##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940223_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3095815 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.74032298441606 34.0 31.0 34.0 31.0 34.0 2 32.8130208038917 34.0 31.0 34.0 31.0 34.0 3 32.87421599804898 34.0 31.0 34.0 31.0 34.0 4 36.29868645251735 37.0 37.0 37.0 35.0 37.0 5 36.25859071036221 37.0 37.0 37.0 35.0 37.0 6 36.292714519439954 37.0 37.0 37.0 35.0 37.0 7 36.25949321907155 37.0 37.0 37.0 35.0 37.0 8 36.25292241299948 37.0 37.0 37.0 35.0 37.0 9 38.047931158677116 39.0 38.0 39.0 35.0 39.0 10-11 37.98090938896543 39.0 38.0 39.0 35.0 39.0 12-13 37.92568192866822 39.0 38.0 39.0 35.0 39.0 14-15 39.326240909098246 41.0 39.0 41.0 36.0 41.0 16-17 39.28328485390762 40.5 39.0 41.0 36.0 41.0 18-19 39.22889788310994 40.0 39.0 41.0 36.0 41.0 20-21 39.14841923693761 40.0 39.0 41.0 36.0 41.0 22-23 39.05658235391972 40.0 39.0 41.0 36.0 41.0 24-25 38.95003512806805 40.0 38.0 41.0 35.0 41.0 26-27 38.72721141282667 40.0 38.0 41.0 35.0 41.0 28-29 38.87661714282022 40.0 38.0 41.0 35.0 41.0 30-31 38.74398938567066 40.0 38.0 41.0 35.0 41.0 32-33 38.58528901113277 40.0 38.0 41.0 35.0 41.0 34-35 38.73321726265943 40.0 38.0 41.0 35.0 41.0 36-37 38.69125771404299 40.0 38.0 41.0 35.0 41.0 38-39 38.57226643064912 40.0 38.0 41.0 35.0 41.0 40-41 38.407551646335456 40.0 38.0 41.0 34.0 41.0 42-43 38.25098479721818 40.0 38.0 41.0 34.0 41.0 44-45 38.070392610669565 40.0 38.0 41.0 33.0 41.0 46-47 37.83558852838429 40.0 37.5 41.0 33.0 41.0 48-49 37.61670933179147 40.0 37.0 41.0 33.0 41.0 50-51 37.3788556486741 40.0 37.0 41.0 32.5 41.0 52-53 37.146146975836736 39.5 36.5 41.0 32.0 41.0 54-55 36.90613602557001 39.0 36.0 41.0 31.0 41.0 56-57 36.651751154380996 39.0 35.5 40.5 31.0 41.0 58-59 36.31277805682833 38.5 35.0 40.0 31.0 41.0 60-61 35.94249898653505 38.0 35.0 40.0 30.0 41.0 62-63 35.583270964188756 37.5 35.0 40.0 29.5 41.0 64-65 35.18789882470367 37.0 34.0 39.5 29.0 41.0 66-67 34.743735171513805 36.0 34.0 39.0 29.0 40.5 68-69 34.27544362308471 36.0 34.0 39.0 28.0 40.0 70-71 33.84828599254155 35.0 33.5 38.0 28.0 40.0 72-73 33.38244581798331 35.0 33.0 37.0 26.5 39.0 74-75 32.92778186035018 35.0 33.0 37.0 26.0 39.0 76-77 31.60554716609358 34.0 30.5 35.5 25.5 37.0 78-79 32.35882441295749 35.0 32.5 36.0 26.0 37.0 80-81 32.22490862664597 35.0 32.5 35.0 26.0 37.0 82-83 31.948422305596427 35.0 32.0 35.0 26.0 36.5 84-85 31.63899199403065 34.5 32.0 35.0 25.0 36.0 86-87 31.30661328277045 34.0 32.0 35.0 24.5 36.0 88-89 30.97452464052277 34.0 31.0 35.0 23.5 35.0 90-91 30.653192454975507 34.0 31.0 35.0 21.5 35.0 92-93 30.30503373102075 34.0 31.0 35.0 19.5 35.0 94-95 29.922299782125222 34.0 30.5 35.0 17.5 35.0 96-97 29.39579303026828 34.0 30.0 35.0 6.0 35.0 98-99 28.7276455472953 34.0 29.5 35.0 2.0 35.0 100-101 25.929607066313714 31.5 24.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1216045883244945 1101 2 0.035989607905243304 1101 3 0.10787933085621404 1101 4 0.088513551533552 1101 5 0.05061609619126273 1101 6 0.09634430130977023 1101 7 2.027601745027141E-4 1101 8 -0.002509625238339197 1101 9 0.030708491462519305 1101 10-11 0.08335834995191505 1101 12-13 0.14716657409229583 1101 14-15 0.13867359471384333 1101 16-17 0.07944548258907247 1101 18-19 0.13607157113283108 1101 20-21 0.1072866813348412 1101 22-23 0.13518348047158923 1101 24-25 0.07226050999855005 1101 26-27 0.13573646807980566 1101 28-29 0.08693793282127871 1101 30-31 -0.004593920184269962 