##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940217_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3961063 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.151595670152176 34.0 31.0 34.0 30.0 34.0 2 31.76980497406883 34.0 31.0 34.0 30.0 34.0 3 32.17656901695328 34.0 31.0 34.0 30.0 34.0 4 35.67714297904376 37.0 35.0 37.0 35.0 37.0 5 35.746091137656734 37.0 35.0 37.0 35.0 37.0 6 35.82089530007475 37.0 37.0 37.0 35.0 37.0 7 35.80368325371245 37.0 37.0 37.0 35.0 37.0 8 35.80442446888625 37.0 37.0 37.0 35.0 37.0 9 37.55952707644387 39.0 39.0 39.0 35.0 39.0 10-11 37.50206712188117 39.0 38.0 39.0 35.0 39.0 12-13 37.421398624561135 39.0 38.0 39.0 35.0 39.0 14-15 38.86346619581663 41.0 39.0 41.0 36.0 41.0 16-17 38.793494069647465 41.0 39.0 41.0 36.0 41.0 18-19 38.70954173160084 41.0 39.0 41.0 35.0 41.0 20-21 38.63183746383231 41.0 39.0 41.0 35.0 41.0 22-23 38.52456335584665 40.0 39.0 41.0 34.5 41.0 24-25 38.42164199357597 40.0 38.5 41.0 34.0 41.0 26-27 38.26111778580649 40.0 38.0 41.0 34.0 41.0 28-29 38.10148816113251 40.0 38.0 41.0 34.0 41.0 30-31 37.95502242706061 40.0 38.0 41.0 33.5 41.0 32-33 38.032894452827435 40.0 38.0 41.0 34.0 41.0 34-35 38.105978369947664 40.0 38.0 41.0 33.5 41.0 36-37 38.23601429717225 40.5 38.0 41.0 34.0 41.0 38-39 38.162381537481224 40.5 38.0 41.0 34.0 41.0 40-41 38.03660595653237 40.0 38.0 41.0 33.0 41.0 42-43 37.90717352892393 40.0 38.0 41.0 33.0 41.0 44-45 37.77219183335382 40.0 38.0 41.0 33.0 41.0 46-47 37.57644488360826 40.0 38.0 41.0 33.0 41.0 48-49 37.40245888540525 40.0 38.0 41.0 32.5 41.0 50-51 36.489405874130256 39.0 36.0 40.5 31.0 40.5 52-53 36.48813209989339 39.0 36.5 40.5 31.0 41.0 54-55 36.8780752792874 40.0 36.5 41.0 31.0 41.0 56-57 36.76173201486571 40.0 36.0 41.0 31.0 41.0 58-59 36.50726976571693 39.0 35.5 41.0 31.0 41.0 60-61 36.204084358163456 39.0 35.0 41.0 31.0 41.0 62-63 35.864318492283516 38.5 35.0 40.5 30.0 41.0 64-65 35.48589545281153 37.5 35.0 40.0 29.5 41.0 66-67 35.088875511447306 37.0 35.0 40.0 29.0 41.0 68-69 34.65943005703267 36.5 35.0 39.0 29.0 41.0 70-71 34.24374063224947 36.0 34.0 39.0 28.5 40.5 72-73 33.827277551505745 35.5 34.0 38.5 28.0 40.0 74-75 33.408109515046846 35.0 34.0 37.0 27.5 39.0 76-77 32.912703231430555 35.0 33.5 37.0 26.5 39.0 78-79 32.5063804842286 35.0 33.0 36.0 26.0 38.5 80-81 32.174004301370616 35.0 33.0 36.0 25.5 37.0 82-83 31.839449915338385 35.0 33.0 35.0 25.0 37.0 84-85 31.566386093833906 35.0 33.0 35.0 24.5 36.5 86-87 31.289496784070337 35.0 32.0 35.0 23.5 36.0 88-89 31.016424505240135 35.0 32.0 35.0 22.0 36.0 90-91 30.741911577775966 34.5 32.0 35.0 20.0 35.0 92-93 30.473160361246464 34.0 31.5 35.0 18.5 35.0 94-95 30.147116443237586 34.0 31.0 35.0 13.5 35.0 96-97 29.787200178335965 34.0 31.0 35.0 3.5 35.0 98-99 29.374005664641032 34.0 31.0 35.0 2.0 35.0 100-101 27.51945651962617 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.1361165528203685 1101 2 0.1438679176764417 1101 3 0.11845243468718536 1101 4 0.12454812595233022 1101 5 0.2173402798059385 1101 6 0.3100454100770804 1101 7 0.24132053229656947 1101 8 0.31858926499777596 1101 9 0.4399380603147378 1101 10-11 0.5204812592331223 1101 12-13 0.5216841174979763 1101 14-15 0.5615582982673999 1101 16-17 0.6294312408917548 1101 18-19 0.649849599009201 1101 20-21 0.6699772795916985 1101 22-23 0.6418450941411749 1101 24-25 0.5931664251660536 1101 26-27 0.7405498652422011 1101 28-29 0.6821775439370654 1101 30-31 0.7467990524757511 1101 32-33 