FastQCFastQC Report
Fri 27 May 2016
SRR940222_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940222_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3165650
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51460.16255745265585267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38880.12281837853205502No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30300.050.483811
GTATCAA56850.042.4494551
TCAACGC67200.034.7075274
ATCAACG68200.034.337923
CAACGCA70650.033.079395
TATCAAC73000.032.5355832
AACGCAG73450.031.8830386
ACGCAGA85350.027.3811997
CGCAGAG91050.025.9270788
GTACATG81000.024.0457171
TACATGG85700.022.3929842
GCAGAGT108250.021.5442169
ACATGGG88150.020.5311893
GTGGTAT16250.019.8789961
AGAGTAC96050.019.78061510-11
CAGAGTA99550.018.91816710-11
CATGGGG72250.018.8692874
GAGTACT60350.018.29924412-13
TATAAGC17200.017.6751443
CTATGCG1901.8675234E-417.4993369
Produced by FastQC (version 0.11.5)