FastQCFastQC Report
Fri 27 May 2016
SRR940215_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940215_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504999
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33750.041.5301441
GTATCAA56400.037.909551
TCAACGC66550.032.5546264
ATCAACG67000.032.3366933
CAACGCA69600.031.2621175
AACGCAG73500.029.5275256
TATCAAC72800.028.65222
GTACATG117000.025.5028671
ACGCAGA87050.024.8773377
ACATGGG127250.023.85883
TACATGG123550.023.4975912
CGCAGAG92600.023.3347648
CATGGGG108100.019.8219684
TTATAGG20150.018.8634243
GCTTATA21350.018.6937581
GCAGAGT115900.018.2345099
AGAGTAC99150.016.81360210-11
TATAGGA24750.016.7009034
GCGTAAA2002.7566837E-416.6296561
TATAAGC18050.016.5832213
Produced by FastQC (version 0.11.5)