##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR940214_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4591040 Sequences flagged as poor quality 0 Sequence length 101 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.376712901651914 33.0 31.0 34.0 28.0 34.0 2 29.580747281661672 33.0 30.0 34.0 16.0 34.0 3 30.95296839060431 33.0 31.0 34.0 25.0 34.0 4 35.026802641667246 37.0 35.0 37.0 32.0 37.0 5 35.33275401651913 37.0 35.0 37.0 33.0 37.0 6 35.565448133756185 37.0 35.0 37.0 35.0 37.0 7 35.61935683418136 37.0 35.0 37.0 35.0 37.0 8 35.652230213633516 37.0 36.0 37.0 35.0 37.0 9 37.40519707952882 39.0 38.0 39.0 35.0 39.0 10-11 37.332822083885134 39.0 38.0 39.0 35.0 39.0 12-13 37.25323935317488 39.0 38.0 39.0 35.0 39.0 14-15 38.64045597511675 41.0 39.0 41.0 35.0 41.0 16-17 38.568926866243814 41.0 39.0 41.0 35.0 41.0 18-19 38.49601778681955 40.5 39.0 41.0 34.0 41.0 20-21 38.39816762650729 40.0 39.0 41.0 34.0 41.0 22-23 38.285528333449506 40.0 38.0 41.0 34.0 41.0 24-25 38.16669273193002 40.0 38.0 41.0 34.0 41.0 26-27 38.0212192662229 40.0 38.0 41.0 33.5 41.0 28-29 37.84930190109431 40.0 38.0 41.0 33.0 41.0 30-31 37.688293284310305 40.0 38.0 41.0 33.0 41.0 32-33 37.771070389279984 40.0 38.0 41.0 33.0 41.0 34-35 37.82187892068028 40.0 38.0 41.0 33.0 41.0 36-37 37.92855333432077 40.0 38.0 41.0 33.0 41.0 38-39 37.85914836289817 40.0 38.0 41.0 33.0 41.0 40-41 37.72773140726284 40.0 38.0 41.0 33.0 41.0 42-43 37.483567666585344 40.0 38.0 41.0 32.0 41.0 44-45 37.43299154439953 40.0 38.0 41.0 32.0 41.0 46-47 37.25636892730188 40.0 37.5 41.0 32.0 41.0 48-49 37.06076074266397 40.0 37.0 41.0 31.0 41.0 50-51 36.14468181501359 39.0 36.0 40.0 30.0 40.5 52-53 36.2488277165958 39.0 36.0 40.0 30.5 41.0 54-55 36.558024543458565 40.0 36.0 41.0 31.0 41.0 56-57 36.39641911200948 39.0 35.5 41.0 30.5 41.0 58-59 36.13206060064822 39.0 35.0 41.0 30.0 41.0 60-61 35.81489433766641 39.0 35.0 41.0 29.5 41.0 62-63 35.46478052903046 38.0 35.0 40.0 29.0 41.0 64-65 35.07187935631143 37.0 35.0 40.0 29.0 41.0 66-67 34.69444363368649 37.0 34.5 39.5 28.0 41.0 68-69 34.305389083954836 36.0 34.0 39.0 28.0 41.0 70-71 33.883667643932526 36.0 34.0 39.0 27.5 40.5 72-73 33.45729006935248 35.0 34.0 37.5 26.5 40.0 74-75 33.02307614396738 35.0 33.5 37.0 26.0 39.0 76-77 32.559466809263256 35.0 33.0 36.5 25.5 39.0 78-79 32.129271798982366 35.0 33.0 36.0 25.0 37.5 80-81 31.791112035617203 35.0 33.0 35.5 24.0 37.0 82-83 31.452043001150066 35.0 32.0 35.0 23.0 37.0 84-85 31.165743273855163 35.0 32.0 35.0 21.5 36.0 86-87 30.889549208893847 35.0 32.0 35.0 20.0 36.0 88-89 30.60439638948909 34.0 31.5 35.0 19.0 35.5 90-91 30.358529875583745 34.0 31.0 35.0 18.0 35.0 92-93 30.088145823168606 34.0 31.0 35.0 12.5 35.0 94-95 29.759628755140447 34.0 31.0 35.0 4.5 35.0 96-97 29.39650678277689 34.0 30.5 35.0 2.0 35.0 98-99 28.938378885829792 34.0 30.0 35.0 2.0 35.0 100-101 27.037134832194884 32.5 27.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.7223892826147811 1101 2 1.8231358147750214 1101 3 0.9917805346291715 1101 4 0.5754760803767667 1101 5 0.4650653140373038 1101 6 0.387493407261168 1101 7 0.37534376014130544 1101 8 0.3616189532392369 1101 9 0.4391140445050894 1101 10-11 0.47802461129416685 1101 12-13 0.521241567249298 1101 14-15 0.639612998302276 1101 16-17 0.566284677587106 1101 18-19 0.5615248470400331 1101 20-21 0.4707795199085396 1101 22-23 0.5079477040706095 1101 24-25 0.5178733204885404 1101 26-27 0.5639090049703199 1101 28-29 