##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940218_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4236053 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.35671626393721 33.0 31.0 34.0 28.0 34.0 2 29.5668760518341 33.0 30.0 34.0 16.0 34.0 3 30.925030210906236 33.0 31.0 34.0 25.0 34.0 4 34.99248663791506 37.0 35.0 37.0 32.0 37.0 5 35.298406559124736 37.0 35.0 37.0 33.0 37.0 6 35.524294195563655 37.0 35.0 37.0 35.0 37.0 7 35.58049438947057 37.0 36.0 37.0 35.0 37.0 8 35.614052279327005 37.0 36.0 37.0 35.0 37.0 9 37.367785294471055 39.0 38.0 39.0 35.0 39.0 10-11 37.29753947837763 39.0 38.0 39.0 35.0 39.0 12-13 37.22040186938171 39.0 38.0 39.0 35.0 39.0 14-15 38.61278813083783 41.0 39.0 41.0 35.0 41.0 16-17 38.545875724406656 41.0 39.0 41.0 34.5 41.0 18-19 38.47276072797011 41.0 39.0 41.0 34.0 41.0 20-21 38.379762009587694 40.0 39.0 41.0 34.0 41.0 22-23 38.26466713235175 40.0 38.0 41.0 34.0 41.0 24-25 38.14592900513757 40.0 38.0 41.0 34.0 41.0 26-27 38.00229789381767 40.0 38.0 41.0 33.0 41.0 28-29 37.83206442412312 40.0 38.0 41.0 33.0 41.0 30-31 37.67195016209665 40.0 38.0 41.0 33.0 41.0 32-33 37.75875903818956 40.0 38.0 41.0 33.0 41.0 34-35 37.80618833144911 40.0 38.0 41.0 33.0 41.0 36-37 37.90525047727212 40.0 38.0 41.0 33.0 41.0 38-39 37.84012003626961 40.0 38.0 41.0 33.0 41.0 40-41 37.71194517632334 40.0 38.0 41.0 33.0 41.0 42-43 37.46958099910459 40.0 38.0 41.0 32.0 41.0 44-45 37.4227134315836 40.0 38.0 41.0 32.0 41.0 46-47 37.24998081940901 40.0 37.5 41.0 32.0 41.0 48-49 37.06097716435559 40.0 37.0 41.0 31.0 41.0 50-51 36.161392102506746 39.0 36.0 40.0 30.0 40.5 52-53 36.26281233969452 39.0 36.0 40.0 30.5 41.0 54-55 36.572875858729816 40.0 36.0 41.0 31.0 41.0 56-57 36.41460907122739 39.5 36.0 41.0 30.5 41.0 58-59 36.15529255653789 39.0 35.0 41.0 30.0 41.0 60-61 35.84517615808868 39.0 35.0 41.0 29.5 41.0 62-63 35.4952204327944 38.0 35.0 40.5 29.0 41.0 64-65 35.105307936421006 37.5 35.0 40.0 28.0 41.0 66-67 34.729266253278695 37.0 35.0 39.5 28.0 41.0 68-69 34.34204989880911 36.5 34.0 39.0 28.0 41.0 70-71 33.91619179457859 36.0 34.0 39.0 27.5 40.5 72-73 33.48258579389824 35.0 34.0 37.5 26.0 40.0 74-75 33.04105224840199 35.0 34.0 37.0 26.0 39.0 76-77 32.57153262718856 35.0 33.0 37.0 25.5 39.0 78-79 32.13250459803029 35.0 33.0 36.0 24.5 38.5 80-81 31.784863999576963 35.0 33.0 36.0 24.0 37.0 82-83 31.435215517841726 35.0 32.0 35.0 22.5 37.0 84-85 31.134768615973407 35.0 32.0 35.0 20.5 36.0 86-87 30.851516376211535 35.0 32.0 35.0 20.0 36.0 88-89 30.556169741030153 34.5 31.5 35.0 18.5 36.0 90-91 30.29908159789313 34.0 31.0 35.0 17.0 35.0 92-93 30.0158281777872 34.0 31.0 35.0 9.0 35.0 94-95 29.67561312382069 34.0 31.0 35.0 2.0 35.0 96-97 29.304903172835658 34.0 30.5 35.0 2.0 35.0 98-99 28.84492179394356 34.0 30.0 35.0 2.0 35.0 100-101 26.9468689367201 32.5 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7074797989955535 1101 2 1.8852532970712481 1101 3 1.0271245306857253 1101 4 0.6274437979617389 1101 5 0.4377514313455819 1101 6 0.3645322702672402 1101 7 0.3392082315807414 1101 8 0.3756489817272026 1101 9 0.4037859635307939 1101 10-11 0.34480288145230276 1101 12-13 0.4171180728201591 1101 14-15 0.5193293602435958 1101 16-17 0.5176889689903206 1101 18-19 0.5038476228648321 1101 20-21 0.47783026601837975 1101 22-23 0.5357515781724018 1101 24-25 0.4741471294601425 1101 26-27 0.5827286795554372 1101 28-29 0.5439733717203268 1101 30-31 0.5768865062851276 