633 1107 16-17 0.151049748530113 1107 18-19 0.17388198846721536 1107 20-21 0.18309930798890406 1107 22-23 0.23452616201169718 1107 24-25 0.23139055666802477 1107 26-27 0.17525106466336382 1107 28-29 0.23977176555262503 1107 30-31 0.16250924134848788 1107 32-33 0.16670072664698665 1107 34-35 0.2383835233301994 1107 36-37 0.15280969023208257 1107 38-39 0.18474658343033923 1107 40-41 0.21132658446889963 1107 42-43 0.21341803207258891 1107 44-45 0.24449604493133137 1107 46-47 0.21934756548321133 1107 48-49 0.16406778316115833 1107 50-51 0.1437142075628941 1107 52-53 0.2500351954471256 1107 54-55 0.3601266493319599 1107 56-57 0.34013666769630646 1107 58-59 0.34433034366453796 1107 60-61 0.370555160993149 1107 62-63 0.3460892578822552 1107 64-65 0.3781618854457207 1107 66-67 0.3478415824831913 1107 68-69 0.33956634762777327 1107 70-71 0.3291326890532673 1107 72-73 0.37153357485014027 1107 74-75 0.3416943952194913 1107 76-77 0.39617110773168207 1107 78-79 0.4607835496179291 1107 80-81 0.5092115500134966 1107 82-83 0.5886906269911236 1107 84-85 0.5572720806750802 1107 86-87 0.6012540493496772 1107 88-89 0.6418138842512597 1107 90-91 0.6753764855040565 1107 92-93 0.6918278003912661 1107 94-95 0.746564231681532 1107 96-97 0.8607976902309957 1107 98-99 0.8661056503242825 1107 100-101 1.0858007868016522 1108 1 0.25365508280923876 1108 2 0.3326956894693751 1108 3 0.28100777094486773 1108 4 0.2482378462607926 1108 5 0.2683605557655113 1108 6 0.24283675708345953 1108 7 0.28625761684943285 1108 8 0.24257986739311832 1108 9 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-1.294685907034502 2316 98-99 -1.1601068992191301 2316 100-101 -1.244180977008707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 33296.0 3 8918.0 4 6811.0 5 5695.0 6 5158.0 7 4982.0 8 5688.0 9 6248.0 10 7229.0 11 8436.0 12 9456.0 13 10384.0 14 11184.0 15 12788.0 16 15198.0 17 18809.0 18 18010.0 19 14756.0 20 15112.0 21 16601.0 22 17332.0 23 19056.0 24 21780.0 25 25640.0 26 30252.0 27 36044.0 28 43758.0 29 53380.0 30 66453.0 31 84366.0 32 109734.0 33 148222.0 34 217764.0 35 349232.0 36 674959.0 37 1456972.0 38 1616798.0 39 237767.0 40 18.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.80687437339554 17.73987158248526 13.42686707231105 25.02638697180815 2 15.890032548296018 19.615686095810396 38.46389461657725 26.030386739316334 3 18.73988021518569 23.64837889613438 28.74170460121916 28.87003628746077 4 13.496106553160466 16.1859506683089 35.75621643390253 34.5617263446281 5 15.340835692023406 36.06729447054934 33.583857059614665 15.00801277781259 6 33.89862177184176 34.3397018153398 17.517903260387694 14.243773152430744 7 30.166755591223364 30.63601635623912 20.85951463360143 18.33771341893608 8 27.38511265014967 33.63879920691943 19.933738448788787 19.042349694142118 9 27.10517656444033 15.871517476942715 19.260436201061317 37.76286975755564 10-11 25.84441523933481 25.27635482995577 27.249371684913328 21.62985824579609 12-13 26.539477091628434 22.319745743334853 27.36889603629277 23.771881128743942 14-15 23.79605309759067 23.6851169221353 24.6235496875091 27.89528029276493 16-17 24.367697366855477 26.81143248181638 25.113234485173464 23.707635666154683 18-19 24.390780396112415 26.056577565047405 26.250225337427292 23.302416701412888 20-21 24.799244200490097 25.493680478278574 26.071426471431323 23.635648849800006 22-23 24.77232961462133 25.141471940322724 25.956743936086628 24.129454508969314 24-25 24.65858130464417 25.439725380614604 25.786131978240622 24.115561336500605 26-27 24.450087104909144 25.705933753234973 25.73962926122323 24.104349880632654 28-29 24.352762277331678 25.637219835618463 25.88012948199394 24.129888405055926 30-31 24.482529021532972 25.432707138590672 