##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940216_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4007948 Sequences flagged as poor quality 0 Sequence length 101 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.32876948503324 34.0 31.0 34.0 31.0 34.0 2 32.33525434960733 34.0 31.0 34.0 31.0 34.0 3 32.44363000717574 34.0 31.0 34.0 31.0 34.0 4 35.81995624693734 37.0 37.0 37.0 35.0 37.0 5 35.82196051445777 37.0 37.0 37.0 35.0 37.0 6 35.88981643474416 37.0 37.0 37.0 35.0 37.0 7 35.84746109480462 37.0 37.0 37.0 35.0 37.0 8 35.83662961695112 37.0 37.0 37.0 35.0 37.0 9 37.596373505843886 39.0 39.0 39.0 35.0 39.0 10-11 37.525612233492055 39.0 38.0 39.0 35.0 39.0 12-13 37.4658479850537 39.0 38.0 39.0 35.0 39.0 14-15 38.92304303848253 41.0 39.0 41.0 36.0 41.0 16-17 38.83446292217364 41.0 39.0 41.0 36.0 41.0 18-19 38.77734928197671 41.0 39.0 41.0 36.0 41.0 20-21 38.70677326651943 41.0 39.0 41.0 35.0 41.0 22-23 38.59667365943869 40.5 39.0 41.0 35.0 41.0 24-25 38.47831346614277 40.0 38.5 41.0 34.5 41.0 26-27 38.32091920853264 40.0 38.0 41.0 34.0 41.0 28-29 38.162666157345356 40.0 38.0 41.0 34.0 41.0 30-31 38.13134352042492 40.0 38.0 41.0 34.0 41.0 32-33 38.16240530066757 40.0 38.0 41.0 34.0 41.0 34-35 38.189539634745756 40.0 38.0 41.0 34.0 41.0 36-37 38.326395951244876 40.5 39.0 41.0 34.0 41.0 38-39 38.254732222074736 41.0 38.0 41.0 34.0 41.0 40-41 38.14305213041686 40.0 38.0 41.0 34.0 41.0 42-43 38.027680124592436 40.0 38.0 41.0 33.0 41.0 44-45 37.87833437459767 40.0 38.0 41.0 33.0 41.0 46-47 37.71073826307128 40.0 38.0 41.0 33.0 41.0 48-49 37.52807459078811 40.0 38.0 41.0 33.0 41.0 50-51 36.59452205966744 39.0 36.0 40.5 31.0 40.5 52-53 36.67678260795799 39.5 36.5 40.5 31.5 41.0 54-55 36.92816199212165 40.0 37.0 41.0 31.5 41.0 56-57 36.85787128475719 40.0 36.0 41.0 31.5 41.0 58-59 36.62486152514953 39.0 36.0 41.0 31.0 41.0 60-61 36.315940476273646 39.0 35.0 41.0 31.0 41.0 62-63 35.965321905374026 38.5 35.0 41.0 30.5 41.0 64-65 35.57418272392756 37.5 35.0 40.0 30.0 41.0 66-67 35.18131360486713 37.0 35.0 40.0 29.5 41.0 68-69 34.775303472001134 36.5 35.0 39.0 29.0 41.0 70-71 34.36163805019426 36.0 34.0 39.0 29.0 41.0 72-73 33.93999822352984 35.5 34.0 38.5 28.5 40.0 74-75 33.515585406796696 35.0 34.0 37.0 28.0 39.0 76-77 33.09137556175878 35.0 34.0 37.0 27.0 39.0 78-79 32.696104091170845 35.0 33.5 36.0 26.5 38.5 80-81 32.31446865079088 35.0 33.0 36.0 26.0 37.0 82-83 31.977619220608652 35.0 33.0 35.0 25.5 37.0 84-85 31.661347901719285 35.0 33.0 35.0 25.0 36.5 86-87 31.390413124122368 35.0 32.5 35.0 24.0 36.0 88-89 31.104257465416218 35.0 32.0 35.0 23.0 36.0 90-91 30.84451594681368 34.5 32.0 35.0 21.0 35.0 92-93 30.542746313075916 34.0 31.5 35.0 19.5 35.0 94-95 30.209094279666303 34.0 31.0 35.0 16.5 35.0 96-97 29.863352269041414 34.0 31.0 35.0 4.5 35.0 98-99 29.424680160521042 34.0 31.0 35.0 2.0 35.0 100-101 27.452328099067152 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1587199807678985 1101 2 0.2082224286392247 1101 3 0.20864849298158106 1101 4 0.18926177688842927 1101 5 0.13585327430005378 1101 6 0.07927722569029783 1101 7 0.11168048372761064 1101 8 0.10471559907718131 1101 9 0.12915708982549035 1101 10-11 0.11546777723190615 1101 12-13 0.13457325273194698 1101 14-15 0.11560881007607549 1101 16-17 0.1429649396171584 1101 18-19 0.1742717934528386 1101 20-21 0.210780068794719 1101 22-23 0.18838012562428474 1101 24-25 0.1672817935342792 1101 26-27 0.22514689245473818 