##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940219_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4163099 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 33.02894310224186 34.0 33.0 34.0 31.0 34.0 2 33.088663757455684 34.0 33.0 34.0 31.0 34.0 3 33.14974998192452 34.0 33.0 34.0 31.0 34.0 4 36.472529478640794 37.0 37.0 37.0 35.0 37.0 5 36.44685485500105 37.0 37.0 37.0 35.0 37.0 6 36.46898092022313 37.0 37.0 37.0 35.0 37.0 7 36.446042239206896 37.0 37.0 37.0 35.0 37.0 8 36.44041157800955 37.0 37.0 37.0 35.0 37.0 9 38.284255550972965 39.0 39.0 39.0 37.0 39.0 10-11 38.24303241407423 39.0 39.0 39.0 37.0 39.0 12-13 38.203723716394926 39.0 39.0 39.0 37.0 39.0 14-15 39.72611496868079 41.0 40.0 41.0 37.0 41.0 16-17 39.70351569828149 41.0 40.0 41.0 37.0 41.0 18-19 39.66019640657116 41.0 40.0 41.0 37.0 41.0 20-21 39.59625797993274 41.0 40.0 41.0 37.0 41.0 22-23 39.52643475449419 41.0 39.5 41.0 37.0 41.0 24-25 39.455252685559486 41.0 39.0 41.0 36.5 41.0 26-27 39.28503778074939 40.5 39.0 41.0 36.0 41.0 28-29 39.423587932931696 41.0 39.0 41.0 36.5 41.0 30-31 39.328561607590885 41.0 39.0 41.0 36.0 41.0 32-33 39.21901400855468 41.0 39.0 41.0 36.0 41.0 34-35 39.35993895413009 41.0 39.0 41.0 36.0 41.0 36-37 39.340072863989064 41.0 39.0 41.0 36.0 41.0 38-39 39.259488184162805 41.0 39.0 41.0 35.5 41.0 40-41 39.14668135444293 41.0 39.0 41.0 35.0 41.0 42-43 39.044088069969035 41.0 39.0 41.0 35.0 41.0 44-45 38.926040793168745 41.0 39.0 41.0 35.0 41.0 46-47 38.76842071255092 40.5 38.5 41.0 35.0 41.0 48-49 38.61730456085719 40.0 38.0 41.0 35.0 41.0 50-51 38.437161955552824 40.0 38.0 41.0 34.5 41.0 52-53 38.2462912844494 40.0 38.0 41.0 34.0 41.0 54-55 38.045417968681505 40.0 37.0 41.0 34.0 41.0 56-57 37.829615509984265 40.0 37.0 41.0 34.0 41.0 58-59 37.537533457647775 39.5 36.0 41.0 33.0 41.0 60-61 37.212795083662435 39.0 35.5 41.0 33.0 41.0 62-63 36.89282647373987 39.0 35.0 40.5 32.5 41.0 64-65 36.53291634429064 38.0 35.0 40.0 32.0 41.0 66-67 36.12772636442227 37.0 35.0 40.0 32.0 41.0 68-69 35.696275659070324 37.0 35.0 39.0 31.0 41.0 70-71 35.29333340379367 36.0 35.0 39.0 31.0 40.5 72-73 34.86081702597032 35.5 34.5 38.5 30.5 40.0 74-75 34.44173114787806 35.0 34.0 37.0 30.0 39.0 76-77 33.11425262766991 34.5 32.5 36.0 28.5 38.5 78-79 33.74143636747529 35.0 34.0 36.0 30.0 38.5 80-81 33.578424269996944 35.0 34.0 36.0 30.0 37.0 82-83 33.33525229642629 35.0 34.0 35.5 29.5 37.0 84-85 33.08328146892495 35.0 34.0 35.0 29.0 36.5 86-87 32.82431284963437 35.0 33.5 35.0 29.0 36.0 88-89 32.58580891302368 35.0 33.0 35.0 29.0 36.0 90-91 32.36115139226811 35.0 33.0 35.0 28.5 35.5 92-93 32.13284202465519 35.0 33.0 35.0 27.0 35.0 94-95 31.89350193209434 35.0 33.0 35.0 27.0 35.0 96-97 31.58132319217006 34.5 33.0 35.0 25.5 35.0 98-99 31.175312309411808 34.0 32.0 35.0 25.0 35.0 100-101 28.720744450228064 32.0 28.5 34.5 12.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1330350198073731 1101 2 0.08359785003559494 1101 3 0.0745094747734143 1101 4 0.046361988880136096 1101 5 0.053076198422651544 1101 6 0.04327516280287114 1101 7 0.011976409812902489 1101 8 -0.035268779307877196 1101 9 -0.0043305217713225375 1101 10-11 0.0209451740540203 1101 12-13 0.0743165284701135 1101 14-15 0.0879197741662665 1101 16-17 0.07885363927857725 1101 18-19 0.08806438765546432 1101 20-21 0.1487146699481272 1101 22-23 0.09867773110937605 1101 24-25 0.023290396240916778 1101 26-27 0.12091017451108854 1101 28-29 