##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940222_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3165650 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.945699619351476 33.0 31.0 34.0 30.0 34.0 2 30.125779539746972 33.0 31.0 34.0 16.0 34.0 3 31.50759622826276 34.0 31.0 34.0 27.0 34.0 4 35.60118237960609 37.0 35.0 37.0 33.0 37.0 5 35.91015841928198 37.0 35.0 37.0 35.0 37.0 6 36.1416603225246 37.0 37.0 37.0 35.0 37.0 7 36.20638289134933 37.0 37.0 37.0 35.0 37.0 8 36.2410658158672 37.0 37.0 37.0 35.0 37.0 9 38.08343373398828 39.0 39.0 39.0 37.0 39.0 10-11 38.030517587225376 39.0 39.0 39.0 37.0 39.0 12-13 37.97006854832341 39.0 39.0 39.0 37.0 39.0 14-15 39.469336945019194 41.0 40.0 41.0 37.0 41.0 16-17 39.41438867215264 41.0 40.0 41.0 37.0 41.0 18-19 39.359109029741134 41.0 40.0 41.0 37.0 41.0 20-21 39.2770184638226 41.0 40.0 41.0 37.0 41.0 22-23 39.172983589468195 41.0 39.0 41.0 36.5 41.0 24-25 39.06858480880704 41.0 39.0 41.0 36.0 41.0 26-27 38.944698087280656 40.0 39.0 41.0 36.0 41.0 28-29 38.79131126308973 40.0 39.0 41.0 36.0 41.0 30-31 38.63227125550834 40.0 38.0 41.0 35.0 41.0 32-33 38.70326441647055 40.0 39.0 41.0 35.0 41.0 34-35 38.74910729234122 40.5 38.5 41.0 35.0 41.0 36-37 38.8692840332949 41.0 39.0 41.0 35.0 41.0 38-39 38.80974649755974 41.0 39.0 41.0 35.0 41.0 40-41 38.69298864372246 41.0 39.0 41.0 35.0 41.0 42-43 38.44188965931167 40.0 39.0 41.0 35.0 41.0 44-45 38.39438709269818 40.0 38.0 41.0 35.0 41.0 46-47 38.20700124776902 40.0 38.0 41.0 34.5 41.0 48-49 38.0108101021907 40.0 38.0 41.0 34.0 41.0 50-51 37.09769004785747 39.0 37.0 40.5 32.5 40.5 52-53 37.19288313616477 39.5 37.0 40.5 33.0 41.0 54-55 37.5028728697108 40.0 37.0 41.0 33.0 41.0 56-57 37.342703236302185 40.0 37.0 41.0 33.0 41.0 58-59 37.090271350275614 39.5 36.0 41.0 33.0 41.0 60-61 36.784874512343436 39.0 35.5 41.0 32.5 41.0 62-63 36.44496864782904 39.0 35.0 41.0 32.0 41.0 64-65 36.05679797198047 38.0 35.0 40.0 31.5 41.0 66-67 35.68332111888553 37.0 35.0 40.0 31.0 41.0 68-69 35.29778544690664 37.0 35.0 39.0 31.0 41.0 70-71 34.87754315859302 36.0 35.0 39.0 31.0 41.0 72-73 34.457574905627595 35.5 34.5 38.5 30.0 40.0 74-75 34.03182047920649 35.0 34.0 37.0 30.0 39.0 76-77 33.57870626885473 35.0 34.0 37.0 29.0 39.0 78-79 33.16091971632998 35.0 34.0 36.0 29.0 38.5 80-81 32.841403661175434 35.0 34.0 36.0 28.5 37.0 82-83 32.51557421066764 35.0 33.5 35.5 27.5 37.0 84-85 32.23872143161752 35.0 33.0 35.0 27.0 36.5 86-87 31.98175382622842 35.0 33.0 35.0 26.0 36.0 88-89 31.71858544058882 35.0 33.0 35.0 25.5 36.0 90-91 31.491204491968475 35.0 33.0 35.0 25.0 35.5 92-93 31.243830177056843 35.0 32.5 35.0 24.5 35.0 94-95 30.945715887732376 34.5 32.0 35.0 23.5 35.0 96-97 30.624076729897496 34.0 32.0 35.0 19.5 35.0 98-99 30.22624468908439 34.0 31.5 35.0 10.0 35.0 100-101 28.383503861766144 32.5 28.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6794766969076029 1101 2 1.9001416893546796 1101 3 1.0288613237527677 1101 4 0.5595553023664195 1101 5 0.3855221944245528 1101 6 0.348872793094408 1101 7 0.2739909226733914 1101 8 0.2462450482101488 1101 9 0.2782546325932955 1101 10-11 0.2769331377717279 1101 12-13 0.27664853968967407 1101 14-15 0.41139100722423194 1101 16-17 0.40637838701325535 1101 18-19 0.4554270694469196 1101 20-21 0.3533875850810375 1101 22-23 0.3880856017566998 1101 24-25 0.38748974299392813 1101 26-27 0.4274285792763024 1101 28-29 0.4357228079878581 1101 30-31 0.5329626195367254 1101 32-33 