##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940215_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4504999 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.848308512388126 33.0 31.0 34.0 30.0 34.0 2 30.832869441258477 33.0 31.0 34.0 26.0 34.0 3 31.68618994144061 34.0 31.0 34.0 28.0 34.0 4 35.4195106369613 37.0 35.0 37.0 33.0 37.0 5 35.57898414627839 37.0 35.0 37.0 35.0 37.0 6 35.727959984008876 37.0 36.0 37.0 35.0 37.0 7 35.698345992973586 37.0 37.0 37.0 35.0 37.0 8 35.69238994281686 37.0 37.0 37.0 35.0 37.0 9 37.43839366002079 39.0 38.0 39.0 35.0 39.0 10-11 37.370995309876875 39.0 38.0 39.0 35.0 39.0 12-13 37.29456965473244 39.0 38.0 39.0 35.0 39.0 14-15 38.71094976935622 41.0 39.0 41.0 35.0 41.0 16-17 38.62013299004062 41.0 39.0 41.0 35.0 41.0 18-19 38.544794793517156 41.0 39.0 41.0 34.5 41.0 20-21 38.46393850475883 40.0 39.0 41.0 34.0 41.0 22-23 38.36207166749648 40.0 38.5 41.0 34.0 41.0 24-25 38.254801277425365 40.0 38.0 41.0 34.0 41.0 26-27 38.05968880348253 40.0 38.0 41.0 33.5 41.0 28-29 37.88947522518873 40.0 38.0 41.0 33.0 41.0 30-31 37.87667322456676 40.0 38.0 41.0 33.0 41.0 32-33 37.91108055739857 40.0 38.0 41.0 33.0 41.0 34-35 37.92215714143333 40.0 38.0 41.0 33.0 41.0 36-37 38.03573574600128 40.0 38.0 41.0 33.5 41.0 38-39 37.96041519654055 40.0 38.0 41.0 33.0 41.0 40-41 37.834378431604534 40.0 38.0 41.0 33.0 41.0 42-43 37.570473156597814 40.0 38.0 41.0 32.5 41.0 44-45 37.51908546483584 40.0 38.0 41.0 32.5 41.0 46-47 37.36405735051218 40.0 37.5 41.0 32.0 41.0 48-49 37.186227011371145 40.0 37.0 41.0 32.0 41.0 50-51 36.2392898422397 39.0 36.0 40.0 30.0 40.5 52-53 36.31137864847473 39.0 36.0 40.0 30.5 41.0 54-55 36.666278061326985 40.0 36.0 41.0 31.0 41.0 56-57 36.480235511706 39.5 35.5 41.0 31.0 41.0 58-59 36.208580512448506 39.0 35.0 41.0 30.0 41.0 60-61 35.93102695472297 39.0 35.0 41.0 30.0 41.0 62-63 35.574126875499864 38.0 35.0 40.0 29.0 41.0 64-65 35.18473433623404 37.0 35.0 40.0 29.0 41.0 66-67 34.80076788030364 37.0 35.0 39.5 29.0 41.0 68-69 34.40812617272501 36.0 34.0 39.0 28.0 41.0 70-71 33.98938956479236 36.0 34.0 39.0 28.0 40.5 72-73 33.55045361830269 35.0 34.0 37.5 27.5 40.0 74-75 33.117798139355855 35.0 34.0 37.0 26.0 39.0 76-77 32.68152012908327 35.0 33.0 36.5 26.0 39.0 78-79 32.26395644038989 35.0 33.0 36.0 25.5 38.0 80-81 31.89376745699611 35.0 33.0 35.5 24.5 37.0 82-83 31.563836751129134 35.0 32.5 35.0 24.0 37.0 84-85 31.281594402129723 35.0 32.0 35.0 23.0 36.0 86-87 30.99280543680476 35.0 32.0 35.0 21.0 36.0 88-89 30.69394854471666 34.5 32.0 35.0 19.5 35.5 90-91 30.423991548055838 34.0 31.0 35.0 18.0 35.0 92-93 30.138112350302407 34.0 31.0 35.0 15.0 35.0 94-95 29.81712748437902 34.0 31.0 35.0 4.5 35.0 96-97 29.43964826629262 34.0 30.5 35.0 2.0 35.0 98-99 28.94039743405048 34.0 30.0 35.0 2.0 35.0 100-101 26.893230275966765 32.0 26.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.6003985827476548 1101 2 1.7097237594927606 1101 3 0.9113708656322181 1101 4 0.5837427767550736 1101 5 0.4285052223515038 1101 6 0.31309151098633947 1101 7 0.3582200017273678 1101 8 0.3470673682182692 1101 9 0.3843199018958714 1101 10-11 0.4101260964267297 1101 12-13 0.4590663315572172 1101 14-15 0.5452859578586313 1101 16-17 0.5144384300267504 1101 18-19 0.5825954623680403 1101 20-21 0.5737587226712932 1101 22-23 0.5351033527108342 1101 24-25 0.556870249375585 1101 26-27 0.6386069888072754 1101 28-29 0.6737608264189845 1101 30-31 0.615478808648902 