0.08104952904424323 1101 30-31 0.012706556140294367 1101 32-33 0.06717541206719346 1101 34-35 0.0668411329768972 1101 36-37 0.04818454184018606 1101 38-39 0.08727200490508835 1101 40-41 0.09965633001963425 1101 42-43 0.0805934976076017 1101 44-45 0.09986289266534953 1101 46-47 0.06495638040104268 1101 48-49 0.09901771806392645 1101 50-51 0.07012226066236593 1101 52-53 0.05150760960661671 1101 54-55 0.13747224217888032 1101 56-57 0.14226937847907806 1101 58-59 0.10732326803643133 1101 60-61 0.21497769614231288 1101 62-63 0.14648546480211166 1101 64-65 0.07812381104860577 1101 66-67 0.06055205585987977 1101 68-69 0.1438412400604463 1101 70-71 0.1132076244068756 1101 72-73 0.025737046687041243 1101 74-75 0.07018929058301637 1101 76-77 -0.10210874921667568 1101 78-79 0.0523871756937524 1101 80-81 0.02357301419294089 1101 82-83 0.04740302208329439 1101 84-85 0.0955385154816426 1101 86-87 0.10162353086978726 1101 88-89 0.0934603023758811 1101 90-91 0.14179717988401563 1101 92-93 0.1597395155345538 1101 94-95 0.12485324198631886 1101 96-97 0.09137524513849016 1101 98-99 0.04265094243020684 1101 100-101 0.23536830385905816 1102 1 0.08733281361727308 1102 2 0.05259590921805568 1102 3 0.06979782282780178 1102 4 0.04491413677919098 1102 5 0.013921687504790725 1102 6 0.05542924852591824 1102 7 0.02673557603192478 1102 8 0.024791928755135473 1102 9 0.05374446551995504 1102 10-11 0.046874712565198706 1102 12-13 0.05226834785489842 1102 14-15 0.1091356511735384 1102 16-17 0.0960997378250994 1102 18-19 0.06989742634597462 1102 20-21 0.08520334241452332 1102 22-23 0.08549953290427936 1102 24-25 0.05997391270737751 1102 26-27 0.10398581317642908 1102 28-29 0.04219118544137501 1102 30-31 0.03798492847015211 1102 32-33 0.10864285528261775 1102 34-35 0.0820900077628437 1102 36-37 0.027839150048393435 1102 38-39 0.0652778051832712 1102 40-41 0.13357060586628933 1102 42-43 0.07719367145518419 1102 44-45 0.07959081510360022 1102 46-47 0.019625873199615285 1102 48-49 0.06769494087100014 1102 50-51 0.06257345888575117 1102 52-53 0.02262817811480744 1102 54-55 0.019352623290913584 1102 56-57 0.06388981421783768 1102 58-59 0.11661345601466877 1102 60-61 0.06990761383634947 1102 62-63 -0.024498980385175173 1102 64-65 0.037054953325885265 1102 66-67 0.08403443743868166 1102 68-69 0.14930134852670562 1102 70-71 0.04601832013535301 1102 72-73 0.016723900572472417 1102 74-75 0.10401043379717123 1102 76-77 -0.025041565109525266 1102 78-79 0.1155336644549152 1102 80-81 0.19476631485224516 1102 82-83 0.16562623716590252 1102 84-85 0.21153015072989945 1102 86-87 0.25389912696020644 1102 88-89 0.16607513685202946 1102 90-91 0.19482803727522224 1102 92-93 0.2049344121989094 1102 94-95 0.15512134755215712 1102 96-97 0.13067070231716116 1102 98-99 0.11925565095001289 1102 100-101 0.23198233672933455 1103 1 0.030802547019405324 1103 2 0.03626676331187895 1103 3 0.06708709360738396 1103 4 0.023565805933188244 1103 5 0.058374916719856174 1103 6 0.1055201949900848 1103 7 -0.011704962431096533 1103 8 0.04932472408223276 1103 9 0.004798513092609369 1103 10-11 0.03601747656688303 1103 12-13 0.05964349586370332 1103 14-15 0.04565352036050996 1103 16-17 0.039055053616486646 1103 18-19 0.07439737602832963 1103 20-21 0.11582457260596613 1103 22-23 0.12365168168177831 1103 24-25 0.09805941737192825 1103 26-27 0.12861201732047078 1103 28-29 0.09901278584220563 1103 30-31 0.07206643341734065 1103 32-33 0.07441173564010484 1103 