1101 32-33 0.1509716358206532 1101 34-35 0.12376405703925997 1101 36-37 0.06697092676032668 1101 38-39 0.09833585495371722 1101 40-41 0.15994539795943297 1101 42-43 0.2184238647313279 1101 44-45 0.2618857176797391 1101 46-47 0.1795551255094736 1101 48-49 0.1753043854320282 1101 50-51 0.08877999480851173 1101 52-53 0.1132701521233912 1101 54-55 0.19931781582453567 1101 56-57 0.15738155422712907 1101 58-59 0.0956393793168715 1101 60-61 0.2001195910320206 1101 62-63 0.19939955123593478 1101 64-65 0.2011875469384421 1101 66-67 0.2949368249483726 1101 68-69 0.28147484014665025 1101 70-71 0.21565499561567236 1101 72-73 0.14226405749608517 1101 74-75 0.1433167835512279 1101 76-77 -0.009792173674643578 1101 78-79 0.18671458514555184 1101 80-81 0.1967742568964681 1101 82-83 0.21823586758947755 1101 84-85 0.25473098147153195 1101 86-87 0.38673954496607266 1101 88-89 0.2875465694840109 1101 90-91 0.23123228162033982 1101 92-93 0.44518609283321453 1101 94-95 0.45316691848503865 1101 96-97 0.3313921459469107 1101 98-99 0.16113040731557504 1101 100-101 0.2995389907174335 1102 1 0.005582946614048012 1102 2 0.025380070540947486 1102 3 0.05961600858439908 1102 4 0.007735142350178137 1102 5 -0.010757563592406427 1102 6 0.06344972993488085 1102 7 0.016501112388361605 1102 8 0.04323169112644365 1102 9 0.06405158930835597 1102 10-11 0.06131705888609673 1102 12-13 0.07009066585702328 1102 14-15 0.12757928382458772 1102 16-17 0.1561962769420262 1102 18-19 0.05831042097189254 1102 20-21 0.15977697723409534 1102 22-23 0.09032072365381083 1102 24-25 0.21174644786764674 1102 26-27 0.17412937233678605 1102 28-29 0.047525549348158336 1102 30-31 0.21974661768255288 1102 32-33 0.1879692750771511 1102 34-35 0.16342967673319464 1102 36-37 0.201794926353962 1102 38-39 0.1440343899316332 1102 40-41 0.18148933999175654 1102 42-43 0.045940408922412246 1102 44-45 0.025098631888631928 1102 46-47 -0.06430716330212505 1102 48-49 0.017385587709817685 1102 50-51 0.06953453518352859 1102 52-53 0.06116390239154157 1102 54-55 0.026721550306731956 1102 56-57 0.08368759476169885 1102 58-59 0.12334544381887724 1102 60-61 0.1924272017790969 1102 62-63 0.11057885776813947 1102 64-65 0.007321411500704755 1102 66-67 0.05330311351141859 1102 68-69 0.09260993513612448 1102 70-71 0.08923032611730264 1102 72-73 0.13877349085353075 1102 74-75 0.2107167009967199 1102 76-77 0.07226992369332663 1102 78-79 0.25404804965870653 1102 80-81 0.22351188395224852 1102 82-83 0.09177248525111281 1102 84-85 0.08663391235407403 1102 86-87 0.010431260566260647 1102 88-89 0.10321451885254618 1102 90-91 0.0073220234081645685 1102 92-93 -0.0160139016434222 1102 94-95 -0.11305151760499399 1102 96-97 -0.2855664944384202 1102 98-99 -0.3253810036022351 1102 100-101 -0.09555167869907777 1103 1 0.10273916560201002 1103 2 0.023545255131907084 1103 3 0.06505754685462506 1103 4 0.007822433236086113 1103 5 0.05540722111852148 1103 6 0.057034093650159434 1103 7 -0.041145983726707414 1103 8 -0.05388039475998596 1103 9 0.015472016838636193 1103 10-11 0.027473187657221843 1103 12-13 0.043559356957004525 1103 14-15 -0.02562329198803326 1103 16-17 0.03232563325708071 1103 18-19 0.01834119222831987 1103 20-21 -0.020334355219830513 1103 22-23 0.020114346542563055 1103 24-25 -0.02681917756667218 1103 26-27 0.043946597900827555 1103 28-29 -0.004847763048367426 1103 30-31 -0.07115684298656078 1103 32-33 -0.08366850188244257 1103 34-35 -0.17509205942888428 1103 36-37 -0.07957151737145551 