1101 32-33 0.655461498089771 1101 34-35 0.6227226371111954 1101 36-37 0.6289451164475608 1101 38-39 0.6884626229693396 1101 40-41 0.6283461066782863 1101 42-43 0.8365735731258397 1101 44-45 0.7309802943267911 1101 46-47 0.7513315345326745 1101 48-49 0.7522259047998645 1101 50-51 0.5781061330548454 1101 52-53 0.6828942998933556 1101 54-55 0.689901875779988 1101 56-57 0.7580174323998534 1101 58-59 0.7109699667942806 1101 60-61 0.5766685848814959 1101 62-63 0.626946982533525 1101 64-65 0.6318291845749044 1101 66-67 0.5826182320603408 1101 68-69 0.5769436981868807 1101 70-71 0.5437036850186843 1101 72-73 0.5463721000086892 1101 74-75 0.48979817053547947 1101 76-77 0.6112218384700583 1101 78-79 0.6560613526570833 1101 80-81 0.6961822562432793 1101 82-83 0.7378752209618931 1101 84-85 0.6581211044794912 1101 86-87 0.6355925247288781 1101 88-89 0.7767735690167115 1101 90-91 0.6947701500037518 1101 92-93 0.647064910727341 1101 94-95 0.6279098411307125 1101 96-97 0.6327134458687276 1101 98-99 0.6917910475232496 1101 100-101 0.7097621770260609 1102 1 0.3243593944008758 1102 2 0.9333189983784251 1102 3 0.4569919098714017 1102 4 0.30452547661337803 1102 5 0.2810724258239077 1102 6 0.18142550564410698 1102 7 0.2155388607503852 1102 8 0.2755893291953839 1102 9 0.22114165572825328 1102 10-11 0.26437753645408435 1102 12-13 0.24618859937832838 1102 14-15 0.31965760743361926 1102 16-17 0.3598618476038524 1102 18-19 0.3094191040050305 1102 20-21 0.4151883071645841 1102 22-23 0.37387756153231777 1102 24-25 0.35767132799309564 1102 26-27 0.4650688714898976 1102 28-29 0.4113776582059785 1102 30-31 0.38062751838122466 1102 32-33 0.36015372665824685 1102 34-35 0.36556182408789795 1102 36-37 0.44682022177204317 1102 38-39 0.37135890911889646 1102 40-41 0.40468740304279294 1102 42-43 0.6255541362475512 1102 44-45 0.47371135739395953 1102 46-47 0.5431254600438322 1102 48-49 0.509783314874241 1102 50-51 0.33859876310785353 1102 52-53 0.44065201749675253 1102 54-55 0.6218019596707052 1102 56-57 0.5986243464282026 1102 58-59 0.5982000884621712 1102 60-61 0.6065630509397408 1102 62-63 0.6725138824817236 1102 64-65 0.6370565128716379 1102 66-67 0.6622418251650757 1102 68-69 0.5632197671479986 1102 70-71 0.4950149912424706 1102 72-73 0.5883785517422737 1102 74-75 0.5678069859110906 1102 76-77 0.5151313658628567 1102 78-79 0.582102326052869 1102 80-81 0.6321606831026436 1102 82-83 0.6056344430125016 1102 84-85 0.4438253122418061 1102 86-87 0.38954752805657833 1102 88-89 0.5095428729714335 1102 90-91 0.46922476154283643 1102 92-93 0.4990071494396382 1102 94-95 0.32375240929323823 1102 96-97 0.3345800604198672 1102 98-99 0.3011200627505737 1102 100-101 0.5197391321092226 1103 1 0.29706955419262115 1103 2 1.3988060217562577 1103 3 0.6643416751355282 1103 4 0.3309595325921464 1103 5 0.26493184638037093 1103 6 0.16571691327812488 1103 7 0.19513706299871103 1103 8 0.22409573875718536 1103 9 0.26558642916971564 1103 10-11 0.2304318381810262 1103 12-13 0.2840099594603771 1103 14-15 0.2522550864485069 1103 16-17 0.3281519675295712 1103 18-19 0.2360149425148137 1103 20-21 0.2545714545655997 1103 22-23 0.24303260060456466 1103 24-25 0.31584263729760664 1103 26-27 0.3146511537827763 1103 28-29 0.2857752576757733 1103 30-31 0.35026705455423723 1103 32-33 0.20231029787657917 1103 34-35 0.31022366205841223 1103 36-37 0.3149215324121357 1103 38-39 0.2818537633256142 1103 40-41 0.41700609068911376 1103 42-43 