1101 28-29 0.16976546785096502 1101 30-31 0.24093614228171845 1101 32-33 0.1348066042885563 1101 34-35 0.09906130301038729 1101 36-37 0.09057030177734049 1101 38-39 0.17723787672965585 1101 40-41 0.1472793935031831 1101 42-43 0.19683171398239097 1101 44-45 0.16785787215384573 1101 46-47 0.1667435323976818 1101 48-49 0.14061427776422875 1101 50-51 0.05516089987499839 1101 52-53 0.15548664718451732 1101 54-55 0.2030178530986504 1101 56-57 0.19834256692696073 1101 58-59 0.15357818071502294 1101 60-61 0.20250317571328935 1101 62-63 0.18407696585639854 1101 64-65 0.2089377959076799 1101 66-67 0.1051974891341203 1101 68-69 0.11022023959348815 1101 70-71 0.17079031200407258 1101 72-73 0.04776884443616325 1101 74-75 0.09567438639935233 1101 76-77 0.11653253848434275 1101 78-79 0.19967662941387943 1101 80-81 0.08715766632557376 1101 82-83 0.04066621825032968 1101 84-85 0.16353521462946574 1101 86-87 0.1562341449092095 1101 88-89 0.1604289355427042 1101 90-91 0.09673928336477289 1101 92-93 0.10378454839177564 1101 94-95 0.25817597896550737 1101 96-97 0.140681112619653 1101 98-99 0.13600088192606208 1101 100-101 0.3695304310556189 1102 1 0.15946263137056604 1102 2 0.23166254494505267 1102 3 0.1891206838577304 1102 4 0.15877659789310172 1102 5 0.13302644058131108 1102 6 0.19171280752893693 1102 7 0.17156126813593886 1102 8 0.19801618965304613 1102 9 0.1713117367946566 1102 10-11 0.2310013296967668 1102 12-13 0.24424872698346434 1102 14-15 0.24803730003130653 1102 16-17 0.2908872505606652 1102 18-19 0.21791782567501627 1102 20-21 0.2528433227867737 1102 22-23 0.36659405026356495 1102 24-25 0.31847732212956714 1102 26-27 0.23918953580944446 1102 28-29 0.35565593965034736 1102 30-31 0.287999094716632 1102 32-33 0.2052401032666964 1102 34-35 0.22501114188893467 1102 36-37 0.27878580562377664 1102 38-39 0.23588816249473155 1102 40-41 0.28030484757153573 1102 42-43 0.3101451180917181 1102 44-45 0.28362202325196506 1102 46-47 0.2945511220090964 1102 48-49 0.26867865386630285 1102 50-51 0.19899340815856448 1102 52-53 0.277412285308543 1102 54-55 0.44111527351534363 1102 56-57 0.34298881658770597 1102 58-59 0.3501859670263343 1102 60-61 0.33104958033209186 1102 62-63 0.3035101283602515 1102 64-65 0.2839263288004261 1102 66-67 0.2750568920827092 1102 68-69 0.3229196045776277 1102 70-71 0.2775574130717473 1102 72-73 0.2661436339413399 1102 74-75 0.2772689914891089 1102 76-77 0.30576255728140467 1102 78-79 0.35255128903786215 1102 80-81 0.35342019351201515 1102 82-83 0.33452962291515576 1102 84-85 0.30280495167692223 1102 86-87 0.3309014004058817 1102 88-89 0.30806780922147325 1102 90-91 0.27133875463457713 1102 92-93 0.25848468684353776 1102 94-95 0.221915319317187 1102 96-97 0.25073373169637136 1102 98-99 0.3241691682720287 1102 100-101 0.5496881427976739 1103 1 -0.012961961313585846 1103 2 0.11172113891856839 1103 3 0.029193468559206792 1103 4 0.05841915939579678 1103 5 0.07375917042723046 1103 6 0.036500576036964105 1103 7 0.014569092109880444 1103 8 0.08342606136428543 1103 9 0.09972676218156096 1103 10-11 0.09774829604719315 1103 12-13 0.09076436920927478 1103 14-15 0.09379743858163891 1103 16-17 0.05036528354266068 1103 18-19 -0.03454952992860427 1103 20-21 0.023572085332411064 1103 22-23 -0.010253319301902764 1103 24-25 -0.007653263085366291 1103 26-27 0.047700539345683524 1103 28-29 0.0432159736775759 1103 30-31 0.06903330123750351 1103 32-33 -0.011888143529894535 1103 34-35 0.00850736341687508 