34-35 0.06122999894120085 1103 36-37 0.040355931517794374 1103 38-39 0.0031713801554857923 1103 40-41 0.11951298038801639 1103 42-43 0.0322917319776721 1103 44-45 0.08535967940227351 1103 46-47 0.07348221204505023 1103 48-49 0.08644883513543533 1103 50-51 0.05291380956760605 1103 52-53 0.12785160451080202 1103 54-55 0.15136965956030224 1103 56-57 0.09594335754346872 1103 58-59 0.15227019761537264 1103 60-61 0.12792894209366779 1103 62-63 -0.038822780844668614 1103 64-65 0.015168116635543072 1103 66-67 -0.008602351610484504 1103 68-69 0.03242283030636628 1103 70-71 -0.23457775123433322 1103 72-73 -0.013034435562232716 1103 74-75 0.06259272348547995 1103 76-77 0.016527767250281045 1103 78-79 0.07853253177264463 1103 80-81 0.1983831631263513 1103 82-83 0.10530568701205567 1103 84-85 0.09185311506004012 1103 86-87 0.1194807614485569 1103 88-89 0.02696371431872535 1103 90-91 0.05264175172366947 1103 92-93 0.11972710350766391 1103 94-95 0.06716945228993865 1103 96-97 0.10748604513019444 1103 98-99 0.12762256506528757 1103 100-101 0.06774153361692115 1104 1 -0.0432542689378792 1104 2 -0.03145911979077454 1104 3 -0.016005879473659945 1104 4 0.003050147287090965 1104 5 -0.024125572677910156 1104 6 -0.04734007465767576 1104 7 -0.014029081202728833 1104 8 0.006243142515245381 1104 9 4.361046724667972E-4 1104 10-11 -0.02070632815716067 1104 12-13 -0.0521635897829853 1104 14-15 -0.011550831322814759 1104 16-17 -0.05102068849795671 1104 18-19 0.045672590426207194 1104 20-21 0.02928056429244208 1104 22-23 -0.007742508437800666 1104 24-25 -0.01378723660510417 1104 26-27 0.011617315311646337 1104 28-29 -0.04024326707106951 1104 30-31 0.04800185998476536 1104 32-33 0.03441466161400797 1104 34-35 0.047838722300255654 1104 36-37 -0.011948455152264614 1104 38-39 -0.04738445075862785 1104 40-41 -0.006764920491242776 1104 42-43 0.013733143775702672 1104 44-45 -0.07148527730164744 1104 46-47 -0.24865918434215928 1104 48-49 -0.07565766823204711 1104 50-51 -0.060788911483996344 1104 52-53 -0.09237921422042206 1104 54-55 -0.09038138483536784 1104 56-57 -0.08464337831918556 1104 58-59 -0.17548755881864508 1104 60-61 -0.10762991892152485 1104 62-63 -0.1434010969831263 1104 64-65 -0.16578176278517986 1104 66-67 -0.0844603075521917 1104 68-69 -0.06566966378962746 1104 70-71 -0.10207096214186606 1104 72-73 -0.11276120582850524 1104 74-75 -0.08956312575502068 1104 76-77 -0.2401233566985539 1104 78-79 -0.15406104732861792 1104 80-81 -0.06634823038694293 1104 82-83 -0.11323176337133845 1104 84-85 -0.10661668389118972 1104 86-87 -0.11758898502256443 1104 88-89 -0.15508626881268128 1104 90-91 -0.18689711062435777 1104 92-93 -0.11951838049631647 1104 94-95 -0.10476431449015777 1104 96-97 -0.17708522159783868 1104 98-99 -0.2018712518624568 1104 100-101 -0.14374989046811493 1105 1 0.029522560943448184 1105 2 0.01584211060907137 1105 3 -0.001856445110696825 1105 4 0.02871822521023404 1105 5 0.03314237294308242 1105 6 0.006646661850808755 1105 7 0.040991391848017145 1105 8 -0.004434116894266538 1105 9 0.007387387332670414 1105 10-11 0.06303777080928086 1105 12-13 0.03309392922914611 1105 14-15 0.06975983972233024 1105 16-17 0.03496990615076356 1105 18-19 -0.06012806316856256 1105 20-21 -0.04392020345326131 1105 22-23 0.0737082672490601 1105 24-25 0.037094099112081835 1105 26-27 0.07950768856603929 1105 28-29 -0.00495029017704951 1105 30-31 0.04214910359768709 1105 32-33 0.0036438881859695016 1105 34-35 0.016593275858681977 