0.5709308287271782 1103 44-45 0.4107937026862558 1103 46-47 0.2635427556547896 1103 48-49 0.37340823265998324 1103 50-51 0.3228650654698271 1103 52-53 0.3864529166245916 1103 54-55 0.42961678671207437 1103 56-57 0.5726805834975721 1103 58-59 0.5107821452170924 1103 60-61 0.5254486414585813 1103 62-63 0.41715335565530864 1103 64-65 0.414785081096241 1103 66-67 0.40979086391980246 1103 68-69 0.39970777583688033 1103 70-71 0.4951091438875608 1103 72-73 0.5537412142492997 1103 74-75 0.5976672027803218 1103 76-77 0.5162220434595071 1103 78-79 0.5805816773603638 1103 80-81 0.5059702459118078 1103 82-83 0.6348800689622216 1103 84-85 0.5644752647402385 1103 86-87 0.4913271043980245 1103 88-89 0.5496578217276991 1103 90-91 0.5838770110004106 1103 92-93 0.6299303556569242 1103 94-95 0.5004837910722877 1103 96-97 0.44689307811766454 1103 98-99 0.4841252110638301 1103 100-101 0.6408695812447469 1104 1 0.46949435634161674 1104 2 1.497315568278367 1104 3 0.7927950855033608 1104 4 0.45165901608165626 1104 5 0.32642287858149643 1104 6 0.2169417569022798 1104 7 0.25336108194225204 1104 8 0.2290127160857054 1104 9 0.20392792893098743 1104 10-11 0.14308966083128638 1104 12-13 0.18996303223965327 1104 14-15 0.2564010356269577 1104 16-17 0.20498061240087395 1104 18-19 0.20200795925438086 1104 20-21 0.26636764270852353 1104 22-23 0.21462343845113452 1104 24-25 0.2552912466738846 1104 26-27 0.21632565330774156 1104 28-29 0.262409186290256 1104 30-31 0.23219792847455523 1104 32-33 0.2510216741286726 1104 34-35 0.22038598187051406 1104 36-37 0.3509462101356391 1104 38-39 0.4506795700417001 1104 40-41 0.3886678824162644 1104 42-43 0.42917697177792036 1104 44-45 0.29900294007371286 1104 46-47 0.20276439701034832 1104 48-49 0.27767167471290577 1104 50-51 0.22384063611505667 1104 52-53 0.23039018784805876 1104 54-55 0.360488536819382 1104 56-57 0.35254770623029685 1104 58-59 0.41229586819192576 1104 60-61 0.2701441606938957 1104 62-63 0.3618858287808422 1104 64-65 0.4514242905602899 1104 66-67 0.4489434804722521 1104 68-69 0.25228574673674586 1104 70-71 0.27647154279183894 1104 72-73 0.3089446595816554 1104 74-75 0.268923225119039 1104 76-77 0.3353514387419452 1104 78-79 0.373781860140447 1104 80-81 0.26883441956736576 1104 82-83 0.14603996295941357 1104 84-85 0.17167988680984791 1104 86-87 0.10228287788574875 1104 88-89 0.15230560111839964 1104 90-91 0.05853381606230812 1104 92-93 0.09219217487342846 1104 94-95 0.09774624929570663 1104 96-97 -0.018341630326386138 1104 98-99 0.04489717963915396 1104 100-101 -0.02188318818709334 1105 1 0.06970562546476344 1105 2 1.0792004451588504 1105 3 0.4359016842237331 1105 4 0.21657838546394714 1105 5 0.06507367390591412 1105 6 -0.09681772379097708 1105 7 -0.012924092655531183 1105 8 -0.07157436409411844 1105 9 -0.18086442776835554 1105 10-11 -0.1510703565735767 1105 12-13 -0.2401073662182469 1105 14-15 -0.17916216989743106 1105 16-17 -0.31249573102311246 1105 18-19 -0.35200080725037 1105 20-21 -0.33816975177646924 1105 22-23 -0.3319621291735899 1105 24-25 -0.32594855299956293 1105 26-27 -0.23236077682830825 1105 28-29 -0.3102738912419625 1105 30-31 -0.35202224197655596 1105 32-33 -0.4183366745566488 1105 34-35 -0.34870898333529965 1105 36-37 -0.27274527010913374 1105 38-39 -0.26067525802932323 1105 40-41 -0.3048637781643393 1105 42-43 -0.17316280437636777 1105 44-45 -0.15352373331568003 1105 46-47 -0.2546123973338936 1105 48-49 -0.437809463808037 1105 50-51 -0.5374418195479933 1105 52-53 -0.4472743365123577 1105 54-55 -0.21289130015054525 1105 56-57 -0.4033944539210097 1105 58-59 -0.4028894543772168 1105 60-61 -0.5883737450384103 1105 62-63 -0.43125307565496485 1105 64-65 -0.37121404756041443 1105 66-67 -0.3934410996243187 1105 68-69 -0.4988945613308289 1105 70-71 -0.5109764105514927 1105 72-73 -0.716004433133044 1105 74-75 -0.7979044054805797 1105 76-77 -0.8207241733410271 1105 78-79 -0.6708985230017674 1105 80-81 -0.7563929332733892 1105 82-83 -0.7106966631254643 1105 84-85 -0.6720164539845079 1105 86-87 -0.7062939467418659 1105 88-89 -0.8278746955410625 1105 90-91 -1.0094715298508348 1105 92-93 -0.9418778808800035 1105 94-95 -1.0142187517511907 1105 96-97 -1.2509151830136283 1105 98-99 -1.5434749407185606 1105 100-101 -1.631722044489866 1106 1 0.2521261945359363 1106 2 1.2840255109937004 1106 3 0.48909997002967387 1106 4 0.22588555982303404 1106 5 0.07776151038433454 1106 6 0.16818804909602392 1106 7 0.1793365326517815 1106 8 0.2628863402809216 1106 9 0.07686648574679822 1106 10-11 0.1634624760902028 1106 12-13 0.11345531550889376 1106 14-15 0.12889799799240365 1106 16-17 0.047520510568389795 1106 18-19 0.022499192779861232 1106 20-21 -0.08604050657940832 1106 22-23 -0.07043184768028254 1106 24-25 0.05288756623465929 1106 26-27 0.11804935298169283 1106 28-29 -0.007197202744308129 1106 30-31 0.04042403952183804 1106 32-33 0.03838843801873537 1106 34-35 -0.0298628545794557 1106 36-37 0.0784119160786716 1106 38-39 0.05682203992582657 1106 40-41 0.011951063400026385 1106 42-43 0.086780288385647 1106 44-45 -0.027246417451486593 1106 46-47 -0.0735364269631944 1106 48-49 -0.18322708171480429 1106 50-51 -0.4044832568272625 1106 52-53 -0.14137087712713026 1106 54-55 -0.021901634999764497 1106 56-57 0.04659980553732623 1106 58-59 -0.08194740821358693 1106 60-61 0.05387597051910831 1106 62-63 0.011293382678715602 1106 64-65 -0.11291959967083898 1106 66-67 -0.019698645534596437 1106 68-69 0.12027010551733497 1106 70-71 -0.18799379680518058 1106 72-73 -0.1249517943308831 1106 74-75 -0.23759833757387128 1106 76-77 -0.3734129445090133 1106 78-79 -0.34108097670213766 1106 80-81 -0.3797067814126933 1106 82-83 -0.2796703443592037 1106 84-85 -0.29357419213491553 1106 86-87 -0.3783583247250064 1106 88-89 -0.5971988732696829 1106 90-91 -0.7423122527636892 1106 92-93 -0.8789714896771095 1106 94-95 -0.7774773020597685 1106 96-97 -0.9122488592679971 1106 98-99 -0.8462276619438924 1106 100-101 -0.8705477430841633 1107 1 0.38878691870015913 1107 2 1.4570979352849562 1107 3 0.7110738182293623 1107 4 0.30959088970376314 1107 5 0.21819108796717757 1107 6 0.14456398473225818 1107 7 0.11486978928338942 1107 8 0.15927208771738322 1107 9 0.08401890494741338 1107 10-11 0.20623090905196761 1107 12-13 0.26936872124016986 1107 14-15 0.261260414692174 1107 16-17 0.24535079584613584 1107 18-19 0.2347836145627369 1107 20-21 0.22059627993970565 1107 22-23 0.17765856320082918 1107 24-25 0.14440059851541065 1107 26-27 0.3076533606299634 1107 28-29 0.23391136646737465 1107 30-31 0.17890487315537484 1107 32-33 0.15717331429870285 1107 34-35 0.21794514203772763 1107 36-37 0.2556915431481528 1107 38-39 0.20789074955435893 1107 40-41 0.2236499930311524 1107 42-43 0.31976295591579884 1107 44-45 0.050199510736945285 1107 46-47 0.016759914720097413 1107 48-49 0.01784157659678698 1107 50-51 0.015223442873832482 1107 52-53 0.04780757618693343 1107 54-55 0.08312123354230039 1107 