0.5266108506633458 1101 34-35 0.5249002867947254 1101 36-37 0.5811902229575665 1101 38-39 0.557933032274093 1101 40-41 0.5690699300785695 1101 42-43 0.6267072776536153 1101 44-45 0.5359375379356166 1101 46-47 0.46772293337745907 1101 48-49 0.45750729705062554 1101 50-51 0.36270699834178544 1101 52-53 0.41641435175423425 1101 54-55 0.5321619192668479 1101 56-57 0.56121476731483 1101 58-59 0.5178396499847793 1101 60-61 0.6166748592030089 1101 62-63 0.7031055922814744 1101 64-65 0.6876421304100049 1101 66-67 0.5981115384440088 1101 68-69 0.5477742225759954 1101 70-71 0.5542213757719239 1101 72-73 0.4397685163787415 1101 74-75 0.36236413769231746 1101 76-77 0.41105723983246634 1101 78-79 0.4228296486111631 1101 80-81 0.4913238153533612 1101 82-83 0.4862107230242927 1101 84-85 0.41555401499510936 1101 86-87 0.31587572535912756 1101 88-89 0.34283393554778385 1101 90-91 0.40358848177804063 1101 92-93 0.3909414975221921 1101 94-95 0.26940871152082835 1101 96-97 0.21841639202158802 1101 98-99 0.1397964586677496 1101 100-101 0.18586425741792212 1102 1 0.8389764292871753 1102 2 2.70695361417496 1102 3 1.486553633293024 1102 4 0.7260643356368774 1102 5 0.44931567313455645 1102 6 0.3105594696330769 1102 7 0.23936026288573942 1102 8 0.20641044436045064 1102 9 0.213236081741087 1102 10-11 0.20937675730677086 1102 12-13 0.2875171361382485 1102 14-15 0.31172011548606804 1102 16-17 0.312462355444616 1102 18-19 0.2946032285508622 1102 20-21 0.3308866890090556 1102 22-23 0.32271763087688043 1102 24-25 0.38952410267001625 1102 26-27 0.3956981417529377 1102 28-29 0.41899677154727755 1102 30-31 0.3501068189000094 1102 32-33 0.3643469283953067 1102 34-35 0.3812746618265592 1102 36-37 0.4122760892357604 1102 38-39 0.461561931628637 1102 40-41 0.4091096695498422 1102 42-43 0.5708798909070225 1102 44-45 0.3506010232664636 1102 46-47 0.34400367969732315 1102 48-49 0.4141849191782683 1102 50-51 0.27950935705309377 1102 52-53 0.3486153351752179 1102 54-55 0.3549006828124348 1102 56-57 0.38576657517008783 1102 58-59 0.3884919591598788 1102 60-61 0.4436245004378847 1102 62-63 0.4107474527287138 1102 64-65 0.39392721054718294 1102 66-67 0.33788196384037406 1102 68-69 0.2839779571541925 1102 70-71 0.40456480196682776 1102 72-73 0.33779816699235 1102 74-75 0.37547831936478104 1102 76-77 0.4759181973274238 1102 78-79 0.42141653348055996 1102 80-81 0.4791275669401074 1102 82-83 0.49157358172934806 1102 84-85 0.446010793095013 1102 86-87 0.4231084556736491 1102 88-89 0.45586801126754395 1102 90-91 0.3889122400336724 1102 92-93 0.3738283844095598 1102 94-95 0.30899785650960254 1102 96-97 0.1491702071741905 1102 98-99 0.13968553072154677 1102 100-101 0.14544414379696136 1103 1 0.795308823490533 1103 2 2.6633310804155066 1103 3 1.3915606050623133 1103 4 0.7386590353646412 1103 5 0.4684251094804921 1103 6 0.3386026931630326 1103 7 0.23884553357654426 1103 8 0.2500591132569454 1103 9 0.22393244028593529 1103 10-11 0.21083041356261134 1103 12-13 0.22264596076915666 1103 14-15 0.29455996169185994 1103 16-17 0.3202767060490359 1103 18-19 0.3518524123538569 1103 20-21 0.3713853540683374 1103 22-23 0.2909208590314165 1103 24-25 0.35423685038211516 1103 26-27 0.3942669107735881 1103 28-29 0.38090746711955603 1103 30-31 0.4642044661950351 1103 32-33 0.4516779317885522 1103 34-35 0.43164310635068404 1103 36-37 0.3966244090399158 1103 38-39 0.46637788433909577 1103 40-41 0.509248329740899 1103 42-43 0.6577426766756389 1103 44-45 0.501777010302547 1103 