##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940220_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2991137 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.789359029693394 34.0 31.0 34.0 31.0 34.0 2 32.863681603350166 34.0 31.0 34.0 31.0 34.0 3 32.92550558533427 34.0 31.0 34.0 31.0 34.0 4 36.33390914558578 37.0 37.0 37.0 35.0 37.0 5 36.30504453657589 37.0 37.0 37.0 35.0 37.0 6 36.352354639723956 37.0 37.0 37.0 35.0 37.0 7 36.21463476932016 37.0 37.0 37.0 35.0 37.0 8 36.238067330249336 37.0 37.0 37.0 35.0 37.0 9 38.07499823645657 39.0 39.0 39.0 35.0 39.0 10-11 38.02814364571064 39.0 38.0 39.0 35.0 39.0 12-13 37.997428235483696 39.0 38.0 39.0 35.0 39.0 14-15 39.43519671616512 41.0 39.0 41.0 36.0 41.0 16-17 39.37554498506755 41.0 39.0 41.0 36.0 41.0 18-19 39.33178871445875 41.0 39.0 41.0 36.0 41.0 20-21 39.266248754236265 40.0 39.0 41.0 36.0 41.0 22-23 39.18590990650044 40.0 39.0 41.0 36.0 41.0 24-25 39.065271166115096 40.0 38.5 41.0 36.0 41.0 26-27 38.877282284295234 40.0 38.0 41.0 35.0 41.0 28-29 38.724225603842285 40.0 38.0 41.0 35.0 41.0 30-31 38.7985889646646 40.0 38.0 41.0 35.0 41.0 32-33 38.70797208553135 40.0 38.0 41.0 35.0 41.0 34-35 38.69199973120589 40.0 38.0 41.0 35.0 41.0 36-37 38.78924803511173 40.0 38.0 41.0 35.0 41.0 38-39 38.69925282593208 40.0 38.0 41.0 35.0 41.0 40-41 38.533068528790224 40.0 38.0 41.0 34.5 41.0 42-43 38.36174337718399 40.0 38.0 41.0 34.0 41.0 44-45 38.20750804794297 40.0 38.0 41.0 34.0 41.0 46-47 37.98443300992231 40.0 38.0 41.0 33.0 41.0 48-49 37.76113915878811 40.0 37.5 41.0 33.0 41.0 50-51 37.516846436656024 40.0 37.0 41.0 33.0 41.0 52-53 37.26628151769712 40.0 36.5 41.0 32.0 41.0 54-55 37.0594130258828 39.0 36.0 41.0 32.0 41.0 56-57 36.83342003392021 39.0 36.0 41.0 31.5 41.0 58-59 36.51899077173664 39.0 35.0 40.0 31.0 41.0 60-61 36.18511422245119 38.0 35.0 40.0 31.0 41.0 62-63 35.79791647791458 38.0 35.0 40.0 30.0 41.0 64-65 35.420264434561176 37.0 34.5 40.0 30.0 41.0 66-67 34.992786355155246 36.5 34.0 39.0 29.0 41.0 68-69 34.541856992842526 36.0 34.0 39.0 29.0 40.0 70-71 34.10757899086535 35.0 34.0 38.5 28.0 40.0 72-73 33.679825598091966 35.0 33.5 37.0 28.0 39.5 74-75 33.23194524356457 35.0 33.0 37.0 27.0 39.0 76-77 31.926159684427695 34.0 31.5 35.5 25.5 37.5 78-79 32.64576129411658 35.0 33.0 36.0 27.0 37.5 80-81 32.52845840895954 35.0 33.0 35.5 27.0 37.0 82-83 32.25226928756523 35.0 33.0 35.0 26.0 37.0 84-85 31.983545889071614 35.0 33.0 35.0 26.0 36.0 86-87 31.680573139913015 35.0 32.0 35.0 25.5 36.0 88-89 31.372613156802913 34.0 32.0 35.0 25.0 35.5 90-91 31.06848399120468 34.0 31.5 35.0 24.0 35.0 92-93 30.75628682337185 34.0 31.0 35.0 23.0 35.0 94-95 30.416211962207015 34.0 31.0 35.0 20.0 35.0 96-97 30.00621435928879 34.0 31.0 35.0 17.5 35.0 98-99 29.484755462554876 34.0 30.5 35.0 2.0 35.0 100-101 27.210371340396645 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.0020633455368681552 1101 2 0.03394450465474108 1101 3 0.051353617947903274 1101 4 0.03871535444929464 1101 5 0.06023369621015462 1101 6 0.05892372888148145 1101 7 0.21337563286972738 1101 8 0.10767174842225558 1101 9 0.11167084440447184 1101 10-11 0.1176146630599888 1101 12-13 0.10588965412500073 1101 14-15 0.13416326501695863 1101 16-17 0.18529859452979025 1101 18-19 0.18158330468911288 1101 20-21 0.17763947055706097 1101 22-23 0.09533369729685859 1101 24-25 0.2125403192801869 1101 26-27 0.10866125644031399 1101 28-29 0.1226572843393754 1101 30-31 0.16425824785869736 1101 32-33 0.22985414757908273 1101 34-35 