56-57 0.0837653516129393 1107 58-59 0.21702659496327215 1107 60-61 0.2054432071802097 1107 62-63 0.2050267966138648 1107 64-65 0.12138772931286468 1107 66-67 0.11028375959491399 1107 68-69 0.0011074338749779145 1107 70-71 -0.051219901667593604 1107 72-73 -0.03710268941817674 1107 74-75 -0.0012843791248542402 1107 76-77 -0.009877569513840001 1107 78-79 -0.04055087264162438 1107 80-81 0.015027928788530431 1107 82-83 0.07756649121890646 1107 84-85 -0.04110083503841366 1107 86-87 -0.05284247822288535 1107 88-89 0.023046757006767393 1107 90-91 -0.04843590402603226 1107 92-93 -0.19428101126289832 1107 94-95 -0.051727860815791615 1107 96-97 0.04786213586681498 1107 98-99 -0.06693381050098068 1107 100-101 -0.06801778339533371 1108 1 0.28515460784257485 1108 2 1.4221814602927694 1108 3 0.6179168427515584 1108 4 0.207163398123285 1108 5 0.13336939578917395 1108 6 0.08046936715967945 1108 7 0.07830719593776791 1108 8 0.12994424548960382 1108 9 0.10254624405495605 1108 10-11 0.13145841958323246 1108 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-2.8445710167474303 2316 58-59 -2.799036729260429 2316 60-61 -2.803253965375866 2316 62-63 -2.4750300953546542 2316 64-65 -2.24392354979404 2316 66-67 -2.2588775673352828 2316 68-69 -2.262369109961824 2316 70-71 -2.2116346856619487 2316 72-73 -2.3393543427571117 2316 74-75 -2.3378878517054105 2316 76-77 -2.344276051727313 2316 78-79 -2.5666135188757835 2316 80-81 -2.5196961021641933 2316 82-83 -2.7172233866246707 2316 84-85 -2.26440856857851 2316 86-87 -2.169670247036642 2316 88-89 -2.1763660994951515 2316 90-91 -1.9817686230735347 2316 92-93 -2.398161432107848 2316 94-95 -2.257567830711235 2316 96-97 -1.9697860685529491 2316 98-99 -1.9642227168628885 2316 100-101 -2.0742560152713985 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 34253.0 3 10367.0 4 8288.0 5 6471.0 6 5910.0 7 5497.0 8 6068.0 9 6955.0 10 7801.0 11 8793.0 12 9691.0 13 10480.0 14 11418.0 15 12587.0 16 14461.0 17 17055.0 18 16224.0 19 14480.0 20 15177.0 21 16347.0 22 17621.0 23 19481.0 24 22243.0 25 25730.0 26 30117.0 27 35872.0 28 43957.0 29 53163.0 30 65083.0 31 82029.0 32 105506.0 33 141409.0 34 204196.0 35 322908.0 36 608740.0 37 1191501.0 38 1156520.0 39 140598.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.468981616185395 17.86832595567184 13.545808503597026 25.116883924545736 2 17.437036194693174 20.093824931792355 36.94291169120306 25.526227182311413 3 18.905991896845027 23.019183745180406 28.831101793191188 29.24372256478337 4 13.847053247098826 16.149157445500386 35.17439032353933 34.82939898386146 5 15.70485298710114 35.6359853211433 33.459477444429 15.199684247326564 6 34.39022727933689 33.87564805258436 17.249493113154372 14.484631554924379 7 30.356282576871312 30.43609438528923 20.59508749993895 18.612535537900506 8 27.368086868296672 33.4974879503762 19.898829782274234 19.2355953990529 9 26.919084534775255 15.871842149777812 19.296045974827326 37.91302734061961 10-11 25.994355187197126 25.040617810297928 27.125371254611647 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24.773774644109704 25.242222545891174 25.42286534651179 24.561137463487327 38-39 24.560424569382246 25.37840350030869 25.70095750554388 24.360214424765186 40-41 24.747004789118396 25.158383898705345 25.685525473791937 24.40908583838432 42-43 24.797800312490427 25.168521939820504 25.4409076519306 24.592770095758468 44-45 24.663771417406576 25.369688250039413 25.34477667281685 