1105 36-37 0.11139464832122314 1105 38-39 0.019345001442943044 1105 40-41 0.019879698759432074 1105 42-43 -0.1527938909369695 1105 44-45 -0.13199085022689871 1105 46-47 -0.021439486902636418 1105 48-49 -0.06087955919471 1105 50-51 -0.17071868143254676 1105 52-53 0.02481658538821563 1105 54-55 0.07923652339918874 1105 56-57 0.039721171815713774 1105 58-59 -0.02916792435373594 1105 60-61 -0.1039673768649294 1105 62-63 0.09043128114284826 1105 64-65 -0.030538462219816154 1105 66-67 -0.014240970363744054 1105 68-69 -0.12477926381261284 1105 70-71 -0.12677283484239865 1105 72-73 -0.18326365145177448 1105 74-75 -0.15724756472384627 1105 76-77 -0.30130339198248635 1105 78-79 -0.2577232301425525 1105 80-81 -0.1428566777577629 1105 82-83 -0.21186426517763834 1105 84-85 -0.13422527097589665 1105 86-87 -0.2070266706627919 1105 88-89 -0.28032226603755817 1105 90-91 -0.2934626359833601 1105 92-93 -0.27838837826271146 1105 94-95 -0.3576718797122389 1105 96-97 -0.44816444708576597 1105 98-99 -0.40458546259496586 1105 100-101 -0.1495922162127279 1106 1 -0.09084986301727582 1106 2 -0.04244568651525782 1106 3 -0.04597170764962755 1106 4 0.013560243367010116 1106 5 -0.005816341469319752 1106 6 -0.03573184998715817 1106 7 -0.026954975688880722 1106 8 0.008494390619077308 1106 9 -0.08259286575663793 1106 10-11 -0.04137414890562496 1106 12-13 -0.0045903511682894305 1106 14-15 0.004600798295506081 1106 16-17 -0.022916595258138273 1106 18-19 -0.025029787998086306 1106 20-21 -0.027036653485126294 1106 22-23 -0.1761434428187698 1106 24-25 -0.06281403809646235 1106 26-27 -0.04230117870596217 1106 28-29 -0.019139391541791895 1106 30-31 -0.010467643359191925 1106 32-33 -0.04761862810210715 1106 34-35 -0.10416108139411762 1106 36-37 -0.18875514987814768 1106 38-39 -0.15392214622635692 1106 40-41 -0.14146595998162326 1106 42-43 -0.031917595285399614 1106 44-45 -0.1649210862075563 1106 46-47 -0.00574305021668664 1106 48-49 -0.02444527681270614 1106 50-51 -0.03227756152654848 1106 52-53 -0.07880596180518751 1106 54-55 -0.02182583513173597 1106 56-57 0.03596034033458295 1106 58-59 -0.058214769200255034 1106 60-61 -0.06123423240668302 1106 62-63 -0.09356868903694959 1106 64-65 -0.043160208202358774 1106 66-67 -0.07550671510294649 1106 68-69 -0.08351918215081611 1106 70-71 -0.05548108233048055 1106 72-73 0.013474998561413543 1106 74-75 -0.07979083042123136 1106 76-77 -0.26576968662832456 1106 78-79 -0.11114292057433062 1106 80-81 -0.019289695542276775 1106 82-83 -0.030443915111924014 1106 84-85 -0.0075802501870114725 1106 86-87 0.089850153192927 1106 88-89 0.0505625663373479 1106 90-91 0.1273355611092697 1106 92-93 0.023479740095609714 1106 94-95 -0.032105639274291775 1106 96-97 0.0683590515446788 1106 98-99 -0.03416428743461353 1106 100-101 -0.01959218100832416 1107 1 0.0593630476181346 1107 2 0.0791068341800667 1107 3 0.05955976698327703 1107 4 0.03905106923170365 1107 5 0.05939654832015151 1107 6 0.06661681726641433 1107 7 0.0701441930322062 1107 8 0.09630836830509537 1107 9 0.052981125604311785 1107 10-11 0.09934310843556204 1107 12-13 0.043836691756446555 1107 14-15 0.09482293182937696 1107 16-17 0.16988804794232237 1107 18-19 0.16847233587634491 1107 20-21 0.17634338102430291 1107 22-23 0.13873621846656192 1107 24-25 0.18886551122572826 1107 26-27 0.17037144510400992 1107 28-29 0.15913759248702775 1107 30-31 0.1102737249700354 1107 32-33 0.15628533440069958 1107 34-35 0.12007347811928781 1107 