##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940221_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2977075 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.79175499441566 34.0 31.0 34.0 31.0 34.0 2 32.877112602134645 34.0 31.0 34.0 31.0 34.0 3 32.93477288949724 34.0 31.0 34.0 31.0 34.0 4 36.332470629728846 37.0 37.0 37.0 35.0 37.0 5 36.30151104691686 37.0 37.0 37.0 35.0 37.0 6 36.34640309699957 37.0 37.0 37.0 35.0 37.0 7 36.29623875784117 37.0 37.0 37.0 35.0 37.0 8 36.29239908299253 37.0 37.0 37.0 35.0 37.0 9 38.10404541370305 39.0 39.0 39.0 37.0 39.0 10-11 38.042304275169414 39.0 38.0 39.0 35.0 39.0 12-13 38.014972750098664 39.0 38.0 39.0 35.0 39.0 14-15 39.45436191563867 41.0 39.0 41.0 36.5 41.0 16-17 39.404405330735706 41.0 39.0 41.0 36.0 41.0 18-19 39.361716953721356 41.0 39.0 41.0 36.0 41.0 20-21 39.29134334875675 40.5 39.0 41.0 36.0 41.0 22-23 39.218245257509466 40.0 39.0 41.0 36.0 41.0 24-25 39.09454884408353 40.0 39.0 41.0 36.0 41.0 26-27 38.901877514002834 40.0 38.0 41.0 35.0 41.0 28-29 38.78753390492346 40.0 38.0 41.0 35.0 41.0 30-31 38.851844511811095 40.0 38.0 41.0 35.0 41.0 32-33 38.7506319121957 40.0 38.0 41.0 35.0 41.0 34-35 38.720800618056316 40.0 38.0 41.0 35.0 41.0 36-37 38.8145925111057 40.0 38.0 41.0 35.0 41.0 38-39 38.732494982491204 40.0 38.0 41.0 35.0 41.0 40-41 38.584432538649516 40.0 38.0 41.0 35.0 41.0 42-43 38.41523811123334 40.0 38.0 41.0 34.0 41.0 44-45 38.260238321170945 40.0 38.0 41.0 34.0 41.0 46-47 38.05037394086477 40.0 38.0 41.0 33.5 41.0 48-49 37.825625152204765 40.0 37.5 41.0 33.0 41.0 50-51 37.599809544603346 40.0 37.0 41.0 33.0 41.0 52-53 37.262111972321826 40.0 36.5 41.0 32.0 41.0 54-55 37.108129120025524 39.5 36.0 41.0 32.0 41.0 56-57 36.91234097226304 39.0 36.0 41.0 32.0 41.0 58-59 36.62561289184854 39.0 35.0 40.5 31.0 41.0 60-61 36.26096756044104 38.5 35.0 40.0 31.0 41.0 62-63 35.90863565748259 38.0 35.0 40.0 30.5 41.0 64-65 35.523232703240595 37.0 35.0 40.0 30.0 41.0 66-67 35.08500508049008 36.5 34.0 39.0 29.5 41.0 68-69 34.65912800315746 36.0 34.0 39.0 29.0 40.0 70-71 34.23705533115558 35.5 34.0 38.5 29.0 40.0 72-73 33.79478783705483 35.0 34.0 37.0 28.0 39.5 74-75 33.35357809259088 35.0 33.0 37.0 27.5 39.0 76-77 32.07835156991342 34.0 31.5 35.5 26.0 37.5 78-79 32.75255964327402 35.0 33.0 36.0 27.0 37.5 80-81 32.64728382724654 35.0 33.0 35.5 27.0 37.0 82-83 32.3926315259105 35.0 33.0 35.0 27.0 37.0 84-85 32.10496981097218 35.0 33.0 35.0 26.0 36.0 86-87 31.822222315527824 35.0 32.5 35.0 26.0 36.0 88-89 31.542283617308936 34.5 32.0 35.0 25.0 35.5 90-91 31.252154043818177 34.0 32.0 35.0 24.5 35.0 92-93 30.95148963328099 34.0 31.5 35.0 24.0 35.0 94-95 30.634591167505018 34.0 31.0 35.0 21.5 35.0 96-97 30.24998950311967 34.0 31.0 35.0 19.0 35.0 98-99 29.726864287933626 34.0 31.0 35.0 8.5 35.0 100-101 27.543941284650202 32.0 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1594697098756228 1101 2 0.21679576597393435 1101 3 0.1529210557856331 1101 4 0.14265585943520165 1101 5 0.18235414476666278 1101 6 0.17845766675804953 1101 7 0.18168314692309906 1101 8 0.14399370391639366 1101 9 0.14893045138892802 1101 10-11 0.18763698023643371 1101 12-13 0.19375171361575383 1101 14-15 0.19970746886709634 