0.7100664118412396 1101 34-35 0.6634654190398095 1101 36-37 0.7006768825372731 1101 38-39 0.7703606948002033 1101 40-41 0.7309415421376357 1101 42-43 0.7969772046921619 1101 44-45 0.7511592135229463 1101 46-47 0.7010191810033604 1101 48-49 0.7585002482330907 1101 50-51 0.7125424170665156 1101 52-53 0.8314703968143391 1101 54-55 0.8435984032754149 1101 56-57 0.8851602868067587 1101 58-59 0.9182518606349106 1101 60-61 0.9868160986824464 1101 62-63 0.8929804725352497 1101 64-65 0.9366704492125564 1101 66-67 0.9262689568305262 1101 68-69 0.8755407192622187 1101 70-71 0.7774034358395454 1101 72-73 0.8813308382095855 1101 74-75 0.7897183158034764 1101 76-77 0.9299235595792297 1101 78-79 0.9441669454604877 1101 80-81 0.8565337416140011 1101 82-83 0.769871394095329 1101 84-85 0.8569518135281662 1101 86-87 0.8926234338896002 1101 88-89 0.9075750525412047 1101 90-91 0.9316511363487194 1101 92-93 0.8957782001644432 1101 94-95 0.8716900783934953 1101 96-97 0.8414610326889687 1101 98-99 0.911768398962927 1101 100-101 1.1099722844559778 1102 1 0.1832726144553689 1102 2 0.6881095903712726 1102 3 0.3008682469757886 1102 4 0.23166984066343588 1102 5 0.19008323901320523 1102 6 0.18416999544042056 1102 7 0.14710286941908635 1102 8 0.19770745681716306 1102 9 0.20037570683723516 1102 10-11 0.12802514202891047 1102 12-13 0.17860106821124333 1102 14-15 0.2043360889504342 1102 16-17 0.20554830535674995 1102 18-19 0.21038895605423846 1102 20-21 0.2729230918113288 1102 22-23 0.2301296734503424 1102 24-25 0.28224831915045456 1102 26-27 0.20827199597827928 1102 28-29 0.18468233228952613 1102 30-31 0.22605696582481016 1102 32-33 0.1670370715903715 1102 34-35 0.2263256797250932 1102 36-37 0.2833697423478938 1102 38-39 0.27182773456576115 1102 40-41 0.2749349046844287 1102 42-43 0.11506622467421579 1102 44-45 0.21164546041645593 1102 46-47 0.31888392787673325 1102 48-49 0.25772283285090936 1102 50-51 0.24759180506116962 1102 52-53 0.377699775684178 1102 54-55 0.39035643367913764 1102 56-57 0.3893461707037815 1102 58-59 0.4019095321798787 1102 60-61 0.3311821215424047 1102 62-63 0.4289577682857413 1102 64-65 0.39952859565465104 1102 66-67 0.2768715887275377 1102 68-69 0.32840906670079306 1102 70-71 0.3081046059869408 1102 72-73 0.2583662282810977 1102 74-75 0.2713778460676437 1102 76-77 0.38903164658659506 1102 78-79 0.43592538963884664 1102 80-81 0.33377423142152907 1102 82-83 0.2100566870931324 1102 84-85 0.10589528175081497 1102 86-87 0.12340065327327565 1102 88-89 0.08497693260245853 1102 90-91 0.16213812368104286 1102 92-93 0.18940324884647808 1102 94-95 0.06322004819883631 1102 96-97 0.0637248844298881 1102 98-99 -0.014041174693133485 1102 100-101 0.3710691120749239 1103 1 0.06744328395497945 1103 2 0.5557828310257129 1103 3 0.15601403264987823 1103 4 0.10024982888847944 1103 5 0.025419075067595998 1103 6 0.01244140163508689 1103 7 0.03551942040007816 1103 8 -0.028552539402681987 1103 9 0.01173446494880892 1103 10-11 -0.04474584487307709 1103 12-13 0.013684215252631304 1103 14-15 0.03468861524312672 1103 16-17 -0.041842113696951344 1103 18-19 -0.010314190161594183 1103 20-21 -0.03081453308031712 1103 22-23 -0.011511900830726063 1103 24-25 -0.056677982014655015 1103 26-27 0.06249039532306 1103 28-29 -0.1151733050244772 1103 30-31 -0.03634760707237206 1103 32-33 -0.05877987022353892 1103 34-35 -0.13559613631187517 1103 36-37 -0.13895316623979426 1103 38-39 -0.15388916358087812 1103 40-41 -0.1568643171257449 1103 42-43 -0.08967189120264152 1103 44-45 -0.13182000985396058 1103 46-47 -0.1807387358700936 1103 48-49 -0.12472273513859022 1103 50-51 -0.19470847149819548 1103 52-53 -0.060877920552400155 1103 54-55 -0.049171324365083535 1103 56-57 0.056912388531763725 1103 58-59 0.026733831168151312 1103 60-61 0.030827623705128815 1103 62-63 -0.05566825740624637 1103 64-65 0.013207014465038469 1103 66-67 -0.03846949934781918 1103 68-69 -0.04633702225442704 1103 70-71 -0.11666060097635977 1103 72-73 -0.1146198633680271 1103 74-75 -0.024751816390889303 1103 76-77 0.0548439772673035 1103 78-79 0.0788227237629684 1103 80-81 0.013028911531634435 1103 82-83 -0.09954343152541512 1103 84-85 -0.19019256006505003 1103 86-87 -0.09144067812218992 1103 88-89 -0.11880460396399428 1103 90-91 -0.15186266710929175 1103 92-93 -0.2284178304954274 1103 94-95 -0.11525904908357987 1103 96-97 -0.1795493242909636 1103 98-99 -0.37071653679472405 1103 100-101 -0.18940666019483743 1104 1 0.10244206607652018 1104 2 0.6388905800541842 1104 3 0.29984271036602905 1104 4 0.0798226856991846 1104 5 0.07173997496905571 1104 6 0.0807228644178295 1104 7 0.11911016895246718 1104 8 -0.05663570942958529 1104 9 -0.005245251839092191 1104 10-11 0.0021044169707877813 1104 12-13 0.06721758512231446 1104 14-15 -0.028972521148190822 1104 16-17 -0.023949680043649835 1104 18-19 0.08787103254408635 1104 20-21 0.11028498973276157 1104 22-23 0.0018072608260339962 1104 24-25 -0.029539359767333906 1104 26-27 0.027558042935467597 1104 28-29 -0.047378993624036525 1104 30-31 -0.07169722122633715 1104 32-33 -0.07889354810575355 1104 34-35 -0.06716797453333356 1104 36-37 -0.04154018919125946 1104 38-39 -0.04870252370753292 1104 40-41 -0.020391348491649808 1104 42-43 -0.16644113645014613 1104 44-45 -0.07397977889991836 1104 46-47 -0.15243067351471495 1104 48-49 -0.0847313433249468 1104 50-51 -0.10666693077123313 1104 52-53 -0.03299680763292656 1104 54-55 -0.04257609734147394 1104 56-57 -0.0459159957863946 1104 58-59 -0.03327574578516135 1104 60-61 0.012973248753567646 1104 62-63 0.025082620019816204 1104 64-65 0.005511544972897298 1104 66-67 0.03813989935237316 1104 68-69 -0.01000826150112033 1104 70-71 -0.038777273732421236 1104 72-73 -0.13184453979986444 1104 74-75 -0.10727622931811709 1104 76-77 0.03365842794876528 1104 78-79 -0.02950202906306032 1104 80-81 -0.10873125498557101 1104 82-83 -0.22974571006528777 1104 84-85 -0.13767777944924475 1104 86-87 -0.04308675157133379 1104 88-89 -0.04998547552210297 1104 90-91 -0.22289542803974882 1104 92-93 -0.2706782129092602 1104 94-95 -0.34283209411187343 1104 96-97 -0.32200373688604245 1104 98-99 -0.38016409051977007 1104 100-101 -0.0967572486515671 1105 1 -0.01198314232178177 1105 2 0.4956347366390368 1105 3 0.13374121004127204 1105 4 0.1807573971418961 1105 5 0.1160237046914574 1105 6 0.12326403516715345 1105 7 0.09124352854231432 1105 8 0.009569590963572239 1105 9 0.12628749256167282 1105 10-11 0.08487970361503017 1105 12-13 -0.1286476717126206 1105 14-15 -0.234775185060478 1105 16-17 -0.20869113384227234 1105 18-19 -0.26906027832255575 1105 20-21 -0.16685308351422634 1105 22-23 -0.2160730480404709 1105 24-25 -0.11198040584930169 1105 26-27 -0.08824649443523924 1105 28-29 0.0685019443260586 1105 30-31 -0.046934621739559645 1105 32-33 0.05206729342060612 1105 34-35 -0.07254206727246526 1105 36-37 -0.03128868729570655 1105 38-39 -0.2888578636770234 1105 40-41 -0.28996704144778107 1105 42-43 -0.15429484910325897 1105 44-45 -0.2527694264266529 1105 46-47 -0.16275998076419285 1105 48-49 -0.2911616221931297 1105 50-51 -0.3137773759340732 1105 52-53 -0.11305567534887473 1105 54-55 -0.2663107083555687 1105 56-57 -0.10980632350497643 1105 58-59 -0.1796692808594429 1105 60-61 -0.1955147055600932 1105 62-63 -0.16300424051085116 1105 64-65 -0.0830412281925419 1105 66-67 -0.18694351871440773 1105 68-69 -0.17606263802513666 1105 70-71 -0.2756250720292712 1105 72-73 -0.3594218158092062 1105 74-75 -0.38176968677222334 1105 76-77 -0.2775112665178838 1105 78-79 -0.2549485174970485 1105 80-81 -0.24383043462277243 1105 82-83 -0.3049204320923309 1105 84-85 -0.3470919590902817 1105 86-87 -0.276683518444397 1105 88-89 -0.2764269274562068 1105 90-91 -0.40966687683993186 1105 92-93 -0.4368749824897087 1105 94-95 -0.5461041205298045 1105 96-97 -0.5951846879388434 1105 98-99 -0.6698937007918921 1105 100-101 -0.7155050121866289 1106 1 3.3184233441829747E-4 1106 2 0.5701018103166469 1106 3 0.12194938294708635 1106 4 0.0031247473314195418 1106 5 -0.03605337020569266 1106 6 -0.020382039533373586 1106 7 0.08450246166923847 1106 8 -0.031155686606112454 1106 9 -0.005208981973595428 1106 10-11 -0.0910814926287884 1106 12-13 -0.06364478463948586 1106 14-15 -0.04023772653104629 1106 16-17 0.03432376693947248 1106 18-19 0.00536062707352869 1106 20-21 -0.004128806464713364 1106 22-23 0.021262220793332176 1106 24-25 -0.08819092325760636 1106 26-27 -0.2102784090376133 1106 28-29 -0.14652589352994028 1106 30-31 -0.05283221526455151 1106 32-33 -0.06103771639793365 1106 34-35 -0.08263388117570969 1106 36-37 -0.05448837320206934 1106 38-39 -0.023751004638370432 1106 40-41 -0.15654027230660716 1106 42-43 -0.08000230646293005 1106 44-45 -0.0034710971957920833 1106 46-47 -0.08756840106231323 1106 48-49 -0.03946073790254445 1106 50-51 -0.03267886949664245 1106 52-53 0.01784198039751317 1106 54-55 0.006737966204092061 1106 56-57 0.07107807908244013 1106 58-59 0.028866002409721148 1106 60-61 -0.02357170534357067 1106 62-63 -0.08763271650956028 1106 64-65 -0.07438224877289201 1106 66-67 -0.011972638331322116 1106 68-69 -0.25768616866668737 1106 70-71 -0.07482067372268375 1106 72-73 -0.07939183753691736 1106 74-75 0.04836575677928323 1106 76-77 0.041180665357153146 1106 78-79 0.16484243103092666 1106 80-81 0.11804383583127276 1106 82-83 0.07702901426161901 1106 84-85 -0.016056358491962186 1106 86-87 -0.036062150618835886 1106 88-89 -0.041491787047391426 1106 90-91 0.0055185678315510245 1106 92-93 -0.07385988259946785 1106 94-95 -0.0792366446530437 1106 96-97 -0.0570926201722699 1106 98-99 -0.0742778831155384 1106 100-101 -0.14470626358350103 1107 1 0.15665986781596075 1107 2 0.6393319769758783 1107 3 0.27590604841262234 1107 4 0.2089510114963673 1107 5 0.18862494698073107 1107 6 0.1379603825872664 1107 7 0.14414921013298 1107 8 0.05432101376037224 1107 9 0.02146890859218331 1107 10-11 0.0619659526486771 1107 12-13 0.031890341062961625 1107 14-15 0.008981461274842673 1107 16-17 0.05127964785688022 1107 18-19 0.12599460463617618 1107 20-21 0.062339594081457506 1107 22-23 0.037326356097153734 1107 24-25 0.030618347184933725 1107 26-27 0.07841138352111443 1107 28-29 0.04276080639063906 1107 30-31 0.06523408447622359 1107 32-33 0.12201736948658493 1107 34-35 0.11084502942316021 1107 36-37 0.09231896178070542 1107 38-39 0.14471763211584232 1107 40-41 0.15940322817605335 1107 42-43 0.13905064390807098 1107 44-45 0.06164230074124788 1107 46-47 0.02197988721272992 1107 48-49 