1101 32-33 0.5747553071980676 1101 34-35 0.6229910522893221 1101 36-37 0.6703509420212086 1101 38-39 0.7175463889554194 1101 40-41 0.7056679643055972 1101 42-43 0.9044640813320655 1101 44-45 0.7867439884254424 1101 46-47 0.7082248055699694 1101 48-49 0.6944004776500634 1101 50-51 0.6671749765004762 1101 52-53 0.6623680876766898 1101 54-55 0.8007194844107843 1101 56-57 0.8372804724021421 1101 58-59 0.8120274407023373 1101 60-61 0.7581342604906993 1101 62-63 0.8012077098278425 1101 64-65 0.813502909966374 1101 66-67 0.8586614288837637 1101 68-69 0.827277335469681 1101 70-71 0.7287265080903111 1101 72-73 0.7210280875569453 1101 74-75 0.6859902275248828 1101 76-77 0.7373675766387393 1101 78-79 0.8366179784907146 1101 80-81 0.8593464534542328 1101 82-83 0.8429207838451873 1101 84-85 0.783313112829898 1101 86-87 0.7115062881688381 1101 88-89 0.8315451652758235 1101 90-91 0.6906529288093672 1101 92-93 0.7603434781292506 1101 94-95 0.6287332488260056 1101 96-97 0.49932924042132854 1101 98-99 0.4911242321769258 1101 100-101 0.4507270897557554 1102 1 0.9478459909537627 1102 2 2.6747553983560195 1102 3 1.4899314510427395 1102 4 0.7847509127036361 1102 5 0.5462885989640611 1102 6 0.42253494496557664 1102 7 0.31167121330305747 1102 8 0.28501410062175836 1102 9 0.3826090626754137 1102 10-11 0.27621024477978295 1102 12-13 0.35958394394758386 1102 14-15 0.39386293898042624 1102 16-17 0.39816265676138585 1102 18-19 0.4060585585745997 1102 20-21 0.3900930195576322 1102 22-23 0.44067086931486443 1102 24-25 0.5125457514134268 1102 26-27 0.42059422272430425 1102 28-29 0.4907153743125292 1102 30-31 0.4859400114707668 1102 32-33 0.47196416604293745 1102 34-35 0.5025539829079335 1102 36-37 0.5576948437741862 1102 38-39 0.5198662030392285 1102 40-41 0.4785272668028071 1102 42-43 0.6221065229244047 1102 44-45 0.4905458369057314 1102 46-47 0.4587715655297373 1102 48-49 0.45847023800678954 1102 50-51 0.3519171305160498 1102 52-53 0.36437126299922085 1102 54-55 0.4618132918600466 1102 56-57 0.6042983194668281 1102 58-59 0.5794952247758403 1102 60-61 0.6052386446003268 1102 62-63 0.6338609368083112 1102 64-65 0.7177367315650542 1102 66-67 0.608774946026081 1102 68-69 0.6207681056644319 1102 70-71 0.6034441260112615 1102 72-73 0.5490893370335357 1102 74-75 0.6163726615541094 1102 76-77 0.6623557042807136 1102 78-79 0.6403565973013272 1102 80-81 0.5989758840573494 1102 82-83 0.6695796204770375 1102 84-85 0.6664369111084412 1102 86-87 0.6008601772805804 1102 88-89 0.5112057686704041 1102 90-91 0.5529615225155418 1102 92-93 0.48316531778614547 1102 94-95 0.36648322250830034 1102 96-97 0.35470406801265497 1102 98-99 0.4384024306983356 1102 100-101 0.4808625908304016 1103 1 0.8309478137416271 1103 2 2.6244365377411967 1103 3 1.370158682749281 1103 4 0.7422765074582145 1103 5 0.43671256239657197 1103 6 0.3667636493714639 1103 7 0.2995924992835768 1103 8 0.2868050002549296 1103 9 0.21438478331193522 1103 10-11 0.19720037716089678 1103 12-13 0.16174034608205545 1103 14-15 0.23317037098448878 1103 16-17 0.2943447308133784 1103 18-19 0.2763841936560425 1103 20-21 0.30590836716570635 1103 22-23 0.23292412474493318 1103 24-25 0.24690010338819945 1103 26-27 0.3275329791527142 1103 28-29 0.334109559419403 1103 30-31 0.3700551831076453 1103 32-33 0.3316919398742115 1103 34-35 0.40626259106257834 1103 36-37 0.3385645647543214 1103 38-39 0.30831252568377465 1103 40-41 0.3919782784372643 1103 42-43 0.4841955325706664 1103 