0.6746268414260115 1101 30-31 0.6731327207373852 1101 32-33 0.6705900281155479 1101 34-35 0.742613244507595 1101 36-37 0.778618726084467 1101 38-39 0.7563807231325796 1101 40-41 0.7929451278189603 1101 42-43 0.8294670123389309 1101 44-45 0.7413905839649075 1101 46-47 0.6405234984411479 1101 48-49 0.6696934646052668 1101 50-51 0.6458665809093773 1101 52-53 0.7077097679963558 1101 54-55 0.826959413717212 1101 56-57 0.837974011588102 1101 58-59 0.7716678398392318 1101 60-61 0.8222597388160438 1101 62-63 0.9211750882434941 1101 64-65 0.9103120869267514 1101 66-67 0.8846669434092718 1101 68-69 0.917672151863691 1101 70-71 0.7762807329763319 1101 72-73 0.7153716965552661 1101 74-75 0.7643485135147827 1101 76-77 0.8493316489038705 1101 78-79 0.7799925802140777 1101 80-81 0.7066242082301208 1101 82-83 0.7042103646934379 1101 84-85 0.7772015896575688 1101 86-87 0.6610284179725525 1101 88-89 0.7700199457160863 1101 90-91 0.6262081147099146 1101 92-93 0.5713039940631681 1101 94-95 0.6170554416458991 1101 96-97 0.4885143794853164 1101 98-99 0.418595618304888 1101 100-101 0.3492858397656633 1102 1 0.9781783446451975 1102 2 2.754784541137731 1102 3 1.5500478851049522 1102 4 0.8073143440409964 1102 5 0.5929133305361134 1102 6 0.4315826842061057 1102 7 0.3235736645608327 1102 8 0.3635610278265773 1102 9 0.3924022467028152 1102 10-11 0.3212781732710752 1102 12-13 0.4026898135522643 1102 14-15 0.44780250243258735 1102 16-17 0.4332640411039179 1102 18-19 0.4673848281199753 1102 20-21 0.47441959257593425 1102 22-23 0.44152528641814115 1102 24-25 0.5150144235834162 1102 26-27 0.5379731092067246 1102 28-29 0.49186700422932006 1102 30-31 0.4757110528041437 1102 32-33 0.5718545807369324 1102 34-35 0.5519579422642451 1102 36-37 0.5487746320188975 1102 38-39 0.570850497550893 1102 40-41 0.5878080671223813 1102 42-43 0.7737974913686188 1102 44-45 0.5990858433001662 1102 46-47 0.5288149279731513 1102 48-49 0.65469078721474 1102 50-51 0.5910766893493005 1102 52-53 0.6095565314714122 1102 54-55 0.7538696451899156 1102 56-57 0.7760878192664222 1102 58-59 0.7435817700265517 1102 60-61 0.7550927459658041 1102 62-63 0.8346754527526912 1102 64-65 0.7906595728109096 1102 66-67 0.6863759341290603 1102 68-69 0.6892618085316897 1102 70-71 0.714018091066535 1102 72-73 0.7795802165182266 1102 74-75 0.6822765486992068 1102 76-77 0.772022028579542 1102 78-79 0.8003631637355682 1102 80-81 0.833232700159666 1102 82-83 0.8995365100167589 1102 84-85 0.7508077815502752 1102 86-87 0.6666789029082096 1102 88-89 0.6534625174974629 1102 90-91 0.6054490788598201 1102 92-93 0.5767007379472524 1102 94-95 0.43193196867506956 1102 96-97 0.45445138137158736 1102 98-99 0.3929921516889685 1102 100-101 0.5308143204918174 1103 1 0.7588611507856164 1103 2 2.586402294207403 1103 3 1.3105778568361544 1103 4 0.6764073908524111 1103 5 0.4165651991578443 1103 6 0.3587787877744688 1103 7 0.24676479283945696 1103 8 0.2679035416612763 1103 9 0.22081159573660614 1103 10-11 0.20917152796091898 1103 12-13 0.19106645737942074 1103 14-15 0.2845594036242787 1103 16-17 0.302026248109982 1103 18-19 0.2993242811406631 1103 20-21 0.4049766698487929 1103 22-23 0.2779013221441389 1103 24-25 0.3121619753287419 1103 26-27 0.40435245017911114 1103 28-29 0.347348372564646 1103 30-31 0.33897250008489266 1103 32-33 0.36518691661321867 1103 34-35 0.31044038606531643 1103 36-37 0.37653694239987345 1103 38-39 0.38865157008271467 1103 40-41 0.40162109744881036 1103 42-43 