0.19735571140613217 1101 36-37 0.18356653093453446 1101 38-39 0.26834208449162844 1101 40-41 0.27471373779187047 1101 42-43 0.33887701951434224 1101 44-45 0.2139411398758213 1101 46-47 0.14994449315918246 1101 48-49 0.17571770413330512 1101 50-51 0.2531152248162414 1101 52-53 0.2645896749295602 1101 54-55 0.33735459936299605 1101 56-57 0.2419645252275231 1101 58-59 0.2074700335811812 1101 60-61 0.2581108045880285 1101 62-63 0.3537702520865835 1101 64-65 0.31188264279333566 1101 66-67 0.20389282354225458 1101 68-69 0.2678062644572421 1101 70-71 0.33547211036564306 1101 72-73 0.05531316513465612 1101 74-75 0.1180607073686204 1101 76-77 0.08992620086413083 1101 78-79 0.38501120981843684 1101 80-81 0.3573471718377377 1101 82-83 0.38692111911903737 1101 84-85 0.2676819094429703 1101 86-87 0.2157577845792531 1101 88-89 0.31433237586825413 1101 90-91 0.33935030882012995 1101 92-93 0.3667937380024249 1101 94-95 0.3758163054782102 1101 96-97 0.31735213458514977 1101 98-99 0.40279176024796115 1101 100-101 0.6901106250367022 1102 1 0.04594319560603566 1102 2 0.0557311227632411 1102 3 0.07234029619941396 1102 4 0.08551891728558303 1102 5 0.03403513480368048 1102 6 0.05360905026834928 1102 7 0.20415956566817073 1102 8 0.12091277119812105 1102 9 0.07829513007340694 1102 10-11 0.08383785761000695 1102 12-13 0.08664819727142259 1102 14-15 0.15386924876300867 1102 16-17 0.18141325666731944 1102 18-19 0.18995889214863126 1102 20-21 0.1341601782949411 1102 22-23 0.02870182293910517 1102 24-25 0.1950450869949023 1102 26-27 0.23970605130200795 1102 28-29 0.1552512929102292 1102 30-31 0.20940610750029265 1102 32-33 0.1791601720184346 1102 34-35 0.19065860500976584 1102 36-37 0.22821711312150939 1102 38-39 0.2221438464600709 1102 40-41 0.16634950386056602 1102 42-43 0.16740009190181837 1102 44-45 0.24314856328937395 1102 46-47 0.2352193447283213 1102 48-49 0.2975048297279912 1102 50-51 0.3385246192353293 1102 52-53 0.33011570147674973 1102 54-55 0.3854064602027307 1102 56-57 0.45156381531334944 1102 58-59 0.4705719992404127 1102 60-61 0.38397434249612417 1102 62-63 0.24307647952041833 1102 64-65 0.4168749850627904 1102 66-67 0.40204328422759517 1102 68-69 0.33957059448047033 1102 70-71 0.3563761745864795 1102 72-73 0.3387295078657999 1102 74-75 0.2756855044183979 1102 76-77 0.1900572975287993 1102 78-79 0.4163172066411036 1102 80-81 0.37614068361501296 1102 82-83 0.3730141682459518 1102 84-85 0.33824420531993127 1102 86-87 0.2830966580094838 1102 88-89 0.3769752317550399 1102 90-91 0.34999099597059313 1102 92-93 0.316380945196169 1102 94-95 0.48079336415199236 1102 96-97 0.45544414230318964 1102 98-99 0.41592668300443236 1102 100-101 0.7134909268068732 1103 1 0.0039345116230578014 1103 2 0.02809094728245043 1103 3 0.030918249246056462 1103 4 0.03922169840231504 1103 5 0.024265855995452057 1103 6 0.0022579320153255367 1103 7 0.10257587660218803 1103 8 0.08630973026849631 1103 9 -0.01886043657628278 1103 10-11 0.04549966719300613 1103 12-13 -0.022686870841859275 1103 14-15 0.018710605646028 1103 16-17 0.03987942424415536 1103 18-19 -0.007813815270736768 1103 20-21 -0.004908647125184018 1103 22-23 0.009573426323989054 1103 24-25 0.05582657157106041 1103 26-27 -0.03738919599130952 1103 28-29 0.048967349273105754 1103 30-31 0.015390948226780665 1103 32-33 0.02143799781990907 1103 34-35 0.02616649401355886 1103 36-37 -0.033199939161924874 1103 38-39 -0.018133894595962374 1103 40-41 0.12701584316814518 1103 42-43 0.13446742515564125 1103 44-45 0.09286037683832404 1103 46-47 