FastQCFastQC Report
Fri 27 May 2016
SRR940216_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940216_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007948
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47050.037.4518971
GGTATCA33650.033.310911
ATCAACG53050.031.8738843
TCAACGC53400.031.8428654
TATAACG2550.031.6657642
CAACGCA55300.030.9213625
AACGCAG59900.028.8646726
TATCAAC59900.028.8639532
ACGCAGA69250.024.967427
GTACATG90550.024.1284221
CGCAGAG74650.023.352528
TATCACG3200.022.264992
TACATGG96950.022.242792
ACATGGG98300.021.5019023
TACCTGG39000.021.4352872
GTACCTG40200.021.2669891
TAACGCA3900.020.7040214
ATAACGC3900.019.4861373
CATGGGG87700.019.2806114
CCTATAA19750.018.036561
Produced by FastQC (version 0.11.5)