0.46552573410297526 1103 44-45 0.3189352689276532 1103 46-47 0.30284555330422336 1103 48-49 0.39593990675165713 1103 50-51 0.3070943957971153 1103 52-53 0.2944159587927757 1103 54-55 0.44889740398760836 1103 56-57 0.49471995382524625 1103 58-59 0.4984336000947138 1103 60-61 0.5566704911138913 1103 62-63 0.49943370446531077 1103 64-65 0.44350438255409586 1103 66-67 0.45472400545362035 1103 68-69 0.4067921751962942 1103 70-71 0.29785184336238046 1103 72-73 0.36120304091772937 1103 74-75 0.3877589542217663 1103 76-77 0.5501393721608281 1103 78-79 0.5775445461216364 1103 80-81 0.4917188342843666 1103 82-83 0.4967244203790244 1103 84-85 0.45705561405817363 1103 86-87 0.39414297325265224 1103 88-89 0.41850663176363767 1103 90-91 0.4403871776921875 1103 92-93 0.34120095925206684 1103 94-95 0.366121980458896 1103 96-97 0.34003556189396633 1103 98-99 0.21778684592044684 1103 100-101 0.18627765552208686 1104 1 0.8388086465902695 1104 2 2.6285739629879004 1104 3 1.3699397757869605 1104 4 0.7610748919739336 1104 5 0.5234760361788418 1104 6 0.33173242568382477 1104 7 0.24286547018788696 1104 8 0.20133348345251534 1104 9 0.21405007444415958 1104 10-11 0.23532925100682434 1104 12-13 0.3185858003016051 1104 14-15 0.29262176066389145 1104 16-17 0.3239037438592476 1104 18-19 0.28875141495436196 1104 20-21 0.3625397662670977 1104 22-23 0.3295014262663827 1104 24-25 0.4186788370400052 1104 26-27 0.3731324753235228 1104 28-29 0.3589894609743993 1104 30-31 0.23907889307989905 1104 32-33 0.40098198409883423 1104 34-35 0.34283658228035563 1104 36-37 0.3634011736398648 1104 38-39 0.3408901152512769 1104 40-41 0.3387595634180087 1104 42-43 0.4520972876843885 1104 44-45 0.3253123132959175 1104 46-47 0.31647994576754 1104 48-49 0.310505971761593 1104 50-51 0.17406155852135896 1104 52-53 0.25757363199293337 1104 54-55 0.3325105566208535 1104 56-57 0.4093746335108861 1104 58-59 0.4657407108685945 1104 60-61 0.4043939524031259 1104 62-63 0.5226963583640796 1104 64-65 0.5321794823840662 1104 66-67 0.45645852696206646 1104 68-69 0.4398434277093486 1104 70-71 0.3540861350265061 1104 72-73 0.4059578828789938 1104 74-75 0.38159331123921447 1104 76-77 0.5408528587606227 1104 78-79 0.6376800158070992 1104 80-81 0.5257507743365402 1104 82-83 0.6210954777182565 1104 84-85 0.4956833327287349 1104 86-87 0.5042749814273577 1104 88-89 0.5449796292104239 1104 90-91 0.5040555206531359 1104 92-93 0.20103630210778434 1104 94-95 0.19051009612320158 1104 96-97 0.2620786840105289 1104 98-99 0.1345717116415166 1104 100-101 0.009780455191997106 1105 1 0.752787951243544 1105 2 2.428463079944116 1105 3 1.242546796969009 1105 4 0.7193443546990395 1105 5 0.4598452513215747 1105 6 0.3071680225266178 1105 7 0.3668534508045127 1105 8 0.26476726045909516 1105 9 0.2385827362720363 1105 10-11 0.1960581377325994 1105 12-13 0.18675591266590885 1105 14-15 0.024926710195181556 1105 16-17 0.03079616191371315 1105 18-19 0.05893636109991007 1105 20-21 0.1974696179292792 1105 22-23 0.17355341856382012 1105 24-25 0.17013914979789746 1105 26-27 0.17172548799889142 1105 28-29 0.25188617468877084 1105 30-31 0.3212834342060873 1105 32-33 0.3666103566150767 1105 34-35 0.26506006239971214 1105 36-37 0.3661442593203006 1105 38-39 0.2019432925583402 1105 40-41 0.22675689714634473 1105 42-43 0.32948793880485994 1105 44-45 0.15329187187982285 1105 46-47 -0.00441240880427074 1105 48-49 0.14214350694157218 1105 50-51 0.08995253763556121 1105 52-53 0.2115331512176013 1105 54-55 