1103 36-37 -0.01956681107778735 1103 38-39 0.010062182018430121 1103 40-41 0.10713066401523008 1103 42-43 0.05783550082175282 1103 44-45 0.08464207719198669 1103 46-47 0.029546525281354263 1103 48-49 0.12052344850128804 1103 50-51 0.03755346888878108 1103 52-53 0.053262107733701214 1103 54-55 0.09336004068440928 1103 56-57 0.12009768464826465 1103 58-59 0.14791850232195713 1103 60-61 0.1100631340031839 1103 62-63 0.20793862730929646 1103 64-65 0.25864966682585333 1103 66-67 0.2582740674411994 1103 68-69 0.25942811329233706 1103 70-71 0.16958657272887478 1103 72-73 0.16647208062882868 1103 74-75 0.2228065416048821 1103 76-77 0.23380564826609884 1103 78-79 0.237429771392911 1103 80-81 0.15291512566736287 1103 82-83 0.24603593817057146 1103 84-85 0.2671414016298108 1103 86-87 0.16062697003772541 1103 88-89 0.14565340181788144 1103 90-91 0.04419460048970336 1103 92-93 0.1979433029423845 1103 94-95 0.09367348184679614 1103 96-97 -0.0792592492338855 1103 98-99 -0.024062140593741788 1103 100-101 0.2684735004078327 1104 1 0.08054938784918875 1104 2 0.08313028772202102 1104 3 0.09766718638582006 1104 4 0.09972398629165014 1104 5 0.06668672030042444 1104 6 0.08881429625404991 1104 7 0.12382210541811389 1104 8 0.08583894902307776 1104 9 0.13614231671618882 1104 10-11 0.16050597265302002 1104 12-13 0.16729156648711552 1104 14-15 0.16328075940231912 1104 16-17 0.13929270642177016 1104 18-19 0.29709608933564624 1104 20-21 0.25420660755450797 1104 22-23 0.27106827828372104 1104 24-25 0.20575208336570228 1104 26-27 0.2432079367512685 1104 28-29 0.18452154759746975 1104 30-31 0.1545406612918825 1104 32-33 0.13588516455402555 1104 34-35 0.16623999604166073 1104 36-37 0.1032090182122758 1104 38-39 0.09056280018082674 1104 40-41 0.08862855927498714 1104 42-43 0.17868662266511137 1104 44-45 0.20379652067775567 1104 46-47 0.08562300560287639 1104 48-49 0.13725323869958572 1104 50-51 0.030680100669421506 1104 52-53 0.06898490952207936 1104 54-55 0.22795061548205098 1104 56-57 0.14000512836934575 1104 58-59 0.13251766789610286 1104 60-61 0.16980694210764113 1104 62-63 0.15638444378790695 1104 64-65 0.21593221746474 1104 66-67 0.14566392808387008 1104 68-69 0.19811723550173355 1104 70-71 0.22541970996839922 1104 72-73 0.14655679315530534 1104 74-75 0.07889366701901679 1104 76-77 0.11539019831484865 1104 78-79 0.10240573589432955 1104 80-81 0.15470518203032668 1104 82-83 0.18080206361832296 1104 84-85 0.16770913990246683 1104 86-87 0.11851535717546469 1104 88-89 0.2144119225608705 1104 90-91 0.25006228759121285 1104 92-93 0.24358647196778094 1104 94-95 0.3245733843601606 1104 96-97 0.35740263647890913 1104 98-99 0.42326874250072777 1104 100-101 0.6278783463527979 1105 1 -0.17277635415649684 1105 2 0.014864857049040836 1105 3 -0.04967114113877358 1105 4 0.08030259453843769 1105 5 0.09309551721720766 1105 6 0.15737457742741157 1105 7 0.07370904465659578 1105 8 0.06144984738412518 1105 9 0.07123438117969982 1105 10-11 -0.023686460637215134 1105 12-13 0.010098489307146963 1105 14-15 0.054830883496997274 1105 16-17 0.07019329631893356 1105 18-19 0.08878960517470347 1105 20-21 -0.0011472277578121748 1105 22-23 0.122906208342485 1105 24-25 0.15376589438381671 1105 26-27 0.2140038222320655 1105 28-29 0.26022517981235893 1105 30-31 0.20302517875953896 1105 32-33 0.24633818487750148 1105 34-35 0.12883174297386546 1105 36-37 0.16904594737312095 1105 38-39 0.1326828378039906 1105 40-41 0.14515477166836632 1105 