FastQCFastQC Report
Fri 27 May 2016
SRR940220_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940220_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991137
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44040.14723498121282977No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31010.10367295112193123No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26750.048.6548731
GTATCAA48050.042.9038161
ATCAACG56800.036.0448343
TCAACGC57300.035.813214
CAACGCA59150.034.5324865
TATCAAC62200.033.1440962
AACGCAG62600.032.626066
ACGCAGA72950.027.8022797
CGCAGAG77550.026.091898
GTACATG75500.024.4109331
ACATGGG78450.022.8882683
TACATGG80900.022.6644742
GCAGAGT91350.021.7917569
TATAAGC17750.020.8742263
CCTATAA16450.020.5018021
AGAGTAC81400.019.69945710-11
CATGGGG68200.019.641694
GTGGTAT13350.019.2137471
CAGAGTA84350.019.12315810-11
TTATAGG15200.019.0633983
Produced by FastQC (version 0.11.5)