0.2248113797793252 1105 56-57 0.22692695380206374 1105 58-59 0.30548783827828885 1105 60-61 0.24595382713067693 1105 62-63 0.30646415995961007 1105 64-65 0.4435014029460973 1105 66-67 0.18719302132268467 1105 68-69 0.18148995626596331 1105 70-71 0.1756470677983728 1105 72-73 0.13388866562017654 1105 74-75 0.09346932960686871 1105 76-77 -0.022005659554125145 1105 78-79 -0.024150612459607146 1105 80-81 0.14193292724510442 1105 82-83 0.17827610704301478 1105 84-85 0.173072290978304 1105 86-87 0.159233929308094 1105 88-89 0.07298161833372774 1105 90-91 -0.1694190968638445 1105 92-93 -0.12900030622537528 1105 94-95 -0.3844215542143168 1105 96-97 -0.43180984044087367 1105 98-99 -0.35356073434531865 1105 100-101 -0.0986388266075231 1106 1 0.7030604671211584 1106 2 2.530426858871806 1106 3 1.264145658907573 1106 4 0.773410391673778 1106 5 0.4123257738657884 1106 6 0.3548392040619177 1106 7 0.22038354499278512 1106 8 0.1766737073973914 1106 9 0.08939160342909958 1106 10-11 0.0846300105401454 1106 12-13 0.12202353684011058 1106 14-15 0.13389665430312192 1106 16-17 0.133623290749334 1106 18-19 0.049249026731040146 1106 20-21 0.1357452056740911 1106 22-23 0.20407037153300678 1106 24-25 0.1796637507768608 1106 26-27 0.12398897311800994 1106 28-29 0.12417830027176535 1106 30-31 0.136709201330369 1106 32-33 0.17167469936519808 1106 34-35 0.1446443237780244 1106 36-37 0.1349642201253971 1106 38-39 0.21127314451027246 1106 40-41 0.28335043335771104 1106 42-43 0.15406006794174942 1106 44-45 0.2114741904655446 1106 46-47 0.23807405220661337 1106 48-49 0.007456223634328296 1106 50-51 -0.02228198022528005 1106 52-53 0.09236234600645332 1106 54-55 0.2340489378491526 1106 56-57 0.3487517378879943 1106 58-59 0.196497112090114 1106 60-61 0.2857411452619445 1106 62-63 0.2722666086707619 1106 64-65 0.2649388590969295 1106 66-67 0.19904146225817954 1106 68-69 0.2745518817588888 1106 70-71 0.18549440376035875 1106 72-73 0.17749495906723212 1106 74-75 0.12307661977679629 1106 76-77 0.18042441410737808 1106 78-79 0.3018561440142449 1106 80-81 0.3472018483845112 1106 82-83 0.2939144947620811 1106 84-85 0.06723750494181857 1106 86-87 0.1178749344459149 1106 88-89 0.27480331829767835 1106 90-91 0.00900984425087259 1106 92-93 0.0857670723424313 1106 94-95 0.05014246633696473 1106 96-97 0.13228827344492444 1106 98-99 0.07367527962105314 1106 100-101 -0.041738741947352054 1107 1 0.7817780015514195 1107 2 2.5944521926105963 1107 3 1.362221325499739 1107 4 0.7693470280166537 1107 5 0.5403928061765626 1107 6 0.33687476948522743 1107 7 0.23698066111837335 1107 8 0.15746603400037884 1107 9 0.19881713672115353 1107 10-11 0.19427451507039706 1107 12-13 0.2224370541923335 1107 14-15 0.22762403816471277 1107 16-17 0.2785299806687149 1107 18-19 0.2960243607751494 1107 20-21 0.2792738300877389 1107 22-23 0.3124743240065939 1107 24-25 0.2814299315056772 1107 26-27 0.33836731096388206 1107 28-29 0.34086359109561215 1107 30-31 0.3386130176338895 1107 32-33 0.35564326911541144 1107 34-35 0.35367343730477785 1107 36-37 0.35432039827736617 1107 38-39 0.324749340394888 1107 40-41 0.4172318664774508 1107 42-43 0.47672719380074824 1107 44-45 0.40088687614228036 1107 46-47 0.3233836833994488 1107 48-49 0.3761586022750478 1107 50-51 0.359080917278348 1107 52-53 0.3617930818499602 1107 54-55 0.5204311983699696 1107 56-57 0.5362135888971764 1107 58-59 0.5771568102688533 1107 60-61 0.5841594963462384 1107 62-63 0.6362896220227512 1107 64-65 0.5184383076997534 