1101 16-17 0.2569291504287605 1101 18-19 0.2724381163095657 1101 20-21 0.22739867445385897 1101 22-23 0.22912530174266976 1101 24-25 0.342846287133014 1101 26-27 0.3701409993045033 1101 28-29 0.36090038361302135 1101 30-31 0.3092437986434078 1101 32-33 0.28496911427705385 1101 34-35 0.36093517380921014 1101 36-37 0.3499082581267885 1101 38-39 0.35515045548444846 1101 40-41 0.3963624918402715 1101 42-43 0.5413226471886503 1101 44-45 0.4644777083179221 1101 46-47 0.36368494991944544 1101 48-49 0.40538145988631413 1101 50-51 0.3863389861972948 1101 52-53 0.45498168612139267 1101 54-55 0.31385901746021005 1101 56-57 0.41874325604462825 1101 58-59 0.45167542683110184 1101 60-61 0.5531139319363092 1101 62-63 0.5363673952543522 1101 64-65 0.47107527165011476 1101 66-67 0.36240309844603047 1101 68-69 0.39934533087338764 1101 70-71 0.43661823404080735 1101 72-73 0.46535480872309876 1101 74-75 0.4315373541428613 1101 76-77 0.4264152659161624 1101 78-79 0.62053849276694 1101 80-81 0.6510041251243734 1101 82-83 0.5461290813366375 1101 84-85 0.5624664594263535 1101 86-87 0.5360941205670748 1101 88-89 0.5393574166742212 1101 90-91 0.5900087245225691 1101 92-93 0.6357671470926647 1101 94-95 0.7383215925427784 1101 96-97 0.8622277521749133 1101 98-99 0.7663082146529696 1101 100-101 1.1643221410517235 1102 1 -0.0527871046082069 1102 2 -0.042349508091476196 1102 3 -0.030632374502864934 1102 4 -0.012056914668001184 1102 5 -0.0349874005381281 1102 6 0.0027378703739699972 1102 7 0.020207523406014616 1102 8 0.05731305397760167 1102 9 0.05202114913363687 1102 10-11 0.039387166624088366 1102 12-13 0.008470261958159142 1102 14-15 0.009215348783072841 1102 16-17 0.0370888456093752 1102 18-19 0.0206475984037624 1102 20-21 -0.0517119604731775 1102 22-23 -0.02274613107465484 1102 24-25 0.07634389557787813 1102 26-27 0.1499014722757721 1102 28-29 0.10255560045239775 1102 30-31 -0.0031532473641249226 1102 32-33 -0.030762759523391026 1102 34-35 -0.05772629205414148 1102 36-37 -9.287231991876865E-4 1102 38-39 0.0014942034379643587 1102 40-41 0.1621070538544629 1102 42-43 0.10341917458511318 1102 44-45 0.05832609707220371 1102 46-47 -0.04409424745500701 1102 48-49 -0.07853158523461445 1102 50-51 -0.018748137438173273 1102 52-53 0.12689312931598096 1102 54-55 0.06266040630011815 1102 56-57 0.04480002997655674 1102 58-59 0.012965953552544818 1102 60-61 0.030507187221907373 1102 62-63 -0.012115434412528714 1102 64-65 0.003883119829808379 1102 66-67 0.024476768167112084 1102 68-69 0.12124503038626244 1102 70-71 0.033547528890977674 1102 72-73 0.1532516696023407 1102 74-75 0.11499696091527767 1102 76-77 0.009754948603770686 1102 78-79 0.19526183933212593 1102 80-81 0.241591831850954 1102 82-83 0.2257675837370101 1102 84-85 0.188596592013468 1102 86-87 0.2184423279712 1102 88-89 0.21718163044574368 1102 90-91 0.13324121541728218 1102 92-93 0.09354310157377554 1102 94-95 -0.0971237669083358 1102 96-97 -0.11632664783314439 1102 98-99 -0.10829838775193323 1102 100-101 0.042566293768704355 1103 1 0.005968741265078847 1103 2 0.023253190359774578 1103 3 0.03932292119326064 1103 4 0.03790216903835386 1103 5 0.08222476851700833 1103 6 0.11483429788513888 1103 7 0.05670905017655059 1103 8 0.08927234202445788 