24.621763659737166 46-47 24.68404255909598 25.343641944131473 25.30649986401958 24.665815632752967 48-49 24.791059728539437 25.32687659394972 25.297793515694593 24.58427016181625 50-51 24.55868292939049 25.406345161915883 25.421630455764838 24.61334145292879 52-53 24.89357658811481 25.321767786390815 25.18633393518756 24.59832169030682 54-55 24.69851287143527 25.156834577731544 25.320689776887427 24.823962773945755 56-57 24.597659172496165 25.50753820162108 25.4134078088265 24.48139481705625 58-59 24.727354424651207 25.111654008938096 25.459955685832288 24.701035880578413 60-61 24.805666669774755 25.0950631541697 25.55588190501055 24.54338827104499 62-63 24.67643912914848 25.279916163084557 25.47122463286658 24.572420074900382 64-65 24.638322100052406 25.14597603884954 25.39246549286402 24.823236368234042 66-67 24.583675194329313 25.35651878919098 25.331397487048317 24.728408529431388 68-69 24.381844411596386 25.20056772987426 25.62182462875251 24.795763229776842 70-71 24.3599190703166 25.201153068154596 25.56068760480596 24.878240256722844 72-73 24.583652270061652 25.152848172055425 25.398679699082848 24.86481985880007 74-75 24.46995602291334 25.2656007512979 25.349952993916137 24.914490231872623 76-77 24.62143782448147 25.1303366978616 25.427121270704795 24.821104206952135 78-79 24.460434610127386 25.193759675493 25.50453015422943 24.841275560150187 80-81 24.3478816888231 25.31059953200121 25.579667196668616 24.76185158250707 82-83 24.490191239824664 25.194431835879893 25.46585547066097 24.84952145363447 84-85 24.463198041814486 25.08821393059072 25.401360201360333 25.047227826234458 86-87 24.479914064118415 25.019888659034777 25.668104630221926 24.83209264662488 88-89 24.393443503772545 24.937438818150394 25.65153882412287 25.01757885395419 90-91 24.272885633474832 25.123531425091205 25.750377985234067 24.853204956199896 92-93 24.362811931384478 24.899754881112788 25.793649295435884 24.943783892066847 94-95 24.371834067246954 24.83822947244858 25.722035936071986 25.067900524232478 96-97 24.471033310848224 24.827106823831375 25.659922466358985 25.041937398961416 98-99 24.16305380541387 24.833232902011915 25.700630673147597 25.303082619426615 100-101 24.07593031227604 24.669731533157474 25.99934730517904 25.254990849387447 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 120.0 1 103.5 2 113.5 3 139.5 4 127.0 5 155.0 6 259.0 7 457.0 8 722.0 9 894.0 10 893.5 11 852.0 12 897.5 13 989.0 14 1067.0 15 1096.5 16 1141.0 17 1200.5 18 1287.0 19 1399.5 20 1542.5 21 1775.0 22 2062.0 23 2425.5 24 2962.5 25 3765.5 26 4925.0 27 6730.0 28 9170.5 29 12390.5 30 17054.5 31 22601.5 32 28501.5 33 34968.5 34 42082.5 35 49469.0 36 58082.0 37 67717.5 38 78278.0 39 87132.0 40 94750.0 41 107562.0 42 121332.0 43 135138.0 44 148412.0 45 158900.0 46 168296.0 47 178970.0 48 193611.5 49 205189.0 50 211016.0 51 213607.5 52 213041.0 53 213651.0 54 211550.5 55 201366.5 56 188078.0 57 172910.0 58 156408.0 59 137498.0 60 115926.5 61 94502.0 62 75376.0 63 59202.5 64 45917.0 65 34727.5 66 25514.5 67 19601.5 68 14697.0 69 10626.0 70 7925.0 71 5773.5 72 4307.0 73 3349.5 74 2525.0 75 1893.5 76 1340.5 77 813.5 78 485.5 79 325.0 80 227.5 81 173.5 82 133.0 83 106.0 84 91.0 85 81.5 86 69.5 87 63.5 88 60.0 89 52.5 90 54.0 91 49.0 92 35.5 93 26.5 94 36.5 95 40.0 96 27.0 97 20.5 98 14.5 99 15.0 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05924529616987707 2 0.05706993497667813 3 0.06219757207493276 