FastQCFastQC Report
Fri 27 May 2016
SRR940217_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940217_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3961063
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49350.036.6721531
ATCAACG53750.033.3141373
TCAACGC55500.032.434854
CAACGCA56500.031.7767145
TATAACG2250.031.6646272
GGTATCA31100.031.3106461
AACGCAG61500.029.57946
TATCAAC63000.028.7243422
ACGCAGA71850.025.51687
GTACATG94750.024.7654551
TATCACG2950.024.150992
CGCAGAG77350.023.51858
GTACCTG39950.022.8287961
GTATAAC7750.022.677681
TACATGG101000.022.6198312
ACATGGG103000.021.5350233
TACCTGG43050.020.080012
CATGGGG87500.019.9758594
GCAGAGT96200.018.8607719
ATAACGC3951.8189894E-1118.0368143
Produced by FastQC (version 0.11.5)