FastQCFastQC Report
Fri 27 May 2016
SRR940221_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940221_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977075
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41620.13980165094933786No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30610.10281904218066391No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.044.4567641
GTATCAA49350.041.9692651
TCAACGC56000.036.5581974
ATCAACG57200.035.7918433
CAACGCA58650.034.824225
AACGCAG61600.033.158736
TATCAAC62000.032.7904662
ACGCAGA71200.028.6211727
CGCAGAG76250.026.7870038
GTACATG74700.025.5009171
TACATGG79800.023.3334372
ACATGGG78750.022.8004843
GCAGAGT91300.022.2673519
CATGGGG64200.019.754764
AGAGTAC83100.019.08993510-11
TTATAGG15250.019.0004023
TAGGACG4500.019.0000844
TATCACG1801.2341148E-418.4723032
GTGGTAT15450.018.4482351
CAGAGTA85950.018.01485610-11
Produced by FastQC (version 0.11.5)