FastQCFastQC Report
Fri 27 May 2016
SRR940223_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940223_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3095815
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43150.13938171370059257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31500.10175026608502122No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28550.045.7708631
GTATCAA52250.040.7431141
TCAACGC58350.035.99344
ATCAACG58400.035.9637453
CAACGCA60550.034.6833885
AACGCAG63150.033.392936
TATCAAC62500.032.6172072
ACGCAGA73150.028.9582617
CGCAGAG77600.027.1135868
GTACATG79650.024.579551
TACATGG84200.022.9695152
ACATGGG84200.022.7430363
GCAGAGT93300.022.2958159
CATGGGG70850.020.1197624
GTGGTAT15900.019.1269341
CAGAGTA87650.018.77616110-11
GAGTACT49250.018.56495912-13
AGAGTAC84500.018.18331310-11
GTACTTT52150.017.89848714-15
CCTATAA16950.017.1010441
Produced by FastQC (version 0.11.5)