FastQCFastQC Report
Fri 27 May 2016
SRR940218_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940218_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4236053
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48000.037.5942341
ATCAACG55650.033.6231233
GGTATCA32500.033.2256771
TCAACGC56650.033.1126484
CAACGCA58900.031.684615
AACGCAG63200.029.9052456
TATCAAC61650.028.8812182
ACGCAGA72500.025.7428387
GTACATG96550.025.400561
CGCAGAG76700.024.3968358
ACATGGG105700.023.767733
TACATGG102800.023.1559832
GTACCTG39100.020.9891091
TATAACG2551.0604708E-820.702712
CATGGGG91450.019.733164
TATCACG3203.45608E-1019.4970112
TACCTGG42600.018.8140182
GCAGAGT102100.018.18589
AGAGTAC89700.017.36523610-11
ATGGGGA62900.017.212955
Produced by FastQC (version 0.11.5)