##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940223_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3095815 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.410560385552756 34.0 31.0 34.0 30.0 34.0 2 31.384486153080854 34.0 31.0 34.0 28.0 34.0 3 32.2366481847268 34.0 31.0 34.0 30.0 34.0 4 35.98885237005441 37.0 35.0 37.0 35.0 37.0 5 36.15134269974143 37.0 37.0 37.0 35.0 37.0 6 36.303437382401725 37.0 37.0 37.0 35.0 37.0 7 36.29255398013124 37.0 37.0 37.0 35.0 37.0 8 36.291056474627844 37.0 37.0 37.0 35.0 37.0 9 38.13094451703348 39.0 39.0 39.0 37.0 39.0 10-11 38.08399549068662 39.0 39.0 39.0 37.0 39.0 12-13 38.026594127879086 39.0 39.0 39.0 37.0 39.0 14-15 39.5571266370891 41.0 40.0 41.0 38.0 41.0 16-17 39.48877161587498 41.0 40.0 41.0 37.0 41.0 18-19 39.430839213583496 41.0 40.0 41.0 37.0 41.0 20-21 39.36064348160339 41.0 40.0 41.0 37.0 41.0 22-23 39.2687881866326 41.0 39.5 41.0 37.0 41.0 24-25 39.17082755267999 41.0 39.0 41.0 36.5 41.0 26-27 39.007126717843285 40.0 39.0 41.0 36.0 41.0 28-29 38.849415905020166 40.0 39.0 41.0 36.0 41.0 30-31 38.826291622722934 40.0 38.5 41.0 35.5 41.0 32-33 38.84859754216579 40.0 39.0 41.0 35.5 41.0 34-35 38.86591786007885 40.5 39.0 41.0 35.5 41.0 36-37 38.988950405628245 41.0 39.0 41.0 35.5 41.0 38-39 38.92262005320085 41.0 39.0 41.0 35.0 41.0 40-41 38.81227124359822 41.0 39.0 41.0 35.0 41.0 42-43 38.54442077449718 41.0 39.0 41.0 35.0 41.0 44-45 38.49508142443912 40.0 39.0 41.0 35.0 41.0 46-47 38.33114155723129 40.0 38.0 41.0 35.0 41.0 48-49 38.14693852830354 40.0 38.0 41.0 34.5 41.0 50-51 37.20485752540122 39.0 37.0 40.5 33.0 40.5 52-53 37.27313760673684 39.5 37.0 40.5 33.5 41.0 54-55 37.6272356390805 40.0 37.0 41.0 34.0 41.0 56-57 37.44722746675754 40.0 37.0 41.0 33.5 41.0 58-59 37.181074935033266 39.5 36.0 41.0 33.0 41.0 60-61 36.908120801792094 39.0 36.0 41.0 33.0 41.0 62-63 36.56663899490118 39.0 35.0 41.0 32.5 41.0 64-65 36.18414343234334 38.0 35.0 40.0 32.0 41.0 66-67 35.808280695067374 37.0 35.0 40.0 31.5 41.0 68-69 35.413198947611534 37.0 35.0 39.0 31.0 41.0 70-71 34.99773516828363 36.0 35.0 39.0 31.0 41.0 72-73 34.56638930297838 35.5 35.0 38.5 30.5 40.0 74-75 34.14318442801007 35.0 34.0 37.0 30.0 39.5 76-77 33.724765853256734 35.0 34.0 37.0 29.5 39.0 78-79 33.32773696102642 35.0 34.0 36.0 29.0 38.5 80-81 32.97401637371742 35.0 34.0 36.0 29.0 37.0 82-83 32.65583537775997 35.0 34.0 35.5 28.5 37.0 84-85 32.38289771837141 35.0 33.0 35.0 27.5 36.5 86-87 32.112580047580366 35.0 33.0 35.0 27.0 36.0 88-89 31.838376485675017 35.0 33.0 35.0 26.0 36.0 90-91 31.591704769180332 35.0 33.0 35.0 25.0 35.5 92-93 31.329059714485524 35.0 32.5 35.0 25.0 35.0 94-95 31.042384477108612 34.5 32.0 35.0 24.0 35.0 96-97 30.709162853723495 34.0 32.0 35.0 21.5 35.0 98-99 30.27369351850805 34.0 31.5 35.0 12.5 35.0 100-101 28.285617034609626 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.4754544896148829 1101 2 1.5687497770235446 1101 3 0.8125067375554664 1101 4 0.42292801181485373 1101 5 0.29962190251226417 1101 6 0.1987199851772985 1101 7 0.21603976008276504 1101 8 0.21867995343760782 1101 9 0.2106774614132334 1101 10-11 0.19643391191103632 1101 12-13 0.27267177140691246 1101 14-15 0.27669357343361867 1101 16-17 0.28030475977196545 1101 18-19 0.276929237508341 1101 20-21 0.3578419178995276 1101 22-23 0.25705064612671435 1101 24-25 0.24160292863872712 1101 26-27 0.34750858938072327 1101 28-29 0.39240101913505043 1101 30-31 0.30127460738391676 1101 32-33 