44-45 0.35262018280172214 1103 46-47 0.3505105542468314 1103 48-49 0.36154098852481553 1103 50-51 0.20641530876983438 1103 52-53 0.31082067302973826 1103 54-55 0.5001561467105589 1103 56-57 0.5859109066564585 1103 58-59 0.576887340340825 1103 60-61 0.5430877185860439 1103 62-63 0.5432333797587248 1103 64-65 0.5328495646282789 1103 66-67 0.5012793639071234 1103 68-69 0.40945044338492664 1103 70-71 0.4548043547087204 1103 72-73 0.5334145010148745 1103 74-75 0.5045562643563315 1103 76-77 0.5759124933273867 1103 78-79 0.7143407667753436 1103 80-81 0.5884186968883149 1103 82-83 0.5666498279712755 1103 84-85 0.49135120567323654 1103 86-87 0.5425547190234994 1103 88-89 0.5593790405040302 1103 90-91 0.5266017107894179 1103 92-93 0.3197054862004016 1103 94-95 0.25445988972245814 1103 96-97 0.3105106248109415 1103 98-99 0.22174232706747432 1103 100-101 0.31051901203084853 1104 1 0.9156352509211523 1104 2 2.65607915517554 1104 3 1.4258341122536855 1104 4 0.8928889184342736 1104 5 0.5762267840176207 1104 6 0.45363357054385034 1104 7 0.37349648898145915 1104 8 0.3234983761730774 1104 9 0.28551442479723477 1104 10-11 0.2886205149182146 1104 12-13 0.3707042277212196 1104 14-15 0.41318795658163054 1104 16-17 0.39610876802828443 1104 18-19 0.42085417526475055 1104 20-21 0.4215660799560581 1104 22-23 0.46256024282391905 1104 24-25 0.4962205777054933 1104 26-27 0.452294311131503 1104 28-29 0.4292834703877091 1104 30-31 0.45604769314559235 1104 32-33 0.4643031927325154 1104 34-35 0.4552943447004978 1104 36-37 0.4981164536926599 1104 38-39 0.566542823482763 1104 40-41 0.4637563972415677 1104 42-43 0.5607986620619272 1104 44-45 0.5138742021632723 1104 46-47 0.45849630367590777 1104 48-49 0.49452034906760645 1104 50-51 0.46556578387566105 1104 52-53 0.4792726129504956 1104 54-55 0.5502346912588436 1104 56-57 0.655238793368504 1104 58-59 0.6293633025965519 1104 60-61 0.6063198020871781 1104 62-63 0.6802262352958834 1104 64-65 0.5790443218999215 1104 66-67 0.5371205411262778 1104 68-69 0.546711169560659 1104 70-71 0.40136070058069606 1104 72-73 0.44688726064796924 1104 74-75 0.5323874232626196 1104 76-77 0.5867099102750828 1104 78-79 0.6624284847288777 1104 80-81 0.5531356243459165 1104 82-83 0.553076316627731 1104 84-85 0.5332852145282843 1104 86-87 0.5916870160552747 1104 88-89 0.6511306160974364 1104 90-91 0.45263783006929614 1104 92-93 0.2716658403655785 1104 94-95 0.3626599601702267 1104 96-97 0.3933862283699945 1104 98-99 0.36128822506549696 1104 100-101 0.2832319270656747 1105 1 0.7043060210769347 1105 2 2.417394603661471 1105 3 1.2426458344366118 1105 4 0.6847195978543397 1105 5 0.41976240962323175 1105 6 0.34386920389004416 1105 7 0.25310965401893526 1105 8 0.17817336845497778 1105 9 0.08622375253735726 1105 10-11 0.12481532630427239 1105 12-13 0.11405228655230815 1105 14-15 0.03120261116898604 1105 16-17 0.04909446617414659 1105 18-19 0.12732281567454606 1105 20-21 0.10782184170002296 1105 22-23 0.13799780319794053 1105 24-25 0.11553374382820891 1105 26-27 0.14328406088277745 1105 28-29 0.140408363190204 1105 30-31 0.21138262572006283 1105 32-33 0.2125878078500918 1105 34-35 0.1506794393805606 1105 36-37 0.2611866345327414 1105 38-39 0.10691132087463018 1105 40-41 0.12284336681591412 1105 42-43 0.19697146284703138 1105 44-45 0.0699800993554831 1105 46-47 0.013199182447856117 1105 48-49 0.007145746087338978 1105 50-51 -0.05935038427477224 1105 52-53 0.015615826511428565 1105 54-55 