0.1975222870461124 1103 48-49 0.18524113701818123 1103 50-51 0.040193885031555965 1103 52-53 0.2540534364389657 1103 54-55 0.06222227845863415 1103 56-57 -0.007073309447058307 1103 58-59 -0.014956966439179098 1103 60-61 0.03603525576332345 1103 62-63 0.055033910985422096 1103 64-65 -0.018982646292933225 1103 66-67 -0.08693980859532502 1103 68-69 0.12564343191596805 1103 70-71 0.1006568676471602 1103 72-73 0.07643319277995175 1103 74-75 0.12046384025118329 1103 76-77 0.1166740967766664 1103 78-79 0.21295083836535866 1103 80-81 0.30481787080071854 1103 82-83 0.275840590303865 1103 84-85 0.19337199139842198 1103 86-87 0.05589892530791474 1103 88-89 0.16700864847527086 1103 90-91 0.16145432919346803 1103 92-93 0.1460944513447764 1103 94-95 0.05273814383867048 1103 96-97 0.20589832128671404 1103 98-99 0.096806414562284 1103 100-101 0.5096470860973596 1104 1 -0.06246628421061473 1104 2 -0.005282886263024977 1104 3 -0.001867895898570282 1104 4 0.028707254890314005 1104 5 -0.050953868538556435 1104 6 -0.06856583824669116 1104 7 0.09050101906033348 1104 8 0.02572626151539481 1104 9 -0.05685786921109326 1104 10-11 -0.09124917527475418 1104 12-13 -0.16556163309262928 1104 14-15 -0.04905622796767517 1104 16-17 -0.06132958139382794 1104 18-19 0.021030032768805995 1104 20-21 0.041003777395694385 1104 22-23 0.03233678221840108 1104 24-25 0.06387503177115406 1104 26-27 0.07520385185756595 1104 28-29 -0.008377134528210206 1104 30-31 0.08416968669620672 1104 32-33 0.05440720452388348 1104 34-35 0.10620402501074722 1104 36-37 0.15515374297610407 1104 38-39 0.10013456822058941 1104 40-41 0.14462910096813175 1104 42-43 0.16037277489891721 1104 44-45 0.1037803478637116 1104 46-47 -0.08831218207198077 1104 48-49 -0.06825009235164003 1104 50-51 0.0600512231093262 1104 52-53 -0.11923159986599075 1104 54-55 -0.13277053755729185 1104 56-57 -0.05371449010180385 1104 58-59 0.02371816549236172 1104 60-61 0.03884591494480816 1104 62-63 0.07446297958487236 1104 64-65 -0.00544307110833131 1104 66-67 0.07867781926461248 1104 68-69 0.0287605784793854 1104 70-71 0.16676685904798916 1104 72-73 0.11790406651498131 1104 74-75 0.05567603516828967 1104 76-77 -0.01658346002927047 1104 78-79 0.09823693341011364 1104 80-81 0.005676819054635018 1104 82-83 0.004119424357099888 1104 84-85 0.05604525533649252 1104 86-87 0.002743353830481965 1104 88-89 0.05123020565138248 1104 90-91 0.05074925847834422 1104 92-93 0.02795309584710992 1104 94-95 0.0014923907880408649 1104 96-97 -0.01458592945223458 1104 98-99 0.03576090113540431 1104 100-101 0.5993741014576415 1105 1 -0.09066362659959282 1105 2 -0.07337836145190835 1105 3 -0.06827639555538667 1105 4 -0.029159643660612744 1105 5 -0.02344157233212485 1105 6 -0.09568608467047568 1105 7 0.11153759079645909 1105 8 0.004962086581862479 1105 9 0.014670068664024427 1105 10-11 0.04489071439906667 1105 12-13 -0.051860293066916086 1105 14-15 -0.05915561883320919 1105 16-17 -0.0034207166420188173 1105 18-19 -0.007115043620636641 1105 20-21 0.009453740973860647 1105 22-23 -0.0027774228256092215 1105 24-25 -0.001728768723054941 1105 26-27 0.06294446172836388 1105 28-29 -0.01344386845767076 1105 30-31 -0.17630137349932085 1105 32-33 -0.03675565589237095 1105 34-35 -0.1651036286600629 1105 36-37 -0.018449363267848184 1105 38-39 -0.09573374425163905 1105 40-41 -0.061463843538305696 1105 42-43 -0.07538304185759159 1105 44-45 -0.03802037689148818 1105 46-47 0.013195839937154119 1105 48-49 -0.04128554820319863 1105 50-51 -0.11338588106065117 1105 52-53 -0.020562925542115806 