25.764220381206698 24.320543458669658 32-33 24.335464372423292 25.46594162460846 25.80506550680517 24.393528496163082 34-35 24.727184358085978 25.508241846585243 25.51964381036908 24.244929984959697 36-37 24.57895714506459 25.366602132760768 25.589968943249175 24.46447177892547 38-39 24.45711425100638 25.51220173099931 25.86396797949806 24.16671603849625 40-41 24.60231428710788 25.274933314496632 25.843681041623018 24.279071356772473 42-43 24.5913462002171 25.28983925014695 25.666340231702378 24.45247431793357 44-45 24.524357971906998 25.46124756203491 25.538318123762643 24.476076342295443 46-47 24.562235673106944 25.451877682536484 25.450977514067198 24.53490913028937 48-49 24.66867493122159 25.5579346231252 25.365406528427613 24.407983917225597 50-51 24.426174936200834 25.554608613636 25.491052868569586 24.528163581593578 52-53 24.772154615168944 25.408600396198523 25.29208709018238 24.527157898450152 54-55 24.583736643457254 25.2617845174292 25.506411609011497 24.648067230102047 56-57 24.517649250529903 25.63917663470684 25.58107426098833 24.262099853774927 58-59 24.66433020919721 25.295599961638786 25.542017469049227 24.498052360114777 60-61 24.6998969810345 25.250421514291528 25.69073458539115 24.358946919282825 62-63 24.719969053913047 25.385271341827213 25.577673915805292 24.31708568845444 64-65 24.504033775949754 25.33101617570519 25.511113666814683 24.653836381530372 66-67 24.445793316506318 25.595092326597936 25.401429775807788 24.557684581087962 68-69 24.281229899689748 25.37539410616877 25.72301407327155 24.62036192086993 70-71 24.296813165359392 25.374186065206654 25.594781630051568 24.734219139382386 72-73 24.53397280000676 25.34848808597084 25.461838232740856 24.655700881281543 74-75 24.34968988665773 25.428402909396926 25.496856605538216 24.725050598407126 76-77 24.542449991882417 25.301937754547293 25.5480102828217 24.60760197074859 78-79 24.375647704330536 25.43138566767482 25.53823362893286 24.654732999061785 80-81 24.312004013227632 25.47848378849129 25.652946384994816 24.55656581328626 82-83 24.46197646771372 25.45112428003044 25.486185264741668 24.600713987514176 84-85 24.384344049826666 25.383798800819662 25.442026066301725 24.789831083051943 86-87 24.488059231780664 25.241610616188137 25.694711471775854 24.575618680255346 88-89 24.343424896818597 25.23505773620287 25.61670444579878 24.804812921179753 90-91 24.32370834908775 25.453855817780095 25.714635054211733 24.507800778920423 92-93 24.46473472660169 25.2453437946219 25.746252989187884 24.543668489588523 94-95 24.368479086155954 25.20211564388824 25.629724085251105 24.799681184704696 96-97 24.571050143233023 25.148505094374208 25.547877115791962 24.732567646600806 98-99 24.222952287767797 25.071976689533653 25.64699980865036 25.058071214048187 100-101 24.198890701036355 25.002987224605466 25.91178367273107 24.88633840162711 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 296.0 1 223.0 2 179.5 3 208.5 4 171.0 5 167.0 6 264.5 7 540.0 8 917.0 9 1100.5 10 1042.5 11 1022.5 12 1119.0 13 1220.5 14 1310.5 15 1386.5 16 1426.0 17 1442.0 18 1551.0 19 1691.0 20 1882.5 21 2193.5 22 2578.0 23 3108.5 24 3779.0 25 4804.0 26 6559.0 27 9229.5 28 12646.0 29 17177.5 30 23524.0 31 31028.5 32 38741.5 33 47119.0 34 57225.0 35 66813.5 36 77063.5 37 89059.0 38 101147.0 39 109565.0 40 116377.0 41 129582.5 42 144833.5 43 160731.0 44 175621.5 45 186706.5 46 197555.5 47 211740.0 48 231712.5 49 247375.5 50 254647.5 51 257414.0 52 254198.5 53 253705.5 54 253138.5 55 243777.0 56 231203.5 57 213870.5 58 192470.5 59 169771.0 60 142404.5 61 112031.5 62 87285.0 63 68191.0 64 51552.5 65 38082.0 66 27317.0 67 20257.5 68 14431.0 69 9743.0 70 6972.0 71 4975.5 72 3542.5 73 2524.0 74 1828.5 75 1345.0 76 872.5 77 513.0 