0.315570524135417 1101 34-35 0.30467229904161997 1101 36-37 0.3567637744216796 1101 38-39 0.3760373127299559 1101 40-41 0.31472139650010433 1101 42-43 0.5071247840029969 1101 44-45 0.4207166618976146 1101 46-47 0.4763405840464756 1101 48-49 0.4498564119187307 1101 50-51 0.20967889507294757 1101 52-53 0.3691293776181084 1101 54-55 0.3806444060033911 1101 56-57 0.46736721528048264 1101 58-59 0.5289751987227689 1101 60-61 0.37639995967220585 1101 62-63 0.3926846061623763 1101 64-65 0.36505165094990133 1101 66-67 0.48595852797777184 1101 68-69 0.42264473541109027 1101 70-71 0.3581054039295495 1101 72-73 0.37026289132252543 1101 74-75 0.36053213059768296 1101 76-77 0.3721524129125342 1101 78-79 0.41348916087999044 1101 80-81 0.38920076848376084 1101 82-83 0.4106853025956667 1101 84-85 0.3493941820671722 1101 86-87 0.21454415829548168 1101 88-89 0.21208055321439545 1101 90-91 0.18716884282501312 1101 92-93 0.2238840793056447 1101 94-95 0.09954495478552161 1101 96-97 0.031152014148027263 1101 98-99 -0.09596048842066196 1101 100-101 0.014024679802002282 1102 1 0.2960007499791999 1102 2 0.9368642545900734 1102 3 0.5426831744059513 1102 4 0.26183130911319097 1102 5 0.21977210833141925 1102 6 0.13142917876172078 1102 7 0.1473074551794511 1102 8 0.1754850542611308 1102 9 0.09659022880922663 1102 10-11 0.20203815030125583 1102 12-13 0.2365053486064781 1102 14-15 0.17992984356737907 1102 16-17 0.20902579110174457 1102 18-19 0.18244044179328966 1102 20-21 0.21653980536204642 1102 22-23 0.18885169073228525 1102 24-25 0.15635824129791587 1102 26-27 0.25372130977883245 1102 28-29 0.21787108424659607 1102 30-31 0.2115085315688887 1102 32-33 0.1067574104937492 1102 34-35 0.10215606307378522 1102 36-37 0.22788725062758175 1102 38-39 0.1780321089352057 1102 40-41 0.17435357027292042 1102 42-43 0.22652832516757115 1102 44-45 0.08957454928122388 1102 46-47 0.11215114461332831 1102 48-49 0.04619886224917025 1102 50-51 -0.06763979445057089 1102 52-53 -0.08227552860558518 1102 54-55 0.1256162985089304 1102 56-57 0.136173321158374 1102 58-59 0.16329790402534883 1102 60-61 0.12655111235318373 1102 62-63 0.1516056965723891 1102 64-65 0.223033227583322 1102 66-67 0.16830389483988029 1102 68-69 0.12501031185514222 1102 70-71 0.08868425705303906 1102 72-73 0.12692704781905206 1102 74-75 0.11860412760322703 1102 76-77 0.1570402023896449 1102 78-79 0.2085811125052146 1102 80-81 0.20183123938477365 1102 82-83 0.21296945878643925 1102 84-85 0.1557777074605724 1102 86-87 0.10475417421952926 1102 88-89 0.17408575010493266 1102 90-91 0.05590001346677198 1102 92-93 0.12981730286218252 1102 94-95 0.03828163892474379 1102 96-97 0.002897928710513753 1102 98-99 -0.0856334068411222 1102 100-101 0.048400202240404866 1103 1 0.32611439881137727 1103 2 1.4191727327783248 1103 3 0.7327683439770283 1103 4 0.3764252236217658 1103 5 0.23699572173520522 1103 6 0.15011113515041075 1103 7 0.17015157755248822 1103 8 0.16198251965565902 1103 9 0.1492495529632052 1103 10-11 0.20163497856984947 1103 12-13 0.23340981385227622 1103 14-15 0.25708340481607905 1103 16-17 0.2698014608398509 1103 18-19 0.2256127340080667 1103 20-21 0.22204181329625072 1103 22-23 0.27971260319025504 1103 24-25 0.24881306582617668 1103 26-27 0.31368612521225714 1103 28-29 0.20581896817410694 1103 30-31 0.1588069030330601 1103 32-33 0.19272710738956533 1103 34-35 0.26788963568493074 1103 36-37 0.22584859393190726 1103 38-39 0.1752574535269531 1103 40-41 0.2735806314228739 1103 42-43 