1105 54-55 0.015144870853823988 1105 56-57 0.023404923572293512 1105 58-59 0.13703939911896867 1105 60-61 0.02474351829123833 1105 62-63 0.03242303652742606 1105 64-65 0.10971897759612403 1105 66-67 0.028428169676914194 1105 68-69 0.03530796325092922 1105 70-71 0.06537027209446222 1105 72-73 -0.005035535465729879 1105 74-75 0.21479532349164998 1105 76-77 0.07693947871804241 1105 78-79 0.01914740931340475 1105 80-81 0.1989502397010412 1105 82-83 0.2629589526175593 1105 84-85 0.11377595548612618 1105 86-87 0.03290664289367129 1105 88-89 0.07388055860576159 1105 90-91 0.17784921210733629 1105 92-93 0.10575295366605886 1105 94-95 0.1537785297134775 1105 96-97 0.31277048916314243 1105 98-99 0.42720387224600387 1105 100-101 0.3644249561656139 1106 1 -0.15015961277985923 1106 2 -0.09528206512288762 1106 3 -0.10105474522823243 1106 4 -0.01077427278543297 1106 5 -0.2020139160759129 1106 6 -0.09028388535350729 1106 7 0.03009084846292609 1106 8 -0.009845307505869982 1106 9 -0.09441349035922997 1106 10-11 -0.09350808741941563 1106 12-13 -0.09998360591511357 1106 14-15 -0.14217751480524754 1106 16-17 -0.051137345828699665 1106 18-19 0.005630104230185395 1106 20-21 -0.2649254092538982 1106 22-23 -0.13080656443812444 1106 24-25 -0.12786984664462153 1106 26-27 -0.05227072053926207 1106 28-29 0.08367278635383713 1106 30-31 0.0021570944011628512 1106 32-33 0.03933284695508377 1106 34-35 -0.013947197933241284 1106 36-37 -0.0646005585776166 1106 38-39 0.04691292474188202 1106 40-41 -0.021515512907363643 1106 42-43 0.10819520001426497 1106 44-45 0.03433319710903504 1106 46-47 0.20751220934110393 1106 48-49 0.17982522173446114 1106 50-51 0.2951210089249088 1106 52-53 0.07246811584197843 1106 54-55 0.053629162393662 1106 56-57 -0.01937196813630493 1106 58-59 0.04055331384130767 1106 60-61 0.1130420298422834 1106 62-63 0.04066285397262703 1106 64-65 -0.0030077247980244692 1106 66-67 -0.059001527729236614 1106 68-69 0.03440292090369468 1106 70-71 0.17371126342178655 1106 72-73 0.12464718058910051 1106 74-75 0.21237653872693585 1106 76-77 0.08753744286979881 1106 78-79 0.19935002216204367 1106 80-81 0.29787543288130536 1106 82-83 0.30838635704358097 1106 84-85 0.2968621344269309 1106 86-87 0.25723388075832787 1106 88-89 0.45056019968147254 1106 90-91 0.4421107532197155 1106 92-93 0.30272922419353065 1106 94-95 0.2927743237075511 1106 96-97 0.43857590395396784 1106 98-99 0.3555663575006811 1106 100-101 0.5623990485828756 1107 1 -0.062341797704029034 1107 2 -0.044234058981771796 1107 3 0.05410559824970562 1107 4 0.019976443695853163 1107 5 -0.02045295588812479 1107 6 -0.05013657225308066 1107 7 0.05293466474488895 1107 8 -0.015307455531392122 1107 9 -0.01031033437688933 1107 10-11 -0.07978293689608762 1107 12-13 -0.04116690711009596 1107 14-15 0.054084602038315666 1107 16-17 -0.0025471926148483703 1107 18-19 -0.008585350111523837 1107 20-21 0.04722369897456957 1107 22-23 0.0027748350904701624 1107 24-25 0.08183916504970057 1107 26-27 0.01483740181961224 1107 28-29 0.06821595441918049 1107 30-31 -0.06007435368682934 1107 32-33 -0.08597770045070519 1107 34-35 0.024555591661076903 1107 36-37 0.037240746907045263 1107 38-39 0.10641968678780955 1107 40-41 0.16523892158941322 1107 42-43 0.14057566105251595 1107 44-45 0.12365629958399182 1107 46-47 0.12426173917563688 1107 48-49 0.1285923433906575 1107 50-51 0.20817899545819785 1107 52-53 0.2076924148163286 1107 54-55 0.15990599322395838 1107 56-57 0.1290265459567479 1107 58-59 0.22222790411795046 1107 60-61 