1103 38-39 -0.049479385204115545 1103 40-41 0.02646670044215682 1103 42-43 -0.0777198100850569 1103 44-45 -0.08012821469154119 1103 46-47 -0.12324399444789691 1103 48-49 -0.11690156516871042 1103 50-51 -0.05582127270825055 1103 52-53 -0.14090108786747635 1103 54-55 -0.1907338228374229 1103 56-57 -0.19787549728629727 1103 58-59 -0.21624414271979475 1103 60-61 -0.07272910498740259 1103 62-63 -0.22783364287190722 1103 64-65 -0.20202188307189317 1103 66-67 -0.1930571247585604 1103 68-69 -0.21028175833144047 1103 70-71 -0.4918065823223614 1103 72-73 -0.3478271143307623 1103 74-75 -0.18229076261785337 1103 76-77 -0.2913066131017352 1103 78-79 -0.11432985160506348 1103 80-81 0.030037609161517764 1103 82-83 -0.18848599817946976 1103 84-85 -0.2001381048212636 1103 86-87 -0.13335889506997844 1103 88-89 -0.22528046923639167 1103 90-91 -0.16650180505305556 1103 92-93 -0.15916071369736784 1103 94-95 -0.21467407961687712 1103 96-97 -0.15708467313115548 1103 98-99 -0.3616986448952133 1103 100-101 -0.059614533013515825 1104 1 -0.0394972075006379 1104 2 -0.016725857518366638 1104 3 0.006250037307488299 1104 4 0.00799017064223051 1104 5 -0.03269543794342411 1104 6 -0.0030729096221335794 1104 7 0.016931679634680563 1104 8 -0.034838194195991434 1104 9 -0.011966477482559412 1104 10-11 0.02285663960822859 1104 12-13 -0.009932031187823043 1104 14-15 0.05610251860533566 1104 16-17 0.011704651474964578 1104 18-19 0.06272870333032188 1104 20-21 0.051878836196351585 1104 22-23 0.006107566370438633 1104 24-25 -0.01364048304429133 1104 26-27 0.08092291912738148 1104 28-29 0.011602046480156503 1104 30-31 0.03783053665831204 1104 32-33 0.10581799078022414 1104 34-35 0.05401627019397637 1104 36-37 0.01709328816388478 1104 38-39 0.09872073588721264 1104 40-41 0.1556977704984419 1104 42-43 0.19940888832449843 1104 44-45 0.15947701343839782 1104 46-47 -0.014748636674845272 1104 48-49 0.1854492321729353 1104 50-51 0.2419168637574458 1104 52-53 0.18797672061504045 1104 54-55 0.1348550838774898 1104 56-57 0.18283658569660588 1104 58-59 0.2623410676469433 1104 60-61 -0.015535950181330804 1104 62-63 -0.13836152097016452 1104 64-65 -0.0957754315897077 1104 66-67 -0.05260708435552175 1104 68-69 0.04511399122153392 1104 70-71 0.00996526182320423 1104 72-73 0.06726194904900495 1104 74-75 0.0247480831980198 1104 76-77 -0.09576213795254773 1104 78-79 0.0893968871981059 1104 80-81 0.05208082510223022 1104 82-83 0.015702371869977583 1104 84-85 0.015708486127984145 1104 86-87 0.07134588002761788 1104 88-89 0.0708711116651024 1104 90-91 0.07281000040924823 1104 92-93 0.05060951903828226 1104 94-95 -0.06999526612344198 1104 96-97 -0.1204695056175531 1104 98-99 0.05981273509833329 1104 100-101 0.1509538659188081 1105 1 -0.07989339567842535 1105 2 -0.10972297189760383 1105 3 -0.1157327326882438 1105 4 -0.14994319344921792 1105 5 -0.08630169039474112 1105 6 -0.13235185933261562 1105 7 -0.08906688073994928 1105 8 -0.060490052377424774 1105 9 -0.11277278599008866 1105 10-11 -0.030541335816216986 1105 12-13 -0.0758972672381546 1105 14-15 -0.149698047780781 1105 16-17 -0.11326304340453675 1105 18-19 -0.1503911341066697 1105 20-21 -0.19511383008629224 1105 22-23 -0.1334097177790312 1105 24-25 -0.10731202368913273 1105 26-27 -0.015535279411700742 1105 28-29 -0.19303917558161032 1105 30-31 -0.11116955161201503 1105 32-33 -0.10281268616219563 1105 34-35 -0.023328444551815153 1105 36-37 0.005056673267311851 1105 38-39 -0.11989665955567119 