0.1444699446784412 1107 50-51 -0.05185233417450519 1107 52-53 0.15221573382765285 1107 54-55 0.12029166798179602 1107 56-57 0.10815858384456334 1107 58-59 0.15609030484278463 1107 60-61 0.06882384189382407 1107 62-63 0.10344006569577857 1107 64-65 0.1307440080406863 1107 66-67 0.07097320133403429 1107 68-69 0.2605653615637209 1107 70-71 0.26363013896553866 1107 72-73 0.14094651843101502 1107 74-75 0.2520444559960495 1107 76-77 0.35578103388989746 1107 78-79 0.31443027210147534 1107 80-81 0.2103193508190344 1107 82-83 0.017313632900119558 1107 84-85 -0.07992833855407255 1107 86-87 0.03460683634995121 1107 88-89 0.004341129507299257 1107 90-91 0.10055337838908684 1107 92-93 0.05572204121861901 1107 94-95 0.1601499013707688 1107 96-97 0.08248150637016138 1107 98-99 0.2068733780064278 1107 100-101 0.4198904895200428 1108 1 0.0897878052328096 1108 2 0.28994930837240673 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-1.0085183987906277 2316 70-71 -0.9279337199776521 2316 72-73 -1.1048999236737345 2316 74-75 -1.235886067674052 2316 76-77 -0.9608513465641657 2316 78-79 -0.8624904410610981 2316 80-81 -1.0334277013662891 2316 82-83 -1.1958250494295974 2316 84-85 -0.9797984669413253 2316 86-87 -0.9873590591932704 2316 88-89 -0.9220927090865203 2316 90-91 -0.7981912093755881 2316 92-93 -0.8241213265952361 2316 94-95 -0.8279134586286538 2316 96-97 -0.7406835168653032 2316 98-99 -0.8117681538065362 2316 100-101 -1.240863103370888 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 27735.0 3 8752.0 4 6768.0 5 5353.0 6 4843.0 7 4651.0 8 4825.0 9 5469.0 10 6246.0 11 7246.0 12 7884.0 13 8758.0 14 9458.0 15 10426.0 16 11947.0 17 14199.0 18 13660.0 19 12251.0 20 12824.0 21 13749.0 22 14112.0 23 16029.0 24 17904.0 25 20685.0 26 24272.0 27 28325.0 28 35208.0 29 42720.0 30 52193.0 31 65876.0 32 84788.0 33 114457.0 34 163722.0 35 260772.0 36 497321.0 37 1037096.0 38 1127252.0 39 161281.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.409443422246845 17.97472043330628 13.54789213203033 25.067944012416543 2 16.596832894699293 19.931301852869996 37.90060765609812 25.571257596332597 3 18.369647565077727 24.12469569784943 28.972393102821297 28.533263634251544 4 13.58576719290794 16.699124300447927 35.871383932562196 33.84372457408194 5 15.398546973366228 35.74445519076972 33.60016767634425 15.256830159519799 6 33.22759828662253 34.49550973282645 18.09329432222749 14.18359765832352 7 29.750539029035593 30.5700550872775 21.44941612132434 18.229989762362568 8 27.32510834688985 33.5854651277309 20.255678962900188 18.833747562479072 9 26.968789435577374 16.26198519564592 19.502442180514528 37.26678318826218 10-11 25.69220286462381 25.316553820872446 27.403694955402248 21.587548359101497 12-13 26.34515362370737 22.4301256734717 27.560915958130643 23.66380474469029 14-15 23.74768236628745 23.778211686366156 24.661163055987465 27.812942891358926 16-17 24.331411493107968 26.730672726579357 25.22144433250757 23.716471447805105 18-19 24.38520457484703 26.09274689465721 26.22169497458608 23.300353555909684 20-21 24.71462587761643 25.57467893082813 25.996616768527737 23.714078423027697 22-23 24.627698706116156 25.22981319714549 25.955739792793718 24.18674830394464 24-25 24.537503157755996 25.529136382613597 25.740524554243528 24.192835905386875 26-27 24.42194991873992 25.758852500578268 25.648313032622212 24.170884548059608 28-29 24.42932487187225 25.64720454858248 25.811968186185812 24.111502393359455 30-31 24.464854122290557 