0.13002997173557418 1107 62-63 0.19989027763907785 1107 64-65 0.26076192497876605 1107 66-67 0.1928341476795694 1107 68-69 0.15599572350132718 1107 70-71 0.22106864656252156 1107 72-73 0.2949820943136032 1107 74-75 0.27593895840361427 1107 76-77 0.16638281175344716 1107 78-79 0.39935770938101456 1107 80-81 0.4890716724237123 1107 82-83 0.4372105048720485 1107 84-85 0.3454972741123683 1107 86-87 0.2559131948851636 1107 88-89 0.27545661169908797 1107 90-91 0.34260051568465144 1107 92-93 0.38723135766747063 1107 94-95 0.47780655047834486 1107 96-97 0.4271692078448943 1107 98-99 0.5314529591111032 1107 100-101 0.8672959694727282 1108 1 0.13460734440089084 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-1.2203319680504237 2316 88-89 -1.3236085494011718 2316 90-91 -1.8427501364696255 2316 92-93 -1.3671223465496851 2316 94-95 -1.556311736459886 2316 96-97 -1.4408948592949535 2316 98-99 -1.396040018338553 2316 100-101 -1.5587218189330088 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 3.0 4 20.0 5 39.0 6 67.0 7 159.0 8 429.0 9 824.0 10 1252.0 11 2101.0 12 3075.0 13 4514.0 14 5422.0 15 6827.0 16 9357.0 17 14001.0 18 13270.0 19 9568.0 20 8960.0 21 9822.0 22 10236.0 23 10973.0 24 13111.0 25 16274.0 26 19395.0 27 24563.0 28 30354.0 29 38244.0 30 47699.0 31 59945.0 32 77174.0 33 103187.0 34 149645.0 35 238266.0 36 452516.0 37 856686.0 38 697553.0 39 55603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.373114747956826 18.425354405140244 13.711592939091588 24.489937907811345 2 16.58616579713246 20.171310218935396 37.65117107117364 25.5913529127585 3 19.305726802731275 23.353497409672872 29.113364590214797 28.227411197381052 4 14.113569049831181 16.426446777233235 35.1744972299294 34.28548694300619 5 15.31220402141393 36.02312431694035 33.61775137681758 15.046920284828143 6 34.34345663162963 34.38551445612346 16.83265800101634 14.438370911230574 7 30.278954123465425 30.788693396524465 20.445268805808627 18.487083674201482 8 27.189469151519692 33.96284889754261 19.645813496945642 19.201868453992063 9 26.845477154673958 15.756683829593898 19.357856226578722 38.03998278915342 10-11 25.967471267768698 25.34170012833031 27.055244884883017 21.635583719017976 12-13 26.593766851869372 22.44440826347974 27.230782140704353 23.73104274394653 14-15 23.647663079290584 24.013644309839368 24.669866341795778 27.66882626907427 16-17 24.058628708194917 27.09876890946009 25.33214470725681 23.51045767508818 18-19 24.078765532356567 26.056061499380906 26.791270436563426 23.073902531699105 20-21 24.462754021279213 25.506989429933185 26.715211989750888 23.315044559036714 22-23 24.462637451912098 25.202774062171006 26.607056112775844 23.72753237314105 24-25 24.416638569746695 25.381506582201503 26.49850750609844 23.70334734195336 26-27 24.096980071076818 25.749499353087295 26.450070709063127 23.703449866772758 28-29 24.067259485706415 25.756603185683545 26.545652281477384 23.63048504713266 30-31 24.22340375916862 25.449669495992044 26.410816573827773 23.916110171011564 32-33 24.080597746448547 25.503585469029634 26.408854130970504 24.006962653551316 34-35 24.483668956382182 25.544806918191583 26.190098486240228 23.78142563918601 36-37 24.297543787388676 25.421216299728915 26.217520839535702 24.063719073346707 38-39 24.20329472399177 25.58617345268011 26.524903501720342 23.685628321607773 40-41 24.486407677080653 25.297520641816142 26.425720386595465 23.79035129450774 42-43 24.339955702285927 25.422575118057587 26.17189184671319 24.065577332943292 44-45 24.325198076851713 