42-43 0.08324743886033303 1105 44-45 0.05799117055718739 1105 46-47 -0.003802147486446472 1105 48-49 0.025062745905714223 1105 50-51 -0.02216789770638883 1105 52-53 0.07212522059977289 1105 54-55 0.3528164309196242 1105 56-57 0.2526326251584763 1105 58-59 0.17622254228366074 1105 60-61 0.20465134441073474 1105 62-63 0.3003926770437033 1105 64-65 0.28330972516518216 1105 66-67 0.37256027654738233 1105 68-69 0.3501519614121378 1105 70-71 0.3108310956848328 1105 72-73 0.30839722913682266 1105 74-75 0.20293034568567236 1105 76-77 0.16339516994789705 1105 78-79 0.21979362772673738 1105 80-81 0.3208556457731575 1105 82-83 0.3485562443654757 1105 84-85 0.3950241638166183 1105 86-87 0.370375489906003 1105 88-89 0.367793030257193 1105 90-91 0.2544614447969167 1105 92-93 0.27268748076106775 1105 94-95 0.24153864079550047 1105 96-97 0.1218009036161547 1105 98-99 0.016961197613571954 1105 100-101 0.21502775623496362 1106 1 -0.1426498173172277 1106 2 -0.011378378917790144 1106 3 -0.014227122229804934 1106 4 0.1147627210025135 1106 5 0.10290266799178482 1106 6 0.14008509014779236 1106 7 0.08546869985158878 1106 8 0.11949719743542175 1106 9 -0.011242922472334271 1106 10-11 0.06618258816569522 1106 12-13 0.1756393330316044 1106 14-15 0.1655363682153066 1106 16-17 0.06685275529329715 1106 18-19 0.2433676216727534 1106 20-21 0.21370835967921664 1106 22-23 0.23326333474107486 1106 24-25 0.18624240975766782 1106 26-27 0.29485466544717553 1106 28-29 0.25630428413430195 1106 30-31 0.23317615222245536 1106 32-33 0.3663184294135178 1106 34-35 0.1448882419030184 1106 36-37 0.24816209349321383 1106 38-39 0.269191577989929 1106 40-41 0.31501246926546145 1106 42-43 0.4187673143851498 1106 44-45 0.3629881265906718 1106 46-47 0.3224834129550871 1106 48-49 0.34622868210126256 1106 50-51 0.24398416479315443 1106 52-53 0.3438845483561437 1106 54-55 0.5146740751563357 1106 56-57 0.5171751448836233 1106 58-59 0.5191558709277473 1106 60-61 0.5366689606090063 1106 62-63 0.5475197668904386 1106 64-65 0.470918052778444 1106 66-67 0.5511250523974454 1106 68-69 0.5712694488178798 1106 70-71 0.5698236519133246 1106 72-73 0.5054134143070073 1106 74-75 0.5925891061242936 1106 76-77 0.5168711129105148 1106 78-79 0.3560992953255919 1106 80-81 0.5458754133644916 1106 82-83 0.7058876559170244 1106 84-85 0.6869261214145332 1106 86-87 0.7170471975105848 1106 88-89 0.7646388260664629 1106 90-91 0.5638906465015374 1106 92-93 0.7176590542370853 1106 94-95 0.7721627024412818 1106 96-97 0.7131108036624845 1106 98-99 0.7256807670439009 1106 100-101 1.096709323380498 1107 1 0.00929046169989789 1107 2 0.03251114361669494 1107 3 0.045907086455997614 1107 4 0.029955313643036163 1107 5 -0.009285405340655473 1107 6 4.4341133924774567E-4 1107 7 -0.00912918878508151 1107 8 0.018763567206939058 1107 9 -0.047289389641612445 1107 10-11 0.005858648538428213 1107 12-13 0.08844956856149366 1107 14-15 0.08763506712751479 1107 16-17 0.10509340741950979 1107 18-19 0.14991888314381896 1107 20-21 0.11017376429639825 1107 22-23 0.19277544005159797 1107 24-25 0.1743405651529244 1107 26-27 0.15607237868787394 1107 28-29 0.23012309064404235 1107 30-31 0.18767401962432473 1107 32-33 0.18198377591439652 1107 34-35 0.23700006992578437 1107 36-37 0.19013151301829367 1107 38-39 0.2112256725018753 1107 40-41 0.2471631728669621 1107 42-43 0.14049528650813414 1107 44-45 0.2041114772902688 1107 46-47 0.18081039839483282 1107 48-49 0.10074399027948289 1107 50-51 