1107 66-67 0.539372715792183 1107 68-69 0.5406573811977253 1107 70-71 0.4840556418210582 1107 72-73 0.5143517386878784 1107 74-75 0.5072746635685945 1107 76-77 0.5075746493823772 1107 78-79 0.5610881692600529 1107 80-81 0.5466732886974448 1107 82-83 0.632240934104118 1107 84-85 0.546468943984646 1107 86-87 0.5756441390051066 1107 88-89 0.52426993464859 1107 90-91 0.5569020170977055 1107 92-93 0.4914786433786311 1107 94-95 0.42180687978592957 1107 96-97 0.5748291458370822 1107 98-99 0.6700849084935037 1107 100-101 0.716737081454113 1108 1 0.8159224771599902 1108 2 2.595372549176581 1108 3 1.3677396306540928 1108 4 0.7671093091963712 1108 5 0.4779591114159132 1108 6 0.36661116075710254 1108 7 0.29472554038480325 1108 8 0.24224915335916108 1108 9 0.26348785736686153 1108 10-11 0.27260499851512776 1108 12-13 0.2664915429227861 1108 14-15 0.35080752579547436 1108 16-17 0.34618452658941834 1108 18-19 0.2991819511067604 1108 20-21 0.25705640964997656 1108 22-23 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-1.2863460872098358 2316 58-59 -1.1868635212705172 2316 60-61 -1.2415698020489998 2316 62-63 -1.0654735557360695 2316 64-65 -1.2771186529605814 2316 66-67 -1.0031622566122067 2316 68-69 -1.0468200962797525 2316 70-71 -1.0106917824258232 2316 72-73 -0.970534888962618 2316 74-75 -1.0740153206484848 2316 76-77 -1.1533824230328023 2316 78-79 -1.2079830891583256 2316 80-81 -1.449412644896647 2316 82-83 -1.7515160873351654 2316 84-85 -1.3406261162551338 2316 86-87 -1.3273120707410904 2316 88-89 -1.5309946008335729 2316 90-91 -1.3248728262984635 2316 92-93 -0.9759215860128947 2316 94-95 -0.8388037526092553 2316 96-97 -1.0808481963582146 2316 98-99 -1.2017398324607242 2316 100-101 -1.6052907788327246 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 36780.0 3 10212.0 4 8613.0 5 6939.0 6 6329.0 7 6361.0 8 6608.0 9 7402.0 10 8387.0 11 9384.0 12 10519.0 13 11155.0 14 11952.0 15 13410.0 16 15452.0 17 17498.0 18 16987.0 19 15199.0 20 16061.0 21 17219.0 22 18316.0 23 20324.0 24 23200.0 25 26795.0 26 31805.0 27 37587.0 28 46165.0 29 55345.0 30 68391.0 31 85663.0 32 109976.0 33 147951.0 34 213911.0 35 340791.0 36 638677.0 37 1212375.0 38 1128884.0 39 132414.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.45571185216788 17.922605504571166 13.555258156691627 25.066424486569332 2 17.779438219988332 20.55704532897126 36.50786100469086 25.155655446349552 3 18.93444739178509 23.048090068793474 28.77037795558654 29.247084583834898 4 13.8610979355399 16.176292120461298 35.1869818723265 34.7756280716723 5 15.64929214578692 35.66881465059401 33.49752428057064 15.18436892304843 6 34.39148379237616 33.886679734250826 17.273522196086084 14.448314277286933 7 30.317569141127006 30.47368390094316 20.626079879081864 18.582667078847976 8 27.329495112030184 33.52463439624047 19.875006181719236 19.270864310010115 9 26.902337707489977 15.871132766353638 19.27405657263889 37.9524729535175 10-11 25.983542018000723 25.051069716548508 27.1437218095437 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24.78680027756324 25.256784122685566 25.426252384287828 24.530163215463364 38-39 24.51805966488226 25.37932637491379 25.728459999834442 24.37415396036951 40-41 24.76282292027016 25.131882437720733 25.698667582190165 24.40662705981895 42-43 24.805804627315997 25.200202285371592 25.4370369353788 24.55695615193361 44-45 24.673302714358602 25.357033773388505 25.351848793795106 24.617814718457787 46-47 24.690434462468836 