25.577547935784956 25.9855198875879 24.111734099775436 46-47 24.338069436471816 25.578049417328597 25.885139998602536 24.19874114759705 48-49 24.427015032032394 25.739519994650824 25.777231715624204 24.05623325769258 50-51 24.158248018514758 25.7911272588681 25.840189025710202 24.210435696906934 52-53 24.58242850664188 25.641090362202036 25.59408478622409 24.182396344932 54-55 24.34285165996935 25.434614975070414 25.828095754623387 24.394437610336848 56-57 24.277845561377756 25.879427435023445 25.850575525389765 23.992151478209035 58-59 24.40859945438498 25.54192075209286 25.833383481772714 24.216096311749443 60-61 24.412715861293794 25.408336534497856 26.11222497149041 24.06672263271794 62-63 24.444851201968483 25.523154254154363 25.969506558547174 24.062487985329984 64-65 24.268388963805048 25.470061981768378 25.8608852900158 24.400663764410773 66-67 24.17352764330154 25.741579733288223 25.740493187945766 24.344399435464474 68-69 24.0223366565958 25.481698096743816 26.11105743401255 24.384907812647832 70-71 23.9506841483688 25.5391152667347 25.940184763103545 24.570015821792953 72-73 24.152440588974244 25.523175586504443 25.80547668817747 24.51890713634384 74-75 23.970669971992216 25.5369997818552 25.82038742850743 24.671942817645157 76-77 24.129520519791136 25.483476197664125 25.860642042817005 24.526361239727734 78-79 23.811126967302055 25.57869199853366 25.904138599564945 24.706042434599343 80-81 23.776083735410225 25.55769781113263 26.023657901212115 24.642560552245033 82-83 23.810326975996084 25.53719538757335 25.85692330374704 24.795554332683526 84-85 23.77755117392867 25.403732834061906 25.812388729616103 25.006327262393324 86-87 23.84584331080827 25.289670649298547 26.077220432197052 24.78726560769613 88-89 23.592157851300872 25.318951079262487 25.935331008542526 25.15356006089412 90-91 23.526666185206878 25.43891412094805 26.066634034392276 24.967785659452794 92-93 23.57792966976895 25.266259140785017 26.110908720968045 25.044902468477993 94-95 23.44413419401083 25.151848865245668 26.01205831103408 25.39195862970942 96-97 23.610065649684138 25.071014760940237 25.940175590714265 25.378743998661363 98-99 23.222690426980897 25.0165825614307 25.96625181288184 25.79447519870656 100-101 23.091589654916483 24.971615812499877 26.272123117165513 25.664671415418127 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 86.0 1 79.0 2 139.0 3 195.5 4 147.0 5 142.0 6 276.0 7 626.0 8 1102.5 9 1342.5 10 1238.5 11 1180.0 12 1318.5 13 1471.5 14 1582.5 15 1700.5 16 1770.5 17 1837.0 18 1943.5 19 2049.5 20 2224.0 21 2521.0 22 2967.5 23 3458.0 24 4080.5 25 5046.5 26 6440.0 27 8308.0 28 10372.0 29 12840.5 30 16204.0 31 20268.5 32 24103.5 33 28122.5 34 33142.5 35 37885.5 36 43116.0 37 49342.0 38 55175.5 39 58621.5 40 61705.5 41 68932.5 42 77195.0 43 85645.5 44 93773.5 45 99169.0 46 104997.5 47 113216.5 48 124881.5 49 134205.0 50 138594.5 51 140855.5 52 138933.0 53 138607.0 54 138786.5 55 133517.5 56 126733.5 57 116737.5 58 105050.5 59 92310.0 60 76012.0 61 59580.0 62 46378.5 63 35987.5 64 27097.0 65 19864.0 66 14048.0 67 10450.0 68 7327.5 69 4736.5 70 3309.0 71 2357.5 72 1722.5 73 1255.5 74 945.5 75 710.5 76 471.0 77 264.5 78 143.5 79 78.0 80 46.0 81 32.0 82 16.5 83 7.5 84 4.5 85 4.0 86 4.5 87 3.0 88 2.0 89 2.5 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05181975950951093 2 0.002407111409474056 3 3.00888926184257E-4 4 0.0019390619687429896 5 0.0 6 5.683457494591521E-4 7 0.0 8 3.3432102909361894E-5 9 0.0 10-11 9.19382830007452E-4 12-13 0.0 14-15 0.0 