0.3798385516118259 1103 44-45 0.2823031941024112 1103 46-47 0.16067317337056153 1103 48-49 0.28165249445219587 1103 50-51 0.2356291029289963 1103 52-53 0.24394319114433927 1103 54-55 0.23399195654322824 1103 56-57 0.3443764699698306 1103 58-59 0.3383239328020764 1103 60-61 0.40061291820166645 1103 62-63 0.28606243272227516 1103 64-65 0.33856320273711304 1103 66-67 0.2685469926422428 1103 68-69 0.20357759532863184 1103 70-71 0.25381654663445374 1103 72-73 0.2862468128651585 1103 74-75 0.3715117918893114 1103 76-77 0.355788260898926 1103 78-79 0.4624426369746999 1103 80-81 0.4431020288796361 1103 82-83 0.4426312919438118 1103 84-85 0.3582550130493871 1103 86-87 0.33975404011341936 1103 88-89 0.4132852097792288 1103 90-91 0.28303431220154707 1103 92-93 0.2968985199604681 1103 94-95 0.3078219963297215 1103 96-97 0.13136097516242984 1103 98-99 0.18805473062008105 1103 100-101 0.13482342161605843 1104 1 0.3908457244787371 1104 2 1.4184546659048145 1104 3 0.7425491780936895 1104 4 0.38287180561873413 1104 5 0.23766643577354785 1104 6 0.14432419463829405 1104 7 0.14010278764123996 1104 8 0.12648721135571606 1104 9 0.11183775056352374 1104 10-11 0.14440601465459935 1104 12-13 0.17406398973625414 1104 14-15 0.1376454193611707 1104 16-17 0.13940152963103003 1104 18-19 0.1471814792521684 1104 20-21 0.12827848794928798 1104 22-23 0.11185940640270076 1104 24-25 0.15242919688751755 1104 26-27 0.1963036297414007 1104 28-29 0.20634986177628178 1104 30-31 0.05387478720943761 1104 32-33 0.07580471185439563 1104 34-35 0.11645444428288698 1104 36-37 0.19399344649026062 1104 38-39 0.21904162825655504 1104 40-41 0.24185882579565998 1104 42-43 0.2947623512410189 1104 44-45 0.2348055911924547 1104 46-47 0.125725131916802 1104 48-49 0.13936971085936278 1104 50-51 0.1801113197013251 1104 52-53 0.19225604025371013 1104 54-55 0.36822994105578744 1104 56-57 0.2899456816747019 1104 58-59 0.30022798794811223 1104 60-61 0.22007540462601582 1104 62-63 0.29024338180501985 1104 64-65 0.27163315064899507 1104 66-67 0.2734359961831032 1104 68-69 0.026655859434946194 1104 70-71 0.021379972341158293 1104 72-73 0.1690380622496832 1104 74-75 0.06283727077724421 1104 76-77 -0.009945013066676722 1104 78-79 0.05166927800958376 1104 80-81 -0.042683168294274765 1104 82-83 -0.06478166482847314 1104 84-85 -0.061909167004472465 1104 86-87 -0.09112813613807447 1104 88-89 -0.1461124433106029 1104 90-91 -0.05961881296778415 1104 92-93 -0.08056388636455125 1104 94-95 -0.24326888577585493 1104 96-97 -0.4032634885843791 1104 98-99 -0.30806704862147427 1104 100-101 -0.2640444686855652 1105 1 0.17269948329325757 1105 2 1.1933045835950047 1105 3 0.47443692553498806 1105 4 0.24144203558569188 1105 5 0.1291266909203017 1105 6 -0.007916870720876545 1105 7 7.209802470953264E-4 1105 8 -0.004073023133955189 1105 9 -0.07744864297472986 1105 10-11 -0.06772715160212073 1105 12-13 -0.1388815138004773 1105 14-15 -0.145829965502017 1105 16-17 -0.23796322793951674 1105 18-19 -0.19059369439923302 1105 20-21 -0.15340896774491597 1105 22-23 -0.23541722643913943 1105 24-25 -0.23343624364057547 1105 26-27 -0.16747966842029882 1105 28-29 -0.18382842608873062 1105 30-31 -0.16842414071556533 1105 32-33 -0.18724871808490207 1105 34-35 -0.1460014546776165 1105 36-37 -0.09263225637361217 1105 38-39 -0.06840159890668218 1105 40-41 -0.11773060797293056 1105 42-43 0.037347074832034366 1105 44-45 -0.03206189047325836 1105 46-47 -0.2086923567104293 1105 48-49 -0.3544090509354163 1105 