25.341480071932963 25.31638413771394 24.65170132788426 48-49 24.799609457370554 25.326258197299833 25.302393881673073 24.57173846365653 50-51 24.56322978635654 25.395925217327083 25.436416176470427 24.604428819845953 52-53 24.854801139566383 25.321520126221518 25.222290057876943 24.601388676335155 54-55 24.713865388964 25.17778709206021 25.294129692862505 24.81421782611329 56-57 24.605267844684167 25.52593101409436 25.420455606269005 24.448345534952466 58-59 24.72987363602417 25.135520690951825 25.449927386791586 24.684678286232423 60-61 24.793944941896765 25.094209980310563 25.582252316744214 24.52959276104846 62-63 24.699868234834803 25.283181815012863 25.45916282061114 24.557787129541193 64-65 24.649179430726594 25.184900680084187 25.368129307713637 24.797790581475574 66-67 24.572922138969098 25.40392603921508 25.31606784360631 24.707083978209514 68-69 24.346190511876276 25.19286203513126 25.633658422666095 24.827289030326373 70-71 24.335691757533443 25.229024575284853 25.544114114980943 24.89116955220076 72-73 24.5674186029336 25.191244735091757 25.434629300133484 24.80670736184116 74-75 24.48501529931491 25.248346968068837 25.3546164190278 24.912021313588458 76-77 24.604220292161337 25.139929656350564 25.43793208363894 24.81791796784916 78-79 24.456929524367258 25.196426501717205 25.49057605949614 24.8560679144194 80-81 24.3419111283604 25.31765196953643 25.576845214630417 24.763591687472747 82-83 24.460973855780566 25.227334171483196 25.446504333406168 24.86518763933007 84-85 24.429755144855474 25.08125347882965 25.41570040073939 25.073290975575485 86-87 24.498473243609233 25.000852404035374 25.68080393737679 24.819870414978606 88-89 24.375717801297895 24.970558096057896 25.6271743735228 25.02654972912141 90-91 24.261906330998805 25.150460687308318 25.744907168116963 24.84272581357591 92-93 24.34451342579665 24.95311046363088 25.782135988208076 24.920240122364394 94-95 24.31821894298614 24.85717302683993 25.72752208509782 25.097085945076113 96-97 24.44859155896654 24.85335119574005 25.621128065042292 25.076929180251113 98-99 24.124853455571042 24.84716653158493 25.692459212799733 25.335520800044296 100-101 24.052788346369848 24.713808823705712 26.02759955577401 25.20580327415043 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 184.0 1 137.0 2 122.0 3 152.0 4 143.5 5 167.0 6 243.0 7 429.5 8 754.5 9 944.5 10 892.0 11 879.0 12 940.5 13 1005.5 14 1070.0 15 1134.0 16 1198.0 17 1237.0 18 1308.0 19 1367.5 20 1501.5 21 1736.0 22 2066.5 23 2524.5 24 3008.5 25 3792.5 26 5091.0 27 7032.5 28 9564.0 29 12721.5 30 17351.5 31 23065.5 32 29223.5 33 35863.0 34 43062.0 35 50291.0 36 58853.0 37 69314.5 38 80317.5 39 88985.5 40 96943.5 41 110099.5 42 124001.5 43 137534.0 44 150801.0 45 162169.5 46 172206.0 47 182350.0 48 197208.0 49 209540.0 50 215354.5 51 217835.5 52 216981.5 53 217642.0 54 215864.0 55 205189.0 56 191045.0 57 175637.5 58 158535.5 59 139374.5 60 117518.5 61 95890.5 62 76714.5 63 60135.0 64 46742.5 65 35331.5 66 26131.5 67 20309.5 68 15203.0 69 10863.0 70 7994.0 71 5858.0 72 4436.5 73 3372.0 74 2550.5 75 1923.5 76 1313.0 77 807.5 78 498.5 79 328.0 80 212.0 81 164.0 82 138.5 83 112.0 84 84.5 85 66.5 86 52.0 87 46.5 88 47.5 89 42.0 90 40.0 91 32.0 92 32.0 93 34.5 94 26.0 95 21.5 96 18.5 97 17.5 98 13.5 99 11.5 100 10.