16-17 2.8417287472957606E-4 18-19 0.002758148490022356 20-21 0.0016381730425587327 22-23 0.0 24-25 8.358025727340472E-5 26-27 2.3402472036553322E-4 28-29 1.6716051454680945E-4 30-31 7.856544183700044E-4 32-33 0.001203555704737028 34-35 2.8417287472957606E-4 36-37 2.507407718202142E-4 38-39 7.187902125512807E-4 40-41 0.0 42-43 4.0118523491234265E-4 44-45 0.0 46-47 0.0 48-49 8.190865212793664E-4 50-51 3.17604977638938E-4 52-53 8.358025727340472E-5 54-55 4.1790128636702366E-4 56-57 1.6716051454680947E-5 58-59 5.683457494591521E-4 60-61 0.001270419910555752 62-63 6.686420581872379E-5 64-65 5.181975950951093E-4 66-67 1.8387656600149039E-4 68-69 0.0 70-71 3.17604977638938E-4 72-73 1.504444630921285E-4 74-75 1.504444630921285E-4 76-77 4.1790128636702366E-4 78-79 5.516296980044712E-4 80-81 3.3432102909361894E-5 82-83 0.0 84-85 0.0037443955258485316 86-87 8.190865212793664E-4 88-89 0.0014542964765572423 90-91 0.00778967997788132 92-93 0.016331582271223284 94-95 1.6716051454680945E-4 96-97 0.0011199754474636233 98-99 0.0014375804251025614 100-101 0.0011701236018276664 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2991137.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.453857773292846 #Duplication Level Percentage of deduplicated Percentage of total 1 80.34214330251359 44.55281787899101 2 10.446674323439224 11.58616784271818 3 3.4177301417289594 5.68578963560801 4 1.6664447045644266 3.696431505358909 5 0.980441194926753 2.718462328927272 6 0.6510205604836491 2.16609609411298 7 0.442843774667183 1.7190176987327475 8 0.3179234763422119 1.4104066591897482 9 0.2417476051804692 1.2065253583240703 >10 1.3325100258629496 13.42606154354295 >50 0.09935204189670489 3.7782673665944073 >100 0.057003090702628885 5.849844802607473 >500 0.0031997848934899923 1.25329848816631 >1k 9.055994980365935E-4 0.7830479053512448 >5k 6.037329986910623E-5 0.16776489177455586 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5011 0.16752826767881243 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3777 0.12627305268865988 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.3432102909361894E-5 2 0.0 0.0 0.0 0.0 3.3432102909361894E-5 3 3.3432102909361894E-5 0.0 0.0 0.0 3.3432102909361894E-5 4 3.3432102909361894E-5 0.0 0.0 0.0 6.686420581872379E-5 5 3.3432102909361894E-5 0.0 0.0 0.0 6.686420581872379E-5 6 3.3432102909361894E-5 0.0 0.0 0.0 6.686420581872379E-5 7 1.0029630872808567E-4 3.3432102909361894E-5 0.0 0.0 6.686420581872379E-5 8 1.0029630872808567E-4 3.3432102909361894E-5 0.0 0.0 6.686420581872379E-5 9 1.0029630872808567E-4 3.3432102909361894E-5 0.0 0.0 1.0029630872808567E-4 10-11 1.0029630872808567E-4 5.014815436404284E-5 0.0 3.3432102909361894E-5 1.0029630872808567E-4 12-13 1.3372841163744758E-4 6.686420581872379E-5 0.0 3.3432102909361894E-5 1.1701236018276663E-4 14-15 1.3372841163744758E-4 6.686420581872379E-5 0.0 3.3432102909361894E-5 1.3372841163744758E-4 16-17 1.3372841163744758E-4 6.686420581872379E-5 0.0 3.3432102909361894E-5 1.3372841163744758E-4 18-19 1.3372841163744758E-4 6.686420581872379E-5 0.0 5.014815436404284E-5 2.0059261745617135E-4 20-21 1.3372841163744758E-4 6.686420581872379E-5 0.0 6.686420581872379E-5 2.1730866891085231E-4 22-23 1.6716051454680945E-4 6.686420581872379E-5 0.0 6.686420581872379E-5 2.3402472036553325E-4 24-25 2.0059261745617135E-4 6.686420581872379E-5 0.0 1.0029630872808567E-4 2.3402472036553325E-4 26-27 2.0059261745617135E-4 6.686420581872379E-5 0.0 3.677531320029808E-4 2.3402472036553325E-4 28-29 2.507407718202142E-4 