FastQCFastQC Report
Fri 27 May 2016
SRR940214_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940214_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4591040
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA38500.038.376061
GTATCAA64850.037.7274931
ATCAACG72300.032.5875783
TCAACGC72700.032.4736184
CAACGCA75200.031.3926735
AACGCAG78750.030.0978186
TATCAAC80650.029.5087992
ACGCAGA93050.025.4723637
GTACATG123100.024.1588941
CGCAGAG100600.023.7492738
TACATGG127500.022.5777472
ACATGGG129700.021.7548033
CATGGGG108550.019.341994
GCAGAGT123700.019.0838959
GTGGTAT23900.017.8897931
GCTTATA20650.017.7146031
AGAGTAC106300.017.53826910-11
CAGAGTA110400.016.8008910-11
TTATAGG20150.016.7375533
TATAAGC20500.016.4517923
Produced by FastQC (version 0.11.5)