FastQCFastQC Report
Fri 27 May 2016
SRR940223_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940223_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3095815
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50120.16189597892638932No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37540.1212604758359269No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31700.047.961931
GTATCAA57600.039.841031
ATCAACG66050.033.371363
TCAACGC66900.032.947364
TATCAAC70100.031.9859872
CAACGCA69650.031.577275
AACGCAG72450.030.5511256
GTACATG81800.026.4860651
ACGCAGA84350.026.0157627
ACATGGG83900.024.6861463
CGCAGAG88850.024.6442818
TACATGG86400.024.5770382
GTGGTAT15600.021.3196161
GCAGAGT102650.021.146089
CATGGGG70050.021.02244
TATAAGC16700.019.3428823
AGAGTAC97050.018.5488210-11
CTATAAG17400.018.2920062
CCTATAA17000.018.1664621
CAGAGTA100850.017.75571310-11
Produced by FastQC (version 0.11.5)