46-47 0.474509707999232 1103 48-49 0.5553241276534919 1103 50-51 0.4216987882916001 1103 52-53 0.43617020373596205 1103 54-55 0.5026125460273292 1103 56-57 0.6015649554339006 1103 58-59 0.5705935652637208 1103 60-61 0.5614873239695655 1103 62-63 0.5960328943831854 1103 64-65 0.5235353418527708 1103 66-67 0.5032289537425783 1103 68-69 0.5264773112508578 1103 70-71 0.35568295947510364 1103 72-73 0.4829820647800389 1103 74-75 0.4693834171450817 1103 76-77 0.4200217283505552 1103 78-79 0.5829042717269814 1103 80-81 0.5210593455945443 1103 82-83 0.39408298677956566 1103 84-85 0.3750327442845389 1103 86-87 0.40357415723763523 1103 88-89 0.46233069909584756 1103 90-91 0.3362839920922873 1103 92-93 0.2614369407433479 1103 94-95 0.19690779748516363 1103 96-97 0.12103163469087974 1103 98-99 0.11748040954144301 1103 100-101 0.1880152920010154 1104 1 0.7279028153993288 1104 2 2.579066707736917 1104 3 1.341789163725199 1104 4 0.6874931643033833 1104 5 0.42904418767043495 1104 6 0.25020787034685554 1104 7 0.15028015026189934 1104 8 0.14995887098942973 1104 9 0.09281839704841133 1104 10-11 0.154611741983679 1104 12-13 0.20594573353960755 1104 14-15 0.1773007582332582 1104 16-17 0.23835254926834892 1104 18-19 0.16834797783514688 1104 20-21 0.1820620072764214 1104 22-23 0.14753291559717496 1104 24-25 0.2220272380394377 1104 26-27 0.20727428621315624 1104 28-29 0.1490254242431206 1104 30-31 0.20497388283147444 1104 32-33 0.269637312133149 1104 34-35 0.2634994783531184 1104 36-37 0.23529452105758253 1104 38-39 0.21651814647930223 1104 40-41 0.23329798931972334 1104 42-43 0.4008842079291739 1104 44-45 0.2741237176983091 1104 46-47 0.2269157556926018 1104 48-49 0.259800451046182 1104 50-51 0.20317829428676504 1104 52-53 0.3087568459194614 1104 54-55 0.40880861183025985 1104 56-57 0.45251146312632073 1104 58-59 0.4956489892882985 1104 60-61 0.44179687391444844 1104 62-63 0.4818695484201925 1104 64-65 0.4582173436297694 1104 66-67 0.3825380339950897 1104 68-69 0.39329039376188035 1104 70-71 0.3013207608027102 1104 72-73 0.3051623292160812 1104 74-75 0.3360683080346476 1104 76-77 0.5086998421727813 1104 78-79 0.47482647443987247 1104 80-81 0.4798056760212219 1104 82-83 0.4914717836673006 1104 84-85 0.41431297898099473 1104 86-87 0.47489703509667436 1104 88-89 0.5213277599652208 1104 90-91 0.4519159811260671 1104 92-93 0.27504640993238993 1104 94-95 0.2364399669637116 1104 96-97 0.26735149356400356 1104 98-99 0.05471067413480668 1104 100-101 0.0012991772563317738 1105 1 0.6816778976136355 1105 2 2.4460966141606555 1105 3 1.2504827453377452 1105 4 0.6863494285005132 1105 5 0.4159516042273239 1105 6 0.30773483931633905 1105 7 0.20470111555835047 1105 8 0.16812514249780008 1105 9 0.10356764436426147 1105 10-11 0.11280994548263124 1105 12-13 0.13785883001019528 1105 14-15 0.0647941787520594 1105 16-17 0.11218151844260404 1105 18-19 0.07138453319901572 1105 20-21 0.09827924422143042 1105 22-23 0.13349769800804978 1105 24-25 0.050969064167802 1105 26-27 0.0746148461073659 1105 28-29 0.05806019364160164 1105 30-31 0.14214456360120664 1105 32-33 0.16499254889038895 1105 34-35 0.2052959220559103 1105 36-37 0.2033116576588725 1105 38-39 0.09860582488997238 1105 40-41 0.1607310379210034 1105 42-43 0.2908321094835671 1105 44-45 0.11580472575766265 1105 46-47 0.030702616187198828 1105 48-49 0.05170664835456762 1105 50-51 -0.020144060145788956 1105 52-53 0.07550181142152468 1105 54-55 0.15905793115398836 1105 56-57 0.20509923308684108 