78 341.5 79 236.0 80 170.0 81 140.5 82 114.5 83 94.0 84 73.5 85 59.5 86 59.5 87 53.0 88 38.0 89 35.5 90 34.0 91 31.0 92 25.0 93 20.5 94 21.0 95 16.5 96 15.0 97 13.5 98 9.5 99 7.5 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02312516278534963 2 0.03511938939284233 3 0.02496195093351084 4 0.023768038637206057 5 0.043219625126233265 6 0.02356599194090832 7 0.02700078577796978 8 0.04070322536325241 9 0.026247702637223685 10-11 0.028562055703906814 12-13 0.026440565392780616 14-15 0.036138806815071804 16-17 0.04354106305216148 18-19 0.044946205985504806 20-21 0.03860928687434863 22-23 0.04559826577810203 24-25 0.020498555733479102 26-27 0.026155863229815626 28-29 0.027946731674272807 30-31 0.02786407620760555 32-33 0.03723169576322772 34-35 0.04116242240029271 36-37 0.030655994192810593 38-39 0.01650354151122847 40-41 0.018579112118650636 42-43 0.020930200948296986 44-45 0.022445551170529984 46-47 0.01580556201492721 48-49 0.026523220859447867 50-51 0.018974021570505296 52-53 0.028653895111314873 54-55 0.024016005037207817 56-57 0.024006821096467013 58-59 0.02365783134831638 60-61 0.02045263602977507 62-63 0.02335476130386978 64-65 0.020627130903850387 66-67 0.019378114963100764 68-69 0.0233823131260922 70-71 0.036249014103961474 72-73 0.02602728805944434 74-75 0.03528470032617684 76-77 0.04398189220772017 78-79 0.027652845570567016 80-81 0.023611911644612352 82-83 0.01677905973345265 84-85 0.033401992474311595 86-87 0.020700602429776834 88-89 0.03098661605947961 90-91 0.02512726186684535 92-93 0.043743109748459214 94-95 0.07614405268202296 96-97 0.09637627413401868 98-99 0.07315927194126098 100-101 0.08181054411910028 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 5444286.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.33567657978496 #Duplication Level Percentage of deduplicated Percentage of total 1 76.99273014915626 37.214957034842755 2 11.345695919464147 10.968037770716098 3 4.005575264232815 5.808365714638321 4 2.089555441011095 4.040003039689688 5 1.2599165916488133 3.0449460445721015 6 0.8106782635098478 2.3510809413164213 7 0.5538435344589784 1.8739281370189913 8 0.4519714368712348 1.7477076156726963 9 0.35431595284072853 1.5413491173211014 >10 1.9124787943330785 16.8419395209306 >50 0.1402731534334514 4.67107469146149 >100 0.07658989666956802 6.939058256283399 >500 0.004390390169615661 1.4047348427963213 >1k 0.001985212200490687 1.5528172727398744 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.8367881481612097E-5 0.0 0.0 0.0 0.0 3 1.8367881481612097E-5 1.8367881481612097E-5 0.0 0.0 0.0 4 1.8367881481612097E-5 1.8367881481612097E-5 0.0 1.8367881481612097E-5 0.0 5 1.8367881481612097E-5 5.510364444483629E-5 0.0 1.8367881481612097E-5 0.0 6 1.8367881481612097E-5 5.510364444483629E-5 0.0 1.8367881481612097E-5 0.0 7 3.6735762963224195E-5 5.510364444483629E-5 0.0 1.8367881481612097E-5 0.0 8 3.6735762963224195E-5 5.510364444483629E-5 0.0 1.8367881481612097E-5 1.8367881481612097E-5 9 3.6735762963224195E-5 5.510364444483629E-5 0.0 3.6735762963224195E-5 1.8367881481612097E-5 10-11 5.510364444483629E-5 7.347152592644839E-5 0.0 3.6735762963224195E-5 1.8367881481612097E-5 12-13 5.510364444483629E-5 7.347152592644839E-5 0.0 5.510364444483629E-5 5.510364444483629E-5 14-15 7.347152592644839E-5 7.347152592644839E-5 0.0 5.510364444483629E-5 1.0102334814886653E-4 16-17 7.347152592644839E-5 7.347152592644839E-5 0.0 5.510364444483629E-5 1.1939122963047864E-4 18-19 9.18394074080605E-5 7.347152592644839E-5 0.0 6.428758518564234E-5 1.7449487407531492E-4 20-21 1.1020728888967258E-4 7.347152592644839E-5 0.0 7.347152592644839E-5 2.0204669629773306E-4 22-23 1.1020728888967258E-4 7.347152592644839E-5 0.0 8.265546666725444E-5 2.2041457777934517E-4 