FastQCFastQC Report
Fri 27 May 2016
SRR940218_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940218_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4236053
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49300.038.935481
GGTATCA33150.033.5384831
ATCAACG58550.031.562193
TCAACGC59400.031.5104224
CAACGCA62050.030.161845
AACGCAG65850.028.5655676
TATCAAC66400.028.3326322
GTACATG103300.024.2393741
ACGCAGA79800.023.5716847
TACATGG108400.022.7018012
GTACCTG40300.022.5184971
ACATGGG107600.022.1633743
CGCAGAG87100.021.6498688
TATAACG2005.3852455E-721.378262
TACCTGG41950.020.8375032
TATCACG3503.6379788E-1220.3602472
TATAAGC21400.019.9789733
CATGGGG95200.019.0621054
ATAGTAC8650.018.1235083
CCTATAA23150.017.8558031
Produced by FastQC (version 0.11.5)