FastQCFastQC Report
Fri 27 May 2016
SRR940224_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940224_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5444286
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45600.044.6139871
GTATCAA81950.039.1513671
TCAACGC92900.033.4902534
ATCAACG94300.033.0428123
CAACGCA96950.032.237625
TATCAAC101800.030.7486822
AACGCAG101850.030.7333056
GTACATG153350.026.5017431
ACGCAGA118800.026.268177
TACATGG162300.024.5253032
CGCAGAG128950.024.2005338
ACATGGG161000.023.9265373
CATGGGG136050.020.9481454
GCTTATA28250.020.1908651
GCAGAGT156850.019.74449
TTATAGG28750.018.8344043
TATAGGA32200.018.1443794
AGAGTAC138250.017.84856410-11
CCTATAA26500.017.3987461
CAGAGTA145850.017.14647510-11
Produced by FastQC (version 0.11.5)