0.09521248955511652 1107 52-53 0.17141309328953014 1107 54-55 0.3837341084849797 1107 56-57 0.23574792468455286 1107 58-59 0.2552310139097642 1107 60-61 0.1964511773699158 1107 62-63 0.22928112648277477 1107 64-65 0.2511991816231358 1107 66-67 0.17730224597948308 1107 68-69 0.18982319964359107 1107 70-71 0.13305149550986073 1107 72-73 0.1375274422793069 1107 74-75 0.11617741792184688 1107 76-77 0.18394269185664314 1107 78-79 0.20035528004123648 1107 80-81 0.26555022670701334 1107 82-83 0.31473818816952104 1107 84-85 0.31707242982274764 1107 86-87 0.3227300671388704 1107 88-89 0.3999106933589225 1107 90-91 0.3592696054548341 1107 92-93 0.45525854460171544 1107 94-95 0.5289391058061454 1107 96-97 0.5791721116677735 1107 98-99 0.5698444597591852 1107 100-101 0.8186440848771461 1108 1 0.2596553502272343 1108 2 0.3004010671185853 1108 3 0.26772198969412386 1108 4 0.1946781528071142 1108 5 0.2676401785731244 1108 6 0.2666412088953507 1108 7 0.23745565360177068 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4232.0 8 4651.0 9 5280.0 10 5891.0 11 6974.0 12 7733.0 13 8591.0 14 8836.0 15 9837.0 16 11571.0 17 13856.0 18 13537.0 19 11525.0 20 12170.0 21 13183.0 22 13707.0 23 15220.0 24 17301.0 25 20673.0 26 23994.0 27 28411.0 28 34584.0 29 41919.0 30 52073.0 31 65136.0 32 84337.0 33 113279.0 34 164988.0 35 262488.0 36 499809.0 37 1050939.0 38 1160914.0 39 169430.0 40 9.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39329855292367 17.94701581838986 13.553528847317661 25.106156781368817 2 15.885043385152581 19.896120232968222 38.292298495839944 25.926537886039252 3 18.399369297107683 24.102623486445328 28.977044431211908 28.52096278523508 4 13.55981396667452 16.7363609501715 35.83582229604814 33.868002787105844 5 15.446722219892528 35.73697883905339 33.54500270577358 15.271296235280493 6 33.17195773005694 34.50699374695533 18.134079093428312 14.186969429559412 7 29.781442380956065 30.56751906292731 21.41362028780135 18.237418268315274 8 27.299496247691778 33.59412869720797 20.270851107271636 18.835523947828612 9 27.009898440038594 16.2690602746167 19.472139767111383 37.248901518233325 10-11 25.706150991206343 25.333460450265978 27.393790495904536 21.56659806262314 12-13 26.364286152815623 22.43474621489862 27.52582174622039 23.675145886065366 14-15 23.718280709434865 23.79808826278612 24.660708701057505 27.822922326721518 16-17 24.352038472382233 26.692586500643937 25.22422441124672 23.731150615727103 18-19 24.37199391985051 26.063480125501993 26.263347754072708 23.30117820057479 20-21 24.70700349902263 25.5796437928322 26.00045751745736 23.71289519068781 22-23 24.666101536684273 25.218638406173692 25.950574091186617 24.164685965955414 24-25 24.604135880562538 25.514857623925625 25.704830476212344 24.176176019299493 26-27 24.43321441811927 25.728736797984187 25.65490196763521 24.183146816261335 28-29 24.463307017323842 25.64062056816575 25.848463260597125 24.047609153913278 30-31 24.456143582187295 25.46118545050873 25.738298532911656 24.34437243439232 32-33 24.347382566550905 25.453078286636504 25.757322758469897 24.44221638834269 34-35 24.735629412416618 25.520466723976288 25.481066358352876 24.262837505254215 36-37 24.645032539541806 25.36319734207893 25.596267090419257 24.395503027960007 38-39 24.430834043077766 25.4799165824664 25.832210177847436 24.2570391966084 40-41 24.55946927689376 25.27726259554024 25.876457247582607 24.28681087998339 42-43 24.547127919190388 25.30845511781824 