1105 40-41 -0.1253552990795086 1105 42-43 -0.38730180885639953 1105 44-45 -0.21943702491096673 1105 46-47 -0.10255167918020192 1105 48-49 -0.28076018053817364 1105 50-51 -0.48306509542526754 1105 52-53 -0.15579503415360563 1105 54-55 -0.1890155791600776 1105 56-57 -0.17533585903662186 1105 58-59 -0.39294327656366335 1105 60-61 -0.5642977995630218 1105 62-63 -0.3236630096447115 1105 64-65 -0.4952845530087231 1105 66-67 -0.5849916026497723 1105 68-69 -0.8069836121025276 1105 70-71 -0.7253629809511395 1105 72-73 -0.7152525859802097 1105 74-75 -0.5656455114700947 1105 76-77 -0.8831184597916284 1105 78-79 -0.7293212228704391 1105 80-81 -0.582788957962066 1105 82-83 -0.679424537203392 1105 84-85 -0.5790252559788698 1105 86-87 -0.708508373828753 1105 88-89 -0.7802531292783179 1105 90-91 -0.7014790139387763 1105 92-93 -0.8107305337601147 1105 94-95 -0.9419509635181917 1105 96-97 -0.9264457481589403 1105 98-99 -0.9808993380076991 1105 100-101 -0.6957951924551793 1106 1 -0.07224813114596174 1106 2 -0.002360305262982365 1106 3 0.014324387737318034 1106 4 0.054653879229114466 1106 5 0.020498684533151845 1106 6 0.060293385171647174 1106 7 -0.03601568465585814 1106 8 0.03563579281260587 1106 9 -0.1345479454145604 1106 10-11 -0.023336190972280235 1106 12-13 0.019700834889647467 1106 14-15 0.015647759115331894 1106 16-17 0.005355721816897585 1106 18-19 -0.07558756067527383 1106 20-21 0.08873032248052226 1106 22-23 -0.143970837836072 1106 24-25 -0.05427634945981907 1106 26-27 0.01754379592637889 1106 28-29 -0.06454851072548706 1106 30-31 0.057910012414133405 1106 32-33 0.10166196414543549 1106 34-35 0.023975158921878403 1106 36-37 -0.15529560273730425 1106 38-39 -0.12387325860713361 1106 40-41 -0.03873183323960916 1106 42-43 0.13869279154095437 1106 44-45 -0.06315848472637953 1106 46-47 0.13200607814211196 1106 48-49 0.17809063140504122 1106 50-51 0.13957107630452725 1106 52-53 0.050732005918817435 1106 54-55 0.06496177094887656 1106 56-57 0.09344182994946948 1106 58-59 -0.02057322953745455 1106 60-61 -0.0745692617818321 1106 62-63 -0.07287665245379316 1106 64-65 0.14213643574343138 1106 66-67 -0.03582262272603742 1106 68-69 0.023967116523373022 1106 70-71 -0.0255418828450118 1106 72-73 0.03375226284693866 1106 74-75 0.08640933174916654 1106 76-77 -0.23058869788686565 1106 78-79 -0.019309526401812604 1106 80-81 0.208168960787539 1106 82-83 0.21414085286582463 1106 84-85 0.11007349506259345 1106 86-87 0.15596129947125448 1106 88-89 0.10624355805745367 1106 90-91 0.22984328786215968 1106 92-93 0.1686131321214468 1106 94-95 0.0957979369336428 1106 96-97 -0.014400838926743376 1106 98-99 -0.3644008080775585 1106 100-101 -0.4570575498543974 1107 1 0.053432742618639395 1107 2 0.05982382546478249 1107 3 0.10672667884244191 1107 4 0.055341772310875115 1107 5 0.10414086782156318 1107 6 0.11709369963705285 1107 7 0.09165867518241555 1107 8 0.15688045813156037 1107 9 0.10345604155758537 1107 10-11 0.19438898872224541 1107 12-13 0.18341236903160052 1107 14-15 0.18611889568316542 1107 16-17 0.26002707738742714 1107 18-19 0.2908664206814109 1107 20-21 0.26495027980306673 1107 22-23 0.24072634882161026 1107 24-25 0.2456029182850159 1107 26-27 0.2832460084151478 1107 28-29 0.38753412067612913 1107 30-31 0.2728906390987689 1107 32-33 0.35491541764947954 1107 34-35 0.267138344007229 1107 36-37 0.2677937391315126 1107 38-39 0.28730430203976454 1107 40-41 0.25186087790145706 1107 42-43 0.29738551391292134 1107 44-45 0.2628256235395625 