FastQCFastQC Report
Fri 27 May 2016
SRR940215_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940215_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4504999
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38200.041.7926751
GTATCAA60950.038.7442251
TCAACGC70450.032.6352844
ATCAACG71200.032.2915153
CAACGCA74200.030.9859285
TATCAAC77100.030.2520622
AACGCAG77850.029.5908856
GTACATG117950.025.096571
ACGCAGA92100.024.9609187
CGCAGAG96700.023.7735318
TACATGG124250.023.7423152
ACATGGG126950.022.301633
CATGGGG107750.019.838934
GCAGAGT122650.018.5886789
AGAGTAC101000.017.49429310-11
CAGAGTA107850.017.17589210-11
ATGGGGA68000.016.83575
TATAAGC19800.016.7940673
GCTTATA18850.016.384241
GTGGTAT23550.015.7372181
Produced by FastQC (version 0.11.5)