50-51 -0.3488051756572048 1105 52-53 -0.2539264727570725 1105 54-55 -0.08094079560105882 1105 56-57 -0.1378311026827106 1105 58-59 -0.16759554328366022 1105 60-61 -0.29436236797687343 1105 62-63 -0.19379709241594867 1105 64-65 -0.12726126347717326 1105 66-67 -0.08283067394157229 1105 68-69 -0.2688702453774212 1105 70-71 -0.2763622182372174 1105 72-73 -0.4488288761439918 1105 74-75 -0.574606684388705 1105 76-77 -0.45328422047192163 1105 78-79 -0.41295843505179164 1105 80-81 -0.4325065548355056 1105 82-83 -0.40236369025942054 1105 84-85 -0.451542704087565 1105 86-87 -0.39170566979227317 1105 88-89 -0.5224912122034091 1105 90-91 -0.5898548132480634 1105 92-93 -0.7023767132263963 1105 94-95 -0.6106019934283466 1105 96-97 -0.8259059200406469 1105 98-99 -0.8384574082275158 1105 100-101 -0.9369365823231455 1106 1 0.23367283377073278 1106 2 1.2740943355657812 1106 3 0.5739159920606554 1106 4 0.2592776481281476 1106 5 0.08722619502207607 1106 6 0.09804231981939893 1106 7 0.12127672273251733 1106 8 0.08600981350002002 1106 9 -0.03673331579405925 1106 10-11 -0.029245719746064935 1106 12-13 0.007131322272719842 1106 14-15 -0.09704878159507757 1106 16-17 -0.10572027986863475 1106 18-19 -0.13336937255407122 1106 20-21 -0.2210975232310446 1106 22-23 -0.21938365116514547 1106 24-25 -0.09313670188578982 1106 26-27 -0.08884545144279343 1106 28-29 -0.23937012880615072 1106 30-31 -0.1887232218217605 1106 32-33 -0.1068963319251921 1106 34-35 -0.15089484457058688 1106 36-37 -0.1086498090878294 1106 38-39 -0.13438250001544105 1106 40-41 -0.10425763803124966 1106 42-43 0.04203367356305421 1106 44-45 -0.08866533812755506 1106 46-47 -0.0932648347595233 1106 48-49 -0.09891606681377141 1106 50-51 -0.2077035045042237 1106 52-53 -0.0019219003190755757 1106 54-55 0.06294629742591695 1106 56-57 0.06368087618353258 1106 58-59 -0.12511973734890347 1106 60-61 -0.045192286502164336 1106 62-63 0.006719735277442851 1106 64-65 -0.0809435481339662 1106 66-67 -0.07098824980839424 1106 68-69 -0.09063879549599108 1106 70-71 -0.15024940139110043 1106 72-73 -0.22408093103728532 1106 74-75 -0.2644765211188158 1106 76-77 -0.3043988398560131 1106 78-79 -0.32304208282785396 1106 80-81 -0.4411504754757729 1106 82-83 -0.49945639679022236 1106 84-85 -0.37059497969161725 1106 86-87 -0.4524579590155007 1106 88-89 -0.6666699658885982 1106 90-91 -0.6977837772293505 1106 92-93 -0.7200067863693818 1106 94-95 -0.7313965721952513 1106 96-97 -0.8321752472533639 1106 98-99 -0.791872316418246 1106 100-101 -0.814040544702177 1107 1 0.3130402529081948 1107 2 1.413189124424722 1107 3 0.6898610729231862 1107 4 0.29151737184782434 1107 5 0.1794951191011407 1107 6 0.11486060373674434 1107 7 0.0648694900281157 1107 8 0.0785974145781907 1107 9 0.03803645060306593 1107 10-11 0.12624970365914834 1107 12-13 0.1645921544829676 1107 14-15 0.10811883969872582 1107 16-17 0.0538954827099829 1107 18-19 0.11964542416649948 1107 20-21 0.09434708031792383 1107 22-23 0.08032715296782555 1107 24-25 0.022008176820413894 1107 26-27 0.0864619946488503 1107 28-29 0.022906656908126877 1107 30-31 0.0069963985735697065 1107 32-33 -0.07056083664216573 1107 34-35 0.011525135213780402 1107 36-37 0.05064657455996979 1107 38-39 -0.013678322117861796 1107 40-41 -0.049029650609867304 1107 42-43 0.1056848415774283 1107 44-45 -0.08247079073312591 1107 46-47 -0.13834288956635277 1107 48-49 -0.053483536490318784 1107 50-51 -0.12643385041616995 1107 52-53 -0.03313329407468757 1107 54-55 -0.07030140023804421 