FastQCFastQC Report
Fri 27 May 2016
SRR940217_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940217_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3961063
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49250.041.194171
ATCAACG59550.032.70363
TCAACGC59800.032.487454
CAACGCA60250.032.2443965
GGTATCA31100.031.4717541
AACGCAG64700.030.5405656
TATCAAC66200.029.7061792
ACGCAGA75450.026.2518167
CGCAGAG79500.024.9742038
GTACATG94900.024.3824231
TACATGG98100.023.1937482
GTACCTG38800.022.7769371
ACATGGG99350.021.8017043
TACCTGG39950.021.6400892
CATGGGG88250.019.3767644
GCAGAGT103800.019.0361239
TATCACG3506.366463E-1119.0004752
GTATATA14350.018.8728331
AGAGTAC90000.018.78817210-11
TATAACG2051.5906524E-518.5370482
Produced by FastQC (version 0.11.5)