25.662035857431736 24.482381105559643 44-45 24.4947909056436 25.51487324243835 25.5053775462597 24.48495830565835 46-47 24.552812661813057 25.518943434834956 25.458079891199244 24.470164012152747 48-49 24.667332845345804 25.48519029392306 25.457787789314175 24.389689071416967 50-51 24.448676251535044 25.542876493648087 25.51085927672034 24.49758797809653 52-53 24.72574479915904 25.439459705524435 25.324880978883176 24.50991451643335 54-55 24.597205293531026 25.2974666359205 25.4726723507139 24.63265571983457 56-57 24.453662698178487 25.68488770535651 25.588806460053725 24.272643136411283 58-59 24.635602927854297 25.293633938353345 25.564620180033593 24.50614295375877 60-61 24.68563953349811 25.29261804574755 25.671528858400006 24.35021356235433 62-63 24.570114951708007 25.42995367766729 25.575622646246394 24.424308724378317 64-65 24.448077118160928 25.290159501161614 25.561424648618676 24.700338732058785 66-67 24.38106593811358 25.570858242588614 25.445758796889205 24.6023170224086 68-69 24.22240528909648 25.382951388507614 25.728231540719875 24.666411781676032 70-71 24.284964967548355 25.362826077976546 25.665122384265864 24.68708657020923 72-73 24.480605970307934 25.309270516148402 25.503521524838597 24.706601988705064 74-75 24.35156323805119 25.427741321407165 25.40827314943482 24.812422291106824 76-77 24.510986409237535 25.295050618083025 25.519641293931844 24.674321678747592 78-79 24.361215064645304 25.401367403194268 25.60697702567968 24.630440506480753 80-81 24.30829198610089 25.519953172176972 25.60816087867803 24.563593963044102 82-83 24.458125607953896 25.40820563702089 25.454346499158788 24.679322255866428 84-85 24.307007749782642 25.34999189468153 25.485018139315883 24.857982216219945 86-87 24.426635668649297 25.23460634497739 25.674284239774124 24.664473746599196 88-89 24.30925926896528 25.16923625238571 25.65764597445714 24.863858504191878 90-91 24.28892032036816 25.3568391439629 25.72954134095675 24.62469919471219 92-93 24.4129393469671 25.14987906335882 25.722524667829855 24.714656921844227 94-95 24.34664731370979 25.114119564268826 25.656542528334974 24.88269059368641 96-97 24.513554199001376 25.105696965610164 25.57949609361098 24.80125274177748 98-99 24.179087968321454 25.113711511526333 25.582989584346393 25.12421093580582 100-101 24.12173316349858 24.9430353559101 25.905597380263128 25.02963410032819 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 230.0 1 178.0 2 189.5 3 221.0 4 159.5 5 150.5 6 207.5 7 371.0 8 618.0 9 750.5 10 739.0 11 738.0 12 799.5 13 868.0 14 956.0 15 1036.5 16 1031.0 17 1045.5 18 1123.5 19 1179.5 20 1288.0 21 1515.0 22 1818.5 23 2299.0 24 2883.0 25 3679.0 26 5278.0 27 7509.5 28 9885.0 29 13100.5 30 17723.0 31 22771.0 32 27969.0 33 33755.0 34 40188.5 35 46587.0 36 54061.5 37 62703.0 38 72278.5 39 79888.0 40 86295.5 41 98490.0 42 111113.5 43 122690.0 44 134734.0 45 145647.0 46 152965.0 47 160327.0 48 173966.0 49 184118.5 50 188511.5 51 190112.0 52 188316.5 53 187421.0 54 185572.5 55 175857.5 56 162300.5 57 148927.0 58 133700.5 59 116596.0 60 97990.0 61 79116.5 62 62906.0 63 49284.5 64 38054.5 65 28850.0 66 21239.0 67 16726.5 68 12871.0 69 9242.0 70 6660.0 71 4697.5 72 3448.5 73 2648.0 74 2031.0 75 1577.0 76 1089.5 77 652.5 78 409.0 79 272.0 80 188.0 81 130.5 82 97.5 83 78.5 84 63.5 85 47.5 86 38.0 87 34.0 88 29.0 89 26.0 90 23.0 91 19.5 92 18.0 93 16.0 94 12.5 95 10.5 96 9.0 97 6.0 98 6.5 99 6.0 