25.48225439678585 25.718529947638824 24.334361533284763 32-33 24.315776474438753 25.49899690485041 25.743381390312102 24.44184523039873 34-35 24.73713599073858 25.521283183471677 25.449660727760925 24.291920098028815 36-37 24.616520842729855 25.373453393329658 25.606697614373257 24.403328149567223 38-39 24.435278792076492 25.49703723212056 25.831101399206176 24.23658257659677 40-41 24.542038942691903 25.261631613699 25.893746520268607 24.30258292334049 42-43 24.511548006404833 25.353271494330627 25.697462570814317 24.43771792845022 44-45 24.522495448715205 25.496298052685184 25.496361181678807 24.484845316920804 46-47 24.569129426308862 25.50549774085852 25.45959275357708 24.46578007925554 48-49 24.666692086084964 25.49523045017369 25.433680948087066 24.404396515654277 50-51 24.438231400154592 25.575013124579087 25.52901712495584 24.457738350310482 52-53 24.766892121814216 25.40272639656073 25.30874711846755 24.521634363157496 54-55 24.56894908097775 25.314369121559427 25.508047521222043 24.608634276240778 56-57 24.476271144493452 25.677979927157534 25.587065739543984 24.25868318880503 58-59 24.64730747323421 25.31066450985559 25.584803093342856 24.457224923567345 60-61 24.67733394210307 25.315102733680835 25.66932893995529 24.338234384260808 62-63 24.585315664682536 25.433555864588158 25.55823801916751 24.42289045156179 64-65 24.47028846293468 25.323170535377766 25.535904926111797 24.67063607557576 66-67 24.376634910566178 25.557166915104474 25.492534366107343 24.57366380822201 68-69 24.22387021735321 25.399295673995947 25.756332292786695 24.620501815864156 70-71 24.250205286990774 25.41078548743775 25.647583699827802 24.691425525743675 72-73 24.487074310499853 25.33988625807343 25.504580507523418 24.6684589239033 74-75 24.355207881948548 25.41363260333997 25.422698177491316 24.808461337220166 76-77 24.523673856390406 25.318026440281045 25.512582900550658 24.64571680277789 78-79 24.346130647733595 25.453129930180285 25.577966277393255 24.622773144692864 80-81 24.2969429248841 25.51727551352554 25.609484480787632 24.576297080802732 82-83 24.43699129532059 25.424113379898188 25.495527584430945 24.643367740350275 84-85 24.319591858018992 25.332573062917135 25.46663890568535 24.881196173378523 86-87 24.428110454670907 25.26434575199691 25.693939951755556 24.61360384157663 88-89 24.343334879167962 25.180699145691737 25.628326222199437 24.847639752940864 90-91 24.246829790312525 25.399036469374103 25.760126881615626 24.594006858697746 92-93 24.38599701305912 25.167660272158376 25.732342872122842 24.71399984265966 94-95 24.320019140281513 25.173815660342868 25.657741904066413 24.848423295309203 96-97 24.45299765748197 25.15777443858275 25.601895238834 24.78733266510128 98-99 24.195552638048653 25.12173053303205 25.604079321487617 25.078637507431683 100-101 24.1123815929742 24.97979225503273 25.916423445178584 24.99140270681449 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 306.0 1 197.0 2 159.5 3 203.5 4 144.0 5 138.5 6 207.5 7 377.0 8 625.5 9 778.5 10 790.0 11 790.5 12 838.0 13 895.0 14 964.0 15 1011.0 16 1030.0 17 1071.0 18 1160.5 19 1262.5 20 1375.5 21 1555.0 22 1873.5 23 2371.0 24 2912.0 25 3742.0 26 5154.5 27 7196.5 28 9807.0 29 13168.0 30 17594.5 31 22613.5 32 27655.0 33 33405.0 34 39931.5 35 46076.0 36 53618.0 37 62456.0 38 71800.5 39 79160.0 40 85513.5 41 97742.0 42 110209.0 43 120703.0 44 132675.5 45 142994.5 46 150761.5 47 159413.5 48 172047.5 49 181339.5 50 186130.0 51 188248.5 52 185964.5 53 185098.0 54 183324.5 55 173116.0 56 159143.0 57 146330.0 58 131811.0 59 115050.5 60 97148.0 61 78656.0 62 62043.5 63 48491.0 64 37775.5 65 28714.5 66 21208.5 67 16694.0 68 12881.5 69 9220.0 70 6612.5 71 4760.0 72 3501.0 73 2621.0 74 1989.5 75 1530.5 76 1071.5 77 648.5 78 388.0 79 267.5 80 192.0 81 133.0 82 108.0 83 91.0 84 64.5 85 59.5 86 53.5 87 38.0 88 36.0 89 32.0 90 26.0 91 25.5 92 24.5 93 18.0 94 14.0 95 14.0 96 11.0 97 9.5 98 5.5 99 4.