1105 58-59 0.15873499856053286 1105 60-61 0.17135916795384531 1105 62-63 0.13927405088821843 1105 64-65 0.22182210702546712 1105 66-67 0.07890665954518283 1105 68-69 0.11701999630811599 1105 70-71 0.033910041017030323 1105 72-73 0.11097358827495185 1105 74-75 0.12687665066811604 1105 76-77 0.19953304770451297 1105 78-79 0.16602391452781973 1105 80-81 0.16630943423788125 1105 82-83 0.1968753273047099 1105 84-85 0.12662827026881018 1105 86-87 0.2062286201324568 1105 88-89 0.18041164978424362 1105 90-91 0.06939009546099228 1105 92-93 0.09968666790327774 1105 94-95 -0.15653486766243674 1105 96-97 -0.24221588988200082 1105 98-99 -0.4278315566821007 1105 100-101 -0.31481287295461513 1106 1 0.7375659968382315 1106 2 2.584246152721736 1106 3 1.3830990084072958 1106 4 0.751066315295148 1106 5 0.44198388079235684 1106 6 0.2777663091773306 1106 7 0.19561267012326056 1106 8 0.16163149004019317 1106 9 0.15335042249333952 1106 10-11 0.1602703508093981 1106 12-13 0.17390909985689973 1106 14-15 0.1691179233714024 1106 16-17 0.09654380208730373 1106 18-19 0.12449606843664185 1106 20-21 0.18291636395186117 1106 22-23 0.1369597503555795 1106 24-25 0.13298150715756663 1106 26-27 0.06840396022447237 1106 28-29 0.02642967459896539 1106 30-31 0.15879312844530347 1106 32-33 0.14887979099194126 1106 34-35 0.1256284878999807 1106 36-37 0.12923322249665858 1106 38-39 0.20889849669798366 1106 40-41 0.21690936153117235 1106 42-43 0.206695976074883 1106 44-45 0.2155532609805917 1106 46-47 0.15293072646196748 1106 48-49 0.07840503347593142 1106 50-51 0.10553811946770253 1106 52-53 0.17027015711101257 1106 54-55 0.39547688995956065 1106 56-57 0.3966393970746438 1106 58-59 0.28896481467822355 1106 60-61 0.31860986174033457 1106 62-63 0.3624940500058855 1106 64-65 0.3457852612382908 1106 66-67 0.42071824583205597 1106 68-69 0.3603348041591943 1106 70-71 0.36128020683252515 1106 72-73 0.3688772396611526 1106 74-75 0.3026143437046187 1106 76-77 0.3505759857878772 1106 78-79 0.4477223941848081 1106 80-81 0.44780371693302357 1106 82-83 0.43297795938674 1106 84-85 0.2871354329184115 1106 86-87 0.3419962809638264 1106 88-89 0.45980120170635885 1106 90-91 0.2946604673641602 1106 92-93 0.3401336848839769 1106 94-95 0.352446857549797 1106 96-97 0.3311405031425245 1106 98-99 0.18592883961126105 1106 100-101 0.1581917424849415 1107 1 0.7515091124509077 1107 2 2.4978235837124068 1107 3 1.313959186950715 1107 4 0.658668951878731 1107 5 0.370330605643737 1107 6 0.23758373586403536 1107 7 0.16707069353824266 1107 8 0.11546924990847174 1107 9 0.09914139503171526 1107 10-11 0.10510825938773394 1107 12-13 0.1431515464773554 1107 14-15 0.10161599343972227 1107 16-17 0.17122604219871818 1107 18-19 0.10325915606340885 1107 20-21 0.1208969626189571 1107 22-23 0.18379798680659576 1107 24-25 0.12447057189313426 1107 26-27 0.19397711567418696 1107 28-29 0.23671619776296637 1107 30-31 0.17801641532323487 1107 32-33 0.1532115169370698 1107 34-35 0.13269698639029315 1107 36-37 0.10859490615840173 1107 38-39 0.07508568763610413 1107 40-41 0.14796245711113443 1107 42-43 0.23550623980937502 1107 44-45 0.137519157146464 1107 46-47 0.11071935212221717 1107 48-49 0.13935270733092153 1107 50-51 -0.031875147803852144 1107 52-53 0.07564168608892174 1107 54-55 0.31070540858917894 1107 56-57 0.3800979666057387 1107 58-59 0.3589773560619989 1107 60-61 0.3433216662226499 1107 62-63 0.4097678089748058 1107 64-65 0.37078382742974014 1107 66-67 0.4042292445393443 