0.11881084401085928 1105 56-57 0.08287146300017412 1105 58-59 0.0754618831076499 1105 60-61 0.09313898365137874 1105 62-63 0.11235668818843436 1105 64-65 0.24932950697873935 1105 66-67 0.06079596732460146 1105 68-69 -0.005832260005149692 1105 70-71 -0.08632088762656309 1105 72-73 0.008899145892812044 1105 74-75 0.032287952854112234 1105 76-77 0.0074294464731607945 1105 78-79 -0.05406763025914785 1105 80-81 -0.0067178909377183516 1105 82-83 0.03695932239923749 1105 84-85 0.03983026547973978 1105 86-87 -0.004213520011681737 1105 88-89 -0.14262004006849693 1105 90-91 -0.30276776306963527 1105 92-93 -0.3361884725999822 1105 94-95 -0.5288034042307679 1105 96-97 -0.5807930763048681 1105 98-99 -0.5690619833217134 1105 100-101 -0.39313063615784216 1106 1 0.7700667365293974 1106 2 2.593861682577078 1106 3 1.3721547718953495 1106 4 0.8079025208406705 1106 5 0.48045426165418803 1106 6 0.3475776680195821 1106 7 0.2968935694154453 1106 8 0.21493307286934282 1106 9 0.18096865079167657 1106 10-11 0.2017630500311398 1106 12-13 0.21649657033643166 1106 14-15 0.2265249107498093 1106 16-17 0.16425569616161795 1106 18-19 0.18506896675599904 1106 20-21 0.1706783132641263 1106 22-23 0.2383127892156054 1106 24-25 0.22162169134607979 1106 26-27 0.13528574043424868 1106 28-29 0.20683905279788917 1106 30-31 0.21167834691311782 1106 32-33 0.27499879860490495 1106 34-35 0.21233467872044542 1106 36-37 0.20646442401302068 1106 38-39 0.2593846070721497 1106 40-41 0.3276619335959339 1106 42-43 0.2237219310138272 1106 44-45 0.26735968003206523 1106 46-47 0.2806849615656901 1106 48-49 0.054336181478802814 1106 50-51 0.08002375609012802 1106 52-53 0.20600607973722873 1106 54-55 0.3917403530418042 1106 56-57 0.39490649167507996 1106 58-59 0.3283766300358124 1106 60-61 0.42895593287562406 1106 62-63 0.3858327643088515 1106 64-65 0.24756815158583834 1106 66-67 0.2290002653846912 1106 68-69 0.2945753339000632 1106 70-71 0.23513862530234064 1106 72-73 0.34883506887745597 1106 74-75 0.2481980218247486 1106 76-77 0.300267927600963 1106 78-79 0.32952821674249577 1106 80-81 0.28761695929361153 1106 82-83 0.3350967697065528 1106 84-85 0.1756687817247986 1106 86-87 0.22656485077314414 1106 88-89 0.24344497079711047 1106 90-91 0.08070575784672229 1106 92-93 0.07298325505160719 1106 94-95 0.21037739839779945 1106 96-97 0.11468666661811255 1106 98-99 0.050014353355901875 1106 100-101 -0.016426477777962134 1107 1 0.8226672148944978 1107 2 2.588076647612226 1107 3 1.344251781509616 1107 4 0.7372816593988034 1107 5 0.44424015860663246 1107 6 0.2830930444558746 1107 7 0.19152464364344723 1107 8 0.10361237322349126 1107 9 0.08379419872637328 1107 10-11 0.1651040303172593 1107 12-13 0.2092794091787482 1107 14-15 0.23067459599237594 1107 16-17 0.18738051527618182 1107 18-19 0.1880791855809676 1107 20-21 0.1897707291152173 1107 22-23 0.24538789799289162 1107 24-25 0.19359904258836025 1107 26-27 0.2687319440431466 1107 28-29 0.2553763466220431 1107 30-31 0.28914168224597603 1107 32-33 0.21586293222684816 1107 34-35 0.16164487616347145 1107 36-37 0.18950284978301113 1107 38-39 0.17061129102329886 1107 40-41 0.24090508477567596 1107 42-43 0.3299216437442709 1107 44-45 0.1844039415520342 1107 46-47 0.21832743330953264 1107 48-49 0.13749710051772723 1107 50-51 0.05675803085527065 1107 52-53 0.13162045278775025 1107 54-55 0.21680462188889038 1107 56-57 0.3287076501023165 1107 58-59 0.29939137440750585 1107 60-61 0.29339504711737874 1107 