FastQCFastQC Report
Fri 27 May 2016
SRR940219_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940219_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163099
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA50250.037.0637661
GGTATCA34650.033.868291
TCAACGC55150.033.5988044
ATCAACG56450.032.7416733
CAACGCA58300.031.7815255
AACGCAG62700.029.9200366
TATCAAC63300.028.3735522
ACGCAGA72600.025.906697
GTACATG98300.023.8282931
CGCAGAG78850.023.732748
GTACCTG42450.023.392041
TACCTGG42450.022.9457262
TACATGG101250.022.619222
ACATGGG104400.022.118263
CATGGGG92550.019.1999424
GCAGAGT98800.018.5081239
AGAGTAC85700.017.70874610-11
CAGAGTA88650.016.87833410-11
GTATAAC6850.016.6463971
TATAAGC18800.015.922013
Produced by FastQC (version 0.11.5)