1107 68-69 0.3981904965313845 1107 70-71 0.3964038975623083 1107 72-73 0.4266913439723794 1107 74-75 0.4405028740990744 1107 76-77 0.4296310120608311 1107 78-79 0.47268061071984135 1107 80-81 0.48837491580506054 1107 82-83 0.5208402751931018 1107 84-85 0.4473841695173775 1107 86-87 0.42827993549565946 1107 88-89 0.3526761629427746 1107 90-91 0.350392174124174 1107 92-93 0.27917037870926364 1107 94-95 0.09639489125267531 1107 96-97 0.3852304102977122 1107 98-99 0.4620184960402831 1107 100-101 0.4244115176739136 1108 1 0.7298724284366465 1108 2 2.547410557526522 1108 3 1.3334583009708219 1108 4 0.6755227685382934 1108 5 0.40654141916131437 1108 6 0.27692054569121893 1108 7 0.17650273930171068 1108 8 0.12181744044312381 1108 9 0.1518331493009697 1108 10-11 0.15325531606574572 1108 12-13 0.15780661591858802 1108 14-15 0.22950995950540687 1108 16-17 0.2526689121167607 1108 18-19 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2323.0 8 2331.0 9 2325.0 10 2699.0 11 3288.0 12 4109.0 13 5095.0 14 5572.0 15 6792.0 16 9067.0 17 12215.0 18 11035.0 19 7622.0 20 7464.0 21 7847.0 22 8253.0 23 8714.0 24 9645.0 25 10994.0 26 13307.0 27 15941.0 28 19827.0 29 24744.0 30 31946.0 31 42553.0 32 57810.0 33 83595.0 34 130132.0 35 220972.0 36 440105.0 37 901895.0 38 906038.0 39 125114.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.6043252357878 17.920535895424802 13.384587381632096 25.09055148715531 2 17.803359275058416 20.42338300750463 36.79222291251096 24.981034804926 3 18.970709049544997 22.890703993933265 28.949222699696662 29.189364256825073 4 13.701304713126476 15.899767343906657 35.23208890919587 35.166839033771 5 15.306066989105215 35.73133575636235 33.728134122302926 15.234463132229509 6 34.39368138377695 34.00379116973383 17.039222810204567 14.563304636284654 7 30.160112327600263 30.542510208440493 20.578459337484535 18.71891812647471 8 27.06159541178195 33.79422150436528 19.789375969991557 19.35480711386121 9 26.77447176869029 15.697663397866856 19.482984127786484 38.04488070565637 10-11 25.883778130875694 25.15380373495036 27.156368091468895 21.80605004270505 12-13 26.463306491045614 22.29501323050785 27.285906147995583 23.955774130450962 14-15 23.620106585044983 23.816193875087986 24.68878468531884 27.874914854548187 16-17 24.114775387940433 26.86672733419911 25.374669409344698 23.643827868515764 18-19 24.057520748068022 25.941167883027155 26.74247748237394 23.258833886530883 20-21 24.358517127515665 25.418673395168934 26.687457266736626 23.53535221057878 22-23 24.4272459712408 25.097120603977345 26.503528699478167 23.972104725303687 24-25 24.364849269480697 25.331819441193094 26.381466068060067 23.921865221266142 26-27 24.022441416308336 25.649704751463602 26.39356045407298 23.934293378155083 28-29 24.049770588791034 25.608885248895604 26.427551968645503 23.913792193667867 30-31 24.096541234509235 25.4539844949386 26.31582439476896 24.13364987578321 32-33 23.970831035828436 25.476898818674332 26.353247500130344 24.199022645366888 34-35 24.41831696464401 25.539373644061424 26.060831362160176 23.981478029134394 36-37 24.20866922583762 25.368195481143204 26.130559427102966 24.29257586591621 38-39 24.092656249333587 25.551394596889775 26.446041956301038 23.9099071974756 40-41 24.37480056741532 25.232273800008215 26.405679840011626 23.98724579256484 42-43 24.347101098548368 25.330730288872854 26.106636152136247 24.215532460442528 44-45 24.18508117316779 25.555436723127517 25.982601563416996 