FastQCFastQC Report
Fri 27 May 2016
SRR940222_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940222_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165650
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41800.1320423925576106No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28600.045.4838181
GTATCAA49500.039.952661
TCAACGC59050.034.9027334
ATCAACG59600.034.500963
CAACGCA62900.032.8418925
AACGCAG65350.031.6096366
TATCAAC60700.031.5522922
ACGCAGA75850.027.1725647
GTACATG80000.025.800741
CGCAGAG80450.025.4425938
ACATGGG85300.024.6629093
TACATGG85050.024.155512
CATGGGG71750.021.444344
GCAGAGT96300.021.0074279
GTGGTAT14800.020.1086831
CAGAGTA89600.018.65698210-11
AGAGTAC87150.018.39132910-11
GTATAGG7400.018.1626821
GAGTACT52250.017.17946812-13
ATACCGT3059.318501E-817.1265036
Produced by FastQC (version 0.11.5)