24-25 1.1020728888967258E-4 7.347152592644839E-5 0.0 9.18394074080605E-5 2.2041457777934517E-4 26-27 1.1020728888967258E-4 7.347152592644839E-5 0.0 1.4694305185289678E-4 2.2041457777934517E-4 28-29 1.377591111120907E-4 7.347152592644839E-5 0.0 6.520597925972295E-4 2.3878245926095727E-4 30-31 1.4694305185289678E-4 7.347152592644839E-5 0.0 0.0023602727703871543 2.3878245926095727E-4 32-33 1.4694305185289678E-4 7.347152592644839E-5 0.0 0.005813434488930229 2.3878245926095727E-4 34-35 1.4694305185289678E-4 7.347152592644839E-5 0.0 0.010056415111182625 2.5715034074256935E-4 36-37 1.6531093333450888E-4 7.347152592644839E-5 0.0 0.01773418957049648 2.5715034074256935E-4 38-39 1.83678814816121E-4 7.347152592644839E-5 0.0 0.030720281777996232 2.5715034074256935E-4 40-41 1.83678814816121E-4 7.347152592644839E-5 0.0 0.04541458696328591 2.5715034074256935E-4 42-43 2.5715034074256935E-4 9.18394074080605E-5 0.0 0.06144974749673327 2.663342814833754E-4 44-45 3.1225398518740563E-4 9.18394074080605E-5 0.0 0.07995538808945746 2.9388610370579356E-4 46-47 3.398058074098238E-4 9.18394074080605E-5 0.0 0.10230909985257938 3.398058074098238E-4 48-49 4.0409339259546613E-4 9.18394074080605E-5 0.0 0.12717921137868216 3.8572551111385405E-4 50-51 4.0409339259546613E-4 9.18394074080605E-5 0.0 0.15377590376405648 3.8572551111385405E-4 52-53 4.0409339259546613E-4 9.18394074080605E-5 0.0 0.18243898281611215 4.132773333362722E-4 54-55 4.0409339259546613E-4 9.18394074080605E-5 0.0 0.2154460658385691 4.591970370403024E-4 56-57 5.143006814851387E-4 9.18394074080605E-5 0.0 0.25436760669810515 4.7756491852191454E-4 58-59 5.326685629667508E-4 9.18394074080605E-5 0.0 0.2980556128021195 4.7756491852191454E-4 60-61 5.326685629667508E-4 9.18394074080605E-5 0.0 0.34571108130616207 4.7756491852191454E-4 62-63 5.326685629667508E-4 9.18394074080605E-5 0.0 0.39718706915838 5.143006814851387E-4 64-65 5.326685629667508E-4 9.18394074080605E-5 0.0 0.4552020228180518 5.143006814851387E-4 66-67 5.602203851891689E-4 9.18394074080605E-5 0.0 0.5228233784926067 5.326685629667508E-4 68-69 5.877722074115871E-4 9.18394074080605E-5 0.0 0.5992888691005579 5.326685629667508E-4 70-71 5.969561481523932E-4 1.0102334814886653E-4 0.0 0.6852413704937617 5.602203851891689E-4 72-73 6.061400888931992E-4 1.1020728888967258E-4 0.0 0.7800196389388802 5.78588266670781E-4 74-75 6.061400888931992E-4 1.1939122963047864E-4 0.0 0.8853502552951846 6.061400888931992E-4 76-77 6.061400888931992E-4 1.2857517037128467E-4 0.0 1.0083875093997634 6.245079703748113E-4 78-79 6.061400888931992E-4 1.2857517037128467E-4 0.0 1.1479650407785336 6.245079703748113E-4 80-81 6.979794963012597E-4 1.2857517037128467E-4 0.0 1.3021909576388895 6.428758518564234E-4 82-83 7.163473777828718E-4 1.2857517037128467E-4 0.0 1.470844845403052 6.796116148196477E-4 84-85 7.34715259264484E-4 1.2857517037128467E-4 0.0 1.6563145286636303 6.796116148196477E-4 86-87 7.53083140746096E-4 1.2857517037128467E-4 0.0 1.8644226258502952 6.796116148196477E-4 88-89 7.53083140746096E-4 1.2857517037128467E-4 0.0 2.1002478561927127 6.796116148196477E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7645 0.0 42.36956 1 GGTATCA 4075 0.0 41.60904 1 TCAACGC 8730 0.0 36.07267 4 ATCAACG 8960 0.0 35.199707 3 CAACGCA 8970 0.0 35.054886 5 AACGCAG 9525 0.0 33.06309 6 TATCAAC 9655 0.0 32.863297 2 ACGCAGA 11315 0.0 27.874588 7 GTACATG 15680 0.0 27.473139 1 CGCAGAG 12115 0.0 26.033922 8 TACATGG 16450 0.0 25.95857 2 ACATGGG 16515 0.0 24.90682 3 CATGGGG 14145 0.0 21.927505 4 GCAGAGT 14830 0.0 20.85387 9 AGAGTAC 13230 0.0 18.403603 10-11 TATAAGC 2675 0.0 18.111542 3 CAGAGTA 13765 0.0 16.773699 10-11 GCTTATA 2820 0.0 16.673744 1 ATGGGGA 8845 0.0 16.593716 5 GAGTACA 11530 0.0 16.147446 1 >>END_MODULE