24.276880540287696 46-47 24.209029949499424 25.507853108710115 25.88852277328071 24.39459416850975 48-49 24.313554334196752 25.60849163451359 25.79795073305223 24.280003298237432 50-51 24.043650149169977 25.69810663773214 25.897484427362343 24.360758785735545 52-53 24.420512801070164 25.514987397026218 25.727569771314002 24.33693003058962 54-55 24.180927388167973 25.35284780531698 25.924708951111874 24.54151585540317 56-57 24.14311979245491 25.83962901726035 25.885790169285993 24.131461020998742 58-59 24.263061583433164 25.488707942411477 25.912212433783484 24.336018040371872 60-61 24.30024476544128 25.28066510988221 26.175179008622557 24.243911116053955 62-63 24.29192517832308 25.457469062728933 26.064373411349074 24.186232347598917 64-65 24.156626458440133 25.425590820802324 25.96808987095505 24.44969284980249 66-67 24.0153504278743 25.713009302796557 25.82153587371867 24.450104395610474 68-69 23.8775453786484 25.41340163924715 26.18871483307782 24.52033814902663 70-71 23.848042708111844 25.44176147897405 26.05066548966814 24.659530323245964 72-73 24.075442069293462 25.42628702868454 25.93381559553168 24.564455306490316 74-75 23.889685515433527 25.51497219039578 25.960523064497682 24.634819229673017 76-77 24.080943514208737 25.368666877945834 25.99275668444213 24.557632923403297 78-79 23.875024230611917 25.46207204236846 26.00166229501929 24.661241432000335 80-81 23.8128443300412 25.478219273604015 26.17328531261858 24.5356510837362 82-83 23.900059698531106 25.458129750389368 25.97216269658534 24.66964785449419 84-85 23.85442146522586 25.342313406098167 25.9231514380986 24.880113690577367 86-87 23.913679193938073 25.262439813850087 26.22448610424098 24.59939488797086 88-89 23.7896027324977 25.19321488718336 26.10604858967351 24.91113379064543 90-91 23.710619055773595 25.38651886892007 26.23290467184363 24.669957403462696 92-93 23.849347659114496 25.26133216239418 26.255087241340135 24.63423293715119 94-95 23.794971609438033 25.050733837039484 26.187067482285237 24.967227071237243 96-97 23.994496937483117 24.976895388229394 26.093377246979806 24.935230427307683 98-99 23.66712401522528 24.92352584124736 26.151980930461942 25.257369213065417 100-101 23.634942833370847 24.891113440543418 26.445145451832598 25.028798274253138 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 210.0 1 164.0 2 155.0 3 202.0 4 197.5 5 214.0 6 331.5 7 640.0 8 1118.0 9 1449.5 10 1467.0 11 1420.0 12 1465.0 13 1584.5 14 1710.0 15 1746.5 16 1758.0 17 1830.0 18 1904.5 19 2049.5 20 2275.5 21 2601.0 22 3032.5 23 3579.5 24 4274.5 25 5308.0 26 6759.5 27 8650.0 28 10947.5 29 13602.5 30 17214.0 31 21321.5 32 25341.0 33 29987.5 34 35279.5 35 40283.5 36 45744.5 37 52133.0 38 58109.5 39 61931.0 40 65654.5 41 72908.5 42 80926.5 43 89745.0 44 98789.0 45 105100.0 46 111044.5 47 119317.5 48 131435.5 49 141623.0 50 146129.5 51 148248.5 52 146850.0 53 146510.0 54 146623.5 55 141370.5 56 133829.5 57 123318.0 58 110865.0 59 97126.0 60 80680.5 61 63681.5 62 49758.5 63 38683.0 64 29102.0 65 21425.5 66 15241.5 67 11391.0 68 8107.5 69 5381.0 70 3976.0 71 2921.0 72 2180.5 73 1596.5 74 1164.0 75 883.5 76 629.5 77 396.5 78 248.0 79 169.0 80 108.5 81 83.5 82 79.0 83 59.0 84 50.0 85 49.5 86 35.5 87 31.5 88 35.5 89 28.0 90 24.0 91 23.0 92 21.5 93 22.0 94 20.5 95 15.5 96 11.0 97 9.5 98 9.0 99 9.