62-63 0.3663584760972398 1107 64-65 0.24853934142843315 1107 66-67 0.2837465706249205 1107 68-69 0.17403053939710134 1107 70-71 0.20585453127725373 1107 72-73 0.26865934664412805 1107 74-75 0.3359569256377597 1107 76-77 0.40860773590602406 1107 78-79 0.433323411262279 1107 80-81 0.34794770398022123 1107 82-83 0.44023269714392654 1107 84-85 0.4295116886225365 1107 86-87 0.3477669275951598 1107 88-89 0.2671815573172722 1107 90-91 0.2461218547382451 1107 92-93 0.19411096475458223 1107 94-95 0.15780484963869057 1107 96-97 0.2651557553938879 1107 98-99 0.4583550059793744 1107 100-101 0.49000121770020044 1108 1 0.8168354298694389 1108 2 2.5773437006690045 1108 3 1.3543232890440144 1108 4 0.7782596723644346 1108 5 0.4903382103803722 1108 6 0.37438029633022296 1108 7 0.2989146714812918 1108 8 0.217154469241855 1108 9 0.2744293116102057 1108 10-11 0.22765742845759718 1108 12-13 0.21082087424828444 1108 14-15 0.24603391976741307 1108 16-17 0.26845342421452045 1108 18-19 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-0.8862472633989071 2316 54-55 -1.2791376409521362 2316 56-57 -1.2044503112435265 2316 58-59 -1.1041771969435814 2316 60-61 -1.1950679168500002 2316 62-63 -1.043078050995021 2316 64-65 -1.1517082359069946 2316 66-67 -0.844663126361219 2316 68-69 -0.9491643269043593 2316 70-71 -0.8339149087979578 2316 72-73 -0.87926500384674 2316 74-75 -1.0073117053759617 2316 76-77 -1.0529929176710269 2316 78-79 -0.9966374012343309 2316 80-81 -1.1151857595261383 2316 82-83 -1.4479066422279914 2316 84-85 -1.054079391813545 2316 86-87 -1.1003327948831867 2316 88-89 -1.1419018528543035 2316 90-91 -1.0105218282542872 2316 92-93 -0.7529791258241438 2316 94-95 -0.7438789248365474 2316 96-97 -0.911142810894912 2316 98-99 -1.011797856923124 2316 100-101 -1.3403597186415759 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 38770.0 3 9466.0 4 7990.0 5 6339.0 6 5806.0 7 5654.0 8 6116.0 9 6831.0 10 7708.0 11 8954.0 12 9800.0 13 10817.0 14 11269.0 15 12548.0 16 14388.0 17 16553.0 18 15742.0 19 14182.0 20 15089.0 21 16332.0 22 16970.0 23 19118.0 24 21494.0 25 25048.0 26 29276.0 27 34704.0 28 41671.0 29 51098.0 30 61891.0 31 77962.0 32 99738.0 33 134048.0 34 192821.0 35 303327.0 36 568206.0 37 1101819.0 38 1078630.0 39 137870.0 40 8.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.428205192995115 17.99955084188808 13.547973604994487 25.024270360122316 2 17.592469516887867 20.76402569184345 36.70802673230822 24.935478058960467 3 18.365326355270124 24.15649071803307 29.00125289670379 28.476930029993014 4 13.544014700611061 16.740079991952634 35.843861262274714 33.872044045161594 5 15.393457871318095 35.692107228702 33.634630592856944 15.27980430712296 6 33.18734681745067 34.49062471447044 18.14708405116595 14.174944416912934 7 29.747953088376853 30.593823364341887 21.433915986251687 18.22430756102957 8 27.23490573410802 33.61637381555209 20.287089089750403 18.8616313605895 9 26.94431091975896 16.30722818177783 19.529212945168418 37.2192479532948 10-11 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25.532373089091564 25.501510709918545 24.258420358591565 36-37 24.643806630902702 25.38517809609423 25.61096380651756 24.36005146648551 38-39 24.41041746422935 25.50585689898657 25.848279112056126 24.235446524727955 40-41 24.551956411259834 25.28355877910446 25.887891564096073 24.276593245539633 42-43 24.63663601414158 25.33652595875266 25.642716742596583 24.384121284509174 44-45 