1107 46-47 0.3104862555904546 1107 48-49 0.24176636702948429 1107 50-51 0.2784623049658066 1107 52-53 0.40219272581998666 1107 54-55 0.4042580142103276 1107 56-57 0.42065782837714494 1107 58-59 0.3661174431939074 1107 60-61 0.3334636447770194 1107 62-63 0.3080230836198794 1107 64-65 0.1520406922573585 1107 66-67 0.22687883167647271 1107 68-69 0.2335604172870802 1107 70-71 0.19159016454941735 1107 72-73 0.2235721471685892 1107 74-75 0.05900240789470956 1107 76-77 0.05968814398195832 1107 78-79 0.3726210665900993 1107 80-81 0.4226126097093683 1107 82-83 0.5585682169718496 1107 84-85 0.5266629492404888 1107 86-87 0.5767904212142945 1107 88-89 0.50042735185799 1107 90-91 0.5134658170625173 1107 92-93 0.685540215454779 1107 94-95 0.6734140364394854 1107 96-97 0.5980731895997629 1107 98-99 0.5865271456511003 1107 100-101 0.6851467965067854 1108 1 -0.0868762181815228 1108 2 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#Quality Count 2 2.0 3 6.0 4 17.0 5 40.0 6 79.0 7 197.0 8 463.0 9 885.0 10 1425.0 11 2274.0 12 3507.0 13 4788.0 14 6083.0 15 7664.0 16 10408.0 17 14781.0 18 14465.0 19 10618.0 20 10146.0 21 11257.0 22 11590.0 23 12968.0 24 15170.0 25 18545.0 26 22793.0 27 28416.0 28 35411.0 29 43571.0 30 54007.0 31 67993.0 32 86899.0 33 116017.0 34 166989.0 35 264283.0 36 493094.0 37 879448.0 38 638501.0 39 41012.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.27984789523251 18.478144631990787 13.748907595061441 24.493099877715263 2 16.589372601754725 20.145341052726106 37.64886927348532 25.616417072033848 3 19.326966435859635 23.34589011449045 29.11802555975766 28.209117889892255 4 14.179688572540696 16.428323392269068 35.150827991323574 34.241160043866664 5 15.382637378895916 36.03921202514165 33.5227538869117 15.05539670905074 6 34.35299536759032 34.39036861913103 16.809047880077408 14.44758813320124 7 30.126655468699525 30.921183538988306 20.44712190709994 18.505039085212225 8 27.21092830159425 33.96175159045357 19.661995306567093 19.165324801385093 9 26.836939545806192 15.754655882215186 19.348604487025227 38.0598000849534 10-11 25.986872600591443 25.332634540500642 27.05702698643168 21.62346587247623 12-13 26.53412752376999 22.475535521340905 27.214804502207013 23.77553245268209 14-15 23.659763261047576 23.981940135311703 24.660372147560498 27.697924456080226 16-17 24.072077950394323 27.117528017662558 25.312720559852576 23.497673472090547 18-19 24.071561123645953 26.044789498080473 26.829122541237123 23.054526837036452 20-21 24.473374107565675 25.487911475941498 26.70427418786942 23.334440228623407 22-23 24.483007622065212 25.19681008019053 26.56988976568202 23.750292532062243 24-25 24.367950942630735 25.422588122503882 26.476094739847873 23.733366195017513 26-27 24.05034546693757 25.745410732570797 26.493841676330266 23.710402124161366 28-29 24.05190188135356 25.773846074830992 26.52615492652472 23.64809711729073 30-31 24.242040047677342 25.483379147945122 26.383725099408554 23.890855704968974 32-33 24.081769442409865 25.522118433899077 26.380788024979186 24.015324098711872 34-35 24.489038144701368 25.547939331420743 26.134959793152245 23.82806273072564 36-37 24.300750097026633 25.405020577075486 26.23344954994663 24.060779775951254 38-39 24.21943817702285 25.59758900321886 26.504070818185195 23.67890200157309 40-41 24.456369534664145 25.301125037833433 26.446367619170058 23.796137808332368 42-43 24.377522558680024 25.40904414507973 26.148251752769465 24.065181543470782 