FastQCFastQC Report
Fri 27 May 2016
SRR940213_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR940213_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4285620
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35100.041.4144361
GTATCAA57650.038.6465761
ATCAACG65750.033.0869333
TCAACGC67250.032.489824
CAACGCA67650.032.0164875
AACGCAG72050.030.1941876
TATCAAC71750.030.1887362
ACGCAGA84450.025.7044567
GTACATG110800.024.8671321
CGCAGAG90000.024.0137928
TACATGG115700.023.0730462
ACATGGG118700.022.4090823
CATGGGG100850.020.3464934
GTATAAG9700.020.0793481
GCAGAGT110350.019.154669
GCTTATA20850.018.4551011
CAGAGTA98300.017.63777710-11
AGAGTAC94350.017.21823910-11
ATGGGGA64050.015.9439535
GAGTACA89550.015.7553611
Produced by FastQC (version 0.11.5)