24.53042087870418 25.514286888098596 25.51658897801847 24.43870325517876 46-47 24.56723766256809 25.531906605002085 25.45662613893136 24.444229593498466 48-49 24.663476027532692 25.51273786514582 25.458530918558665 24.365255188762823 50-51 24.42807222352485 25.568421298700194 25.527783936742672 24.475722541032283 52-53 24.717568831843415 25.45691830488766 25.369299920609855 24.456212942659068 54-55 24.567425683302087 25.329743879549614 25.47818425802418 24.624646179124117 56-57 24.459876361022793 25.67234773804706 25.605291230661635 24.262484670268513 58-59 24.626646377464866 25.314399788339976 25.578502576153276 24.48045125804188 60-61 24.65444509518808 25.3478746792897 25.68436593598296 24.313314289539257 62-63 24.6103676536912 25.46084810029143 25.570151059570467 24.358633186446905 64-65 24.470506289512365 25.32247991547084 25.539162618278837 24.667851176737955 66-67 24.36993370767748 25.610499107819024 25.46560159795722 24.553965586546276 68-69 24.257547709618397 25.388657921177266 25.732978659480636 24.6208157097237 70-71 24.273521366060326 25.414852378616043 25.64835071948986 24.663275535833773 72-73 24.49148223220449 25.35852992195248 25.48828838656429 24.661699459278733 74-75 24.36762259816779 25.46857157160088 25.394850683323213 24.768955146908116 76-77 24.42196726289495 25.340134889850347 25.57341645629728 24.664481390957427 78-79 24.37416161540316 25.435074528715994 25.566028402303708 24.624735453577134 80-81 24.305297471280436 25.57587882319552 25.58182886269717 24.53699484282687 82-83 24.448871487657698 25.43662156395041 25.49053704756356 24.623969900828328 84-85 24.350134204228425 25.346619488985056 25.47168630776873 24.831559999017788 86-87 24.435285765844814 25.25791398022873 25.700868542292366 24.60593171163409 88-89 24.334174525169345 25.213512197281624 25.63995218488511 24.81236109266392 90-91 24.284618556691296 25.39545538418929 25.73484298719174 24.58508307192767 92-93 24.36477195317547 25.18552490674899 25.772242590428007 24.677460549647538 94-95 24.346273906830753 25.155680282709987 25.692658712279265 24.80538709818 96-97 24.509558070429353 25.150912705157708 25.623367954616516 24.716161269796423 98-99 24.177935795086338 25.143707522971585 25.631844136489722 25.046512545452355 100-101 24.11172519202989 24.97960697508779 25.94240816051726 24.96625967236506 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 178.0 1 130.0 2 153.5 3 215.0 4 164.5 5 143.5 6 208.0 7 380.5 8 634.5 9 820.5 10 814.5 11 816.0 12 938.0 13 1023.0 14 1047.5 15 1088.5 16 1153.5 17 1167.0 18 1219.5 19 1341.0 20 1444.5 21 1659.0 22 2020.5 23 2519.0 24 3156.0 25 4076.0 26 5687.5 27 7867.0 28 10398.0 29 14055.0 30 18859.5 31 24189.5 32 29758.5 33 35898.5 34 42991.0 35 49722.0 36 57600.5 37 67198.0 38 77328.5 39 84866.5 40 91524.5 41 104506.0 42 117720.5 43 129598.0 44 142246.5 45 153069.5 46 161750.0 47 170586.5 48 184176.5 49 194084.5 50 198171.5 51 200819.0 52 199111.5 53 197823.0 54 195653.0 55 185055.0 56 170305.5 57 156376.0 58 141055.5 59 122846.0 60 103246.5 61 83283.5 62 66048.0 63 51960.0 64 40291.0 65 30418.0 66 22445.5 67 17725.5 68 13433.5 69 9616.5 70 7058.0 71 5037.5 72 3718.0 73 2843.0 74 2171.0 75 1662.0 76 1157.0 77 750.0 78 458.5 79 297.5 80 199.0 81 145.0 82 129.0 83 105.0 84 75.5 85 56.0 86 51.0 87 48.0 88 43.5 89 41.5 90 34.5 91 27.5 92 27.5 93 26.0 94 25.5 95 21.0 96 15.5 97 12.5 98 11.0 99 9.5 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03456047410171686 2 