6.686420581872379E-5 0.0 0.001621456991104052 2.3402472036553325E-4 30-31 2.6745682327489515E-4 6.686420581872379E-5 0.0 0.00636881560423344 2.3402472036553325E-4 32-33 2.6745682327489515E-4 6.686420581872379E-5 0.0 0.01738469351286818 2.3402472036553325E-4 34-35 2.6745682327489515E-4 6.686420581872379E-5 0.0 0.031158719911525284 2.6745682327489515E-4 36-37 3.00888926184257E-4 6.686420581872379E-5 0.0 0.05421015486753031 2.6745682327489515E-4 38-39 3.343210290936189E-4 1.0029630872808567E-4 0.0 0.08872880112144646 2.8417287472957606E-4 40-41 3.343210290936189E-4 1.0029630872808567E-4 0.0 0.13086996683869712 3.343210290936189E-4 42-43 3.6775313200298077E-4 1.1701236018276663E-4 0.0 0.17521765134796569 3.343210290936189E-4 44-45 4.011852349123427E-4 1.3372841163744758E-4 0.0 0.2320689423453356 3.343210290936189E-4 46-47 4.346173378217046E-4 1.3372841163744758E-4 0.0 0.29926746919315295 3.343210290936189E-4 48-49 4.680494407310665E-4 1.3372841163744758E-4 0.0 0.3696921939717238 3.343210290936189E-4 50-51 4.680494407310665E-4 1.3372841163744758E-4 0.0 0.44404519084214467 3.8446918345766174E-4 52-53 4.680494407310665E-4 1.3372841163744758E-4 0.0 0.5261544355875374 4.1790128636702366E-4 54-55 4.680494407310665E-4 1.3372841163744758E-4 0.0 0.6257152380516171 4.346173378217046E-4 56-57 4.680494407310665E-4 1.3372841163744758E-4 0.0 0.7481937470600644 4.6804944073106645E-4 58-59 4.680494407310665E-4 1.3372841163744758E-4 0.0 0.878679244715304 5.014815436404284E-4 60-61 4.847654921857474E-4 1.3372841163744758E-4 0.0 1.0158511629524158 5.014815436404284E-4 62-63 5.349136465497903E-4 1.3372841163744758E-4 0.0 1.1645237245903481 5.014815436404284E-4 64-65 5.349136465497903E-4 1.3372841163744758E-4 0.0 1.336849499036654 5.014815436404284E-4 66-67 5.349136465497903E-4 1.3372841163744758E-4 0.0 1.5420390306428624 5.014815436404284E-4 68-69 5.349136465497903E-4 1.3372841163744758E-4 0.0 1.7705976021827152 5.014815436404284E-4 70-71 5.349136465497903E-4 1.3372841163744758E-4 0.0 2.013398249561956 5.014815436404284E-4 72-73 5.516296980044712E-4 1.3372841163744758E-4 0.0 2.2650082560578135 6.01777852368514E-4 74-75 5.850618009138331E-4 1.3372841163744758E-4 0.0 2.548479056626293 6.01777852368514E-4 76-77 6.01777852368514E-4 1.3372841163744758E-4 0.0 2.8743417636838435 6.01777852368514E-4 78-79 6.01777852368514E-4 1.3372841163744758E-4 0.0 3.231196030138372 6.01777852368514E-4 80-81 6.686420581872378E-4 1.3372841163744758E-4 0.0 3.6140437566049295 6.18493903823195E-4 82-83 6.686420581872378E-4 1.3372841163744758E-4 0.0 4.009545534022681 6.686420581872378E-4 84-85 6.853581096419188E-4 1.3372841163744758E-4 0.0 4.441973069103822 6.686420581872378E-4 86-87 7.020741610965997E-4 1.3372841163744758E-4 0.0 4.919249770237872 6.686420581872378E-4 88-89 7.020741610965997E-4 1.504444630921285E-4 0.0 5.440121933565731 6.686420581872378E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3000 0.0 52.44292 1 GTATCAA 5705 0.0 41.990887 1 TCAACGC 6510 0.0 35.897087 4 ATCAACG 6500 0.0 35.879242 3 CAACGCA 6695 0.0 34.90458 5 TATCAAC 6835 0.0 34.746723 2 AACGCAG 7010 0.0 33.2006 6 ACGCAGA 7980 0.0 29.16494 7 CGCAGAG 8510 0.0 27.40437 8 GTACATG 7500 0.0 24.145924 1 GCAGAGT 9970 0.0 23.200731 9 TACATGG 7965 0.0 22.482157 2 ACATGGG 8010 0.0 21.466002 3 GAGTACT 5790 0.0 19.853678 12-13 CATGGGG 6650 0.0 19.642021 4 CAGAGTA 9515 0.0 19.592901 10-11 GTACTTT 6000 0.0 19.35672 14-15 GTGGTAT 1435 0.0 19.211277 1 AGAGTAC 9295 0.0 19.085745 10-11 TATAACG 175 9.945521E-5 18.999508 2 >>END_MODULE