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022530232428165233 2 0.03475593994732466 3 0.024700919273403746 4 0.023054191321843498 5 0.04241571996443068 6 0.023453398097979316 7 0.027121110353727145 8 0.04026998354270066 9 0.026297746377947018 10-11 0.028406057164414307 12-13 0.026297746377947018 14-15 0.035803857734681185 16-17 0.04365076592810086 18-19 0.04482343583299983 20-21 0.0385109786853522 22-23 0.04466125808019465 24-25 0.019873012324511198 26-27 0.025736361849006024 28-29 0.026734378789345568 30-31 0.027445465859337496 32-33 0.03657732086344433 34-35 0.04091869455392136 36-37 0.030127636386500026 38-39 0.01589341977490726 40-41 0.018363511702247634 42-43 0.019985289230299394 44-45 0.02169439324063087 46-47 0.015544113845788418 48-49 0.025324679861115963 50-51 0.018188858737688215 52-53 0.027345664165303544 54-55 0.02355319979201327 56-57 0.023016765686580763 58-59 0.022904488780792563 60-61 0.020072615712579105 62-63 0.022892013569038323 64-65 0.019922913171528174 66-67 0.018363511702247634 68-69 0.022754786239741635 70-71 0.03614068845204579 72-73 0.025424481555149914 74-75 0.03514267151170624 76-77 0.04418720003353337 78-79 0.027021308659693186 80-81 0.023166468227631694 82-83 0.016317576974551568 84-85 0.032922083819450755 86-87 0.020209843041875793 88-89 0.031711988279289054 90-91 0.024838146602700437 92-93 0.04286482758758347 94-95 0.07603641564211912 96-97 0.09806763960011457 98-99 0.07401543133793152 100-101 0.08161283529626631 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4007948.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.70383459819541 #Duplication Level Percentage of deduplicated Percentage of total 1 76.55086784509066 38.81422541565793 2 11.75881319812012 11.924338389371195 3 4.210413527771714 6.404523333064242 4 2.095649355028511 4.25029833092722 5 1.2825983668794199 3.251632772508483 6 0.8261449802391568 2.513323105930538 7 0.5692078711517549 2.0202715225608654 8 0.43409240545477573 1.7608119621209382 9 0.3287403578745435 1.5001557058280177 >10 1.764948711448544 16.203371224703446 >50 0.11443574477560445 4.025164227189537 >100 0.06003935175938767 5.558281876715741 >500 0.0029127900616833127 0.9704710176708122 >1k 0.0011354943441191426 0.8031311157510684 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 2.4950423508488634E-5 0.0 0.0 0.0 6 2.4950423508488634E-5 2.4950423508488634E-5 0.0 0.0 0.0 7 2.4950423508488634E-5 2.4950423508488634E-5 0.0 0.0 0.0 8 2.4950423508488634E-5 2.4950423508488634E-5 0.0 0.0 0.0 9 2.4950423508488634E-5 2.4950423508488634E-5 0.0 0.0 0.0 10-11 2.4950423508488634E-5 2.4950423508488634E-5 0.0 0.0 0.0 12-13 2.4950423508488634E-5 2.4950423508488634E-5 0.0 2.4950423508488634E-5 2.4950423508488634E-5 14-15 2.4950423508488634E-5 2.4950423508488634E-5 0.0 2.4950423508488634E-5 4.990084701697727E-5 16-17 2.4950423508488634E-5 2.4950423508488634E-5 0.0 2.4950423508488634E-5 7.48512705254659E-5 18-19 2.4950423508488634E-5 2.4950423508488634E-5 0.0 2.4950423508488634E-5 7.48512705254659E-5 20-21 2.4950423508488634E-5 2.4950423508488634E-5 0.0 2.4950423508488634E-5 7.48512705254659E-5 22-23 2.4950423508488634E-5 2.4950423508488634E-5 0.0 2.4950423508488634E-5 7.48512705254659E-5 24-25 2.4950423508488634E-5 2.4950423508488634E-5 0.0 1.2475211754244317E-4 7.48512705254659E-5 26-27 2.4950423508488634E-5 2.4950423508488634E-5 0.0 2.4950423508488635E-4 7.48512705254659E-5 28-29 4.990084701697727E-5 2.4950423508488634E-5 0.0 5.114836819240169E-4 