1.0768987073580052 3 0.025188542258560032 4 0.026604955131581214 5 0.029603029046142718 6 0.011850654370943896 7 0.018177298537105178 8 0.020113062796900796 9 0.010977199765914167 10-11 0.01072932751313546 12-13 0.024562959906309013 14-15 0.014270359695688415 16-17 0.02608560374480678 18-19 0.030228611398393737 20-21 0.04305895133984396 22-23 0.030323038923261818 24-25 0.00904143550611855 26-27 0.015132010860109636 28-29 0.02450394270326646 30-31 0.018720256805096633 32-33 0.03019320107656821 34-35 0.026522331047321646 36-37 0.025117721614908974 38-39 0.011626388999382209 40-41 0.01311362251605445 42-43 0.010823755038003537 44-45 0.018200905418322196 46-47 0.01318444315970551 48-49 0.010233583007578045 50-51 0.024409515178398383 52-53 0.015120207419501123 54-55 0.01611169643061595 56-57 0.019334035716739143 58-59 0.022650802527730413 60-61 0.017174006085381843 62-63 0.0244331220596154 64-65 0.01954649764769232 66-67 0.015238241825586224 68-69 0.014459214745424573 70-71 0.016312354920960618 72-73 0.020691431386717776 74-75 0.027407589092959886 76-77 0.02459837022813454 78-79 0.022993102305377197 80-81 0.018578615517794512 82-83 0.0170087579168627 84-85 0.016926133832603135 86-87 0.022863264458683592 88-89 0.023382615845458022 90-91 0.018354350146232823 92-93 0.038915943686257 94-95 0.05799030370960892 96-97 0.0722252530834718 98-99 0.07846927316537353 100-101 0.0705373610764549 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4236053.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.15302363734725 #Duplication Level Percentage of deduplicated Percentage of total 1 77.1711341427257 40.24707983066469 2 11.57991152212486 12.07854798663535 3 4.073574586732629 6.373476951310917 4 2.0181851644117006 4.210178343364278 5 1.2297429933481663 3.206740769997454 6 0.7972482471659154 2.4947344007566588 7 0.55266202743528 2.017609704620749 8 0.415958524328561 1.7354795801170817 9 0.306248771119502 1.437461945919354 >10 1.6934965101944932 15.861741360732731 >50 0.10657969998280087 3.8270650635169554 >100 0.05180824568841565 4.942909562838213 >500 0.0025296809113558227 0.8903629912291788 >1k 9.198838308672892E-4 0.6766115082962808 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.360688121701971E-5 2 0.0 0.0 0.0 2.360688121701971E-5 2.360688121701971E-5 3 0.0 0.0 0.0 2.360688121701971E-5 2.360688121701971E-5 4 0.0 0.0 0.0 2.360688121701971E-5 2.360688121701971E-5 5 0.0 0.0 0.0 2.360688121701971E-5 2.360688121701971E-5 6 0.0 0.0 0.0 2.360688121701971E-5 4.721376243403942E-5 7 0.0 0.0 0.0 4.721376243403942E-5 4.721376243403942E-5 8 0.0 0.0 0.0 4.721376243403942E-5 4.721376243403942E-5 9 0.0 0.0 0.0 7.082064365105913E-5 4.721376243403942E-5 10-11 0.0 0.0 0.0 7.082064365105913E-5 4.721376243403942E-5 12-13 2.360688121701971E-5 0.0 0.0 9.442752486807885E-5 4.721376243403942E-5 14-15 2.360688121701971E-5 0.0 0.0 9.442752486807885E-5 5.901720304254928E-5 16-17 2.360688121701971E-5 0.0 0.0 9.442752486807885E-5 7.082064365105913E-5 18-19 2.360688121701971E-5 0.0 0.0 1.1803440608509856E-4 1.062309654765887E-4 20-21 2.360688121701971E-5 0.0 0.0 1.298378466936084E-4 1.4164128730211826E-4 22-23 2.360688121701971E-5 0.0 0.0 1.888550497361577E-4 1.4164128730211826E-4 24-25 2.360688121701971E-5 0.0 0.0 2.3606881217019712E-4 1.65248168519138E-4 26-27 2.360688121701971E-5 0.0 0.0 3.895135400808252E-4 2.2426537156168726E-4 28-29 2.360688121701971E-5 0.0 0.0 8.498477238127096E-4 2.47872252778707E-4 