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.035242559420087825 2 1.090842162124486 3 0.0264645919007458 4 0.02868631072698125 5 0.031909981180734646 6 0.011892730187495643 7 0.01801334773820311 8 0.019450930508120162 9 0.011261065031016936 10-11 0.01092345089565763 12-13 0.025070572245068656 14-15 0.015247089983968775 16-17 0.026813096814665083 18-19 0.03087535721753677 20-21 0.04409676238934969 22-23 0.03160503938105527 24-25 0.009660120582700216 26-27 0.01498571129852931 28-29 0.023230030668432423 30-31 0.017545044260124067 32-33 0.028555621384261518 34-35 0.024678504216909456 36-37 0.023850805046351155 38-39 0.011631351502056178 40-41 0.012807555586533771 42-43 0.010792761552937897 44-45 0.018688576008921726 46-47 0.014266919913570781 48-49 0.010977904788457518 50-51 0.02453692409562975 52-53 0.015192636091168886 54-55 0.015639158012127972 56-57 0.018960845472921168 58-59 0.02104098417787691 60-61 0.016597546525406008 62-63 0.023001324318672894 64-65 0.019015299365721057 66-67 0.014996602077089286 68-69 0.01550846866940824 70-71 0.01649952951836621 72-73 0.021324144420436328 74-75 0.028141771798982362 76-77 0.024928992123788945 78-79 0.02239144071931414 80-81 0.018198490973722732 82-83 0.016793580539485604 84-85 0.01688070676796543 86-87 0.0235349724681118 88-89 0.023273593782672335 90-91 0.01933113194396041 92-93 0.03913056736599986 94-95 0.058995347459399175 96-97 0.07359988150832927 98-99 0.08046107200111521 100-101 0.07155241513905346 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 4591040.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.229041158206314 #Duplication Level Percentage of deduplicated Percentage of total 1 76.68926813967863 40.054069420599944 2 11.827530176889184 12.354811208173446 3 4.146891852295384 6.4976455569649785 4 2.122579575084165 4.4344118395455565 5 1.2503088889165834 3.265121720984771 6 0.8060166036732439 2.5258484620468504 7 0.5644871679462885 2.0637836469562214 8 0.4037945000524992 1.6871839650159455 9 0.31599521594333585 1.48537144253707 >10 1.724615773929767 15.992386836144409 >50 0.09434086065485137 3.3902748472149975 >100 0.0509163470388731 4.766711622677335 >500 0.0024517415517893828 0.8862750537510958 >1k 8.031563453192583E-4 0.5961043773873348 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 2.178155711995539E-5 2.178155711995539E-5 0.0 0.0 2.178155711995539E-5 6 4.356311423991078E-5 2.178155711995539E-5 0.0 0.0 2.178155711995539E-5 7 4.356311423991078E-5 4.356311423991078E-5 0.0 0.0 2.178155711995539E-5 8 6.534467135986617E-5 4.356311423991078E-5 0.0 0.0 2.178155711995539E-5 9 6.534467135986617E-5 4.356311423991078E-5 0.0 0.0 2.178155711995539E-5 10-11 1.0890778559977696E-4 4.356311423991078E-5 0.0 0.0 2.178155711995539E-5 12-13 1.3068934271973234E-4 4.356311423991078E-5 0.0 2.178155711995539E-5 1.7425245695964315E-4 14-15 1.7425245695964312E-4 4.356311423991078E-5 0.0 2.178155711995539E-5 1.9603401407959853E-4 16-17 1.7425245695964312E-4 4.356311423991078E-5 0.0 3.2672335679933084E-5 1.9603401407959853E-4 18-19 1.7425245695964312E-4 4.356311423991078E-5 0.0 4.356311423991078E-5 1.9603401407959853E-4 20-21 1.7425245695964312E-4 4.356311423991078E-5 0.0 5.445389279988848E-5 1.9603401407959853E-4 22-23 1.7425245695964312E-4 4.356311423991078E-5 0.0 1.3068934271973234E-4 1.9603401407959853E-4 24-25 1.7425245695964312E-4 5.445389279988848E-5 0.0 1.7425245695964312E-4 2.0692479263957622E-4 26-27 1.8514323551962081E-4 6.534467135986617E-5 0.0 3.049417996793755E-4 2.178155711995539E-4 28-29 2.3959712831950932E-4 6.534467135986617E-5 0.0 7.950268348783718E-4 2.178155711995539E-4 30-31 