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.029916211885546884 2 0.03589945426265626 3 0.029865720388693638 4 0.025977875130993873 5 0.0265837730932328 6 0.02941129691701445 7 0.02941129691701445 8 0.027694586024004163 9 0.032365049482929203 10-11 0.01811382449610117 12-13 0.02722753967811166 14-15 0.023617397653104735 16-17 0.03761616515566655 18-19 0.043359572922723015 20-21 0.04621234249493129 22-23 0.03620240324377572 24-25 0.014554173967947493 26-27 0.02370575777259791 28-29 0.028982119193761875 30-31 0.026962459319632127 32-33 0.03329914217471421 34-35 0.03562175102996342 36-37 0.03193587175967663 38-39 0.018833328326259894 40-41 0.015854330011918518 42-43 0.015589249653438988 44-45 0.02316297418142554 46-47 0.01789923563447489 48-49 0.02110544568465586 50-51 0.023630020527318045 52-53 0.028969496319548566 54-55 0.028982119193761875 56-57 0.03244078672820907 58-59 0.030408503979866008 60-61 0.029386051168587824 62-63 0.03520519618092416 64-65 0.030181292244026412 66-67 0.024841816451795895 68-69 0.02436214723169008 70-71 0.03439733223127226 72-73 0.01752054940807556 74-75 0.02592738363414063 76-77 0.045467592916345945 78-79 0.02722753967811166 80-81 0.02615459536998023 82-83 0.026924590696992195 84-85 0.026975082193845437 86-87 0.02055003921927018 88-89 0.024198049866917037 90-91 0.02110544568465586 92-93 0.037704525275159724 94-95 0.07367971678309586 96-97 0.08472473171974292 98-99 0.08447227423547668 100-101 0.08639095111589995 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3961063.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.47806845319721 #Duplication Level Percentage of deduplicated Percentage of total 1 77.31670008859403 39.801143797359614 2 11.249003737561395 11.581539688649134 3 4.115565183561235 6.3558403872888105 4 2.0627310132185017 4.247416331959795 5 1.2625629473960283 3.2497150916261583 6 0.7923562563737732 2.4473381762956903 7 0.5561922002701152 2.0042190111047553 8 0.4015538481304637 1.653697318536381 9 0.3151884546563403 1.4602763560010878 >10 1.7542451693786199 16.15574436348126 >50 0.11252454225475703 3.980979113348643 >100 0.05753510310098567 5.368330218218642 >500 0.0028564669613890064 0.9778519079808045 >1k 9.849885423800573E-4 0.7159082381491966 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 5.049149685324369E-5 7 0.0 0.0 0.0 0.0 5.049149685324369E-5 8 0.0 0.0 0.0 0.0 5.049149685324369E-5 9 0.0 0.0 0.0 0.0 5.049149685324369E-5 10-11 0.0 0.0 0.0 0.0 5.049149685324369E-5 12-13 0.0 0.0 0.0 2.5245748426621845E-5 7.573724527986553E-5 14-15 0.0 0.0 0.0 5.049149685324369E-5 1.136058679197983E-4 16-17 0.0 0.0 0.0 5.049149685324369E-5 1.262287421331092E-4 18-19 0.0 0.0 0.0 5.049149685324369E-5 1.5147449055973107E-4 20-21 0.0 0.0 0.0 5.049149685324369E-5 1.64097364773042E-4 22-23 0.0 0.0 0.0 1.262287421331092E-4 1.767202389863529E-4 24-25 2.5245748426621845E-5 0.0 0.0 2.0196598741297476E-4 1.8934311319966383E-4 26-27 2.5245748426621845E-5 0.0 0.0 3.029489811194621E-4 2.0196598741297476E-4 