FastQCFastQC Report
Fri 27 May 2016
SRR940220_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940220_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991137
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50110.16752826767881243No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37770.12627305268865988No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30000.052.442921
GTATCAA57050.041.9908871
TCAACGC65100.035.8970874
ATCAACG65000.035.8792423
CAACGCA66950.034.904585
TATCAAC68350.034.7467232
AACGCAG70100.033.20066
ACGCAGA79800.029.164947
CGCAGAG85100.027.404378
GTACATG75000.024.1459241
GCAGAGT99700.023.2007319
TACATGG79650.022.4821572
ACATGGG80100.021.4660023
GAGTACT57900.019.85367812-13
CATGGGG66500.019.6420214
CAGAGTA95150.019.59290110-11
GTACTTT60000.019.3567214-15
GTGGTAT14350.019.2112771
AGAGTAC92950.019.08574510-11
TATAACG1759.945521E-518.9995082
Produced by FastQC (version 0.11.5)