1103 9 0.06833103921387362 1103 10-11 0.022890888127264475 1103 12-13 0.055082185611922796 1103 14-15 0.025278849521399138 1103 16-17 0.0694231996813528 1103 18-19 0.04075272773587102 1103 20-21 0.11628834121822251 1103 22-23 0.08406708243032313 1103 24-25 0.14373879034252468 1103 26-27 0.17621742028075715 1103 28-29 0.06692288226107479 1103 30-31 0.11669534789132285 1103 32-33 0.0747039271917842 1103 34-35 0.14708662554266283 1103 36-37 0.04795877771649515 1103 38-39 0.12141520944865647 1103 40-41 0.11470451559845429 1103 42-43 0.1894264045761318 1103 44-45 0.22150861362185736 1103 46-47 0.2019655712988282 1103 48-49 0.2750095296163124 1103 50-51 0.2568233412545098 1103 52-53 0.31500470501256217 1103 54-55 0.27984905692027695 1103 56-57 0.18045790518233673 1103 58-59 0.1493566111434106 1103 60-61 0.10245986311859667 1103 62-63 0.2544289171148222 1103 64-65 0.21816461718302094 1103 66-67 0.3033166105164824 1103 68-69 0.2650133921152218 1103 70-71 0.1420613710321561 1103 72-73 0.16046635917827246 1103 74-75 0.23897381260648842 1103 76-77 0.2069571464207982 1103 78-79 0.4023145505064676 1103 80-81 0.28641264354540397 1103 82-83 0.14596550968092714 1103 84-85 0.2668403287843475 1103 86-87 0.39989139128205764 1103 88-89 0.34095872002014005 1103 90-91 0.15151860571920395 1103 92-93 0.2715215089516221 1103 94-95 0.38398811047965253 1103 96-97 0.2780844948929122 1103 98-99 0.1425301792428364 1103 100-101 0.2118744469546776 1104 1 0.012473120542573213 1104 2 -0.007852445174258094 1104 3 0.015014627257080804 1104 4 -0.007080217697485125 1104 5 0.03752302878975655 1104 6 -0.03256056369312432 1104 7 -0.011191894928892054 1104 8 -0.056691338226471544 1104 9 -0.020879421411947874 1104 10-11 -0.011869553007208822 1104 12-13 -0.003913486503321906 1104 14-15 -0.015557540140747506 1104 16-17 -0.029049796392435212 1104 18-19 -0.009619739395269278 1104 20-21 -0.04963028867492625 1104 22-23 -0.008835248147690322 1104 24-25 0.02475239260436979 1104 26-27 0.026523266311876625 1104 28-29 -0.011712041215837132 1104 30-31 0.11021488873861074 1104 32-33 0.12630982016319336 1104 34-35 0.15555837587529453 1104 36-37 0.03325512437925937 1104 38-39 0.0868786938387629 1104 40-41 0.06643678261524855 1104 42-43 0.05123143613885617 1104 44-45 -0.12159490652054927 1104 46-47 -0.11687607415944967 1104 48-49 -0.160851142524983 1104 50-51 0.02681594733078896 1104 52-53 0.01910080139030157 1104 54-55 0.046729560029184825 1104 56-57 -0.06854905438980552 1104 58-59 -0.11999251989292503 1104 60-61 -0.061170487114658556 1104 62-63 -0.0036746764457831205 1104 64-65 -0.06370752809218061 1104 66-67 -0.07212691833189666 1104 68-69 -0.09615337473177021 1104 70-71 -0.17939348885039408 1104 72-73 -0.08976976324161257 1104 74-75 -0.1485860883594583 1104 76-77 -0.2466482321495178 1104 78-79 0.10879223871402388 1104 80-81 0.11470475137258518 1104 82-83 -0.1498362005369387 1104 84-85 -0.060822869227393994 1104 86-87 0.016425384147698452 1104 88-89 0.12249977287508784 1104 90-91 0.11843837572601856 1104 92-93 -0.037204292052834376 1104 94-95 -0.07217210320047585 1104 96-97 -0.037597444181347583 1104 98-99 -0.12545633359611585 1104 100-101 -0.03152075661031617 1105 1 -0.05162985490721894 1105 2 -0.07139997666879339 1105 3 -0.08819774065931796 1105 4 -0.10048384991500114 1105 5 -0.06246711318441811 1105 6 -0.02959036841121332 1105 7 -0.09701344978136461 1105 8 -0.05618541690095924 1105 9 -0.04717965781856748 1105 10-11 -0.07970404698357925 1105 12-13 -0.07068684769952682 1105 14-15 -0.24199441715536807 1105 16-17 -0.13699018044624722 1105 18-19 -0.19583284852361516 1105 20-21 -0.20487722292188693 1105 22-23 -0.11397109360360957 1105 24-25 -0.129385862487851 1105 26-27 -0.2559530000259329 1105 28-29 -0.23159670231365936 1105 30-31 -0.1890664053783837 1105 32-33 -0.19408636552481084 1105 34-35 -0.32659476111592767 1105 36-37 -0.21573338172063217 1105 38-39 -0.22859173394375887 1105 40-41 -0.23889510735430264 1105 42-43 -0.31037897003040626 1105 44-45 -0.3984185017983677 1105 46-47 -0.3475060996601229 1105 48-49 -0.17369668493029877 1105 50-51 -0.4039372337425462 1105 52-53 -0.27881420289701 1105 54-55 -0.032645682933953424 1105 56-57 -0.09921983724726147 1105 58-59 -0.45615875689846774 1105 60-61 -0.5243443668802215 1105 62-63 -0.21474768070705608 1105 64-65 -0.23822667033233813 1105 66-67 -0.24633811351522894 1105 68-69 -0.6907529900827711 1105 70-71 -0.4726034945606017 1105 72-73 -0.4245428565971139 1105 74-75 -0.5961353459385137 1105 76-77 -0.6852245937195818 1105 78-79 -0.47659163374171243 1105 80-81 -0.5284007685638699 1105 82-83 -0.6625578201175841 1105 84-85 -0.5958961673694319 1105 86-87 -0.5830794128048673 1105 88-89 -0.7312767577694217 1105 90-91 -0.8682216693233009 1105 92-93 -0.7025396602314942 1105 94-95 -0.6548107247136663 1105 96-97 -0.6465523446431867 1105 98-99 -0.9212038509546083 1105 100-101 -1.017137571426371 1106 1 -0.14746356425339968 1106 2 -0.20333057700405988 1106 3 -0.19654105542445421 1106 4 -0.20208901455393402 1106 5 -0.15267191028788574 1106 6 -0.12102062554929915 1106 7 -0.015098710351431066 1106 8 -0.059974259027526955 1106 9 -0.2161868335109105 1106 10-11 -0.3020522335078297 1106 12-13 -0.08993135586106149 1106 14-15 -0.2029374505261643 1106 16-17 -0.1286148362235764 1106 18-19 -0.3602921488936133 1106 20-21 -0.33603289497306577 1106 22-23 -0.3826185959212154 1106 24-25 -0.1400859915628061 1106 26-27 -0.3860125946430699 1106 28-29 -0.36287577494292833 1106 30-31 -0.26388202235392555 1106 32-33 -0.2585775244374062 1106 34-35 -0.3480676581102813 1106 36-37 -0.5767465585779306 1106 38-39 -0.41483721837135334 1106 40-41 -0.1596377532267681 1106 42-43 -0.19682086989340775 1106 44-45 -0.2634975738807128 1106 46-47 -0.2931854044602318 1106 48-49 -0.24747091724881187 1106 50-51 -0.15496853441882052 1106 52-53 -0.17348121276085493 1106 54-55 -0.06825820101675362 1106 56-57 -0.21102315381725845 1106 58-59 -0.42040147387155713 1106 60-61 -0.439793606513895 1106 62-63 -0.4731513968077863 1106 64-65 -0.29562947443100995 1106 66-67 -0.226675889269373 1106 68-69 -0.27132399634783155 1106 70-71 -0.15365128510914872 1106 72-73 -0.24856081360312032 1106 74-75 -0.2545242608064697 1106 76-77 -0.2967523954254503 1106 78-79 -0.06665200773092295 1106 80-81 -0.09921971086026105 1106 82-83 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-0.8570668843890132 2316 72-73 -1.3879722191516066 2316 74-75 -0.9233908853703383 2316 76-77 -0.8324098398855462 