44-45 24.303876423721203 25.608141599380712 25.96788653805497 24.120095438843116 46-47 24.3119309026867 25.59781464047457 25.858774064086482 24.23148039275225 48-49 24.475204617385355 25.73312750462081 25.79212721325625 23.999540664737587 50-51 24.171001820199205 25.735824007571512 25.884653960265712 24.20852021196357 52-53 24.612048595958477 25.622526296000437 25.5935839793941 24.17184112864698 54-55 24.314061049947462 25.44735710688667 25.806441192935058 24.432140650230814 56-57 24.26348150648537 25.901919203828395 25.847070965157805 23.987528324528437 58-59 24.408059082408602 25.509547335165593 25.882050635188868 24.200342947236937 60-61 24.435305938164557 25.361201506486097 26.139995190278743 24.063497365070603 62-63 24.41433350006404 25.533232735013982 25.981871306399405 24.070562458522573 64-65 24.253908704852023 25.48202770216786 25.86353454517733 24.400529047802785 66-67 24.111915873322225 25.75484748324952 25.78127031477248 24.35196632865577 68-69 23.92722041027672 25.50334920100148 26.13400744812429 24.43542294059751 70-71 23.968034265613415 25.5017499430683 25.960159118035154 24.570056673283126 72-73 24.108160664649933 25.540133052734948 25.82846243174143 24.523243850873694 74-75 23.97096530993141 25.543055881264188 25.813081793673653 24.672897015130754 76-77 24.086992362585324 25.46125216905936 25.89155930657288 24.560196161782432 78-79 23.803005024525046 25.55784179610216 25.903453533237613 24.73569964613519 80-81 23.758138002432318 25.61561333606821 25.996821515497537 24.62942714600194 82-83 23.830118499065673 25.53744502641478 25.84844652683228 24.783989947687274 84-85 23.790132676786634 25.424834468960146 25.766087795247355 25.018945059005866 86-87 23.853287022867477 25.3282985103072 26.00670753422611 24.811706932599215 88-89 23.612473613571872 25.3409522209822 25.882796614138766 25.16377755130717 90-91 23.50490774429177 25.465017513410636 26.06236476508837 24.96770997720923 92-93 23.547032090912218 25.264242424829753 26.119706126858006 25.069019357400023 94-95 23.458879164291147 25.149952435788315 26.03222414776077 25.35894425215977 96-97 23.598284124110286 25.074609268378556 25.88637167512707 25.44073493238409 98-99 23.226858861768925 24.99999596228777 25.97965800577393 25.79348717016938 100-101 23.078058678837007 24.982333873398602 26.258139137847795 25.681468309916593 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 103.0 1 93.5 2 138.5 3 187.0 4 147.5 5 148.0 6 294.5 7 665.0 8 1101.0 9 1287.5 10 1269.0 11 1262.5 12 1387.5 13 1504.5 14 1613.5 15 1762.0 16 1843.0 17 1869.5 18 1971.5 19 2155.0 20 2365.0 21 2641.0 22 3020.0 23 3554.5 24 4203.5 25 5099.5 26 6503.0 27 8433.0 28 10664.0 29 13378.5 30 16841.0 31 20842.0 32 24916.5 33 29206.0 34 34385.0 35 39434.5 36 44735.0 37 50994.0 38 56988.0 39 60525.0 40 63985.0 41 71500.5 42 79949.5 43 88567.5 44 96704.0 45 102992.5 46 109088.5 47 117367.0 48 129498.0 49 139102.5 50 143645.0 51 145775.5 52 144009.5 53 143761.5 54 143831.0 55 138186.0 56 130680.5 57 120378.0 58 108168.5 59 95156.5 60 78821.0 61 61478.0 62 47762.5 63 37115.5 64 28035.5 65 20708.0 66 14588.0 67 10858.0 68 7701.0 69 5006.0 70 3554.0 71 2548.0 72 1778.0 73 1238.5 74 977.0 75 750.0 76 496.5 77 263.5 78 130.5 79 73.0 80 43.5 81 24.0 82 12.5 83 10.0 84 6.5 85 3.0 86 1.0 87 1.