30-31 2.360688121701971E-5 1.1803440608509856E-5 0.0 0.0025731500526551486 2.5967569338721685E-4 32-33 2.360688121701971E-5 2.360688121701971E-5 0.0 0.006137789116425125 2.5967569338721685E-4 34-35 5.901720304254928E-5 2.360688121701971E-5 0.0 0.010339813973054633 2.832825746042365E-4 36-37 9.442752486807885E-5 2.360688121701971E-5 0.0 0.017964836606152 2.832825746042365E-4 38-39 9.442752486807885E-5 2.360688121701971E-5 0.0 0.031007638478555394 2.832825746042365E-4 40-41 9.442752486807885E-5 2.360688121701971E-5 0.0 0.04663539384422244 2.832825746042365E-4 42-43 1.062309654765887E-4 4.721376243403942E-5 0.0 0.06369136552351917 2.832825746042365E-4 44-45 1.4164128730211826E-4 4.721376243403942E-5 0.0 0.08288375995295622 3.0688945582125625E-4 46-47 1.65248168519138E-4 4.721376243403942E-5 0.0 0.10509783517817176 3.30496337038276E-4 48-49 1.65248168519138E-4 4.721376243403942E-5 0.0 0.1286456991921489 3.30496337038276E-4 50-51 1.65248168519138E-4 4.721376243403942E-5 0.0 0.15468408917452164 3.30496337038276E-4 52-53 1.65248168519138E-4 5.901720304254928E-5 0.0 0.18200905418322197 3.4229977764678584E-4 54-55 1.65248168519138E-4 7.082064365105913E-5 0.0 0.21300488922116884 3.5410321825529565E-4 56-57 2.124619309531774E-4 7.082064365105913E-5 0.0 0.24889915211164731 3.5410321825529565E-4 58-59 2.124619309531774E-4 7.082064365105913E-5 0.0 0.2894675774830957 3.5410321825529565E-4 60-61 2.124619309531774E-4 7.082064365105913E-5 0.0 0.33645707454557344 3.659066588638055E-4 62-63 2.124619309531774E-4 7.082064365105913E-5 0.0 0.38532331866480424 3.777100994723154E-4 64-65 2.124619309531774E-4 7.082064365105913E-5 0.0 0.4402565312568091 3.777100994723154E-4 66-67 2.124619309531774E-4 7.082064365105913E-5 0.0 0.5045380688107538 3.777100994723154E-4 68-69 2.124619309531774E-4 7.082064365105913E-5 0.0 0.5783095726139404 3.777100994723154E-4 70-71 2.124619309531774E-4 7.082064365105913E-5 0.0 0.6613939910572413 4.131204212978449E-4 72-73 2.124619309531774E-4 7.082064365105913E-5 0.0 0.7500142231459332 4.3672730251486465E-4 74-75 2.124619309531774E-4 7.082064365105913E-5 0.0 0.8511224953984287 4.603341837318844E-4 76-77 2.124619309531774E-4 7.082064365105913E-5 0.0 0.9681063952693698 4.7213762434039424E-4 78-79 2.124619309531774E-4 7.082064365105913E-5 0.0 1.0975075146604634 4.7213762434039424E-4 80-81 2.124619309531774E-4 7.082064365105913E-5 0.0 1.2444131364739772 4.7213762434039424E-4 82-83 2.124619309531774E-4 7.082064365105913E-5 0.0 1.4071471721435025 5.193513867744337E-4 84-85 2.124619309531774E-4 8.2624084259569E-5 0.0 1.5854617494162608 5.193513867744337E-4 86-87 2.2426537156168726E-4 9.442752486807885E-5 0.0 1.7789437478709544 5.311548273829435E-4 88-89 2.3606881217019712E-4 9.442752486807885E-5 0.0 1.9929637329844552 5.783685898169829E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4800 0.0 37.594234 1 ATCAACG 5565 0.0 33.623123 3 GGTATCA 3250 0.0 33.225677 1 TCAACGC 5665 0.0 33.112648 4 CAACGCA 5890 0.0 31.68461 5 AACGCAG 6320 0.0 29.905245 6 TATCAAC 6165 0.0 28.881218 2 ACGCAGA 7250 0.0 25.742838 7 GTACATG 9655 0.0 25.40056 1 CGCAGAG 7670 0.0 24.396835 8 ACATGGG 10570 0.0 23.76773 3 TACATGG 10280 0.0 23.155983 2 GTACCTG 3910 0.0 20.989109 1 TATAACG 255 1.0604708E-8 20.70271 2 CATGGGG 9145 0.0 19.73316 4 TATCACG 320 3.45608E-10 19.497011 2 TACCTGG 4260 0.0 18.814018 2 GCAGAGT 10210 0.0 18.1858 9 AGAGTAC 8970 0.0 17.365236 10-11 ATGGGGA 6290 0.0 17.21295 5 >>END_MODULE