36-37 0.11036538846705923 1107 38-39 0.19590704376494017 1107 40-41 0.2049589651589656 1107 42-43 0.27695209935098575 1107 44-45 0.19660463886946644 1107 46-47 0.14183485167936283 1107 48-49 0.12906717847376825 1107 50-51 0.04382075185694134 1107 52-53 0.21983279004774658 1107 54-55 0.22608382787768022 1107 56-57 0.2155330018062216 1107 58-59 0.24493847781671008 1107 60-61 0.1701385496771195 1107 62-63 0.22984861147577362 1107 64-65 0.157033462855388 1107 66-67 0.19745694911325273 1107 68-69 0.14780970083290157 1107 70-71 0.06141085811356817 1107 72-73 0.20056008768844436 1107 74-75 0.06611440948816494 1107 76-77 -0.07997414378488799 1107 78-79 0.24484465099367725 1107 80-81 0.29099065620969355 1107 82-83 0.2587618030197163 1107 84-85 0.25532565605677604 1107 86-87 0.30536191247020383 1107 88-89 0.2836979749509112 1107 90-91 0.30481214273784474 1107 92-93 0.38187636674815195 1107 94-95 0.40606088415487207 1107 96-97 0.4526692270536081 1107 98-99 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2316 60-61 -0.3669493732161655 2316 62-63 -0.32654881196312147 2316 64-65 -0.5149482742238263 2316 66-67 -0.532586158284424 2316 68-69 -0.4607763359732431 2316 70-71 -0.39829854918851737 2316 72-73 -0.5013167149853075 2316 74-75 -0.4193558448231016 2316 76-77 -0.4549901672437784 2316 78-79 -0.5929915466564282 2316 80-81 -0.6122219738988548 2316 82-83 -0.6924565005843064 2316 84-85 -0.5762312291888847 2316 86-87 -0.6657617095261799 2316 88-89 -0.49762651612907405 2316 90-91 -0.5243683679941853 2316 92-93 -0.6030859840785716 2316 94-95 -0.4957045935646853 2316 96-97 -0.6763357374765171 2316 98-99 -0.5241639289426345 2316 100-101 -0.6317325223616699 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 4.0 4 12.0 5 47.0 6 56.0 7 166.0 8 380.0 9 714.0 10 1175.0 11 1852.0 12 2882.0 13 3881.0 14 4364.0 15 5488.0 16 7277.0 17 10230.0 18 9912.0 19 7846.0 20 8058.0 21 9057.0 22 9369.0 23 10619.0 24 12440.0 25 15219.0 26 19133.0 27 23845.0 28 30188.0 29 38410.0 30 47867.0 31 60709.0 32 78341.0 33 105555.0 34 153211.0 35 246434.0 36 494206.0 37 1160535.0 38 1398054.0 39 185550.0 40 6.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.15867493789372 18.217225422971396 13.753645890894765 24.87045374824012 2 16.07914700754593 20.17637119814904 37.923410739945204 25.821071054359823 3 18.451970455478442 24.282375062844203 29.008090898237914 28.257563583439442 4 13.749441207055217 17.022520586760816 35.764670161429606 33.46336804475436 5 15.450602226718216 35.81431651147159 33.55413458226092 15.180946679549276 6 33.197919696799005 34.71226724079742 17.988795226188895 14.101017836214677 7 29.80227403476028 30.667882111463012 21.380514690427894 18.149329163348817 8 27.336967004628043 33.61959444154463 20.219096399100767 18.824342154726565 9 26.982303327401052 16.202857534735543 19.505373280817967 37.30946585704544 10-11 25.706150634419217 25.418348206468306 27.324200073070564 21.551301086041914 12-13 26.33954897541471 22.485605074488983 27.571227587909874 23.603618362186438 14-15 23.70844411819176 23.836894102206074 24.64182331479506 27.812838464807104 16-17 24.29232405955275 26.793249451910704 25.260449006857634 23.65397748167891 18-19 24.30557140245764 26.161352396375875 26.29961478216108 23.23346141900541 20-21 24.68184206654476 25.642414251784324 26.01924699509667 23.656496686574243 22-23 24.61675766862987 25.27522178801022 25.998145130896543 24.109875412463367 24-25 24.594119081942925 