2316 78-79 -0.9789919766810726 2316 80-81 -0.9758757767807751 2316 82-83 -0.9051246250762546 2316 84-85 -0.9236222406827963 2316 86-87 -0.9961349063460752 2316 88-89 -0.8790864109231578 2316 90-91 -0.8193710277848467 2316 92-93 -0.8386747243853421 2316 94-95 -0.9083641469755754 2316 96-97 -1.097353647564681 2316 98-99 -1.1878973388277032 2316 100-101 -1.7036480549522608 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 11.0 5 49.0 6 70.0 7 160.0 8 390.0 9 721.0 10 1194.0 11 2067.0 12 3015.0 13 4371.0 14 5271.0 15 6829.0 16 9385.0 17 13567.0 18 12784.0 19 9122.0 20 8539.0 21 9374.0 22 9714.0 23 10560.0 24 12463.0 25 15124.0 26 18762.0 27 23421.0 28 29404.0 29 36409.0 30 45989.0 31 57978.0 32 74120.0 33 98508.0 34 142942.0 35 228721.0 36 438367.0 37 858253.0 38 728712.0 39 60703.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.414567277550766 18.40883876920058 13.703163144557983 24.47343080869067 2 16.533425508736677 20.125780459284773 37.69863144383834 25.64216258814021 3 19.328411928131853 23.302798765091637 29.09572286511908 28.27306644165743 4 14.130344234541692 16.403037916857013 35.119917770671535 34.34670007792975 5 15.337535005067066 36.01404602983673 33.608727241457956 15.03969172363825 6 34.39600116490492 34.37564555987487 16.804929146707146 14.423424128513062 7 30.159636556015553 30.83358665804523 20.457395262128095 18.549381523811125 8 27.20060327771937 33.97965113349703 19.661412059508173 19.158333529275428 9 26.824537825626482 15.745520174372848 19.39027949973615 38.03966250026452 10-11 25.992138903225342 25.346839026551482 27.019291312199883 21.641730758023296 12-13 26.588329148577046 22.472863464978207 27.20724200767532 23.731565378769428 14-15 23.663444824198248 23.97573121268359 24.652754801273062 27.7080691618451 16-17 24.048923859828857 27.039560642577026 25.36309968677309 23.548415810821023 18-19 24.087424509451047 26.064820467825157 26.802119823826132 23.045635198897664 20-21 24.455341066105444 25.489932297516066 26.71291348757562 23.341813148802874 22-23 24.47921197820008 25.177531637597305 26.571215034891633 23.772041349310985 24-25 24.400317224227546 25.407463838655005 26.465012290591368 23.727206646526085 26-27 24.10607952084408 25.768335528725654 26.430614545819154 23.694970404611112 28-29 24.08969541355159 25.723996180115034 26.529541126745137 23.656767279588237 30-31 24.218666941662807 25.47514512456536 26.3832706054377 23.922917328334137 32-33 24.065012057371522 25.514936595928074 26.404686301757767 24.015365044942637 34-35 24.513655143039443 25.507431319518776 26.160381064541372 23.81853247290041 36-37 24.29040657195512 25.42391868258587 26.196455614395948 24.08921913106306 38-39 24.232195062636155 25.566424353841327 26.519390092522038 23.681990491000477 40-41 24.47171247453874 25.279049347815572 26.426418306309017 23.82281987133667 42-43 24.370938651350514 25.41683118779863 26.135775238409387 24.076454922441478 44-45 24.267553760646194 25.581231694407386 26.005324377746312 24.145890167200108 46-47 24.329119073103477 25.55549080145626 25.887410610651497 24.227979514788764 48-49 24.46898096409913 25.700750304243215 25.783147058892688 24.047121672764966 50-51 24.19644988024237 25.75932016024564 25.84386681533711 