FastQCFastQC Report
Fri 27 May 2016
SRR940216_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940216_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4007948
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA48750.040.267541
TCAACGC54050.035.5910574
ATCAACG54900.034.780463
CAACGCA55900.034.4972885
GGTATCA32050.032.9233931
AACGCAG61050.032.1318056
TATCAAC61350.031.666582
ACGCAGA70750.027.5921867
TATAACG3100.027.580572
CGCAGAG75600.025.8220528
GTACATG95700.025.628161
ATAACGC3350.025.521683
ACATGGG99300.024.3950963
TACATGG100500.024.104412
TAACGCA4150.022.8909324
CATGGGG88500.021.2536494
GTACCTG39300.020.3187241
TACCTGG38750.020.103172
GCAGAGT100300.019.3210169
AGAGTAC84900.018.29420510-11
Produced by FastQC (version 0.11.5)