1107 56-57 0.039523896174550543 1107 58-59 0.17247123300149525 1107 60-61 0.05566477212317267 1107 62-63 0.07018138172847443 1107 64-65 0.05737209652011899 1107 66-67 0.06803283622035394 1107 68-69 0.10475793630993735 1107 70-71 -0.054951641957138975 1107 72-73 -0.03619486859344079 1107 74-75 -0.03857383693193839 1107 76-77 -0.0651419047706625 1107 78-79 -0.07934893929686382 1107 80-81 0.010321475332858654 1107 82-83 0.00559751156557553 1107 84-85 -0.22716576248586762 1107 86-87 -0.21528576112691056 1107 88-89 -0.036795050120403516 1107 90-91 -0.16772706114669234 1107 92-93 -0.35017651345832945 1107 94-95 -0.22365426536686073 1107 96-97 -0.1584401282057435 1107 98-99 -0.16176927284232434 1107 100-101 -0.07056850966174721 1108 1 0.3192556295054132 1108 2 1.4478827988095482 1108 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-1.2881094809248097 2316 76-77 -1.3312819792737116 2316 78-79 -1.456347194365918 2316 80-81 -1.4497361795211745 2316 82-83 -1.5734385428982556 2316 84-85 -1.154063611034104 2316 86-87 -1.0975603498830964 2316 88-89 -1.1754052109082345 2316 90-91 -0.9831075496930062 2316 92-93 -1.211513326348836 2316 94-95 -1.1370555196218461 2316 96-97 -0.8845517702956762 2316 98-99 -0.8734564673506036 2316 100-101 -1.1403434204401286 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10231.0 3 4093.0 4 2919.0 5 2402.0 6 2224.0 7 1949.0 8 1880.0 9 2112.0 10 2390.0 11 2937.0 12 3660.0 13 4584.0 14 5177.0 15 6305.0 16 8384.0 17 11740.0 18 10485.0 19 7130.0 20 6710.0 21 7314.0 22 7539.0 23 7913.0 24 8912.0 25 10244.0 26 12147.0 27 14672.0 28 18397.0 29 23072.0 30 29608.0 31 39505.0 32 54516.0 33 78570.0 34 122490.0 35 206872.0 36 416603.0 37 880855.0 38 926269.0 39 132993.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.56980659356854 17.91544956766187 13.379502835390902 25.135241003378685 2 17.477547704160038 19.92950097067862 37.28860229065288 25.304349034508455 3 18.993809970756377 22.8834896354502 28.948761606304828 29.17393878748859 4 13.67694431363829 15.846035356122584 35.29015749851085 35.18686283172827 5 15.314232450987586 35.720830506683285 33.71191860023535 15.253018442093778 6 34.37173919925879 34.004558902296964 17.027202871798316 14.596499026645931 7 30.167779868677158 30.605845723931285 20.55339735254448 18.672977054847074 8 27.151620841125812 33.71410276994985 19.823938690940974 19.310337697983364 9 26.787162164344814 15.713537201412816 19.44960399423451 38.04969664000786 10-11 25.8577646318854 25.14612260246027 27.19314738055065 21.802965385103683 12-13 26.466336198012407 22.27324593057703 27.312494467600658 23.94792340380991 14-15 23.61560374077547 23.79015603256177 24.728095956915986 27.866144269746773 16-17 24.11349282228628 26.85465602258147 25.388110931210978 23.643740223921274 18-19 23.994442604544464 25.94513687509592 26.776904498420826 23.283516021938787 20-21 24.373391353908925 25.435380488621302 26.665415183357382 23.52581297411239 22-23 24.41803044726231 25.123215573098 26.501763245502474 23.95699073413721 24-25 24.33863399555404 25.314803708918358 26.416828683240944 23.929733612286654 26-27 24.021834360204274 25.646941613083303 26.39874552897637 23.93247849773606 28-29 24.029246503381284 25.607813325855272 26.49582783570835 23.867112335055097 30-31 24.11513141156887 25.397966131263765 26.344148811153566 24.142753646013794 32-33 23.986286092376325 25.45248335192094 26.339567828402988 24.221662727299748 34-35 24.38261343806652 25.527211856923664 