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.018734969628353113 2 0.002002703649927402 3 3.5531838950324875E-4 4 0.002261117024111583 5 0.007558591194887292 6 5.81430091914407E-4 7 4.8452507659533915E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.00222881535233856 22-23 1.6150835886511307E-5 24-25 3.5531838950324875E-4 26-27 1.6150835886511307E-4 28-29 3.8762006127627136E-4 30-31 1.6150835886511307E-4 32-33 0.001211312691488348 34-35 3.7146922538976006E-4 36-37 5.006759124818505E-4 38-39 0.0 40-41 2.584133741841809E-4 42-43 0.0 44-45 3.3916755361673744E-4 46-47 0.003973105628081781 48-49 0.0011951618556018367 50-51 0.0 52-53 6.460334354604523E-5 54-55 7.106367790064975E-4 56-57 0.0 58-59 1.1305585120557913E-4 60-61 4.845250765953392E-5 62-63 1.4535752297860176E-4 64-65 0.0020834578293599587 66-67 2.4226253829766957E-4 68-69 6.460334354604523E-5 70-71 0.0 72-73 0.0016150835886511307 74-75 3.2301671773022613E-5 76-77 9.690501531906784E-5 78-79 0.0 80-81 0.0 82-83 3.2301671773022613E-4 84-85 6.783351072334749E-4 86-87 0.001372821050353461 88-89 0.0 90-91 0.0014374243938995064 92-93 0.004053859807514338 94-95 0.0 96-97 6.460334354604523E-4 98-99 8.075417943255653E-5 100-101 0.006508786862264057 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3095815.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.29245272177944 #Duplication Level Percentage of deduplicated Percentage of total 1 80.2347033893562 44.36373543801974 2 10.564113306048013 11.682314710443615 3 3.453391854103439 5.7283951746837785 4 1.6425163306479904 3.632750262284186 5 0.9994952912675165 2.7632273069025164 6 0.6216409724108067 2.0623232446167314 7 0.42404022577363154 1.641235689500484 8 0.3219657491172448 1.4241820768878046 9 0.24592517364949712 1.2238025433399198 >10 1.3294938936240555 13.376590298308107 >50 0.09997087806256971 3.7935040777352635 >100 0.058474753618093435 6.028971781878703 >500 0.003215753803640514 1.2571272185683002 >1k 9.939602663009287E-4 0.8598095843770543 >5k 5.8468250958878166E-5 0.16203059245366958 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5012 0.16189597892638932 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3754 0.1212604758359269 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.2301671773022613E-5 2 0.0 0.0 0.0 0.0 3.2301671773022613E-5 3 0.0 0.0 0.0 0.0 3.2301671773022613E-5 4 0.0 0.0 0.0 0.0 3.2301671773022613E-5 5 3.2301671773022613E-5 6.460334354604523E-5 0.0 0.0 3.2301671773022613E-5 6 6.460334354604523E-5 6.460334354604523E-5 0.0 0.0 3.2301671773022613E-5 7 6.460334354604523E-5 6.460334354604523E-5 0.0 0.0 3.2301671773022613E-5 8 6.460334354604523E-5 6.460334354604523E-5 0.0 0.0 3.2301671773022613E-5 9 6.460334354604523E-5 6.460334354604523E-5 0.0 0.0 3.2301671773022613E-5 10-11 6.460334354604523E-5 6.460334354604523E-5 0.0 0.0 3.2301671773022613E-5 12-13 9.690501531906784E-5 6.460334354604523E-5 0.0 0.0 6.460334354604523E-5 14-15 1.2920668709209045E-4 8.075417943255653E-5 0.0 0.0 9.690501531906784E-5 16-17 1.2920668709209045E-4 9.690501531906784E-5 0.0 0.0 9.690501531906784E-5 18-19 1.2920668709209045E-4 9.690501531906784E-5 0.0 3.2301671773022613E-5 9.690501531906784E-5 20-21 1.2920668709209045E-4 9.690501531906784E-5 0.0 3.2301671773022613E-5 9.690501531906784E-5 22-23 1.6150835886511307E-4 9.690501531906784E-5 0.0 8.075417943255653E-5 1.1305585120557915E-4 24-25 1.9381003063813568E-4 9.690501531906784E-5 0.0 1.2920668709209045E-4 1.4535752297860176E-4 26-27 1.9381003063813568E-4 9.690501531906784E-5 0.0 2.907150459572035E-4 1.6150835886511307E-4 28-29 2.261117024111583E-4 9.690501531906784E-5 0.0 0.0014535752297860176 