4 0.06341843805070767 5 0.0608435207199824 6 0.012031079252181854 7 0.014495008766927584 8 0.015738072305898403 9 0.006059934752482741 10-11 0.011309658448314861 12-13 0.014850169778062104 14-15 0.017136518787240575 16-17 0.03157603364617839 18-19 0.03021088350963008 20-21 0.030266377417619846 22-23 0.018579360394974563 24-25 0.007258603165061745 26-27 0.011964486562594131 28-29 0.019700337336367887 30-31 0.017758050556725986 32-33 0.020466153266626697 34-35 0.025937852594417887 36-37 0.0240399609411678 38-39 0.010699225460427404 40-41 0.011465041390686213 42-43 0.012008881688985945 44-45 0.023729195056425095 46-47 0.014961157594041641 48-49 0.014683688054092798 50-51 0.014861268559660058 52-53 0.013007772032801783 54-55 0.009145396036713882 56-57 0.013518315986307657 58-59 0.012663709803265217 60-61 0.013873476997442173 62-63 0.022563822988639952 64-65 0.016637073615332655 66-67 0.018945620187707034 68-69 0.024339628044312552 70-71 0.0186903482109541 72-73 0.02445061586029209 74-75 0.04125417119959405 76-77 0.04967814643244094 78-79 0.03719201713474298 80-81 0.02058823986420419 82-83 0.017436185890385324 84-85 0.02294118156297038 86-87 0.0261043343183872 88-89 0.02237514370147474 90-91 0.026315211168748315 92-93 0.0507436294658445 94-95 0.07904552254062654 96-97 0.09418426064023544 98-99 0.0979134512571479 100-101 0.08126527886021728 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4504999.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.48031574731281 #Duplication Level Percentage of deduplicated Percentage of total 1 76.35950942757341 39.31011655641388 2 11.96712734980578 12.321429891126085 3 4.2157457052392715 6.510837600482867 4 2.109104376145867 4.343094369121137 5 1.2680946474901882 3.2640956425136114 6 0.8418005419150104 2.600169461642626 7 0.5821314633710006 2.097781807854906 8 0.42959354983494785 1.7692489270809661 9 0.32039722004686255 1.484473504731637 >10 1.7442855324217414 16.046976428096237 >50 0.10551703714577708 3.7065572509379847 >100 0.05307248790593552 4.930810283561572 >500 0.002704590510898065 0.9412386234483369 >1k 9.16070593338525E-4 0.6731696529881858 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.219756319590748E-5 2 2.219756319590748E-5 0.0 0.0 0.0 2.219756319590748E-5 3 2.219756319590748E-5 0.0 0.0 0.0 2.219756319590748E-5 4 2.219756319590748E-5 0.0 0.0 0.0 2.219756319590748E-5 5 2.219756319590748E-5 0.0 0.0 0.0 2.219756319590748E-5 6 2.219756319590748E-5 0.0 0.0 0.0 4.439512639181496E-5 7 2.219756319590748E-5 0.0 0.0 0.0 4.439512639181496E-5 8 2.219756319590748E-5 0.0 0.0 0.0 4.439512639181496E-5 9 2.219756319590748E-5 0.0 0.0 2.219756319590748E-5 4.439512639181496E-5 10-11 2.219756319590748E-5 0.0 0.0 2.219756319590748E-5 4.439512639181496E-5 12-13 2.219756319590748E-5 0.0 0.0 2.219756319590748E-5 9.988903438158366E-5 14-15 2.219756319590748E-5 0.0 0.0 2.219756319590748E-5 1.331853791754449E-4 16-17 2.219756319590748E-5 0.0 0.0 2.219756319590748E-5 1.6648172396930612E-4 18-19 2.219756319590748E-5 0.0 0.0 2.219756319590748E-5 1.7758050556725984E-4 20-21 2.219756319590748E-5 0.0 0.0 4.439512639181496E-5 1.7758050556725984E-4 22-23 4.439512639181496E-5 0.0 0.0 8.879025278362992E-5 1.7758050556725984E-4 24-25 6.659268958772244E-5 0.0 0.0 1.5538294237135236E-4 1.7758050556725984E-4 26-27 6.659268958772244E-5 0.0 0.0 2.99667103144751E-4 1.7758050556725984E-4 28-29 8.879025278362992E-5 0.0 0.0 0.001032186688609698 1.7758050556725984E-4 