26.103283154066276 23.98689155094354 36-37 24.2418921276582 25.32488029620631 26.094473917173378 24.338753658962116 38-39 24.100344028269145 25.55461818257 26.44543479704044 23.899602992120414 40-41 24.378407749526897 25.213437621748607 26.39716519173851 24.01098943698599 42-43 24.302421708702973 25.33031450263111 26.148109214174696 24.219154574491213 44-45 24.22053060571075 25.52275726419485 26.008461169776346 24.248250960318053 46-47 24.22002797362745 25.471023195432085 25.89225905213694 24.416689778803523 48-49 24.364358902415958 25.59138992927728 25.79591932993442 24.248331838372344 50-51 24.0349563417247 25.69301131613192 25.882001186274877 24.390031155868506 52-53 24.428444652922007 25.555564170330907 25.679503866472704 24.33648731027438 54-55 24.155714477693326 25.332199887839934 25.93974433191109 24.572341302555646 56-57 24.139198748354236 25.799008833787845 25.908476149386622 24.153316268471297 58-59 24.273375080278445 25.44708794382512 25.929550091811404 24.34998688408503 60-61 24.32393534667611 25.270055153843607 26.199989823716074 24.20601967576421 62-63 24.276516812821214 25.43076216022803 26.075958364329082 24.216762662621672 64-65 24.14757560705733 25.412419385451624 25.949690868193954 24.49031413929709 66-67 24.076592228025643 25.66464456930037 25.82490362003566 24.433859582638327 68-69 23.903219089309943 25.384262843910943 26.234586798000525 24.477931268778587 70-71 23.864757570059762 25.4325933047867 26.051437573135406 24.651211552018136 72-73 24.0821659470138 25.398461096824317 25.934739662498448 24.58463329366344 74-75 23.88418964304635 25.479251177558137 25.996822576285396 24.639736603110112 76-77 24.077551367973285 25.379268155338647 26.02473780572635 24.51844267096172 78-79 23.88810198643548 25.465249455976373 26.022416245242567 24.624232312345576 80-81 23.811587638404998 25.479845984058226 26.18363086315093 24.52493551438585 82-83 23.926767850130577 25.432081734623512 25.98512374026135 24.65602667498456 84-85 23.82236470775622 25.331795256343113 25.97050859603251 24.875331439868155 86-87 23.922833498567655 25.24540810400441 26.224068079232993 24.60769031819494 88-89 23.788821230309214 25.17658404673692 26.11374556873996 24.920849154213904 90-91 23.73690867940622 25.352728600345387 26.215160488322613 24.695202231925776 92-93 23.836572081737227 25.18395044951662 26.296079714850745 24.68339775389541 94-95 23.74072336312018 25.033040076558265 26.2398412977279 24.986395262593657 96-97 23.978748865824333 24.979684656730914 26.097719589583996 24.94384688786076 98-99 23.709735328126875 24.892479627910404 26.1420841808738 25.25570086308892 100-101 23.653826354263188 24.84686032759986 26.434237341884163 25.065075976252782 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 153.0 1 119.0 2 151.5 3 212.5 4 174.5 5 184.0 6 321.0 7 636.0 8 1116.5 9 1428.0 10 1420.5 11 1414.5 12 1472.0 13 1552.5 14 1640.0 15 1667.5 16 1717.5 17 1810.0 18 1917.5 19 2043.5 20 2234.0 21 2519.5 22 3004.5 23 3584.0 24 4229.5 25 5238.5 26 6625.0 27 8505.5 28 10757.5 29 13328.0 30 16647.5 31 20799.0 32 24933.0 33 29095.0 34 34263.0 35 39128.5 36 44497.0 37 50887.5 38 56812.5 39 60541.5 40 63708.0 41 70990.0 42 79371.0 43 88199.5 44 96708.5 45 102476.5 46 108526.5 47 116714.0 48 128477.0 49 137884.5 50 142591.5 51 145318.5 52 143777.5 53 143458.0 54 143579.5 55 138548.5 56 130901.0 57 120410.5 58 108532.5 59 95547.5 60 79442.5 61 62336.5 62 48736.5 63 38029.0 64 28359.0 65 20834.5 66 14923.0 67 11091.0 68 7942.0 69 5297.5 70 3909.5 71 2827.0 72 2032.0 73 1498.0 74 1139.5 75 903.5 76 617.5 77 362.0 78 219.0 79 147.0 80 110.5 81 86.5 82 77.5 83 71.5 84 58.0 85 40.5 86 35.0 87 33.0 88 29.5 89 29.0 90 22.0 91 20.5 92 20.0 93 17.5 94 19.0 95 14.5 96 12.5 97 17.0 98 13.0 99 7.0 100 6.