1.6150835886511307E-4 30-31 2.261117024111583E-4 9.690501531906784E-5 0.0 0.0060727142933282505 1.7765919475162437E-4 32-33 2.261117024111583E-4 9.690501531906784E-5 0.0 0.016280042573603395 1.9381003063813568E-4 34-35 2.261117024111583E-4 9.690501531906784E-5 0.0 0.03002440391302452 1.9381003063813568E-4 36-37 2.261117024111583E-4 9.690501531906784E-5 0.0 0.052361009944069656 1.9381003063813568E-4 38-39 3.2301671773022613E-4 9.690501531906784E-5 0.0 0.08807050808914615 1.9381003063813568E-4 40-41 3.2301671773022613E-4 1.1305585120557915E-4 0.0 0.1271232292627305 1.9381003063813568E-4 42-43 3.8762006127627136E-4 1.2920668709209045E-4 0.0 0.17278164231389795 1.9381003063813568E-4 44-45 4.845250765953392E-4 1.2920668709209045E-4 0.0 0.23131227156661494 2.09960866524647E-4 46-47 5.006759124818505E-4 1.2920668709209045E-4 0.0 0.29854820136216154 2.422625382976696E-4 48-49 5.168267483683618E-4 1.2920668709209045E-4 0.0 0.3704678735647964 2.584133741841809E-4 50-51 5.168267483683618E-4 1.6150835886511307E-4 0.0 0.4472973998769306 2.584133741841809E-4 52-53 5.168267483683618E-4 1.6150835886511307E-4 0.0 0.5315078581892005 2.745642100706922E-4 54-55 5.168267483683618E-4 1.6150835886511307E-4 0.0 0.6313523256396135 2.907150459572035E-4 56-57 5.491284201413844E-4 1.6150835886511307E-4 0.0 0.7524351422807887 3.0686588184371483E-4 58-59 5.491284201413844E-4 1.6150835886511307E-4 0.0 0.8826108795260699 3.2301671773022613E-4 60-61 5.491284201413844E-4 1.6150835886511307E-4 0.0 1.0211042972529043 3.2301671773022613E-4 62-63 5.491284201413844E-4 1.6150835886511307E-4 0.0 1.170063456634198 3.5531838950324875E-4 64-65 5.491284201413844E-4 1.6150835886511307E-4 0.0 1.3423767247073872 3.5531838950324875E-4 66-67 5.491284201413844E-4 1.6150835886511307E-4 0.0 1.5452796759496288 3.5531838950324875E-4 68-69 5.81430091914407E-4 1.6150835886511307E-4 0.0 1.7703254231922774 3.5531838950324875E-4 70-71 5.81430091914407E-4 1.6150835886511307E-4 0.0 2.0175301172712192 3.5531838950324875E-4 72-73 5.81430091914407E-4 1.6150835886511307E-4 0.0 2.272390307560368 4.360725689358053E-4 74-75 5.81430091914407E-4 1.6150835886511307E-4 0.0 2.560456616432184 4.522234048223166E-4 76-77 5.81430091914407E-4 1.6150835886511307E-4 0.0 2.89106422702907 4.522234048223166E-4 78-79 5.81430091914407E-4 1.6150835886511307E-4 0.0 3.249273616156004 4.522234048223166E-4 80-81 6.137317636874297E-4 1.6150835886511307E-4 0.0 3.629448142088594 4.522234048223166E-4 82-83 6.137317636874297E-4 1.6150835886511307E-4 0.0 4.027889263408827 4.845250765953392E-4 84-85 6.137317636874297E-4 1.6150835886511307E-4 0.0 4.455514945175988 4.845250765953392E-4 86-87 6.460334354604523E-4 1.6150835886511307E-4 0.0 4.929768090147506 5.006759124818505E-4 88-89 6.460334354604523E-4 1.6150835886511307E-4 0.0 5.445722693377996 5.168267483683618E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3170 0.0 47.96193 1 GTATCAA 5760 0.0 39.84103 1 ATCAACG 6605 0.0 33.37136 3 TCAACGC 6690 0.0 32.94736 4 TATCAAC 7010 0.0 31.985987 2 CAACGCA 6965 0.0 31.57727 5 AACGCAG 7245 0.0 30.551125 6 GTACATG 8180 0.0 26.486065 1 ACGCAGA 8435 0.0 26.015762 7 ACATGGG 8390 0.0 24.686146 3 CGCAGAG 8885 0.0 24.644281 8 TACATGG 8640 0.0 24.577038 2 GTGGTAT 1560 0.0 21.319616 1 GCAGAGT 10265 0.0 21.14608 9 CATGGGG 7005 0.0 21.0224 4 TATAAGC 1670 0.0 19.342882 3 AGAGTAC 9705 0.0 18.54882 10-11 CTATAAG 1740 0.0 18.292006 2 CCTATAA 1700 0.0 18.166462 1 CAGAGTA 10085 0.0 17.755713 10-11 >>END_MODULE