2.3959712831950932E-4 8.712622847982156E-5 0.0 0.0029949641039938666 2.178155711995539E-4 32-33 2.3959712831950932E-4 1.0890778559977696E-4 0.0 0.008048285355823517 2.3959712831950932E-4 34-35 2.3959712831950932E-4 1.0890778559977696E-4 0.0 0.015421342440928418 2.3959712831950932E-4 36-37 2.3959712831950932E-4 1.1979856415975465E-4 0.0 0.026802206036105107 2.3959712831950932E-4 38-39 2.3959712831950932E-4 1.4158012127971005E-4 0.0 0.044434376524708996 2.3959712831950932E-4 40-41 2.3959712831950932E-4 1.6336167839966543E-4 0.0 0.06344967589043005 2.3959712831950932E-4 42-43 2.9405102111939777E-4 1.7425245695964312E-4 0.0 0.08490450965358612 2.3959712831950932E-4 44-45 3.593956924792639E-4 2.0692479263957622E-4 0.0 0.11234927162472991 2.3959712831950932E-4 46-47 3.9206802815919706E-4 2.178155711995539E-4 0.0 0.14498893496898307 2.50487906879487E-4 48-49 4.1384958527915244E-4 2.2870634975953163E-4 0.0 0.17930577821147278 2.6137868543946467E-4 50-51 4.1384958527915244E-4 2.3959712831950932E-4 0.0 0.21465724541716039 2.6137868543946467E-4 52-53 4.1384958527915244E-4 2.3959712831950932E-4 0.0 0.2513156060500453 2.940510211193978E-4 54-55 4.1384958527915244E-4 2.6137868543946467E-4 0.0 0.2968063880950721 3.049417996793755E-4 56-57 4.356311423991078E-4 2.6137868543946467E-4 0.0 0.35202263539415907 3.049417996793755E-4 58-59 4.356311423991078E-4 2.6137868543946467E-4 0.0 0.4120852791524361 3.049417996793755E-4 60-61 4.356311423991078E-4 2.831602425594201E-4 0.0 0.47553495504286614 3.049417996793755E-4 62-63 4.574126995190632E-4 2.940510211193978E-4 0.0 0.5422736460584094 3.049417996793755E-4 64-65 4.574126995190632E-4 3.049417996793755E-4 0.0 0.6214822785251273 3.049417996793755E-4 66-67 4.574126995190632E-4 3.049417996793755E-4 0.0 0.716820154039172 3.049417996793755E-4 68-69 4.574126995190632E-4 3.049417996793755E-4 0.0 0.8234299853627935 3.049417996793755E-4 70-71 4.574126995190632E-4 3.049417996793755E-4 0.0 0.9442522827071862 3.2672335679933087E-4 72-73 4.574126995190632E-4 3.049417996793755E-4 0.0 1.0739614553565207 3.376141353593086E-4 74-75 4.574126995190632E-4 3.049417996793755E-4 0.0 1.2203661915383006 3.7028647103924163E-4 76-77 4.574126995190632E-4 3.049417996793755E-4 0.0 1.3894019655677146 3.9206802815919706E-4 78-79 4.900850351989963E-4 3.049417996793755E-4 0.0 1.5817331149369207 3.9206802815919706E-4 80-81 5.663204851188402E-4 3.049417996793755E-4 0.0 1.7937439011640066 4.029588067191748E-4 82-83 5.663204851188402E-4 3.049417996793755E-4 0.0 2.023866052136335 4.1384958527915244E-4 84-85 5.772112636788179E-4 3.049417996793755E-4 0.0 2.276532114727818 4.1384958527915244E-4 86-87 5.881020422387955E-4 3.2672335679933087E-4 0.0 2.5586141702098 4.247403638391301E-4 88-89 5.881020422387955E-4 3.2672335679933087E-4 0.0 2.8786505889733043 4.356311423991078E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5810 0.0 40.399567 1 GGTATCA 3510 0.0 38.017334 1 ATCAACG 6805 0.0 36.01228 3 TCAACGC 7070 0.0 34.729633 4 CAACGCA 7250 0.0 33.801132 5 AACGCAG 7625 0.0 32.198963 6 TATCAAC 7345 0.0 31.825233 2 ACGCAGA 8850 0.0 27.635328 7 GTACATG 11875 0.0 26.678062 1 CGCAGAG 9440 0.0 25.907557 8 TACATGG 12600 0.0 24.8758 2 ACATGGG 12960 0.0 24.809196 3 CATGGGG 10985 0.0 21.401007 4 GCAGAGT 11935 0.0 20.251326 9 AGAGTAC 10375 0.0 17.69043 10-11 TATAAGC 2055 0.0 17.333189 3 ATGGGGA 7010 0.0 17.275942 5 CAGAGTA 10705 0.0 17.233812 10-11 GCTTATA 1890 0.0 17.015982 1 TTATAGG 2020 0.0 15.517494 3 >>END_MODULE