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055203557060095644 2 0.05129505477555991 3 0.058369120893851864 4 0.05665713228988166 5 0.05659252894633562 6 0.01075645670041653 7 0.01259765199147882 8 0.013437495457577407 9 0.00494215578127246 10-11 0.00962589818836074 12-13 0.013340590442258339 14-15 0.01510103155388807 16-17 0.02868388453444408 18-19 0.027133404289338994 20-21 0.026519672525651565 22-23 0.017071433532042452 24-25 0.006314976831625921 26-27 0.010562646669778394 28-29 0.017410601085659186 30-31 0.01558555663048341 32-33 0.01818584120821173 34-35 0.022998790302392103 36-37 0.021803628446790263 38-39 0.009189825619424934 40-41 0.009593596516587716 42-43 0.011499395151196051 44-45 0.01991398064806844 46-47 0.012904517873322535 48-49 0.013453646293463918 50-51 0.013275987098712293 52-53 0.011563998494742095 54-55 0.007720099553752404 56-57 0.011967769391904878 58-59 0.010950266731054667 60-61 0.012355389453181148 62-63 0.020430807396436802 64-65 0.014616506477292733 66-67 0.01660305929133362 68-69 0.02262732107700234 70-71 0.01708758436792896 72-73 0.022239701015726067 74-75 0.037776805138549946 76-77 0.045270792989891194 78-79 0.03393290619756025 80-81 0.01779822114693546 82-83 0.015520953286937367 84-85 0.01963941643799775 86-87 0.02311184615359768 88-89 0.01994628231984146 90-91 0.023580220394306507 92-93 0.04636904983017396 94-95 0.07182276718731578 96-97 0.08752137966900476 98-99 0.09033162511325774 100-101 0.07479452099043385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3095815.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.432615805132954 #Duplication Level Percentage of deduplicated Percentage of total 1 80.72167183493848 45.55335093809105 2 10.345522087744424 11.676497465623962 3 3.331005433578905 5.63932049633906 4 1.6404173427631736 3.7029216665692513 5 0.9320864145388932 2.6300037264428626 6 0.618699933356401 2.0948913382657888 7 0.4290745687024801 1.6949660201138128 8 0.3150006038385781 1.4221046443845893 9 0.2358275359468052 1.1977528259121515 >10 1.2801673058419296 13.118402783909753 >50 0.0919908498896629 3.5516475376274026 >100 0.054425233772792195 5.602850211995607 >500 0.003184465208858288 1.2340364502143735 >1k 9.263898785381238E-4 0.881253894510198 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4315 0.13938171370059257 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3150 0.10175026608502122 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.2301671773022613E-5 0.0 5 0.0 0.0 0.0 3.2301671773022613E-5 0.0 6 0.0 0.0 0.0 3.2301671773022613E-5 0.0 7 0.0 0.0 0.0 3.2301671773022613E-5 0.0 8 0.0 0.0 0.0 3.2301671773022613E-5 0.0 9 0.0 0.0 0.0 3.2301671773022613E-5 0.0 10-11 0.0 0.0 0.0 3.2301671773022613E-5 0.0 12-13 3.2301671773022613E-5 0.0 0.0 6.460334354604523E-5 6.460334354604523E-5 14-15 3.2301671773022613E-5 0.0 0.0 9.690501531906784E-5 6.460334354604523E-5 16-17 4.845250765953392E-5 0.0 0.0 9.690501531906784E-5 6.460334354604523E-5 18-19 9.690501531906784E-5 0.0 0.0 1.2920668709209045E-4 9.690501531906784E-5 20-21 1.2920668709209045E-4 0.0 0.0 1.2920668709209045E-4 9.690501531906784E-5 22-23 1.2920668709209045E-4 0.0 0.0 1.6150835886511307E-4 9.690501531906784E-5 