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03639063067616445 2 1.102933046925592 3 0.026850725759322732 4 0.028209056591853174 5 0.03209451455467281 6 0.01263563565144599 7 0.019648413437998516 8 0.021291046072686493 9 0.011687962977587542 10-11 0.010534961224393095 12-13 0.025445011293099364 14-15 0.015099584603477958 16-17 0.02716661665060888 18-19 0.032157692732930045 20-21 0.04420893023549666 22-23 0.0324735836242162 24-25 0.009445137649455877 26-27 0.016426326346879787 28-29 0.02555557310504952 30-31 0.01955364617061267 32-33 0.03111525279168575 34-35 0.0274193293636378 36-37 0.025997820352850125 38-39 0.012019648413438 40-41 0.013267417434018292 42-43 0.01206703204713092 44-45 0.01881130257609022 46-47 0.013851815582897666 48-49 0.010203275788542637 50-51 0.025397627659406445 52-53 0.016552682703394248 54-55 0.017247642664223777 56-57 0.020564497022728348 58-59 0.02364443321276831 60-61 0.01829008260546807 62-63 0.025855669451771358 64-65 0.021085716993350496 66-67 0.016631655426215787 68-69 0.015083790058913651 70-71 0.01696334086206624 72-73 0.022270307835673558 74-75 0.02893560564181132 76-77 0.025302860392020596 78-79 0.02364443321276831 80-81 0.01980635888364159 82-83 0.018337466239160993 84-85 0.017942602625053306 86-87 0.024686873154012603 88-89 0.024639489520319684 90-91 0.01907980983368345 92-93 0.03912308688578965 94-95 0.05795018400644418 96-97 0.07330248132295106 98-99 0.07865683193025129 100-101 0.07158087596544153 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3165650.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.0657046134794 #Duplication Level Percentage of deduplicated Percentage of total 1 80.84475265492254 46.134627745556145 2 10.270389415238423 11.721740172708024 3 3.3463371185833033 5.728832566385897 4 1.639498535787696 3.742365566299706 5 0.9419918163238249 2.6877713369325167 6 0.6219769592992799 2.129613206145768 7 0.4201351766290202 1.678271692107056 8 0.29190091891390657 1.3326025292115353 9 0.2331762428759828 1.1975729938957587 >10 1.2468858457560046 12.874756635675205 >50 0.08811346615379848 3.46657502587537 >100 0.051070217006619095 5.333466674502008 >500 0.0029272371720368735 1.1623430522115141 >1k 8.443953375829989E-4 0.8094608024934967 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4180 0.1320423925576106 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.476726738584493E-5 2 0.0 0.0 0.0 0.0 9.476726738584493E-5 3 0.0 0.0 0.0 0.0 9.476726738584493E-5 4 0.0 0.0 0.0 0.0 9.476726738584493E-5 5 3.1589089128614976E-5 0.0 0.0 0.0 9.476726738584493E-5 6 3.1589089128614976E-5 0.0 0.0 0.0 9.476726738584493E-5 7 3.1589089128614976E-5 0.0 0.0 0.0 9.476726738584493E-5 8 3.1589089128614976E-5 0.0 0.0 0.0 9.476726738584493E-5 9 3.1589089128614976E-5 0.0 0.0 0.0 9.476726738584493E-5 10-11 3.1589089128614976E-5 0.0 0.0 0.0 9.476726738584493E-5 12-13 3.1589089128614976E-5 0.0 0.0 0.0 1.263563565144599E-4 14-15 3.1589089128614976E-5 0.0 0.0 0.0 1.421509010787674E-4 16-17 6.317817825722995E-5 0.0 0.0 1.5794544564307488E-5 1.5794544564307487E-4 18-19 6.317817825722995E-5 1.5794544564307488E-5 0.0 3.1589089128614976E-5 1.5794544564307487E-4 20-21 6.317817825722995E-5 3.1589089128614976E-5 0.0 3.1589089128614976E-5 2.2112362390030484E-4 22-23 6.317817825722995E-5 3.1589089128614976E-5 0.0 4.738363369292247E-5 2.2112362390030484E-4 24-25 1.263563565144599E-4 3.1589089128614976E-5 0.0 7.897272282153745E-5 2.2112362390030484E-4 26-27 1.263563565144599E-4 3.1589089128614976E-5 0.0 1.7373999020738236E-4 2.527127130289198E-4 28-29 1.421509010787674E-4 3.1589089128614976E-5 0.0 0.0013741253770947514 