25.538192986937897 25.773151549413782 24.094536381705396 26-27 24.371406434677564 25.823347045525267 25.709681297528626 24.09556522226854 28-29 24.4062785658086 25.715175850982607 25.91064389877265 23.967901684436143 30-31 24.49160628980294 25.47877026365648 25.73577903419037 24.29384441235021 32-33 24.334755525748392 25.53768193365643 25.77612455849741 24.351437982097767 34-35 24.735103072087348 25.540600153996508 25.515630669253053 24.208666104663095 36-37 24.62767843330928 25.39858825270209 25.652103208499117 24.321630105489508 38-39 24.430898712713773 25.552274879843118 25.86029061523639 24.156535792206718 40-41 24.565037158136676 25.337156779508852 25.889619610262628 24.208186452091844 42-43 24.54474419176676 25.36551496853666 25.683955630168775 24.405785209527807 44-45 24.53773180067241 25.49105093060161 25.52657745641365 24.44463981231233 46-47 24.563151399410867 25.558932598271046 25.471878162224165 24.406037840093926 48-49 24.68903125817462 25.533882819738178 25.44556993100473 24.331515991082476 50-51 24.438933592499243 25.590143304302877 25.522609479140417 24.448313624057462 52-53 24.777365210891126 25.48863874385794 25.291501805387146 24.442494239863795 54-55 24.574024121928627 25.368632220757885 25.478827645587977 24.57851601172551 56-57 24.48537735951031 25.697947610662155 25.604927963519486 24.211747066308057 58-59 24.679156588015154 25.34553660862484 25.53289743916178 24.442409364198227 60-61 24.709708266431008 25.377000028704593 25.639556998018183 24.273734706846213 62-63 24.609421399243086 25.5158642511992 25.520968637091734 24.35374571246598 64-65 24.507810995867292 25.335769711791436 25.538591732735654 24.61782755960562 66-67 24.419646336918536 25.59800282794989 25.460304512048932 24.522046323082648 68-69 24.26355872253696 25.427152154827116 25.7017315242626 24.607557598373326 70-71 24.327177902807502 25.440315015328725 25.578817606787634 24.653689475076142 72-73 24.484701753003705 25.405390213753172 25.48213727226301 24.627770760980113 74-75 24.405721370418416 25.490131317012217 25.362113534082287 24.74203377848708 76-77 24.48968267846814 25.39485037097391 25.48101220822196 24.634454742335993 78-79 24.36774864109645 25.523906589778434 25.510515123469318 24.597829645655796 80-81 24.375339140385563 25.603474719193564 25.509986670987168 24.51119946943371 82-83 24.481951691020846 25.479639218779766 25.406304039055726 24.63210505114366 84-85 24.368517799010707 25.386945850820876 25.37601644934973 24.868519900818683 86-87 24.472375539646976 25.32526547718635 25.594552996050346 24.607805987116333 88-89 24.317836495022398 25.30575498309972 25.531800668084443 24.844607853793445 90-91 24.25913188167729 25.547781986642686 25.60002747992842 24.593058651751598 92-93 24.350963351655665 25.325184932865895 25.606649944197045 24.717201771281392 94-95 24.327682334722283 25.281406951888485 25.516976656092012 24.87393405729722 96-97 24.49086435328048 25.259085233087532 25.469447297246823 24.78060311638517 98-99 24.17434624960516 25.280386347176798 25.47100174269384 25.074265660524205 100-101 24.06499405085206 25.186419847693166 25.748784286274006 24.999801815180763 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 59.0 1 57.0 2 159.5 3 227.5 4 137.5 5 110.0 6 199.0 7 411.5 8 647.5 9 771.0 10 762.5 11 747.0 12 823.5 13 914.5 14 1044.5 15 1144.0 16 1157.0 17 1163.5 18 1196.0 19 1262.0 20 1367.5 21 1577.5 22 1877.0 23 2366.5 24 3005.5 25 3917.0 26 5365.5 27 7517.0 28 10167.5 