30-31 1.109878159795374E-4 0.0 0.0 0.003318535697788168 1.7758050556725984E-4 32-33 1.109878159795374E-4 0.0 0.0 0.008657049646403916 1.7758050556725984E-4 34-35 1.331853791754449E-4 3.329634479386122E-5 0.0 0.016071035753837015 1.9977806876316732E-4 36-37 1.7758050556725984E-4 4.439512639181496E-5 0.0 0.02774695399488435 1.9977806876316732E-4 38-39 1.7758050556725984E-4 5.5493907989768705E-5 0.0 0.045027756942898324 1.9977806876316732E-4 40-41 1.7758050556725984E-4 6.659268958772244E-5 0.0 0.06531632970395776 2.4417319515498227E-4 42-43 2.1087685036112107E-4 6.659268958772244E-5 0.0 0.08733631239429798 2.55271976752936E-4 44-45 2.219756319590748E-4 6.659268958772244E-5 0.0 0.11436184558531534 2.7746953994884347E-4 46-47 2.4417319515498227E-4 7.769147118567618E-5 0.0 0.146348534150618 2.99667103144751E-4 48-49 2.663707583508898E-4 8.879025278362992E-5 0.0 0.1801998180243769 3.1076588474270473E-4 50-51 2.663707583508898E-4 1.109878159795374E-4 0.0 0.2142730775300949 3.1076588474270473E-4 52-53 2.663707583508898E-4 1.109878159795374E-4 0.0 0.25127641537767265 3.329634479386122E-4 54-55 2.663707583508898E-4 1.109878159795374E-4 0.0 0.2963707650101587 3.329634479386122E-4 56-57 2.663707583508898E-4 1.109878159795374E-4 0.0 0.35108775828807065 3.329634479386122E-4 58-59 2.663707583508898E-4 1.109878159795374E-4 0.0 0.4093896580221217 3.329634479386122E-4 60-61 2.885683215467972E-4 1.109878159795374E-4 0.0 0.47354061565829425 3.329634479386122E-4 62-63 3.1076588474270473E-4 1.109878159795374E-4 0.0 0.5432742604382377 3.7735857433042713E-4 64-65 3.1076588474270473E-4 1.109878159795374E-4 0.0 0.6222087951628846 3.7735857433042713E-4 66-67 3.1076588474270473E-4 1.331853791754449E-4 0.0 0.7174918351813175 3.7735857433042713E-4 68-69 3.1076588474270473E-4 1.7758050556725984E-4 0.0 0.8240623360848693 3.7735857433042713E-4 70-71 3.1076588474270473E-4 1.7758050556725984E-4 0.0 0.9419535942183339 3.7735857433042713E-4 72-73 3.218646663406585E-4 1.7758050556725984E-4 0.0 1.067935864136707 4.5505004551610334E-4 74-75 3.329634479386122E-4 1.7758050556725984E-4 0.0 1.2128637542427867 4.772476087120108E-4 76-77 3.329634479386122E-4 1.9977806876316732E-4 0.0 1.3835075213113255 4.883463903099645E-4 78-79 3.329634479386122E-4 1.9977806876316732E-4 0.0 1.5751834795079867 4.883463903099645E-4 80-81 3.7735857433042713E-4 1.9977806876316732E-4 0.0 1.7859826383979218 4.883463903099645E-4 82-83 3.7735857433042713E-4 2.1087685036112107E-4 0.0 2.012530968375354 4.883463903099645E-4 84-85 3.9955613752633463E-4 2.4417319515498227E-4 0.0 2.2639960630401914 4.883463903099645E-4 86-87 4.2175370072224214E-4 2.4417319515498227E-4 0.0 2.5452502875139373 4.994451719079183E-4 88-89 4.2175370072224214E-4 2.4417319515498227E-4 0.0 2.8588130652193264 5.10543953505872E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3375 0.0 41.530144 1 GTATCAA 5640 0.0 37.90955 1 TCAACGC 6655 0.0 32.554626 4 ATCAACG 6700 0.0 32.336693 3 CAACGCA 6960 0.0 31.262117 5 AACGCAG 7350 0.0 29.527525 6 TATCAAC 7280 0.0 28.6522 2 GTACATG 11700 0.0 25.502867 1 ACGCAGA 8705 0.0 24.877337 7 ACATGGG 12725 0.0 23.8588 3 TACATGG 12355 0.0 23.497591 2 CGCAGAG 9260 0.0 23.334764 8 CATGGGG 10810 0.0 19.821968 4 TTATAGG 2015 0.0 18.863424 3 GCTTATA 2135 0.0 18.693758 1 GCAGAGT 11590 0.0 18.234509 9 AGAGTAC 9915 0.0 16.813602 10-11 TATAGGA 2475 0.0 16.700903 4 GCGTAAA 200 2.7566837E-4 16.629656 1 TATAAGC 1805 0.0 16.583221 3 >>END_MODULE