24-25 1.6150835886511307E-4 0.0 0.0 1.9381003063813568E-4 9.690501531906784E-5 26-27 1.6150835886511307E-4 0.0 0.0 3.5531838950324875E-4 9.690501531906784E-5 28-29 1.7765919475162437E-4 0.0 0.0 0.0015020277374455514 1.2920668709209045E-4 30-31 1.9381003063813568E-4 0.0 0.0 0.006121166800987786 1.2920668709209045E-4 32-33 1.9381003063813568E-4 0.0 0.0 0.016247740901830374 1.2920668709209045E-4 34-35 2.261117024111583E-4 0.0 0.0 0.02978214137472685 1.2920668709209045E-4 36-37 2.584133741841809E-4 0.0 0.0 0.052005691554566406 1.4535752297860176E-4 38-39 2.907150459572035E-4 0.0 0.0 0.08742447465368569 1.9381003063813568E-4 40-41 3.0686588184371483E-4 0.0 0.0 0.12625108412485886 2.09960866524647E-4 42-43 3.3916755361673744E-4 0.0 0.0 0.171457273771204 2.584133741841809E-4 44-45 4.522234048223166E-4 0.0 0.0 0.2298425455009424 2.907150459572035E-4 46-47 5.81430091914407E-4 0.0 0.0 0.2971269278041485 3.2301671773022613E-4 48-49 6.137317636874297E-4 0.0 0.0 0.3687074324531666 3.5531838950324875E-4 50-51 6.137317636874297E-4 0.0 0.0 0.4452623945552302 3.5531838950324875E-4 52-53 6.137317636874297E-4 0.0 0.0 0.5294728528675001 3.5531838950324875E-4 54-55 6.460334354604523E-4 1.6150835886511307E-5 0.0 0.6295918845279838 3.7146922538976006E-4 56-57 6.460334354604523E-4 3.2301671773022613E-5 0.0 0.7505616453179534 4.0377089716278267E-4 58-59 6.460334354604523E-4 3.2301671773022613E-5 0.0 0.8804143658455044 4.19921733049294E-4 60-61 6.460334354604523E-4 3.2301671773022613E-5 0.0 1.019263101961842 4.19921733049294E-4 62-63 6.460334354604523E-4 3.2301671773022613E-5 0.0 1.1680284513124977 4.19921733049294E-4 64-65 6.460334354604523E-4 3.2301671773022613E-5 0.0 1.3407454902828495 4.19921733049294E-4 66-67 6.783351072334749E-4 3.2301671773022613E-5 0.0 1.5441006649299134 4.360725689358053E-4 68-69 7.106367790064975E-4 3.2301671773022613E-5 0.0 1.7702608198487313 4.522234048223166E-4 70-71 7.267876148930088E-4 3.2301671773022613E-5 0.0 2.01780468148129 4.845250765953392E-4 72-73 7.590892866660314E-4 3.2301671773022613E-5 0.0 2.273440111892991 5.81430091914407E-4 74-75 7.752401225525427E-4 3.2301671773022613E-5 0.0 2.5623785659026783 5.81430091914407E-4 76-77 7.752401225525427E-4 3.2301671773022613E-5 0.0 2.8936806624426845 5.81430091914407E-4 78-79 7.752401225525427E-4 3.2301671773022613E-5 0.0 3.252681442528058 5.81430091914407E-4 80-81 8.39843466098588E-4 3.2301671773022613E-5 0.0 3.6340834319880226 5.975809278009183E-4 82-83 8.39843466098588E-4 3.2301671773022613E-5 0.0 4.032686061667121 6.137317636874297E-4 84-85 8.721451378716106E-4 3.2301671773022613E-5 0.0 4.462459804607187 6.137317636874297E-4 86-87 9.044468096446332E-4 3.2301671773022613E-5 0.0 4.9389740666028175 6.460334354604523E-4 88-89 9.044468096446332E-4 3.2301671773022613E-5 0.0 5.457092881842099 6.460334354604523E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2855 0.0 45.770863 1 GTATCAA 5225 0.0 40.743114 1 TCAACGC 5835 0.0 35.9934 4 ATCAACG 5840 0.0 35.963745 3 CAACGCA 6055 0.0 34.683388 5 AACGCAG 6315 0.0 33.39293 6 TATCAAC 6250 0.0 32.617207 2 ACGCAGA 7315 0.0 28.958261 7 CGCAGAG 7760 0.0 27.113586 8 GTACATG 7965 0.0 24.57955 1 TACATGG 8420 0.0 22.969515 2 ACATGGG 8420 0.0 22.743036 3 GCAGAGT 9330 0.0 22.295815 9 CATGGGG 7085 0.0 20.119762 4 GTGGTAT 1590 0.0 19.126934 1 CAGAGTA 8765 0.0 18.776161 10-11 GAGTACT 4925 0.0 18.564959 12-13 AGAGTAC 8450 0.0 18.183313 10-11 GTACTTT 5215 0.0 17.898487 14-15 CCTATAA 1695 0.0 17.101044 1 >>END_MODULE