28-29 3.786862263993277E-5 0.0 0.0 6.690123333054788E-4 2.0196598741297476E-4 30-31 5.049149685324369E-5 0.0 0.0 0.0023604774778891425 2.0196598741297476E-4 32-33 6.311437106655462E-5 0.0 0.0 0.005718162018629847 2.3983461005290752E-4 34-35 8.836011949317645E-5 0.0 0.0 0.009934202005875695 2.524574842662184E-4 36-37 1.0098299370648738E-4 1.2622874213310922E-5 0.0 0.017179731804316166 2.777032326928403E-4 38-39 1.0098299370648738E-4 3.786862263993277E-5 0.0 0.029588017156000804 3.0294898111946214E-4 40-41 1.0098299370648738E-4 5.049149685324369E-5 0.0 0.04385186501704214 3.0294898111946214E-4 42-43 1.136058679197983E-4 5.049149685324369E-5 0.0 0.06000914401008012 3.0294898111946214E-4 44-45 1.262287421331092E-4 5.049149685324369E-5 0.0 0.07886771808476664 3.0294898111946214E-4 46-47 1.262287421331092E-4 5.049149685324369E-5 0.0 0.10076840484486109 3.0294898111946214E-4 48-49 1.262287421331092E-4 5.049149685324369E-5 0.0 0.1256228441708703 3.0294898111946214E-4 50-51 1.262287421331092E-4 6.311437106655462E-5 0.0 0.15171432516978395 3.28194729546084E-4 52-53 1.262287421331092E-4 7.573724527986553E-5 0.0 0.18073431298618578 3.28194729546084E-4 54-55 1.262287421331092E-4 7.573724527986553E-5 0.0 0.21107970259498524 3.28194729546084E-4 56-57 1.262287421331092E-4 7.573724527986553E-5 0.0 0.24795111817206644 3.28194729546084E-4 58-59 1.262287421331092E-4 7.573724527986553E-5 0.0 0.2884705443967945 3.28194729546084E-4 60-61 1.262287421331092E-4 7.573724527986553E-5 0.0 0.3363238605394562 3.534404779727058E-4 62-63 1.262287421331092E-4 7.573724527986553E-5 0.0 0.38838059379515044 3.534404779727058E-4 64-65 1.262287421331092E-4 7.573724527986553E-5 0.0 0.4436940285978789 3.534404779727058E-4 66-67 1.262287421331092E-4 7.573724527986553E-5 0.0 0.509875758098268 3.534404779727058E-4 68-69 1.262287421331092E-4 7.573724527986553E-5 0.0 0.5848303851769083 3.534404779727058E-4 70-71 1.262287421331092E-4 7.573724527986553E-5 0.0 0.6671693936703355 3.660633521860167E-4 72-73 1.262287421331092E-4 7.573724527986553E-5 0.0 0.7592027695595854 4.165548490392604E-4 74-75 1.262287421331092E-4 7.573724527986553E-5 0.0 0.861208216077351 4.291777232525713E-4 76-77 1.262287421331092E-4 7.573724527986553E-5 0.0 0.9773512817140247 4.291777232525713E-4 78-79 1.262287421331092E-4 7.573724527986553E-5 0.0 1.1066852509036085 4.291777232525713E-4 80-81 1.5147449055973107E-4 7.573724527986553E-5 0.0 1.2522648592057233 4.544234716791932E-4 82-83 1.5147449055973107E-4 7.573724527986553E-5 0.0 1.4142542039851422 5.049149685324368E-4 84-85 1.5147449055973107E-4 7.573724527986553E-5 0.0 1.5919842729085603 5.049149685324368E-4 86-87 1.5147449055973107E-4 7.573724527986553E-5 0.0 1.7870203023784272 5.049149685324368E-4 88-89 1.5147449055973107E-4 7.573724527986553E-5 0.0 2.001899490111619 5.049149685324368E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4935 0.0 36.672153 1 ATCAACG 5375 0.0 33.314137 3 TCAACGC 5550 0.0 32.43485 4 CAACGCA 5650 0.0 31.776714 5 TATAACG 225 0.0 31.664627 2 GGTATCA 3110 0.0 31.310646 1 AACGCAG 6150 0.0 29.5794 6 TATCAAC 6300 0.0 28.724342 2 ACGCAGA 7185 0.0 25.5168 7 GTACATG 9475 0.0 24.765455 1 TATCACG 295 0.0 24.15099 2 CGCAGAG 7735 0.0 23.5185 8 GTACCTG 3995 0.0 22.828796 1 GTATAAC 775 0.0 22.67768 1 TACATGG 10100 0.0 22.619831 2 ACATGGG 10300 0.0 21.535023 3 TACCTGG 4305 0.0 20.08001 2 CATGGGG 8750 0.0 19.975859 4 GCAGAGT 9620 0.0 18.860771 9 ATAACGC 395 1.8189894E-11 18.036814 3 >>END_MODULE