FastQCFastQC Report
Fri 27 May 2016
SRR940221_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940221_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2977075
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49570.1665057145016501No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36160.12146150164171207No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28400.045.672761
GTATCAA53800.045.30521
TCAACGC63750.037.1806144
ATCAACG64350.036.8339423
CAACGCA65200.036.3525245
TATCAAC67800.035.4518552
AACGCAG68250.034.727986
ACGCAGA78900.030.1005637
CGCAGAG83300.028.4535988
GTACATG74700.024.8696171
GCAGAGT99350.023.6178689
TACATGG79150.022.5060062
GTGGTAT15550.022.3051641
ACATGGG79400.021.775953
CATGGGG65850.019.8368684
AGAGTAC88500.019.8310410-11
CAGAGTA90850.019.57948310-11
TGGTATC17400.019.1102732
GAGTACT55200.018.67374812-13
GTCCTAC11250.018.1605431
Produced by FastQC (version 0.11.5)