FastQCFastQC Report
Fri 27 May 2016
SRR940214_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940214_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591040
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58100.040.3995671
GGTATCA35100.038.0173341
ATCAACG68050.036.012283
TCAACGC70700.034.7296334
CAACGCA72500.033.8011325
AACGCAG76250.032.1989636
TATCAAC73450.031.8252332
ACGCAGA88500.027.6353287
GTACATG118750.026.6780621
CGCAGAG94400.025.9075578
TACATGG126000.024.87582
ACATGGG129600.024.8091963
CATGGGG109850.021.4010074
GCAGAGT119350.020.2513269
AGAGTAC103750.017.6904310-11
TATAAGC20550.017.3331893
ATGGGGA70100.017.2759425
CAGAGTA107050.017.23381210-11
GCTTATA18900.017.0159821
TTATAGG20200.015.5174943
Produced by FastQC (version 0.11.5)