29 13523.5 30 18247.0 31 23520.5 32 28714.5 33 34773.0 34 41645.0 35 48141.0 36 55928.0 37 65185.5 38 75467.0 39 83322.0 40 89607.0 41 102501.5 42 115901.5 43 127278.0 44 140007.5 45 151250.0 46 159557.5 47 167839.5 48 181932.0 49 192802.0 50 197066.0 51 199152.0 52 197240.5 53 195978.5 54 193804.5 55 183087.5 56 168233.5 57 154433.0 58 138957.0 59 121316.0 60 101797.5 61 81172.5 62 63857.0 63 50144.0 64 38882.5 65 29282.0 66 21275.0 67 16923.5 68 12897.0 69 8826.0 70 6297.0 71 4439.0 72 3163.5 73 2357.0 74 1842.0 75 1451.0 76 945.5 77 481.0 78 240.0 79 119.5 80 65.0 81 34.0 82 19.5 83 18.5 84 13.5 85 9.5 86 5.0 87 1.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021114078718762152 2 0.0014652546096069299 3 6.245347516357406E-4 4 0.002594221276025384 5 0.00915183616820066 6 0.0012490695032714811 7 3.362879431884757E-4 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.002678293261822503 22-23 4.804113474121082E-5 24-25 5.885039005798325E-4 26-27 3.723187942443838E-4 28-29 3.4829822687377837E-4 30-31 2.762365247619622E-4 32-33 0.0010328843969360324 34-35 4.2035992898559465E-4 36-37 7.446375884887676E-4 38-39 0.0 40-41 3.24277659503173E-4 42-43 0.0 44-45 3.002570921325676E-4 46-47 0.0044798358146179084 48-49 0.0014412340422363245 50-51 0.0 52-53 7.206170211181622E-5 54-55 9.72832978509519E-4 56-57 0.0 58-59 2.1618510633544867E-4 60-61 6.005141842651352E-5 62-63 3.002570921325676E-4 64-65 0.002149840779669184 66-67 3.002570921325676E-4 68-69 9.608226948242164E-5 70-71 0.0 72-73 0.0017535014180541948 74-75 1.3211312053832975E-4 76-77 7.206170211181622E-5 78-79 0.0 80-81 0.0 82-83 5.28452482153319E-4 84-85 3.9633936161498926E-4 86-87 0.0018375734038513136 88-89 6.005141842651352E-5 90-91 0.0014532443259216272 92-93 0.005692874466833481 94-95 0.0 96-97 9.247918437683082E-4 98-99 9.608226948242164E-5 100-101 0.007638540423852519 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4163099.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.0656084496074 #Duplication Level Percentage of deduplicated Percentage of total 1 75.55086070161049 37.069489492160514 2 12.182402708314974 11.954740025232388 3 4.373338774864384 6.437415838349447 4 2.231521557114252 4.379638518729044 5 1.2698142498098988 3.1152104392452227 6 0.8788947925097915 2.587410465461062 7 0.6111208634942356 2.0989511902515896 8 0.4390953988181148 1.7235586328347061 9 0.3428743616057757 1.5141005256562283 >10 1.9251937309548364 17.071018692389003 >50 0.12415429383057326 4.207341163344356 >100 0.06606848057853507 5.860558961583333 >500 0.003384694509057542 1.0833676806344517 >1k 0.0012753919849838435 0.897198374128667 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 2.4020567370605407E-5 12-13 0.0 1.2010283685302703E-5 0.0 0.0 8.407198579711892E-5 14-15 0.0 2.4020567370605407E-5 0.0 0.0 1.0809255316772434E-4 16-17 0.0 2.4020567370605407E-5 0.0 0.0 1.2010283685302703E-4 18-19 0.0 2.4020567370605407E-5 0.0 2.4020567370605407E-5 1.2010283685302703E-4 20-21 0.0 2.4020567370605407E-5 0.0 2.4020567370605407E-5 1.4412340422363245E-4 22-23 0.0 2.4020567370605407E-5 0.0 3.603085105590811E-5 1.4412340422363245E-4 24-25 0.0 2.4020567370605407E-5 0.0 7.206170211181622E-5 1.4412340422363245E-4 26-27 0.0 2.4020567370605407E-5 0.0 2.522159573913568E-4 1.5613368790893514E-4 28-29 1.2010283685302703E-5 2.4020567370605407E-5 0.0 6.605656026916487E-4 1.6814397159423786E-4 30-31 3.603085105590811E-5 2.4020567370605407E-5 