7.48512705254659E-5 30-31 4.990084701697727E-5 2.4950423508488634E-5 0.0 0.002133261209975778 7.48512705254659E-5 32-33 4.990084701697727E-5 2.4950423508488634E-5 0.0 0.005800973465723607 1.2475211754244317E-4 34-35 7.48512705254659E-5 3.742563526273295E-5 0.0 0.010092446309183652 1.2475211754244317E-4 36-37 7.48512705254659E-5 4.990084701697727E-5 0.0 0.017664899844009953 1.2475211754244317E-4 38-39 7.48512705254659E-5 4.990084701697727E-5 0.0 0.03011516117474578 1.2475211754244317E-4 40-41 7.48512705254659E-5 6.237605877122159E-5 0.0 0.045384820361940824 1.2475211754244317E-4 42-43 7.48512705254659E-5 7.48512705254659E-5 0.0 0.060629529125627385 1.2475211754244317E-4 44-45 9.980169403395454E-5 8.732648227971021E-5 0.0 0.07861878447524769 1.2475211754244317E-4 46-47 1.8712817631366473E-4 9.980169403395454E-5 0.0 0.10088703745657379 1.2475211754244317E-4 48-49 2.245538115763977E-4 9.980169403395454E-5 0.0 0.12616181647067276 1.497025410509318E-4 50-51 2.245538115763977E-4 9.980169403395454E-5 0.0 0.15298352174229807 1.497025410509318E-4 52-53 2.245538115763977E-4 9.980169403395454E-5 0.0 0.182237893306001 1.497025410509318E-4 54-55 2.4950423508488635E-4 9.980169403395454E-5 0.0 0.21376275340897638 1.497025410509318E-4 56-57 2.994050821018636E-4 9.980169403395454E-5 0.0 0.24984106580225093 1.7465296455942042E-4 58-59 2.994050821018636E-4 9.980169403395454E-5 0.0 0.2911963927675708 1.9960338806790907E-4 60-61 2.994050821018636E-4 9.980169403395454E-5 0.0 0.3372923001995036 1.9960338806790907E-4 62-63 2.994050821018636E-4 9.980169403395454E-5 0.0 0.38694364298139594 2.245538115763977E-4 64-65 2.994050821018636E-4 9.980169403395454E-5 0.0 0.44193437639410493 2.245538115763977E-4 66-67 2.994050821018636E-4 9.980169403395454E-5 0.0 0.5081028995386168 2.245538115763977E-4 68-69 3.243555056103522E-4 9.980169403395454E-5 0.0 0.5807834832188441 2.245538115763977E-4 70-71 3.243555056103522E-4 9.980169403395454E-5 0.0 0.6658020513240193 2.3702902333064203E-4 72-73 3.243555056103522E-4 9.980169403395454E-5 0.0 0.7587049532578767 2.7445465859337497E-4 74-75 3.243555056103522E-4 1.2475211754244317E-4 0.0 0.8591803087265604 2.994050821018636E-4 76-77 3.243555056103522E-4 1.2475211754244317E-4 0.0 0.9733659219131585 2.994050821018636E-4 78-79 3.243555056103522E-4 1.2475211754244317E-4 0.0 1.1047798025323683 2.994050821018636E-4 80-81 3.742563526273295E-4 1.2475211754244317E-4 0.0 1.2509518586568489 3.118802938561079E-4 82-83 3.742563526273295E-4 1.2475211754244317E-4 0.0 1.4097862547118876 3.243555056103522E-4 84-85 3.9920677613581814E-4 1.2475211754244317E-4 0.0 1.587096439374962 3.243555056103522E-4 86-87 4.2415719964430677E-4 1.2475211754244317E-4 0.0 1.7813978624473172 3.7425635262732947E-4 88-89 4.2415719964430677E-4 1.2475211754244317E-4 0.0 1.9974061539720576 4.2415719964430677E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4705 0.0 37.451897 1 GGTATCA 3365 0.0 33.31091 1 ATCAACG 5305 0.0 31.873884 3 TCAACGC 5340 0.0 31.842865 4 TATAACG 255 0.0 31.665764 2 CAACGCA 5530 0.0 30.921362 5 AACGCAG 5990 0.0 28.864672 6 TATCAAC 5990 0.0 28.863953 2 ACGCAGA 6925 0.0 24.96742 7 GTACATG 9055 0.0 24.128422 1 CGCAGAG 7465 0.0 23.35252 8 TATCACG 320 0.0 22.26499 2 TACATGG 9695 0.0 22.24279 2 ACATGGG 9830 0.0 21.501902 3 TACCTGG 3900 0.0 21.435287 2 GTACCTG 4020 0.0 21.266989 1 TAACGCA 390 0.0 20.704021 4 ATAACGC 390 0.0 19.486137 3 CATGGGG 8770 0.0 19.280611 4 CCTATAA 1975 0.0 18.03656 1 >>END_MODULE