FastQCFastQC Report
Fri 27 May 2016
SRR940219_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940219_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4163099
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51700.034.7401541
GGTATCA34900.032.2666131
ATCAACG54700.031.1765463
TCAACGC55700.030.531544
CAACGCA58050.029.2944985
AACGCAG62050.027.633726
TATCAAC63300.027.316752
GTACATG98100.026.6878191
TACATGG100400.025.4080832
ACATGGG99200.025.0444183
ACGCAGA72100.023.71577
TATCACG2503.8380676E-1022.801982
TATAACG2103.44844E-822.6210142
CGCAGAG78050.021.9686348
CATGGGG87850.021.7913514
GTACCTG39500.020.9306451
TACCTGG39200.020.3589122
CCTATAA19700.018.8130361
GCAGAGT100150.016.978569
TATAAGC20250.016.889953
Produced by FastQC (version 0.11.5)