2.685072575932273E-4 30-31 1.5794544564307487E-4 3.1589089128614976E-5 0.0 0.005843981488793771 2.843018021575348E-4 32-33 1.5794544564307487E-4 3.1589089128614976E-5 0.0 0.015289119138249648 2.843018021575348E-4 34-35 1.5794544564307487E-4 3.1589089128614976E-5 0.0 0.028904016552682703 2.843018021575348E-4 36-37 1.8953453477168987E-4 3.1589089128614976E-5 0.0 0.052027229794828864 3.0009634672184223E-4 38-39 2.2112362390030484E-4 6.317817825722995E-5 0.0 0.0883862713818647 3.1589089128614974E-4 40-41 2.2112362390030484E-4 9.476726738584493E-5 0.0 0.12695654920790359 3.4747998041476477E-4 42-43 2.685072575932273E-4 9.476726738584493E-5 0.0 0.17217633029551593 3.4747998041476477E-4 44-45 2.843018021575348E-4 9.476726738584493E-5 0.0 0.23060035063888934 3.632745249790723E-4 46-47 3.158908912861498E-4 9.476726738584493E-5 0.0 0.3007755121381075 3.7906906954337973E-4 48-49 3.4747998041476477E-4 9.476726738584493E-5 0.0 0.37455182979798773 4.106581586719947E-4 50-51 3.4747998041476477E-4 9.476726738584493E-5 0.0 0.4502708764392779 4.106581586719947E-4 52-53 3.4747998041476477E-4 9.476726738584493E-5 0.0 0.5331132626790707 4.106581586719947E-4 54-55 3.4747998041476477E-4 9.476726738584493E-5 0.0 0.6307393426310552 4.106581586719947E-4 56-57 4.4224724780060967E-4 9.476726738584493E-5 0.0 0.7505725522404562 4.106581586719947E-4 58-59 4.4224724780060967E-4 1.263563565144599E-4 0.0 0.8833414938480249 4.264527032363022E-4 60-61 4.4224724780060967E-4 1.263563565144599E-4 0.0 1.0219070333106943 4.4224724780060967E-4 62-63 4.4224724780060967E-4 1.263563565144599E-4 0.0 1.172539604820495 4.4224724780060967E-4 64-65 4.4224724780060967E-4 1.263563565144599E-4 0.0 1.3459794986811555 4.4224724780060967E-4 66-67 4.4224724780060967E-4 1.263563565144599E-4 0.0 1.550187165353087 4.4224724780060967E-4 68-69 4.4224724780060967E-4 1.263563565144599E-4 0.0 1.777833936158451 4.4224724780060967E-4 70-71 4.5804179236491713E-4 1.263563565144599E-4 0.0 2.0221597460237235 4.4224724780060967E-4 72-73 4.896308814935322E-4 1.263563565144599E-4 0.0 2.275772748092809 4.7383633692922464E-4 74-75 5.054254260578396E-4 1.263563565144599E-4 0.0 2.56196989559806 4.7383633692922464E-4 76-77 5.054254260578396E-4 1.263563565144599E-4 0.0 2.889975202565034 4.7383633692922464E-4 78-79 5.054254260578396E-4 1.263563565144599E-4 0.0 3.251749245810497 5.054254260578396E-4 80-81 6.001926934436846E-4 1.263563565144599E-4 0.0 3.6331243188602658 5.054254260578396E-4 82-83 6.001926934436846E-4 1.263563565144599E-4 0.0 4.037117179726122 5.054254260578396E-4 84-85 6.001926934436846E-4 1.263563565144599E-4 0.0 4.470866962551135 5.054254260578396E-4 86-87 6.001926934436846E-4 1.263563565144599E-4 0.0 4.943755626806501 5.054254260578396E-4 88-89 6.001926934436846E-4 1.263563565144599E-4 0.0 5.466318133716614 5.054254260578396E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2860 0.0 45.483818 1 GTATCAA 4950 0.0 39.95266 1 TCAACGC 5905 0.0 34.902733 4 ATCAACG 5960 0.0 34.50096 3 CAACGCA 6290 0.0 32.841892 5 AACGCAG 6535 0.0 31.609636 6 TATCAAC 6070 0.0 31.552292 2 ACGCAGA 7585 0.0 27.172564 7 GTACATG 8000 0.0 25.80074 1 CGCAGAG 8045 0.0 25.442593 8 ACATGGG 8530 0.0 24.662909 3 TACATGG 8505 0.0 24.15551 2 CATGGGG 7175 0.0 21.44434 4 GCAGAGT 9630 0.0 21.007427 9 GTGGTAT 1480 0.0 20.108683 1 CAGAGTA 8960 0.0 18.656982 10-11 AGAGTAC 8715 0.0 18.391329 10-11 GTATAGG 740 0.0 18.162682 1 GAGTACT 5225 0.0 17.179468 12-13 ATACCGT 305 9.318501E-8 17.126503 6 >>END_MODULE