24.20036314417489 52-53 24.573683614745086 25.586423309069005 25.59085719429789 24.249035881888016 54-55 24.31999846829291 25.4137414616147 25.843308043931106 24.422952026161287 56-57 24.27212088110675 25.85130567799121 25.837752101560124 24.03882133934192 58-59 24.379370506049934 25.537846424221495 25.89475918843368 24.18802388129489 60-61 24.43655000686919 25.390645337801278 26.113136412052018 24.059668243277514 62-63 24.415088635657483 25.54524155421009 25.978569569124055 24.061100241008372 64-65 24.300177357375112 25.444703463843283 25.871620804557548 24.38349837422406 66-67 24.165579186211925 25.708877703375638 25.75449309974757 24.371050010664867 68-69 23.967158263733197 25.44132804602384 26.121460881529146 24.470052808713817 70-71 23.954099558892487 25.52189212684226 25.946305271120558 24.577703043144695 72-73 24.139076573739572 25.451342177614528 25.84998983898571 24.559591409660186 74-75 23.958392466116702 25.522873764257785 25.831685453116954 24.68704831650855 76-77 24.093652405173202 25.42756348728491 25.919170594602587 24.559613512939304 78-79 23.829782588614666 25.528077055499104 25.880217999210636 24.761922356675594 80-81 23.803935070497047 25.537079180067686 26.038124669348267 24.620861080086996 82-83 23.831532487032142 25.552787175992524 25.84402966336896 24.77165067360637 84-85 23.75199209828306 25.4030256884737 25.80986804327537 25.035114169967866 86-87 23.82758244249809 25.288899339116416 26.056632768741128 24.826885449644365 88-89 23.597965244976095 25.284323558832757 25.95221268188209 25.165498514309053 90-91 23.51855021167827 25.452601181407786 26.075047996475696 24.953800610438247 92-93 23.557331518478534 25.25256020160475 26.167820098262833 25.022288181653884 94-95 23.449536433806994 25.13915719329573 26.030801177952917 25.38050519494436 96-97 23.58684707970874 25.051581973139587 25.960751513868978 25.400819433282695 98-99 23.22562353896764 24.975721542977823 26.020610570862026 25.77804434719252 100-101 23.04933884731976 25.00180128856374 26.282891151281202 25.6659687128353 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 111.0 1 94.5 2 122.5 3 179.0 4 142.5 5 142.5 6 296.0 7 620.5 8 1080.0 9 1370.0 10 1315.5 11 1245.5 12 1350.5 13 1478.5 14 1563.0 15 1659.0 16 1743.5 17 1813.5 18 1937.0 19 2022.0 20 2180.5 21 2538.5 22 2950.5 23 3397.0 24 3999.0 25 4961.5 26 6311.0 27 8053.0 28 10202.0 29 12736.0 30 15954.0 31 19782.5 32 23726.0 33 27783.5 34 32820.5 35 37693.0 36 42792.0 37 48810.5 38 54671.5 39 58255.0 40 61214.5 41 68437.5 42 76682.5 43 84935.5 44 93172.0 45 99243.0 46 104963.5 47 112869.5 48 124812.5 49 133906.0 50 137978.5 51 140588.5 52 138669.5 53 138565.5 54 139017.5 55 133060.5 56 125976.5 57 116469.0 58 104486.5 59 91447.0 60 75598.5 61 58838.5 62 45778.5 63 35829.5 64 26887.5 65 19802.0 66 14048.0 67 10388.0 68 7332.5 69 4756.0 70 3404.0 71 2488.5 72 1744.0 73 1251.0 74 928.5 75 675.0 76 443.0 77 248.0 78 125.0 79 62.0 80 34.5 81 21.5 82 15.0 83 12.5 84 5.0 85 2.5 86 4.0 87 3.0 88 1.0 89 0.5 90 1.