0.0 0.0022819539002075138 1.6814397159423786E-4 32-33 4.804113474121081E-5 2.4020567370605407E-5 0.0 0.005921069856854233 1.6814397159423786E-4 34-35 4.804113474121081E-5 2.4020567370605407E-5 0.0 0.010569049643066378 1.6814397159423786E-4 36-37 4.804113474121081E-5 2.4020567370605407E-5 0.0 0.01817155921586299 1.6814397159423786E-4 38-39 4.804113474121081E-5 2.4020567370605407E-5 0.0 0.030458079425927657 1.6814397159423786E-4 40-41 4.804113474121081E-5 2.4020567370605407E-5 0.0 0.044786347862493775 1.6814397159423786E-4 42-43 9.608226948242163E-5 2.4020567370605407E-5 0.0 0.060399716653387295 1.6814397159423786E-4 44-45 1.4412340422363245E-4 2.4020567370605407E-5 0.0 0.07833107019554422 1.8015425527954056E-4 46-47 1.4412340422363245E-4 2.4020567370605407E-5 0.0 0.10135478402026951 2.0417482265014598E-4 48-49 1.4412340422363245E-4 2.4020567370605407E-5 0.0 0.12601189642619598 2.1618510633544867E-4 50-51 1.4412340422363245E-4 2.4020567370605407E-5 0.0 0.15144967727166708 2.1618510633544867E-4 52-53 1.4412340422363245E-4 2.4020567370605407E-5 0.0 0.1789892577620662 2.4020567370605406E-4 54-55 1.4412340422363245E-4 4.804113474121081E-5 0.0 0.21028805704596504 2.4020567370605406E-4 56-57 1.4412340422363245E-4 4.804113474121081E-5 0.0 0.24684736058402648 2.4020567370605406E-4 58-59 1.4412340422363245E-4 4.804113474121081E-5 0.0 0.2888593329152153 2.4020567370605406E-4 60-61 1.6814397159423786E-4 4.804113474121081E-5 0.0 0.3348467091462394 2.4020567370605406E-4 62-63 1.6814397159423786E-4 4.804113474121081E-5 0.0 0.38369253289436545 2.4020567370605406E-4 64-65 1.6814397159423786E-4 4.804113474121081E-5 0.0 0.4398886502578968 2.4020567370605406E-4 66-67 1.6814397159423786E-4 4.804113474121081E-5 0.0 0.5066898481155504 2.4020567370605406E-4 68-69 1.6814397159423786E-4 4.804113474121081E-5 0.0 0.5804329899433092 2.4020567370605406E-4 70-71 1.6814397159423786E-4 4.804113474121081E-5 0.0 0.6634961119108626 2.4020567370605406E-4 72-73 1.6814397159423786E-4 4.804113474121081E-5 0.0 0.7547262267844219 2.522159573913568E-4 74-75 1.8015425527954056E-4 4.804113474121081E-5 0.0 0.8574381728611307 3.002570921325676E-4 76-77 1.9216453896484325E-4 4.804113474121081E-5 0.0 0.9730251430484838 3.3628794318847573E-4 78-79 1.9216453896484325E-4 4.804113474121081E-5 0.0 1.1063272816716585 3.3628794318847573E-4 80-81 1.9216453896484325E-4 4.804113474121081E-5 0.0 1.2524323827033659 3.3628794318847573E-4 82-83 1.9216453896484325E-4 4.804113474121081E-5 0.0 1.413322142951681 3.843290779296865E-4 84-85 1.9216453896484325E-4 4.804113474121081E-5 0.0 1.5893929017782185 4.083496453002919E-4 86-87 1.9216453896484325E-4 4.804113474121081E-5 0.0 1.7880309836494401 4.3237021267089734E-4 88-89 1.9216453896484325E-4 4.804113474121081E-5 0.0 2.00784319565785 4.3237021267089734E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5170 0.0 34.740154 1 GGTATCA 3490 0.0 32.266613 1 ATCAACG 5470 0.0 31.176546 3 TCAACGC 5570 0.0 30.53154 4 CAACGCA 5805 0.0 29.294498 5 AACGCAG 6205 0.0 27.63372 6 TATCAAC 6330 0.0 27.31675 2 GTACATG 9810 0.0 26.687819 1 TACATGG 10040 0.0 25.408083 2 ACATGGG 9920 0.0 25.044418 3 ACGCAGA 7210 0.0 23.7157 7 TATCACG 250 3.8380676E-10 22.80198 2 TATAACG 210 3.44844E-8 22.621014 2 CGCAGAG 7805 0.0 21.968634 8 CATGGGG 8785 0.0 21.791351 4 GTACCTG 3950 0.0 20.930645 1 TACCTGG 3920 0.0 20.358912 2 CCTATAA 1970 0.0 18.813036 1 GCAGAGT 10015 0.0 16.97856 9 TATAAGC 2025 0.0 16.88995 3 >>END_MODULE