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02462148249540237 2 0.004769782420664579 3 4.0308020456320383E-4 4 0.0011756505966426778 5 2.0154010228160191E-4 6 2.687201363754692E-4 7 0.0 8 1.6795008523466827E-4 9 2.0154010228160191E-4 10-11 3.1910516194586967E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.004349907207577908 20-21 1.847450937581351E-4 22-23 0.0 24-25 3.359001704693365E-5 26-27 2.1833511080506876E-4 28-29 7.389803750325404E-4 30-31 0.002132966082480287 32-33 0.001948220988722152 34-35 0.001561935792682415 36-37 1.6795008523466825E-5 38-39 1.6795008523466825E-5 40-41 1.0077005114080096E-4 42-43 1.6795008523466825E-5 44-45 4.534652301336043E-4 46-47 1.6795008523466825E-5 48-49 5.374402727509384E-4 50-51 6.885953494621399E-4 52-53 6.71800340938673E-5 54-55 1.5115507671120142E-4 56-57 3.359001704693365E-5 58-59 5.71030289797872E-4 60-61 4.702602386570711E-4 62-63 0.0 64-65 0.0011756505966426778 66-67 3.3590017046933654E-4 68-69 3.1910516194586967E-4 70-71 7.725703920794739E-4 72-73 3.3590017046933654E-4 74-75 6.046203068448057E-4 76-77 8.397504261733414E-5 78-79 0.0 80-81 0.0 82-83 8.397504261733414E-5 84-85 1.6795008523466825E-5 86-87 0.0 88-89 3.0231015342240284E-4 90-91 0.0011252655710722772 92-93 0.005407992744556318 94-95 0.0015451407841589478 96-97 5.878252983213389E-4 98-99 0.00524004265932165 100-101 0.001326805673353879 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2977075.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.69823607061307 #Duplication Level Percentage of deduplicated Percentage of total 1 80.47387642115159 44.82252966422645 2 10.399519469977795 11.584697809195204 3 3.3899691597441985 5.664459075945906 4 1.6310286306211743 3.6338167082506776 5 0.9831782618544658 2.738064746413254 6 0.6579316005652942 2.198737776396124 7 0.43910100158028537 1.7120005871402963 8 0.3150843547114261 1.403971421669904 9 0.2285011121183774 1.145439799665031 >10 1.3218826554581968 13.216702757194712 >50 0.09953737137900723 3.8171508304409802 >100 0.05629125664048812 5.863856114861886 >500 0.003134303210116787 1.235253546350939 >1k 9.644009876095267E-4 0.9633191622487479 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4957 0.1665057145016501 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3616 0.12146150164171207 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 3.359001704693365E-5 0.0 4 0.0 0.0 0.0 3.359001704693365E-5 0.0 5 3.359001704693365E-5 0.0 0.0 3.359001704693365E-5 0.0 6 6.71800340938673E-5 0.0 0.0 3.359001704693365E-5 0.0 7 6.71800340938673E-5 0.0 0.0 3.359001704693365E-5 0.0 8 6.71800340938673E-5 0.0 0.0 3.359001704693365E-5 0.0 9 6.71800340938673E-5 0.0 0.0 3.359001704693365E-5 0.0 10-11 1.0077005114080096E-4 0.0 0.0 3.359001704693365E-5 0.0 12-13 1.0077005114080096E-4 0.0 0.0 3.359001704693365E-5 3.359001704693365E-5 14-15 1.0077005114080096E-4 0.0 0.0 5.038502557040048E-5 1.0077005114080096E-4 16-17 1.343600681877346E-4 0.0 0.0 1.0077005114080096E-4 1.343600681877346E-4 18-19 1.343600681877346E-4 0.0 0.0 1.0077005114080096E-4 2.0154010228160191E-4 20-21 1.343600681877346E-4 0.0 0.0 1.0077005114080096E-4 2.0154010228160191E-4 22-23 1.343600681877346E-4 0.0 0.0 1.1756505966426778E-4 2.1833511080506874E-4 24-25 1.343600681877346E-4 0.0 0.0 2.519251278520024E-4 2.3513011932853556E-4 26-27 1.343600681877346E-4 0.0 0.0 5.206452642274716E-4 2.3513011932853556E-4 28-29 1.343600681877346E-4 0.0 0.0 0.0015787308012058816 2.3513011932853556E-4 30-31 1.343600681877346E-4 0.0 0.0 0.006818773460527531 2.3513011932853556E-4 32-33 1.343600681877346E-4 0.0 0.0 0.01703013864279536 2.687201363754692E-4 34-35 1.343600681877346E-4 0.0 0.0 0.03310296179975311 2.687201363754692E-4 36-37 1.343600681877346E-4 0.0 0.0 0.0568511038519352 2.687201363754692E-4 38-39 2.0154010228160191E-4 0.0 0.0 0.0917847215807462 3.0231015342240284E-4 40-41 2.0154010228160191E-4 0.0 0.0 0.13254620726720018 3.0231015342240284E-4 42-43 2.3513011932853556E-4 0.0 0.0 0.17888363578344516 3.0231015342240284E-4 44-45 2.3513011932853556E-4 0.0 0.0 0.2379348857519545 3.0231015342240284E-4 46-47 3.359001704693365E-4 0.0 0.0 0.3059882602890421 3.0231015342240284E-4 48-49 3.359001704693365E-4 0.0 0.0 0.3801214279116247 3.0231015342240284E-4 50-51 3.359001704693365E-4 0.0 0.0 0.45606845645474164 3.0231015342240284E-4 52-53 3.359001704693365E-4 0.0 0.0 0.5418405649840867 3.0231015342240284E-4 54-55 3.359001704693365E-4 3.359001704693365E-5 0.0 0.6426442061419346 3.359001704693365E-4 56-57 3.359001704693365E-4 6.71800340938673E-5 0.0 0.7628124921273398 3.359001704693365E-4 58-59 3.359001704693365E-4 6.71800340938673E-5 0.0 0.8936456085251463 3.359001704693365E-4 60-61 3.359001704693365E-4 6.71800340938673E-5 0.0 1.0329266142102567 3.359001704693365E-4 62-63 3.359001704693365E-4 6.71800340938673E-5 0.0 1.1831915554697146 3.694901875162702E-4 64-65 3.694901875162702E-4 6.71800340938673E-5 0.0 1.3543326923238412 3.694901875162702E-4 66-67 3.694901875162702E-4 6.71800340938673E-5 0.0 1.5586943560373858 3.694901875162702E-4 68-69 3.694901875162702E-4 6.71800340938673E-5 0.0 1.780724368717617 4.0308020456320383E-4 70-71 3.694901875162702E-4 6.71800340938673E-5 0.0 2.021951076140171 4.0308020456320383E-4 72-73 3.694901875162702E-4 6.71800340938673E-5 0.0 2.276445840296264 4.534652301336043E-4 74-75 3.694901875162702E-4 6.71800340938673E-5 0.0 2.563875616166875 4.702602386570711E-4 76-77 3.694901875162702E-4 6.71800340938673E-5 0.0 2.893998303704139 4.702602386570711E-4 78-79 3.694901875162702E-4 6.71800340938673E-5 0.0 3.2602134645583334 4.702602386570711E-4 80-81 4.3667022161013747E-4 6.71800340938673E-5 0.0 3.642518243578009 4.702602386570711E-4 82-83 4.702602386570711E-4 6.71800340938673E-5 0.0 4.041584441104104 5.374402727509384E-4 84-85 4.8705524718053794E-4 6.71800340938673E-5 0.0 4.471469479270761 5.374402727509384E-4 86-87 5.038502557040048E-4 6.71800340938673E-5 0.0 4.946314755254738 5.71030289797872E-4 88-89 5.038502557040048E-4 6.71800340938673E-5 0.0 5.467581434797578 5.71030289797872E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2840 0.0 45.67276 1 GTATCAA 5380 0.0 45.3052 1 TCAACGC 6375 0.0 37.180614 4 ATCAACG 6435 0.0 36.833942 3 CAACGCA 6520 0.0 36.352524 5 TATCAAC 6780 0.0 35.451855 2 AACGCAG 6825 0.0 34.72798 6 ACGCAGA 7890 0.0 30.100563 7 CGCAGAG 8330 0.0 28.453598 8 GTACATG 7470 0.0 24.869617 1 GCAGAGT 9935 0.0 23.617868 9 TACATGG 7915 0.0 22.506006 2 GTGGTAT 1555 0.0 22.305164 1 ACATGGG 7940 0.0 21.77595 3 CATGGGG 6585 0.0 19.836868 4 AGAGTAC 8850 0.0 19.83104 10-11 CAGAGTA 9085 0.0 19.579483 10-11 TGGTATC 1740 0.0 19.110273 2 GAGTACT 5520 0.0 18.673748 12-13 GTCCTAC 1125 0.0 18.160543 1 >>END_MODULE